Synthesis and characterization of silver nanoparticles developed using a novel lipopeptide(s) biosurfactant and evaluating its antimicrobial and cytotoxic efficacy.

Process Biochemistry, Journal Year: 2022, Volume and Issue: 124, P. 51 - 62

Published: Nov. 8, 2022

Language: Английский

---

Effects of Sub-Minimum Inhibitory Concentrations of Imipenem and Colistin on Expression of Biofilm-Specific Antibiotic Resistance and Virulence Genes in Acinetobacter baumannii Sequence Type 1894

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(20), P. 12705 - 12705

Published: Oct. 21, 2022

Antibiotics at suboptimal doses promote biofilm formation and the development of antibiotic resistance. The underlying molecular mechanisms, however, were not investigated. Here, we report effects sub-minimum inhibitory concentrations (sub-MICs) imipenem colistin on genes associated with biofilm-specific resistance in a multidrug-tolerant clinical strain Acinetobacter baumannii Sequence Type (ST) 1894. Comparative transcriptome analysis was performed untreated treated sub-MIC colistin. RNA sequencing data showed that 78 285 differentially expressed colistin-treated cells, respectively. Among (DEGs), 48 197 upregulated exclusively included those encoding matrix synthesis (pgaB), multidrug efflux pump (novel00738), fimbrial proteins, homoserine lactone synthase (AbaI). Upregulation biofilm-associated might enhance when sub-MICs antibiotics. downregulated include DNA gyrase (novel00171), 30S ribosomal protein S20 (novel00584), ribosome releasing factor (RRF) cells Downregulation these affects synthesis, which turn slows down cell metabolism makes more tolerant to In this investigation, also found 5 138 small RNAs (sRNAs) regardless treatment or not. Of these, sRNA00203 highest expression levels biofilm. sRNAs regulate gene are formation, may affect summary, exposed imipenem, coordinated responses result increased production, expression, slowdown metabolism, leads drug tolerance Targeting antibiotic-induced repressed represents new strategy for innovative effective treatments infections caused by A. baumannii.

Language: Английский

---

Antimicrobial Peptides in Infectious Diseases and Beyond—A Narrative Review

Life, Journal Year: 2023, Volume and Issue: 13(8), P. 1651 - 1651

Published: July 28, 2023

Despite recent medical research and clinical practice developments, the development of antimicrobial resistance (AMR) significantly limits therapeutics for infectious diseases. Thus, novel treatments diseases, especially in this era increasing AMR, are urgently needed. There is ongoing on non-classical therapies diseases utilizing alternative mechanisms to fight pathogens, such as bacteriophages or peptides (AMPs). AMPs evolutionarily conserved molecules naturally produced by several organisms, plants, insects, marine mammals, aiming protect host fighting pathogenic microorganisms. regarding developing use Moreover, have other non-medical applications food industry, preservatives, animal husbandry, plant protection, aquaculture. This review focuses AMPs, their origins, biology, structure, action, applications,

Language: Английский

---

Metal-free g-C3N5 photocatalysts with defect sites as efficient peroxymonosulfate activators for antibiotic removal in aqueous media

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(3), P. 112860 - 112860

Published: April 27, 2024

Language: Английский

---

Green synthesized silver nanoparticles from Phoenix dactylifera synergistically interact with bioactive extract of Punica granatum against bacterial virulence and biofilm development

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 192, P. 106708 - 106708

Published: May 21, 2024

Language: Английский

---

BL02, a phage against carbapenem- and polymyxin-B resistant Klebsiella pneumoniae, isolated from sewage: A preclinical study

Virus Research, Journal Year: 2023, Volume and Issue: 331, P. 199126 - 199126

Published: April 29, 2023

The emergence of Carbapenem-resistant Klebsiella pneumoniae (CRKP) represents a threat to public health. Polymyxin-B is generally considered last-resort antibiotic. In this study, we isolated carbapenem- and polymyxin-B resistant K. phage BL02 for the first time in Southwestern China evaluated its biological characteristics whole-genome sequence. pneumoniae, (CK02), was from blood male with severe septic shock, screened purified hospital sewage. could lyse 40 out 46 CRKP isolates (86.96%) has high activity pH range 6-10 temperature 4-55 °C. latency period about 10 min lysis 50 min. genome results showed that linear dsDNA total length 175,595 bp GC content 41.83%. A 275 ORFs were predicted no tRNA, rRNA, drug resistance genes, or virulence genes found genome. Phylogenetic analysis belongs family Straboviridae. Treatment infected mice two antibiotics (tigecycline ceftazidime/avibactam) resulted 7-day survival rates 28.57% 42.86%, respectively. contrast, rate single-dose BL02-treated group 71.43%. summary, preclinical study capable lysing validated safety efficacy an vivo model, which provides reference further research on controlling MDR pathogens.

Language: Английский

---

Key parameters for enhancing visible-light-driven photocatalytic activity of ZnS nanoparticle

Journal of Cleaner Production, Journal Year: 2023, Volume and Issue: 420, P. 138374 - 138374

Published: Aug. 7, 2023

Language: Английский

---

A novel acid-responsive polymer coating with antibacterial and antifouling properties for the prevention of biofilm-associated infections

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 239, P. 113939 - 113939

Published: May 2, 2024

Language: Английский

---

In-vitro antibacterial and antibiofilm activities and in-silico analysis of a potent cyclic peptide from a novel Streptomyces sp. strain RG-5 against antibiotic-resistant and biofilm-forming pathogenic bacteria

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(11)

Published: Oct. 30, 2024

Language: Английский

---

Characterization of the genome editing with miniature DNA nucleases TnpB and IscB in Escherichia coli strains

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Feb. 19, 2025

DNA endonucleases TnpB and IscB are emerging candidates for combating drug-resistant bacteria, particularly Escherichia coli, due to their specificity in targeting smaller size. However, the genome-editing of TnpB/IscB E. coli remains unclear. This study characterized genome editing different strains. First, toxicity cleavage results indicated was effective only MG1655, whereas enIscB demonstrated functionality ATCC9637/BL21(DE3). Subsequently, a tool established MG1655 by using (as thermophilic programmable endonuclease), achieving up 100% efficiency, while IscB/enIscB achieved Additionally, plasmids were successfully cured. Finally, mechanism underlying escape during elucidated. Overall, this applied TnpB/IscB/enIscB which will expand genetic manipulation toolbox facilitate development antimicrobial drugs. Characterization capabilities miniature proteins various strains provides insights into escaping mechanisms with IscB.

Language: Английский

---