Nature, Journal Year: 2020, Volume and Issue: 589(7840), P. 88 - 95
Published: Nov. 4, 2020
Language: Английский
Cell, Journal Year: 2018, Volume and Issue: 174(3), P. 730 - 743.e22
Published: July 1, 2018
Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping synaptic connectivity; however, the fly too conventional EM. We developed custom high-throughput EM platform imaged entire an adult female at resolution. To validate dataset, we traced brain-spanning circuitry involving mushroom body (MB), which been extensively studied its role in learning. All inputs to Kenyon cells (KCs), intrinsic neurons MB, were mapped, revealing previously unknown cell type, postsynaptic partners KC dendrites, unexpected clustering olfactory projection neurons. These reconstructions show that this freely available volume supports circuits, will significantly accelerate neuroscience. VIDEO ABSTRACT.
Language: Английский
Citations
985
Nature, Journal Year: 2017, Volume and Issue: 548(7666), P. 175 - 182
Published: Aug. 1, 2017
Language: Английский
Citations
523
Neuron, Journal Year: 2015, Volume and Issue: 88(6), P. 1086 - 1107
Published: Dec. 1, 2015
Language: Английский
Citations
506
Neuron, Journal Year: 2018, Volume and Issue: 98(2), P. 256 - 281
Published: April 1, 2018
Tremendous progress has been made since Neuron published our Primer on genetic dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical, neurophysiological, and perturbation studies have carried out in a multitude invertebrate vertebrate organisms, linking neurons to behavioral functions. New methods allow systematic classification cell types provide access diverse neuronal for connectivity coding during behavior. Here we evaluate key advances over the past decade discuss future directions.
Language: Английский
Citations
449
Science, Journal Year: 2019, Volume and Issue: 363(6424)
Published: Jan. 18, 2019
Optical and electron microscopy have made tremendous inroads toward understanding the complexity of brain. However, optical offers insufficient resolution to reveal subcellular details, lacks throughput molecular contrast visualize specific constituents over millimeter-scale or larger dimensions. We combined expansion lattice light-sheet image nanoscale spatial relationships between proteins across thickness mouse cortex entire Drosophila These included synaptic at dendritic spines, myelination along axons, presynaptic densities dopaminergic neurons in every fly brain region. The technology should enable statistically rich, large-scale studies neural development, sexual dimorphism, degree stereotypy, structural correlations behavior activity, all with contrast.
Language: Английский
Citations
361
eLife, Journal Year: 2020, Volume and Issue: 9
Published: Dec. 14, 2020
Making inferences about the computations performed by neuronal circuits from synapse-level connectivity maps is an emerging opportunity in neuroscience. The mushroom body (MB) well positioned for developing and testing such approach due to its conserved architecture, recently completed dense connectome, extensive prior experimental studies of roles learning, memory, activity regulation. Here, we identify new components MB circuit Drosophila, including visual input output neurons (MBONs) with direct connections descending neurons. We find unexpected structure sensory inputs, transfer information different modalities MBONs, modulation that dopaminergic (DANs). provide insights into circuitry used integrate outputs, between central complex inputs DANs, feedback MBONs. Our results a foundation further theoretical work.
Language: Английский
Citations
347
Nature, Journal Year: 2017, Volume and Issue: 546(7656), P. 101 - 106
Published: May 18, 2017
Language: Английский
Citations
335
eLife, Journal Year: 2016, Volume and Issue: 5
Published: March 17, 2016
Neuronal circuit mapping using electron microscopy demands laborious proofreading or reconciliation of multiple independent reconstructions. Here, we describe new methods to apply quantitative arbor and network context iteratively proofread reconstruct circuits create anatomically enriched wiring diagrams. We measured the morphological underpinnings connectivity in existing reconstructions Drosophila sensorimotor (larva) visual (adult) systems. Synaptic inputs were preferentially located on numerous small, microtubule-free 'twigs' which branch off a single microtubule-containing 'backbone'. Omission individual twigs accounted for 96% errors. However, synapses highly connected neurons distributed across twigs. Thus, robustness strong connection detailed twig anatomy was associated with reconstruction error. By comparing iterative consensus reconstructions, show that our method overcomes need redundant effort through discovery application relationships between cellular neuroanatomy synaptic connectivity.
Language: Английский
Citations
333
eLife, Journal Year: 2018, Volume and Issue: 7
Published: June 26, 2018
In most animals, the brain controls body via a set of descending neurons (DNs) that traverse neck. DN activity activates, maintains or modulates locomotion and other behaviors. Individual DNs have been well-studied in species from insects to primates, but little is known about overall connectivity patterns across population. We systematically investigated anatomy Drosophila melanogaster created over 100 transgenic lines targeting individual cell types. identified roughly half all comprehensively map between sensory motor neuropils nerve cord, respectively. find cord layered system reflecting fly's capability for two largely independent means -- walking flight using distinct sets appendages. Our results reveal basic functional pathways flies provide tools systematic interrogation neural circuits.
Language: Английский
Citations
321
Neuron, Journal Year: 2017, Volume and Issue: 96(4), P. 783 - 795.e4
Published: Oct. 26, 2017
Language: Английский
Citations
315