Methods in Ecology and Evolution,
Journal Year:
2024,
Volume and Issue:
15(6), P. 1084 - 1096
Published: April 22, 2024
Abstract
Efforts
to
characterize
microbial
life
across
diverse
environments
have
progressed
tremendously,
yet
the
microbiome
of
Earth's
largest
biomass
reservoir—the
wood
living
trees—has
been
largely
unexplored.
Current
understanding
tree
is
confined
roots
and
leaves,
with
little
attention
given
endophytic
wood,
even
though
emergent
studies
indicated
this
zone
as
a
niche
for
unique
taxa,
consequence
ecosystem
health
global
biogeochemical
cycles.
The
lack
investigation
derives
partly
from
physical
recalcitrance
which
presents
challenges
during
sampling,
homogenization,
extraction
nucleic
acids.
In
response
these
issues,
we
present
an
optimized
method
processing
use
in
analyses,
sampling
through
downstream
analyses.
Using
methane‐cycling
taxa
model
endophytes,
assess
losses
recovery
our
method,
determine
limit‐of‐detection
approximately
500
cells
per
100
mg
(dry)
wood.
For
all
six
species
evaluated—which
represented
several
hardwoods
softwoods—PCR
inhibition
proved
minimal,
expect
be
applicable
majority
species.
methods
presented
herein
can
facilitate
future
into
ecology
methane
cycling.
Water Research,
Journal Year:
2024,
Volume and Issue:
262, P. 121989 - 121989
Published: June 22, 2024
Wastewater
serves
as
an
important
reservoir
of
antimicrobial
resistance
(AMR),
and
its
surveillance
can
provide
insights
into
population-level
trends
in
AMR
to
inform
public
health
policy.
This
study
compared
two
common
high-throughput
screening
approaches,
namely
(i)
quantitative
PCR
(HT
qPCR),
targeting
73
genes,
(ii)
metagenomic
sequencing.
Weekly
composite
samples
wastewater
influent
were
taken
from
47
treatment
plants
(WWTPs)
across
Wales,
part
a
national
programme,
alongside
4
weeks
daily
effluent
large
municipal
hospital.
Metagenomic
analysis
provided
more
comprehensive
resistome
coverage,
detecting
545
genes
the
targeted
by
HT
qPCR.
It
further
contextual
information
critical
risk
assessment
(i.e.
potential
bacterial
hosts).
In
contrast,
qPCR
exhibited
higher
sensitivity,
quantifying
all
including
those
clinical
relevance
present
at
low
abundance.
When
limited
target
both
methods
able
reflect
spatiotemporal
dynamics
complete
resistome,
distinguishing
that
hospital
WWTPs.
Both
approaches
revealed
correlations
between
compositional
shifts
environmental
variables
like
ammonium
concentration,
though
differed
their
interpretation
some
influencing
factors.
Overall,
metagenomics
provides
profiling,
while
permits
sensitive
quantification
significant
resistance.
We
highlight
importance
selecting
appropriate
methodologies
aligned
aims
guide
development
effective
wastewater-based
monitoring
programmes.
Infection and Drug Resistance,
Journal Year:
2025,
Volume and Issue:
Volume 18, P. 1339 - 1354
Published: March 1, 2025
Abstract:
Klebsiella
spp
.
are
ubiquitous
gram-negative
bacteria,
commonly
present
in
natural
environments
and
as
part
of
the
human
microbiota.
is
involved
occurrence
development
many
diseases,
effective
antibiotics
for
it
have
attracted
attention
researchers.
In
recent
years,
its
multi-drug
resistance,
particularly
to
carbapenems
β-lactam
antibiotics,
has
posed
significant
challenges
clinical
treatment.
Therefore,
a
comprehensive
understanding
resistance
mechanisms
,
along
with
enhancement
detection
methods,
crucial
effectively
controlling
spread
drug-resistant
strains
guiding
individualized
This
paper
systematically
reviews
epidemiological
characteristics,
mechanisms,
treatment
strategies
aiming
provide
new
insights
management
this
pathogen.
Keywords:
drug
method,
β-lactam,
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 15, 2025
Abstract
Background
Campylobacter
infections
pose
a
significant
challenge
in
low-
and
middle-income
countries,
contributing
to
child
mortality.
is
linked
acute
gastrointestinal
illness
severe
long-term
consequences,
including
environmental
enteric
dysfunction
(EED)
stunting.
In
2018,
our
cross-sectional
study
Ethiopia
detected
88%
of
stools
from
children
aged
12–15
months,
with
an
average
11
species
per
stool
using
meta-total
RNA
sequencing.
Building
on
these
findings,
we
conducted
longitudinal
(December
2020–June
2022)
investigate
colonization
infants
identify
reservoirs
risk
factors
rural
eastern
Ethiopia.
Results
After
preliminary
screening
15
species-specific
quantitative
PCR,
analyzed
four
target
2,045
samples
(first
month
just
one
year
life)
biannual
mothers,
siblings,
livestock
(goats,
cattle,
sheep,
chickens).
Candidatus
C.
infans
(41%),
C.
jejuni
(26%),
upsaliensis
(13%)
were
identified
as
the
predominant
infant
gut.
Colonization
infans
C.jejuni
increased
(C.
infans:
0.85%,
jejuni-0.98%
increase/
day
odds
colonization)
abundance
(P
=
0.027,
0.024)
age.
Enteric
symptoms
strongly
associated
(diarrhea:
OR
2.02
[95%CI:
35%,100%];
fever:
1.62
14%,
83%])
2.29
46%,100%],
2.53
56%,100%]).
Based
linear
mixed
models,
found
elevated
cumulative
loads
load
(especially
females
1.5
10%,
67%]),
consuming
raw
milk
(OR
2.3
24%,100%])
or
those
exposed
areas
contaminated
animal
droppings
1.6
7%,93%]),
while
higher
ingesting
soil
feces
2.2
23%,100%]).
was
also
prevalent
siblings
(56%)
mothers
(45%),
whereas
common
chickens
(38%)
small
ruminants
(goats
27%,
sheep
21%).
Conclusions
highly
Ethiopian
infants.
primarily
human
hosts,
mainly
zoonotic
sources.
Our
findings
emphasize
need
for
targeted
interventions
addressing
environmental,
dietary,
behavioral
reduce
transmission
resource-limited
settings.
Environmental Research,
Journal Year:
2023,
Volume and Issue:
246, P. 118052 - 118052
Published: Dec. 30, 2023
The
Nordic
countries
(Denmark,
Finland,
Iceland,
Norway,
and
Sweden)
have
effectively
kept
lower
antibiotic-resistant
bacterial
(ARB)
pathogen
rates
than
many
other
countries.
However,
in
recent
years,
these
five
encountered
a
rise
ARB
cases
challenges
treating
infections
due
to
the
growing
prevalence
of
pathogens.
Wastewater-based
surveillance
(WBS)
is
valuable
supplement
clinical
methods
for
surveillance,
but
there
lack
comprehensive
understanding
WBS
application
This
review
aims
compile
latest
state-of-the-art
developments
monitoring
compare
them
with
practices.
After
reviewing
1480
papers
from
primary
search,
54
were
found
relevant,
15
additional
WBS-related
included.
Among
69
studies
analyzed,
42
dedicated
epidemiology,
while
27
focused
on
wastewater
monitoring.
PRISMA
literature
revealed
that
focus
four
major
objectives
ARB:
assessing
human
population,
identifying
evading
treatment,
quantifying
removal
rates,
evaluating
potential
evolution
during
treatment
process.
In
both
contexts,
most
studied
targets
pathogens
producing
carbapenemase
extended-spectrum
beta-lactamase
(ESBL),
primarily
Escherichia
coli
Klebsiella
spp.
vancomycin-resistant
Enterococcus
(VRE)
methicillin-resistant
Staphylococcus
aureus
(MRSA)
received
more
attention
epidemiology
studies,
probably
their
detection
wastewater.
Clinical
has
mostly
used
culturing,
antibiotic
susceptibility
testing,
genotyping,
employed
PCR-based
metagenomics
alongside
culture-based
techniques.
Imported
resulting
international
travel
hospitalization
abroad
appear
frequently
contributed
similarities
between
(e.g.,
knowledge
exchange
practices,
usage
patterns,
current
landscape)
could
facilitate
collaborative
efforts
developing
implementing
population-level
screening.
The Science of The Total Environment,
Journal Year:
2023,
Volume and Issue:
907, P. 167671 - 167671
Published: Oct. 7, 2023
Antimicrobial
resistance
(AMR)
can
be
transferred
to
humans
through
food
and
fresh
produce
an
ideal
vector,
as
it
is
often
consumed
raw
or
minimally
processed.
The
production
environment
of
the
agricultural
practices
regulations
vary
substantially
worldwide,
consequently,
contamination
sources
AMR.
In
this
study,
75
imported
non-imported
samples
purchased
from
Swiss
retailers
were
tested
for
presence
antimicrobial
resistant
bacteria
(ARB)
genes
(ARGs).
Moreover,
plasmidome
4
selected
was
sequenced
have
insight
on
diversity
mobile
resistome.
total,
91
ARB
isolated
produce,
mainly
cephalosporin-resistant
Enterobacterales
(n
=
64)
carbapenem-resistant
P.
aeruginosa
13).
All
aeruginosa,
well
16
Enterobacterales'
isolates
multidrug-resistant.
No
differences
between
found
regarding
number
ARB.
95
%
at
least
one
ARG
detected,
being
most
frequent
sul1,
blaTEM,
ermB.
Abundance
sul1
intI1
correlated
strongly
with
total
amount
ARGs,
suggesting
they
could
good
indicators
AMR
in
produce.
Furthermore,
fecal
marker
yccT,
indicating
that
gene
sulI
significantly
higher
samples,
anthropogenic
chain
analyses
coriander
carrot
revealed
several
ARGs
conferring
antiseptics
disinfectants
genetic
elements.
Overall,
study
demonstrated
contributes
dissemination
The ISME Journal,
Journal Year:
2024,
Volume and Issue:
18(1)
Published: Jan. 1, 2024
The
microbiome
of
the
built
environment
comprises
bacterial,
archaeal,
fungal,
and
viral
communities
associated
with
human-made
structures.
Even
though
most
these
microbes
are
benign,
antibiotic-resistant
pathogens
can
colonize
emerge
indoors,
creating
infection
risk
through
surface
transmission
or
inhalation.
Several
studies
have
catalogued
microbial
composition
ecology
in
different
types.
These
informed
vitro
that
seek
to
replicate
physicochemical
features
promote
pathogenic
survival
transmission,
ultimately
facilitating
development
validation
intervention
techniques
used
reduce
pathogen
accumulation.
Such
interventions
include
using
Bacillus-based
cleaning
products
on
surfaces
integrating
bacilli
into
printable
materials.
Though
this
work
is
its
infancy,
early
research
suggests
potential
use
biocontrol
hospital-
home-acquired
multidrug-resistant
infections.
Although
hold
promise,
there
an
urgent
need
better
understand
environments
determine
how
solutions
alter
species
interactions.
This
review
covers
our
current
understanding
proposes
strategies
translate
knowledge
effective
pathogens.
Environmental Microbiome,
Journal Year:
2024,
Volume and Issue:
19(1)
Published: March 15, 2024
Anthropogenic
activities
significantly
contribute
to
the
dissemination
of
antibiotic
resistance
genes
(ARGs),
posing
a
substantial
threat
humankind.
The
development
methods
that
allow
robust
ARG
surveillance
is
long-standing
challenge.
Here,
we
use
city-scale
monitoring
ARGs
by
using
two
most
promising
cutting-edge
technologies,
digital
PCR
(dPCR)
and
metagenomics.
BMC Research Notes,
Journal Year:
2025,
Volume and Issue:
18(1)
Published: Jan. 7, 2025
The
World
Health
Organization
(WHO)
has
declared
antimicrobial
resistance
(AMR)
as
one
of
the
top
threats
to
global
public
health.
While
AMR
surveillance
human
clinical
isolates
is
well-established
in
many
countries,
increasing
threat
intensified
efforts
detect
antibiotic
genes
(ARGs)
accurately
and
sensitively
environmental
samples,
wastewater,
animals,
food.
Using
five
ARGs
16S
rRNA
gene,
we
compared
quantitative
PCR
(qPCR)
metagenomic
sequencing
(MGS),
two
commonly
used
methods
uncover
wastewater
resistome.
We
both
by
evaluating
ARG
detection
through
a
municipal
treatment
chain.
Our
results
demonstrate
that
qPCR
was
more
sensitive
than
MGS,
particularly
diluted
samples
with
low
concentrations
such
oxidation
pond
water.
However,
MGS
potentially
specific
less
risk
off-target
binding
concentrated
raw
sewage.
analysis
revealed
multiple
subtypes
each
gene
which
could
not
be
distinguished
qPCR;
these
varied
across
different
sample
types.
findings
affect
conclusions
can
drawn
when
comparing
types,
terms
inferring
removal
rates
or
origins
genes.
conclude
appear
suitable
profile
resistome
other
depending
on
research
question
type
sample.
Sustainability,
Journal Year:
2023,
Volume and Issue:
15(13), P. 10640 - 10640
Published: July 6, 2023
Power
generation
and
wastewater
treatment
are
two
great
challenges
for
sustainable
development.
Microbial
fuel
cells
(MFCs)
a
alternative
that
can
generate
bioelectricity
in
the
bioremediation
process
of
wastewater.
For
this
reason,
objective
research
was
to
through
double-chamber
microbial-combustion
cell
systems
from
Covicorti
Wastewater
Treatment
Plant
(PTARC)
anodic
chamber
electrogenic
bacteria
such
as
Stenotrophomonas
maltophilia,
Acinetobacter
bereziniae,
Achromobacteria
xylosoxidans
cathode
chamber,
respectively.
Measurements
voltage,
current,
power
density,
current
optical
density
biochemical
oxygen
demand
(BOD)
were
made.
In
addition,
metagenomic
analysis
sample
performed.
It
shown
MFC
with
A.
generated
highest
voltage
peak
(1.01
±
0.06
V)
on
day
24,
while
S.
maltophilia
value
(0.71
0.02
mA).
The
pH
levels
slightly
alkaline,
maximum
conductivity
presented
by
cerevisiae,
81
2
mS/cm
24.
On
other
hand,
195,493
4717
mW/m2
4987
A/cm2,
respectively,
obtained
xylosoxidans.
Finally,
identified
predominant
phyla
Proteobacteria
present
samples
capable
generating
electrical
energy
Bacillota,
Pseudomonadota,
Bacteroidota,
Actinomyketone,
Campylobacterota.
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: Aug. 4, 2023
Effective
infectious
disease
surveillance
in
high-risk
regions
is
critical
for
clinical
care
and
pandemic
preemption;
however,
few
diagnostics
are
available
the
wide
range
of
potential
human
pathogens.
Here,
we
conduct
unbiased
metagenomic
sequencing
593
samples
from
febrile
Nigerian
patients
collected
three
settings:
i)
population-level
individuals
presenting
with
symptoms
consistent
Lassa
Fever
(LF);
ii)
real-time
investigations
outbreaks
suspected
etiologies;
iii)
undiagnosed
clinically
challenging
cases.
We
identify
13
distinct
viruses,
including
second
third
documented
cases
blood-associated
dicistrovirus,
a
highly
divergent,
unclassified
dicistrovirus
that
name
2.
show
pegivirus
C
common
co-infection
LF
associated
lower
viral
loads
favorable
outcomes.
help
uncover
causes
as
yellow
fever
virus,
monkeypox
noninfectious
cause,
latter
ultimately
determined
to
be
pesticide
poisoning.
demonstrate
local,
Nigerian-driven
metagenomics
response
complex
public
health
scenarios
generates
accurate,
differential
diagnoses,
yielding
insights
inform
policy.