Decoupled evolution of the Sex Peptide gene family and Sex Peptide Receptor in Drosophilidae

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(3)

Published: Jan. 12, 2024

Across internally fertilising species, males transfer ejaculate proteins that trigger wide-ranging changes in female behaviour and physiology. Much theory has been developed to explore the drivers of protein evolution. The accelerating availability high-quality genomes now allows us test how these are evolving at fine taxonomic scales. Here, we use from 264 species chart evolutionary history Sex Peptide (SP), a potent regulator post-mating responses

Language: Английский

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Cell cycle variants during Drosophila male accessory gland development

G3 Genes Genomes Genetics, Journal Year: 2024, Volume and Issue: 14(7)

Published: April 29, 2024

Abstract The Drosophila melanogaster male accessory gland (AG) is a functional analog of the mammalian prostate and seminal vesicles containing two secretory epithelial cell types, termed main secondary cells. This tissue responsible for making secreting fluid proteins other molecules that contribute to successful reproduction. cells this are binucleate polyploid, due variant cycles include endomitosis endocycling during metamorphosis. Here, we provide evidence additional cycle variants in tissue. We show connected by ring canals form after penultimate mitosis, describe an post-eclosion endocycle required maturation dependent on juvenile hormone signaling. present D. AG undergo unique reprogramming throughout organ development results step-wise truncations culminating octoploid nuclei with under-replicated heterochromatin mature gland. propose as model study developmental hormonal temporal control terminally differentiating tissues.

Language: Английский

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The seminal odorant binding protein Obp56g is required for mating plug formation and male fertility in Drosophila melanogaster

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Dec. 21, 2023

In Drosophila melanogaster and other insects, the seminal fluid proteins (SFPs) male sex pheromones that enter female with sperm during mating are essential for fertility induce profound post-mating effects on physiology. The SFPs in D. taxa include several members of large gene family known as odorant binding (Obps). Work has shown some Obp genes highly expressed antennae can mediate behavioral responses to odorants, potentially by carrying these molecules receptors. These observations have led hypothesis Obps might act molecular carriers or compounds important fertility, though functional evidence any species is lacking. Here, we used genetics test role seven response (PMR). We found Obp56g required induction PMR, whereas six dispensable. males lacking fail form a plug mated female's reproductive tract, leading ejaculate loss reduced storage, likely due its expression ejaculatory bulb. also examined evolutionary history genes, studies documented rapid evolution turnover SFP across taxa. extensive lability copy number positive selection acting two Obp22a Obp51a. Comparative RNAseq data from tract multiple revealed shows high subset taxa, conserved head phylogeny. Together, suggest may been co-opted function over time.

Language: Английский

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cGAS-like receptors drive a systemic STING-dependent host response in Drosophila

Cell Reports, Journal Year: 2024, Volume and Issue: 43(12), P. 115081 - 115081

Published: Dec. 1, 2024

cGAS-like receptor (cGLR)-stimulator of interferon genes (STING) recently emerged as an important pathway controlling viral infections in invertebrates. However, its exact contribution at the organismal level remains uncharacterized. Here, we use STING::GFP knockin reporter Drosophila flies to document activation vivo. Four tissues strongly respond injection cyclic dinucleotide 3'2'- guanosine monophosphate-adenosine monophosphate (cGAMP): central nervous system, midgut, Malpighian tubules, and genital ducts. The pattern induction injected with 3'2'-cGAMP or infected by two viruses different tropism suggests that is induced a systemic signal produced virus-infected cells. Accordingly, ectopic expression cGLR2 fat body induces STING signaling remote cGLR1/2-dependent activity transferred females during mating. Furthermore, infection can alter sleep cGLR1/2- STING-dependent manner. Altogether, our results reveal host response Drosophila.

Language: Английский

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Population biology of accessory gland-expressed de novo genes in Drosophila melanogaster

Genetics, Journal Year: 2021, Volume and Issue: 220(1)

Published: Nov. 17, 2021

Abstract Early work on de novo gene discovery in Drosophila was consistent with the idea that many such genes have male-biased patterns of expression, including a large number expressed testis. However, there has been little formal analysis variation abundance and properties different tissues. Here, we investigate population biology recently evolved melanogaster accessory gland, somatic male tissue plays an important role female fertility post mating response females, using same collection inbred lines used previously to identify testis-expressed genes, thus allowing for direct cross comparisons these two tissues reproduction. Using RNA-seq data, candidate located annotated intergenic intronic sequence determine chromosomal location, abundance, coding capacity. Generally, find major differences between terms though other as transcript length distribution are more similar. We also explore regulatory mechanisms how may interact selection produce D.

Language: Английский

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Evolution and genetics of accessory gland transcriptome divergence between Drosophila melanogaster and D. simulans

Genetics, Journal Year: 2024, Volume and Issue: 227(2)

Published: March 22, 2024

Abstract Studies of allele-specific expression in interspecific hybrids have provided important insights into gene-regulatory divergence and hybrid incompatibilities. Many such investigations Drosophila used transcriptome data from complex mixtures many tissues or gonads, however, regulatory may vary widely among species, sexes, tissues. Thus, we lack sufficiently broad sampling to be confident about the general biological principles divergence. Here, seek fill some these gaps literature by characterizing evolution misexpression a somatic male sex organ, accessory gland, F1 between melanogaster D. simulans. The gland produces seminal fluid proteins, which play an role female fertility subject adaptive due male–male male–female interactions. We find that trans differences are relatively more abundant than cis, contrast most literature, though large effect-size rare. Seminal protein genes significantly elevated levels tend regulated through both cis limited (over- underexpression relative parents) this organ compared other studies. As previous studies, male-biased overrepresented misexpressed much likely underexpressed. ATAC-Seq show chromatin accessibility is correlated with species expression. This work identifies unique properties suggests importance tissue-specific

Language: Английский

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Male-derived transcripts isolated from the mated female reproductive tract in Drosophila melanogaster

G3 Genes Genomes Genetics, Journal Year: 2023, Volume and Issue: 13(11)

Published: Sept. 19, 2023

In species with internal fertilization, sperm, and seminal fluid are transferred from male to female during mating. While both sperm contain various types of molecules, including RNA, the role most these molecules in coordination fertilization or other possible functions is poorly understood. Drosophila, exosomes accessory gland, which produces fluid, females, but their potential cargoes have not been described. Moreover, while RNA composition has described several mammalian species, little work on this problem occurred Drosophila. Here we use single nucleotide polymorphism differences between males females a set highly inbred lines D. melanogaster, transcriptome data reproductive tract, testis, investigate origin, vs female, isolated 3 tract organs, receptacle spermatheca, store parovaria, does not. We find that mated carry male-derived transcripts many genes, those markers gland known proteins. Our observations also support idea intact can be storage organs.

Language: Английский

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Evolution of secondary cell number and position in the Drosophila accessory gland

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(10), P. e0278811 - e0278811

Published: Oct. 25, 2023

In animals with internal fertilization, males transfer gametes and seminal fluid during copulation, both of which are required for successful reproduction. Drosophila other insects, is produced in the paired accessory gland (AG), ejaculatory duct, bulb. The D. melanogaster AG has emerged as an important model system this component male reproductive biology. Seminal proteins proper storage use sperm by females, also critical establishing maintaining a suite short- long-term postcopulatory female physiological responses that promote success. composed two main cell types. majority cells, referred to responsible production many proteins. A minority about 4%, secondary cells. These restricted distal tip AG, may play especially role maintenance post-mating response. Many studies evolution have suggested evolve quickly, does transcriptome. Here, we investigate number position collection eight species spanning entire history genus. We document heretofore underappreciated rapid evolutionary rate these specialized raising several questions developmental, functional, significance variation.

Language: Английский

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Cell cycle variants duringDrosophilaaccessory gland development

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2019, Volume and Issue: unknown

Published: July 30, 2019

Abstract The Drosophila melanogaster accessory gland is a functional analog of the mammalian prostate containing two secretory epithelial cell types, termed main and secondary cells. This tissue responsible for making secreting seminal fluid proteins other molecules that contribute to successful reproduction. cells this are bi-nucleate polyploid, due variant cycles include endomitosis endocycling during metamorphosis. Here we provide evidence additional cycle variants in tissue. We show connected by ring canals form after penultimate mitosis describe an post-eclosion endocycle required maturation dependent on juvenile hormone signaling. present undergo unique reprogramming throughout organ development results step-wise truncations culminating octoploid nuclei with under-replicated heterochromatin mature gland. propose as model study developmental hormonal temporal control terminally differentiating tissues.

Language: Английский

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Male Reproductive Glands and Their Secretions in Insects

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

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