Recent progress of the research of metal-organic frameworks-molecularly imprinted polymers (MOFs-MIPs) in food safety detection field

Food Chemistry, Journal Year: 2024, Volume and Issue: 458, P. 140330 - 140330

Published: July 3, 2024

Language: Английский

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CRISPR/Cas System: The Accelerator for the Development of Non-nucleic Acid Target Detection in Food Safety

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(37), P. 13577 - 13594

Published: Sept. 1, 2023

Non-nucleic acid targets have posed a serious challenge to food safety. The detection of non-nucleic can enable us monitor contamination in timely manner. In recent years, the CRISPR/Cas system has been extensively explored biosensing. However, there is lack summary CRISPR/Cas-powered tailored involved This review comprehensively summarizes advances on construction and promising applications field safety related targets. current challenges futuristic perspectives are also proposed accordingly. rapidly evolving provided powerful propellant for target via integration with aptamer and/or DNAzyme. Compared traditional analytical methods, conceptually novel, essentially eliminates dependence large instruments, demonstrates capability rapid, accurate, sensitive, on-site testing.

Language: Английский

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Nanomaterials-assisted CRISPR/Cas detection for food safety: Advances, challenges and future prospects

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 167, P. 117269 - 117269

Published: Sept. 7, 2023

Language: Английский

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Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

Critical Reviews in Food Science and Nutrition, Journal Year: 2023, Volume and Issue: 64(32), P. 12061 - 12082

Published: Sept. 10, 2023

Food safety problems have become one of the most important public health issues worldwide. Therefore, development rapid, effective and robust detection is great importance. Amongst a range methods, nucleic acid isothermal amplification (NAIA) plays role in food detection. However, widespread application remains limited due to few shortcomings. CRISPR/Cas system has emerged as powerful tool detection, which could be readily integrated with NAIA improve sensitivity, specificity, adaptability versatility dependability. currently there was lack comprehensive summary regarding integration field In this review, recent advances based on CRISPR/Cas-integrated were comprehensively reviewed. To begin with, summarized. Then, types working principles introduced. The applications for mainly introduced objectively discussed. Lastly, current challenges future opportunities proposed. summary, technology expected an approach leading safer more reliable industry.

Language: Английский

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Nanolignin-containing cellulose nanofibrils (LCNF)-enabled multifunctional ratiometric fluorescent bio-nanocomposite films for food freshness monitoring

Food Chemistry, Journal Year: 2024, Volume and Issue: 453, P. 139673 - 139673

Published: May 16, 2024

Language: Английский

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Machine Learning-Assisted, Dual-Channel CRISPR/Cas12a Biosensor-In-Microdroplet for Amplification-Free Nucleic Acid Detection for Food Authenticity Testing

ACS Nano, Journal Year: 2024, Volume and Issue: 18(49), P. 33505 - 33519

Published: Dec. 2, 2024

The development of novel detection technology for meat species authenticity is imperative. Here, we developed a machine learning-supported, dual-channel biosensor-in-microdroplet platform named CC-drop (CRISPR/Cas12a digital single-molecule microdroplet biosensor). This strategy allowed us to quickly identify and analyze animal-derived components in foods. biosensor was enabled by CRISPR/Cas12a-based fluorescence lighting-up detection, the nucleic acid signals can be recognized Cas12a–crRNA binary complex trigger trans-cleavage any by-stander reporter single-stranded (ss) DNA, which converted amplified fluorescent readouts. ultralocalized reactor constructed reducing reaction volume from up picoliter accommodate aforementioned further enhance sensitivity even render an amplification-free detection. Moreover, established smartphone App coupled with random forest learning model based on parameters such as area, intensity, counting number ensure accuracy image recording processing. sample-to-result time within 80 min. Importantly, proposed able accurately detect ND1 (pork-specific) IL-2 (duck-specific) genes deep processed meat-derived foods that usually had truncated results were more robust practical than conventional real-time polymerase chain after side-by-side comparison. All all, expected used rapid food other detections future.

Language: Английский

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CRISPR/Cas12a triggered SERS and naked eye dual-mode biosensor for ultrasensitive and on-site detection of nucleic acid via cascade signal amplification

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 404, P. 135249 - 135249

Published: Jan. 2, 2024

Language: Английский

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Nanozyme-assisted CRISPR/Cas systems as an emerging platform for food safety applications: Recent advances

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113270 - 113270

Published: March 1, 2025

Language: Английский

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Hydrogel-integrated sensors for food safety and quality monitoring: Fabrication strategies and emerging applications

Critical Reviews in Food Science and Nutrition, Journal Year: 2023, Volume and Issue: 64(18), P. 6395 - 6414

Published: Jan. 20, 2023

Food safety is a global issue in public hygiene. The accurate, sensitive, and on-site detection of various food contaminants performs significant implications. However, traditional methods suffer from the time-consuming professional operation, restricting their application. Hydrogels with merits highly porous structure, high biocompatibility, good shape-adaptability, stimuli-responsiveness offer promising biomaterial to design sensors for ensuring safety. This review describes emerging applications hydrogel-based inspection recent years. In particular, this study elaborates on fabrication strategies unique sensing mechanisms depending whether hydrogel stimuli-responsive or not. Stimuli-responsive hydrogels can be integrated functional ligands sensitive convenient via signal amplification transduction; while non-stimuli-responsive are mainly used as solid-state encapsulating carriers probe, nanomaterial, cell conductive media. addition, existing challenges, future perspectives, application prospects discussed. These practices greatly enrich scenarios improve performance detection.

Language: Английский

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Cu(II)-induced magnetic resonance tuning and enhanced magnetic relaxation switching immunosensor for sensitive detection of chlorpyrifos and Salmonella

Food Chemistry, Journal Year: 2024, Volume and Issue: 446, P. 138847 - 138847

Published: Feb. 25, 2024

Language: Английский

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