Oviduct extracellular vesicles protein content and their role during oviduct–embryo cross-talk

Reproduction, Journal Year: 2017, Volume and Issue: 154(3), P. 253 - 268

Published: June 20, 2017

Successful pregnancy requires an appropriate communication between the mother and embryo. Recently, exosomes microvesicles, both membrane-bound extracellular vesicles (EVs) present in oviduct fluid have been proposed as key modulators of this unique cross-talk. However, little is known about their content role during oviduct-embryo dialog. Given differences secretions by vivo vitro epithelial cells (OEC), we aimed at deciphering EVs protein from sources. Moreover, analyzed functional effect on embryo development. Our study demonstrated for first time substantial secretion/content. Mass spectrometry analysis identified 319 proteins EVs, which 186 were differentially expressed when compared (P < 0.01). Interestingly, 97 exclusively 47 only 175 common. Functional revealed involved sperm-oocyte binding, fertilization development, some them lacking EVs. showed that vitro-produced embryos able to internalize culture with a In increased blastocyst rate, extended survival over improved quality. provides characterization increasing our understanding gamete/embryo-oviduct interactions. results point promising tools improve development under conditions.

Language: Английский

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Extracellular Vesicles in Human Reproduction in Health and Disease

Endocrine Reviews, Journal Year: 2018, Volume and Issue: 39(3), P. 292 - 332

Published: Jan. 30, 2018

Extensive evidence suggests that the release of membrane-enclosed compartments, more commonly known as extracellular vesicles (EVs), is a potent newly identified mechanism cell-to-cell communication both in normal physiology and pathological conditions. This review presents about formation different EVs, their definitive markers cargo content reproductive physiological processes, capacity to convey information between cells through transfer functional protein genetic alter phenotype function recipient associated with biology. In male tract, epididymosomes prostasomes participate regulating sperm motility activation, capacitation, acrosome reaction. female follicular fluid, oviduct/tube, uterine cavity EVs are considered vehicles carry during oocyte maturation, fertilization, embryo-maternal crosstalk. via might be also involved triggering, maintenance, progression reproductive- obstetric-related pathologies such endometriosis, polycystic ovarian syndrome, preeclampsia, gestational diabetes, erectile dysfunction. this review, we provide current knowledge on present future use not only biomarkers, but therapeutic targeting agents, mainly vectors for drug or compound delivery into target tissues.

Language: Английский

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Tetraspanins, More than Markers of Extracellular Vesicles in Reproduction

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(20), P. 7568 - 7568

Published: Oct. 14, 2020

The participation of extracellular vesicles in many cellular processes, including reproduction, is unquestionable. Although currently, the tetraspanin proteins found are mostly applied as markers, increasing evidence points to their role vesicle biogenesis, cargo selection, cell targeting, and uptake under both physiological pathological conditions. In this review, we bring other insight into involvement physiology mammalian reproduction. We provide knowledge regarding tetraspanins these processes somatic cells. Furthermore, discuss future direction towards an understanding functions tissues fluids reproductive system gamete maturation, fertilization, embryo development; mutual contact communication complexity.

Language: Английский

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MicroRNA Signaling in Embryo Development

Biology, Journal Year: 2017, Volume and Issue: 6(3), P. 34 - 34

Published: Sept. 14, 2017

Expression of microRNAs (miRNAs) is essential for embryonic development and serves important roles in gametogenesis. miRNAs are secreted into the extracellular environment by embryo during preimplantation stage development. Several cell types secrete biological fluids environment. These fluid-derived have been shown to circulate body. Stable transport dependent on proper packaging vesicles (EVs), including exosomes. vesicles, which also contain RNA, DNA proteins, forefront research cell-to-cell communication. Interestingly, EVs identified many reproductive fluids, such as uterine fluid, where their miRNA content proposed serve a mechanism crosstalk between mother conceptus. Here, we review role molecular signaling discuss early implantation.

Language: Английский

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Effect of bovine oviductal extracellular vesicles on embryo development and quality in vitro

Reproduction, Journal Year: 2017, Volume and Issue: 153(4), P. 461 - 470

Published: Jan. 19, 2017

The aim of this study was to evaluate the effect extracellular vesicles (EV) from oviductal fluid (OF), either ampulla or isthmus, on development and quality in vitro -cultured bovine embryos. Zygotes were cultured synthetic oviduct (SOF + 3 mg/mL BSA) without calf serum (C− group), presence × 10 5 EV/mL ampullary isthmic OF at 1 4 g (10 K) (100 K), compared with SOF 5% FCS (C+ group). OF-EV size concentration assessed by electron microscopy nanotracking analysis system. Embryo recorded Days 7–9, blastocyst through cryotolerance gene expression analysis. Lower yield observed Day 7 C− groups (12.0–14.3%) C+ (20.6%); however, these differences compensated 8 9 (Day 9: 28.5–30.8%). Importantly, survival rate blastocysts produced 100 K higher than that group 72 h after vitrification warming (80.1 vs 34.5 50.5% respectively, P < 0.05). In terms expression, upregulated water channel AQP3 DNMT3A SNRPN transcripts C+, being intermediate. lipid receptor LDLR downregulated all other groups. conclusion, addition culture embryos absence improves produced.

Language: Английский

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Paracrine and endocrine actions of interferon tau (IFNT)

Reproduction, Journal Year: 2017, Volume and Issue: 154(5), P. F45 - F59

Published: Oct. 6, 2017

This review focuses on the paracrine and endocrine actions of interferon tau (IFNT) during pregnancy recognition establishment in ruminants. Pregnancy involves suppression endometrial luteolytic mechanism by conceptus to maintain progesterone production corpus luteum (CL). The antiluteolytic effects conceptus-derived IFNT inhibit upregulation oxytocin receptors epithelia uterus, thereby preventing prostaglandin F2 alpha (PGF2α) pulses. In endometrium, induces or upregulates a large number classical IFN-stimulated genes (ISGs) regulates expression many other cell-specific manner that are likely important for elongation, implantation pregnancy. Further, has extrauterine cells tissues. sheep, luteal resistance PGF2α, ensuring survival CL maintenance ISGs induced circulating peripheral blood mononuclear may also be useful as an indicator status cattle. An increased knowledge is improve reproductive efficiency

Language: Английский

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Extracellular vesicle mediated embryo-endometrial cross talk during implantation and in pregnancy

Journal of Assisted Reproduction and Genetics, Journal Year: 2018, Volume and Issue: 36(2), P. 189 - 198

Published: Oct. 25, 2018

Language: Английский

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Preparation and characterization of extracellular vesicles

American Journal of Reproductive Immunology, Journal Year: 2020, Volume and Issue: 85(2)

Published: Oct. 29, 2020

Abstract Extracellular vesicles (EVs) are heterogeneous membranous secreted by every cell type and offer significant potential in therapy diagnostics. Differential ultracentrifugation is the gold standard for EV isolation, although other techniques including, polyethylene glycol (PEG) precipitation, immunoprecipitation, size exclusion chromatography, immuno‐isolation approaches common. Purified EVs can be characterized based on their physical characteristics, biochemical composition, or of origin. For concentration measurement, nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), electron microscopy commonly employed methods. Biochemical analyses typically performed using flow cytometry, immunoblotting, proteomic investigation. Based tissue origin, have specific markers that used to isolate purify cell‐associated an affinity selection approach. Despite existence several methods isolation characterization, major limitations associated with each method hinder progress field. Evolving concepts biology possess great promise better characterization leading a insight biological function immense clinical implications. In this review, we discuss recent advancements approaches.

Language: Английский

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Intrauterine exosomes are required for bovine conceptus implantation

Biochemical and Biophysical Research Communications, Journal Year: 2017, Volume and Issue: 495(1), P. 1370 - 1375

Published: Nov. 29, 2017

Language: Английский

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Oviductal extracellular vesicles interact with the spermatozoon’s head and mid-piece and improves its motility and fertilizing ability in the domestic cat

Scientific Reports, Journal Year: 2019, Volume and Issue: 9(1)

Published: July 1, 2019

Fertilization and early embryo development are regulated by a unique maternal-gamete/embryo cross-talk within the oviduct. Recent studies have shown that extracellular vesicles (EVs) oviduct play important roles in mediating this developmental process. Here, we examined influence of oviductal EVs on sperm function domestic cat. We demonstrated (1) enriched proteins related to energy metabolism, membrane modification, reproductive function; (2) bound fused with membranes acrosome mid piece; (3) incubating improved motility, fertilizing capacity cat spermatozoa prevented acrosomal exocytosis vitro. These findings indicated mediate fertilization provides new insights improve cryopreservation vitro wild felids human.

Language: Английский

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