Bioprospecting hydroxylated chalcones in in vitro model of ischemia-reoxygenation and probing NOX4 interactions via molecular docking DOI
Arif Ali, Igor Moreira de Almeida, Emanuel Paula Magalhães

et al.

Biological Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Abstract Ischemia/reperfusion injury (I/R) is a leading cause of acute kidney (AKI) in conditions like transplants, cardiac surgeries, and nephrectomy, contributing to high global mortality morbidity. This study aimed analyze the protective effects 2′-hydroxychalcones treating I/R-induced AKI by targeting key pathological pathways. Considering strong antioxidant action along with other pharmacological roles chalcone derivatives, six were synthesized via Claisen-Schmidt condensation analyzed for their an I/R induced model using HK-2 cells. Among 2′-hydroxychalcones, A4 significantly increased cells viability compared group. Chalcone reduced cell death events reducing generation cytoplasmic ROS mitochondrial transmembrane potential. It also GSH SOD activity while TBARS levels, indicating action. Scanning electron microscope images showed that reversed morphological changes cells, including apoptotic blebbing fragmentation. Furthermore, silico studies revealed interactions NADPH oxidase 4, further supporting its role AKI. These results possess potential against cellular damage possibly due interaction NOX4 subunit oxidase.

Language: Английский

Ginsenoside Rg1 alleviates chronic inflammation-induced neuronal ferroptosis and cognitive impairments via regulation of AIM2 - Nrf2 signaling pathway DOI
Liangliang Kong, Yan Liu, Jingwei Li

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 330, P. 118205 - 118205

Published: April 17, 2024

Language: Английский

Citations

14
Natural products in traditional Chinese medicine: molecular mechanisms and therapeutic targets of renal fibrosis and state-of-the-art drug delivery systems DOI Creative Commons
Li Song, Wei Zhang,

Shiyun Tang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 170, P. 116039 - 116039

Published: Dec. 28, 2023

Renal fibrosis (RF) is the end stage of several chronic kidney diseases. Its series changes include excessive accumulation extracellular matrix, epithelial-mesenchymal transition (EMT) renal tubular cells, fibroblast activation, immune cell infiltration, and apoptosis. RF can eventually lead to dysfunction or even failure. A large body evidence suggests that natural products in traditional Chinese medicine (TCM) have great potential for treating RF. In this article, we first describe recent advances treatment by clarify their mechanisms action. They ameliorate disease phenotype, which includes apoptosis, endoplasmic reticulum stress, EMT, affecting relevant signaling pathways molecular targets, thereby delaying reversing fibrosis. We also present roles nanodrug delivery systems, been explored address drawback low oral bioavailability products. This may provide new ideas using treatment. Finally, insights into clinical prospects herbal

Language: Английский

Citations

19
New insight into oxidative stress and inflammatory responses to kidney stones: Potential therapeutic strategies with natural active ingredients DOI Open Access
Yue Sun,

Hongmei Sun,

Zhengze Zhang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117333 - 117333

Published: Sept. 8, 2024

Language: Английский

Citations

7
SOX9 Induces Orbital Fibroblast Activation in Thyroid Eye Disease Via MAPK/ERK1/2 Pathway DOI Creative Commons
Min Zhou,

Bingying Lin,

Pengsen Wu

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(2), P. 25 - 25

Published: Feb. 12, 2024

Purpose: To evaluate the expression of sry-box transcription factor 9 (SOX9) in orbital fibroblasts (OFs) thyroid eye disease (TED) and to find its potential role underlying mechanism fibrosis. Methods: OFs were cultured from connective tissues obtained patients with TED (n = 10) healthy controls 6). SOX9 was depleted by small interfering RNA or overexpressed through lentivirus transduction OFs. Fibroblast contractile activity measured collagen gel contraction assay proliferation examined EdU assay. Transcriptomic changes assessed sequencing. Results: The mRNA protein levels significantly higher than those controls. Extracellular matrix-related genes down-regulated knockdown up-regulated overexpression TED-OFs. decrease antiapoptotic ability OFs, whereas increased transformation, migration, suppressed phosphorylated ERK1/2, showed opposite effect. Epidermal growth receptor (EGFR) is one notably screened out Chromatin immunoprecipitation-qPCR demonstrated binding EGFR promoter. Conclusions: A high found can activate via MAPK/ERK1/2 signaling pathway, which turn promotes differentiation a downstream target gene SOX9. SOX9/EGFR be considered as therapeutic targets for treatment fibrosis TED.

Language: Английский

Citations

5
Targeting Pyroptosis: A Novel Strategy of Ginseng for the Treatment of Diabetes and Its Chronic Complications DOI
Ke Li,

Yajun Wang,

Chen Chen

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156430 - 156430

Published: Jan. 1, 2025

Language: Английский

Citations

0
Natural products in traditional Chinese medicine for renal fibrosis: a comprehensive review DOI Creative Commons
Qianqian Zhao, Meihua Jin, Qiang Zhao

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 16, 2025

Renal fibrosis represents the terminal pathological manifestation of most chronic kidney diseases, driving progressive loss renal function. Natural products have emerged as promising therapeutic agents for preventing and ameliorating due to their multi-target efficacy favorable safety profiles. In this review, we conducted a comprehensive literature search on PubMed using keywords “natural product” “renal fibrosis” from 2004 2025, identifying 704 relevant articles. We systematically categorize discuss biological effects key natural formulations with antifibrotic potential, focusing five major classes: glycosides, flavonoids, phenolic compounds, anthraquinones, terpenoids. Representative compounds each category are highlighted mechanisms action, including modulation oxidative stress, inflammation, autophagy, signaling pathways. This review aims provide theoretical foundation development product-based therapies combat fibrosis, offering insights into potential future research directions.

Language: Английский

Citations

0
Saponin monomers: Potential candidates for the treatment of type 2 diabetes mellitus and its complications DOI
Lvzhuo Zhang, Shifeng He, Lian Liu

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: 38(7), P. 3564 - 3582

Published: May 7, 2024

Abstract Type 2 diabetes mellitus (T2DM), a metabolic disease with persistent hyperglycemia primarily caused by insulin resistance (IR), has become one of the most serious health challenges 21st century, considerable economic and societal implications worldwide. Considering inevitable side effects conventional antidiabetic drugs, natural ingredients exhibit promising therapeutic efficacy can serve as safer more cost‐effective alternatives for management T2DM. Saponins are structurally diverse class amphiphilic compounds widely distributed in many popular herbal medicinal plants, some animals, marine organisms. There saponin monomers, such ginsenoside compound K, Rb1, Rg1, astragaloside IV, glycyrrhizin, diosgenin, showing great treatment T2DM its complications vivo vitro. However, although mechanisms action monomers at animal cell levels have been gradually elucidated, there is lack clinical data, which hinders development saponin‐based drugs. Herein, main factors/pathways associated comprehensive underlying potential applications these reviewed discussed, aiming to provide fundamental data future high‐quality studies trials.

Language: Английский

Citations

3
Red ginseng ameliorates lipotoxicity-induced renal fibrosis in hyperuricemia mice DOI

Yingling Zhang,

Si-Min Chen,

Yijie Song

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 327, P. 118014 - 118014

Published: March 7, 2024

Language: Английский

Citations

2
Ginsenoside Rg1 attenuates chronic inflammation-induced renal fibrosis in mice by inhibiting AIM2 inflammasome in an Nrf2-dependent manner DOI Creative Commons

Pengmin Ji,

Qifeng Shi,

Liangliang Kong

et al.

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 116, P. 106204 - 106204

Published: April 26, 2024

Chronic kidney disease (CKD) is a growing global public health issue. Ginsenoside Rg1 (Rg1) has favorable nephroprotective effects, but its role and mechanism in CKD need further investigation. Therefore, we observed the effect of on mouse kidneys after 21 days low-dose lipopolysaccharide (LPS) exposure. Meanwhile, investigated protective LPS-induced mesangial cells using Nrf2 inhibitor ML385. We found that chronic exposure to LPS caused fibrosis kidneys, accompanied by AIM2 activation oxidative stress imbalance; however, restored anomalies, Nrf2/HO-1 signaling. Finally, our vitro studies revealed Rg1′s effects could be inhibited ML385, can bind Nrf2, suggesting may involve then inhibition AIM2. Our study significant implications for prevention treatment diseases, particularly those related inflammation.

Language: Английский

Citations

2
Mitochondrial dysfunction in diabetic nephropathy: insights and therapeutic avenues from traditional Chinese medicine DOI Creative Commons

Dan-mai Zhao,

Rui Zhong, Xiaotian Wang

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: July 23, 2024

Diabetic nephropathy (DN) is a microvascular complication of diabetes mellitus. The progressive damage to glomeruli, tubules, and interstitium in the kidneys can lead development chronic kidney disease (CKD) end-stage renal (ESRD). Most energy we need comes from mitochondria. Mitochondria are best known as sites for production respiratory ATP essential eukaryotic life. pathogenesis DN involves variety factors, such altered haemodynamics, oxidative stress, inflammation, studies animal models suggest that mitochondrial dysfunction plays an important role DN. Traditional Chinese medicine (TCM) has history more than 2,500 years rich experience remarkable efficacy treatment Recent have found TCM may great potential regulating This review will elucidate main causes relationship with DN, explore depth mechanisms protect by improving dysfunction. Current pharmacological treatments patients do not prevent inevitable progression ESRD. With herbs, expected be most promising candidate continue learn about incorporate current advances extraction techniques.

Language: Английский

Citations

2