Functional Characterization of a Highly Efficient Endoglucanase from Bacillus licheniformis BJ2022 and Its Application in the Preparation of Low-Molecular-Weight Konjac Glucomannan DOI
Tianxiang Zhu, Songlin Liu, Yu Gao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Endoglucanases may not only act on β-1,4-linkages in β-glucan but also target d-glucose glucomannan, which can expand their utility the preparation of prebiotics. In this study, a highly efficient endoglucanase (BlGH5) from Bacillus licheniformis BJ2022 was expressed and characterized. BlGH5 exhibited highest activity at pH 7.0 60 °C. It maintained over 80% 4.0-7.0 30-60 specifically cleaved β-1,4-glycosidic bonds linked to d-glucose. Site-directed mutagenesis results suggested that Arg91, Asn167, Glu168, Trp206, His228, Tyr230, Ser263, Trp290 are key residues for its binding catalytic activity. Moreover, displayed high against konjac glucomannan (KGM), indicating could be used prepare low-molecular-weight (KGM-L). Based physicochemical properties KGM KGM-L, KGM-L characterized by reduced molecular weight viscosity. Fecal fermentation experiments demonstrated promote production short-chain fatty acids (SCFAs). Still, more conducive growth gut probiotics. conclusion, we identified an degradation KGM. Results indicated would have superior prebiotic effects. Thus, our study provides basis future development application as prebiotic.

Language: Английский

Quorum Sensing of Bifidobacteria: Research and Progress DOI
Huahui Li, Bin He,

Ning Ma

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: 294, P. 128102 - 128102

Published: Feb. 13, 2025

Language: Английский

Citations

2
The gut microbiota–brain axis in neurological disorders DOI Creative Commons

Mingming You,

Nan Chen,

Yuanyuan Yang

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(8)

Published: July 20, 2024

Abstract Previous studies have shown a bidirectional communication between human gut microbiota and the brain, known as microbiota–gut–brain axis (MGBA). The MGBA influences host's nervous system development, emotional regulation, cognitive function through neurotransmitters, immune modulation, metabolic pathways. Factors like diet, lifestyle, genetics, environment shape composition together. Most research explored how regulates host physiology its potential in preventing treating neurological disorders. However, individual heterogeneity of microbiota, strains playing dominant role diseases, interactions these microbial metabolites with central/peripheral systems still need exploration. This review summarizes driving neurodevelopmental disorders (autism spectrum disorder attention deficit/hyperactivity disorder), neurodegenerative diseases (Alzheimer's Parkinson's disease), mood (anxiety depression) recent years discusses current clinical preclinical microbe‐based interventions, including dietary intervention, probiotics, prebiotics, fecal transplantation. It also puts forward insufficient on provides framework for further

Language: Английский

Citations

15
Gut microbiota and metabolites as predictors of biologics response in inflammatory bowel disease: A comprehensive systematic review DOI
Chen Wang, Yu Gu, Qiao Chu

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 282, P. 127660 - 127660

Published: Feb. 28, 2024

Language: Английский

Citations

11
Gut microbiota and its metabolic products in acute respiratory distress syndrome DOI Creative Commons
Dongwei Zhang,

Jiali Lu,

Bi-Ying Dong

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 16, 2024

The prevalence rate of acute respiratory distress syndrome (ARDS) is estimated at approximately 10% in critically ill patients worldwide, with the mortality ranging from 17% to 39%. Currently, ARDS usually higher COVID-19, giving another challenge for treatment. However, treatment efficacy far satisfactory. relationship between gut microbiota and has been substantiated by relevant scientific studies. not only changes distribution microbiota, but also influences intestinal mucosal barrier through alteration microbiota. modulation can impact onset progression triggering dysfunctions inflammatory response immune cells, oxidative stress, cell apoptosis, autophagy, pyroptosis, ferroptosis mechanisms. Meanwhile, may influence metabolic products In this review, we focus on how further function, cellular functions related signaling pathways during ARDS. roles microbiota-derived metabolites development occurrence are discussed.

Language: Английский

Citations

9
Gut microbiota and their metabolites in the immune response of rheumatoid arthritis: Therapeutic potential and future directions DOI
Xiaoyu Cai,

Fujia Ren,

Yao Yao

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 147, P. 114034 - 114034

Published: Jan. 12, 2025

Language: Английский

Citations

1
Gut Microbiota–Tumor Microenvironment Interactions: Mechanisms and Clinical Implications for Immune Checkpoint Inhibitor Efficacy in Cancer DOI Creative Commons

Sawsan Sudqi Said,

Wisam Nabeel Ibrahim

Cancer Management and Research, Journal Year: 2025, Volume and Issue: Volume 17, P. 171 - 192

Published: Jan. 1, 2025

Cancer immunotherapy has transformed cancer treatment in recent years, with immune checkpoint inhibitors (ICIs) emerging as a key therapeutic approach. ICIs work by inhibiting the mechanisms that allow tumors to evade detection. Although have shown promising results, especially solid tumors, patient responses vary widely due multiple intrinsic and extrinsic factors within tumor microenvironment. Emerging evidence suggests gut microbiota plays pivotal role modulating at site may even influence outcomes patients receiving ICIs. This review explores complex interactions between microenvironment, examining how these could impact effectiveness of ICI therapy. Furthermore, we discuss dysbiosis, an imbalance composition, contribute resistance ICIs, highlight microbiota-targeted strategies potentially overcome this challenge. Additionally, studies investigating diagnostic potential profiles patients, considering microbial markers might aid early detection stratification By integrating insights from preclinical clinical studies, aim shed light on microbiome modulation adjunct tool, paving way for personalized approaches optimize outcomes.

Language: Английский

Citations

1
Keystone Bacteria Dynamics in Chronic Obstructive Pulmonary Disease (COPD): Towards Differential Diagnosis and Probiotic Candidates DOI Creative Commons

Azadeh KavianFar,

Hamidreza Taherkhani, Hossein Lanjanian

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42719 - e42719

Published: Feb. 1, 2025

Preventing exacerbations in Chronic Obstructive Pulmonary Disease (COPD) is crucial due to the high mortality rate and associated costs of hospitalization for patients during exacerbations. Despite proven influence lung microbiome on disease control, dynamics bacterial communication different stages COPD remain unknown. This study aimed propose a group candidate bacteria differential diagnosis states based relative abundance correlation sputum samples. We compared data collected from 101 stable exacerbation states, as well 124 healthy controls two separate general cohorts, determine major keystone genera. To validate our findings, we utilized additional distinct public datasets, each comprising 81 subjects 87 condition, exacerbation, post-treatment phases. During Porphyromonas, Clostridium, Moryella, Megasphaera were identified phenotype-specific genera, while Prevotella, Streptococcus, Haemophilus, Veillonella consistently present across all datasets core members. Changes genera indicate rewiring interactions, with increased network connectivity observed dysbiosis more severe COPD. Bifidobacterium showed probiotic potential, positively correlating Lactobacillus Neisseria Haemophilus abundance, negatively correlated key bacteria. These findings promising potential simultaneous use along therapeutic prevent health, underscoring need further research future clinical studies.

Language: Английский

Citations

1
Fluoride induces pyroptosis via IL-17A-mediated caspase-1/11-dependent pathways and Bifidobacterium intervention in testis DOI
Ao Cheng,

Huifeng Luo,

Bingchao Fan

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 926, P. 172036 - 172036

Published: March 28, 2024

Language: Английский

Citations

7
Bifidobacterium exopolysaccharides: new insights into engineering strategies, physicochemical functions, and immunomodulatory effects on host health DOI Creative Commons
Mahsa Sadeghi, Babak Haghshenas, Yousef Nami

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 6, 2024

Bifidobacteria are a prominent type of bacteria that have garnered significant research attention for their exceptional probiotic properties and capacity to produce exopolysaccharides (EPSs). These compounds exhibit diverse physical, chemical, biological characteristics, prompting numerous investigations into potential applications. Researchers noted beneficial effects as immune modulators within the host’s body across various industries. Extensive has been conducted on immunomodulatory bifidobacteria-derived EPSs, with emerging engineering strategies aimed at enhancing immune-modulating capabilities. Understanding structure, physicochemical properties, activities these is crucial effective utilization different Our review encompassed studies exploring Bifidobacterium its metabolites, including sectors, drawing from databases. The distinctive EPSs spurred applications, revealing bolster system, combat inflammation, treat ailments. Additionally, possess antioxidant antimicrobial making them suitable incorporation range products spanning food, health, medicine.

Language: Английский

Citations

7
Advances in bio-polymer coatings for probiotic microencapsulation: chitosan and beyond for enhanced stability and controlled release DOI Creative Commons
Great Iruoghene Edo, Alice Njolke Mafe,

Nawar. F. Razooqi

et al.

Designed Monomers & Polymers, Journal Year: 2024, Volume and Issue: 28(1), P. 1 - 34

Published: Dec. 31, 2024

This review paper analyzes recent advancements in bio-polymer coatings for probiotic microencapsulation, with a particular emphasis on chitosan and its synergistic combinations other materials. Probiotic microencapsulation is essential protecting probiotics from environmental stresses, enhancing their stability, ensuring effective delivery to the gut. The begins an overview of highlighting significance safeguarding through processing, storage, gastrointestinal transit. Advances chitosan-based encapsulation are explored, including integration bio-polymers such as alginate, gelatin, pectin, well application nanotechnology innovative techniques like spray drying layer-by-layer assembly. Detailed mechanistic insights integrated, illustrating how influences gut microbiota by promoting beneficial bacteria suppressing pathogens, thus role prebiotic or synbiotic. Furthermore, delves into chitosan's immunomodulatory effects, particularly context inflammatory bowel disease (IBD) autoimmune diseases, describing immune signaling pathways influenced linking changes improvements systemic immunity. Recent clinical trials human studies assessing efficacy chitosan-coated presented, alongside discussion practical applications comparison vitro vivo findings highlight real-world relevance. sustainability sources impact addressed, along novel concept gut-brain axis. Finally, emphasizes future research needs, development personalized therapies exploration techniques.

Language: Английский

Citations

5