Modeling dysbiosis of human NASH in mice: Loss of gut microbiome diversity and overgrowth of Erysipelotrichales DOI Creative Commons
James K. Carter, Dipankar Bhattacharya,

Joshua N. Borgerding

et al.

PLoS ONE, Journal Year: 2021, Volume and Issue: 16(1), P. e0244763 - e0244763

Published: Jan. 4, 2021

Background & aim Non-alcoholic steatohepatitis (NASH) is a severe form of non-alcoholic fatty liver disease (NAFLD) that responsible for growing fraction cirrhosis and cancer cases worldwide. Changes in the gut microbiome have been implicated NASH pathogenesis, but lack suitable murine models has barrier to progress. We therefore characterized well-validated model establish its value modeling human disease. Methods The composition intestinal microbiota was monitored mice on 12- or 24-week protocol consisting high fat, sugar Western Diet (WD) plus once weekly i.p injection low-dose CCl 4 . Additional were subjected WD-only -only conditions assess independent effect these variables microbiome. Results There substantial remodeling mice, by declines both species diversity bacterial abundance. Based changes beta diversity, from clustered separately controls principal coordinate analyses. A comparison between with identified WD as primary driver early microbiome, resulting loss within 1 st week. signature emerged progressively at weeks 6 12, including, most notably, reproducible bloom Firmicute order Erysipelotrichales Conclusions established valuable study role microbes NASH, enabling us identify new signature.

Language: Английский

Vitamin D alleviates non-alcoholic fatty liver disease via restoring gut microbiota and metabolism DOI Creative Commons
Xiaolei Zhang, Lei Chen, Yang Jiang

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 2, 2023

Non-alcoholic fatty liver disease (NAFLD) represents a severe public health problem. Dysbiosis of gut microbiome has been identified as one the key environmental factors contributing to NAFLD. As an essential nutrition, Vitamin D (VD) plays important role in regulating microbiota based on its receptor (Vitamin Receptor, VDR) which is highly expressed gastrointestinal tract.Rats were fed with HFD (high-fat diet) for 12 weeks. And rats treated VD two times week by intraperitoneal injection H&E staining combined plasma biochemical index was performed characterize pathological changes and function liver. Fecal 16S rRNA gene sequencing metabolomics taken reveal altered metabolites.The alleviates HFD-induced lipid accumulation well decreases levels amlodipine besylate (ALT) aspartate (AST). supplement decreased ratio phylum Firmicutes/Bacteroidetes (F/B) but increased alpha diversity. In addition, treatment improved increasing Prevotella Porphyromonadaceae decreasing Mucispirillum, Acetatifactor, Desulfovibrio, Oscillospira abundance. Furthermore, capability tyrosine metabolism, tryptophan arginine biosynthesis, sphingolipid metabolism enhanced after treatment. Consistently, positively correlated metabolism. Importantly, abundance associated serotonin, melatonin, tryptamine, L-arginine, 3-dehydrosphinganine synthesize from tryptophan, tyrosine, arginosuccinate, serine, respectively.VD inhibited NAFLD accompany dysbiosis metabolites, suggesting that could be potential intervention used targeting specific microbiota.

Language: Английский

Citations

38
The Pathophysiological Associations Between Obesity, NAFLD, and Atherosclerotic Cardiovascular Diseases DOI

Meng Li,

Man Cui,

Guoxia Li

et al.

Hormone and Metabolic Research, Journal Year: 2024, Volume and Issue: 56(10), P. 683 - 696

Published: March 12, 2024

Abstract Obesity, non-alcoholic fatty liver disease (NAFLD), and atherosclerotic cardiovascular diseases are common growing public health concerns. Previous epidemiological studies unfolded the robust correlation between obesity, NAFLD, diseases. Obesity is a well-known risk factor for both of them can markedly increase odds On other hand, significant weight loss achieved by lifestyle modification, bariatric surgery, or medications, such as semaglutide, concomitantly improve NAFLD Therefore, certain pathophysiological links involved in development obesity NAFLD. Moreover, recent indicated that simultaneously targeting several mechanisms tirzepatide retatrutide leads to greater improves complications metabolic syndrome. These findings remind importance mechanistic viewpoint breaking association In this review article, we mainly focus on shared mechanisms, including insulin resistance, dyslipidemia, GLP1 signaling, inflammation, oxidative stress, mitochondrial dysfunction, gut dysbiosis, renin-angiotensin-aldosterone system (RAAS) overactivity, endothelial dysfunction. Most these alterations primarily initiated obesity. The further exacerbates molecular cellular alterations, leading progression final manifestation perturbation. A better insight into makes it feasible develop new multi-target approaches unhinge deleterious chain events linking

Language: Английский

Citations

11
Promising dawn in the management of pulmonary hypertension: The mystery veil of gut microbiota DOI Creative Commons
Yicheng Yang, Hanwen Zhang, Yaoyao Wang

et al.

iMeta, Journal Year: 2024, Volume and Issue: 3(2)

Published: Jan. 1, 2024

Abstract The gut microbiota is a complex community of microorganisms inhabiting the intestinal tract, which plays vital role in human health. It intricately involved metabolism, and it also affects diverse physiological processes. gut–lung axis bidirectional pathway between gastrointestinal tract lungs. Recent research has shown that microbiome crucial immune response regulation lungs development lung diseases. In this review, we present interrelated factors concerning associated metabolites pulmonary hypertension (PH), lethal disease characterized by elevated vascular pressure resistance. Our team explored gut‐microbiota‐derived cardiovascular diseases established correlation such as putrescine, succinate, trimethylamine N‐oxide (TMAO), N, N‐trimethyl‐5‐aminovaleric acid with Furthermore, found specific metabolites, TMAO betaine, have significant clinical value PH, suggesting their potential biomarkers management. detailing interplay microbiota, underscored therapeutic approaches modulating microbiota. Ultimately, endeavor to alleviate substantial socioeconomic burden disease. This review presents unique exploratory analysis link intending propel further investigations axis.

Language: Английский

Citations

9
Microbial metabolite delta-valerobetaine is a diet-dependent obesogen DOI
Ken Liu, Joshua A. Owens, Bejan Saeedi

et al.

Nature Metabolism, Journal Year: 2021, Volume and Issue: 3(12), P. 1694 - 1705

Published: Dec. 20, 2021

Language: Английский

Citations

47
Modeling dysbiosis of human NASH in mice: Loss of gut microbiome diversity and overgrowth of Erysipelotrichales DOI Creative Commons
James K. Carter, Dipankar Bhattacharya,

Joshua N. Borgerding

et al.

PLoS ONE, Journal Year: 2021, Volume and Issue: 16(1), P. e0244763 - e0244763

Published: Jan. 4, 2021

Background & aim Non-alcoholic steatohepatitis (NASH) is a severe form of non-alcoholic fatty liver disease (NAFLD) that responsible for growing fraction cirrhosis and cancer cases worldwide. Changes in the gut microbiome have been implicated NASH pathogenesis, but lack suitable murine models has barrier to progress. We therefore characterized well-validated model establish its value modeling human disease. Methods The composition intestinal microbiota was monitored mice on 12- or 24-week protocol consisting high fat, sugar Western Diet (WD) plus once weekly i.p injection low-dose CCl 4 . Additional were subjected WD-only -only conditions assess independent effect these variables microbiome. Results There substantial remodeling mice, by declines both species diversity bacterial abundance. Based changes beta diversity, from clustered separately controls principal coordinate analyses. A comparison between with identified WD as primary driver early microbiome, resulting loss within 1 st week. signature emerged progressively at weeks 6 12, including, most notably, reproducible bloom Firmicute order Erysipelotrichales Conclusions established valuable study role microbes NASH, enabling us identify new signature.

Language: Английский

Citations

45