Spatial transcriptomics in adultDrosophilareveals new cell types in the brain and identifies subcellular mRNA patterns in muscles DOI Creative Commons
Jasper Janssens, Pierre Mangeol, Nikolai Hecker

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 6, 2023

Recently, we have achieved a significant milestone with the creation of Fly Cell Atlas. This single-nuclei atlas encompasses entire fly, covering head and body, in addition to all major organs. catalogs many hundreds cell types, which annotated 250. Thus, large number clusters remain be fully characterized, particular brain. Furthermore, by applying sequencing, information about spatial location cells body possible subcellular localization mRNAs within these is lost. Spatial transcriptomics promises tackle issues. In proof-of-concept study, here applied using selected gene panel pinpoint locations 150 mRNA species adult fly. enabled us map unknown identified Atlas their fly Additionally, discovered interesting principles transcriptional diversity crowded muscle that may spark future mechanistic investigations. present set computational tools will allow for easier integration single-cell datasets.

Language: Английский

Structural basis of odor sensing by insect heteromeric odorant receptors DOI
Jiawei Zhao, Andy Quaen Chen, Jae-Wook Ryu

et al.

Science, Journal Year: 2024, Volume and Issue: 384(6703), P. 1460 - 1467

Published: June 13, 2024

Most insects, including human-targeting mosquitoes, detect odors through odorant-activated ion channel complexes consisting of a divergent odorant-binding subunit (OR) and conserved co-receptor (Orco). As basis for understanding how odorants activate these heteromeric receptors, we report here cryo–electron microscopy structures two different odorant receptor containing ORs from disease-vector mosquitos Aedes aegypti or Anopheles gambiae . These reveal an unexpected stoichiometry one OR to three Orco subunits. Comparison in odorant-bound unbound states indicates that binding the sole is sufficient open pore, suggesting mechanism activation conceptual framework evolution insect sensitivity.

Language: Английский

Citations

28
Common principles for odour coding across vertebrates and invertebrates DOI

Kara A Fulton,

David Zimmerman,

Aravi Samuel

et al.

Nature reviews. Neuroscience, Journal Year: 2024, Volume and Issue: 25(7), P. 453 - 472

Published: May 28, 2024

Language: Английский

Citations

18
Sparse and stereotyped encoding implicates a core glomerulus for ant alarm behavior DOI Creative Commons
Taylor Hart,

Dominic D. Frank,

Lindsey E. Lopes

et al.

Cell, Journal Year: 2023, Volume and Issue: 186(14), P. 3079 - 3094.e17

Published: June 15, 2023

Ants communicate via large arrays of pheromones and possess expanded, highly complex olfactory systems, with antennal lobes in the brain comprising up to ∼500 glomeruli. This expansion implies that odors could activate hundreds glomeruli, which would pose challenges for higher-order processing. To study this problem, we generated transgenic ants expressing genetically encoded calcium indicator GCaMP sensory neurons. Using two-photon imaging, mapped complete glomerular responses four ant alarm pheromones. Alarm robustly activated ≤6 activity maps three inducing panic our species converged on a single glomerulus. These results demonstrate that, rather than using broadly tuned combinatorial encoding, employ precise, narrowly tuned, stereotyped representations The identification central hub glomerulus behavior suggests simple neural architecture is sufficient translate pheromone perception into behavioral outputs.

Language: Английский

Citations

24
Avoiding alkaline taste through ionotropic receptors DOI Creative Commons
Prakash Pandey,

Bhanu Shrestha,

Youngseok Lee

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 110087 - 110087

Published: May 23, 2024

Taste organs contain distinct gustatory receptors that help organisms differentiate between nourishing and potentially harmful foods. The detection of high pH levels plays a crucial role in food selection, but the specific responsible for perceiving elevated foods have remained unknown. By using

Language: Английский

Citations

7
Transcriptional and post-transcriptional control of odorant receptor choice in ants DOI Creative Commons
Anindita Brahma,

Dominic D. Frank,

P. Daniel H. Pastor

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(24), P. 5456 - 5466.e5

Published: Dec. 1, 2023

Insects and mammals have independently evolved odorant receptor genes that are arranged in large genomic tandem arrays. In mammals, each olfactory sensory neuron chooses to express a single stochastic process includes substantial chromatin rearrangements. Here, we show ants, which the largest repertoires among insects, employ different mechanism regulate gene expression from Using single-nucleus RNA sequencing, found ant neurons choose transcription start sites along an array but then produce mRNA many downstream genes. This can result transcripts dozens of receptors being present nucleus. Such rampant co-expression at first seems difficult reconcile with narrow tuning system. However, fluorescence situ hybridization showed only most upstream transcribed reach cytoplasm where it be translated into protein, whereas gets sequestered implies that, despite extensive genes, ultimately produces one or very few functional receptors. Evolution has thus molecular solutions insects convergent challenge selecting small subsets repertoires.

Language: Английский

Citations

15
Olfactory sensory neuron population expansions influence projection neuron adaptation and enhance odour tracking DOI Creative Commons
S. Takagi, Gizem Sancer,

Liliane Abuin

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 15, 2024

The evolutionary expansion of sensory neuron populations detecting important environmental cues is widespread, but functionally enigmatic. We investigated this phenomenon through comparison homologous olfactory pathways Drosophila melanogaster and its close relative sechellia, an extreme specialist for Morinda citrifolia noni fruit. D. sechellia has evolved species-specific expansions in select, noni-detecting (OSN) populations, multigenic changes. Activation inhibition defined proportions neurons demonstrate that OSN number increases contribute to stronger, more persistent, noni-odour tracking behaviour. These result increased synaptic connections with their projection (PN) partners, which are conserved between species. Surprisingly, having OSNs does not lead greater odour-evoked PN sensitivity or reliability. Rather, pooling exhibit reduced adaptation, likely weakened lateral inhibition. Our work reveals unexpected functional impact population explain ecologically-relevant,

Language: Английский

Citations

5
Multilayer regulation underlies the functional precision and evolvability of the olfactory system DOI Creative Commons
Jérôme Mermet, Steeve Cruchet,

Asfa Sabrin Borbora

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Sensory neurons must be reproducibly specified to permit accurate neural representation of external signals but also able change during evolution. We studied this paradox in the Drosophila olfactory system by establishing a single-cell transcriptomic atlas all developing antennal sensory lineages, including latent populations that normally undergo programmed cell death (PCD). This reveals transcriptional control is robust, imperfect, defining selective receptor expression. A second layer precision afforded intersection expression functionally-interacting subunits. third defined stereotyped PCD patterning, which masks promiscuous fated die and removes "empty" lacking receptors. Like choice, under lineage-specific control; promiscuity regulation leads previously-unappreciated heterogeneity neuronal numbers. Thus functional mature belies developmental noise might facilitate evolution pathways.

Language: Английский

Citations

0
Population-level morphological analysis of paired CO2- and odor-sensing olfactory neurons in D. melanogaster via volume electron microscopy DOI Creative Commons

Jonathan Choy,

Shadi Charara,

Kalyani Cauwenberghs

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

ABSTRACT Dendritic morphology is a defining characteristic of neuronal subtypes. In Drosophila , heterotypic olfactory receptor neurons (ORNs) expressing different receptors display diverse dendritic morphologies, but whether such diversity exists among homotypic ORNs remains unclear. Using serial block-face scanning electron microscopy on cryofixed tissues, we analyzed the majority CO 2 -sensing (ab1C) and their odor-sensing neighbors (ab1D) in D. melanogaster antenna. Surprisingly, ab1C featured flattened, sheet-like dendrites—distinct from cylindrical branches typical neurons—and displayed remarkable diversity, ranging plain sheets to tube-like structures that enclose several neighboring dendrites, forming “dendrite-within-dendrite” structures. Similarly, ab1D dendrites varied simple, unbranched forms numerously branched morphologies. These findings suggest morphological heterogeneity common even ORNs, potentially expanding functional adaptability ranges sensory physiological properties.

Language: Английский

Citations

0
Spatial transcriptomics in the adult Drosophila brain and body DOI Open Access
Jasper Janssens, Pierre Mangeol, Nikolai Hecker

et al.

Published: March 5, 2025

Recently, we have achieved a significant milestone with the creation of Fly Cell Atlas. This single-nuclei atlas encompasses entire fly, covering head and body, in addition to all major organs. catalogs many hundreds cell types, which annotated 250. Thus, large number clusters remain be fully characterized, particular brain. Furthermore, by applying sequencing, information about spatial location cells body possible subcellular localization mRNAs within these is lost. Spatial transcriptomics promises tackle issues. In proof-of-concept study, here applied using selected gene panel pinpoint locations 150 mRNA species adult fly. enabled us map unknown identified Atlas their fly Additionally, discovered interesting principles transcriptional diversity crowded muscle that may spark future mechanistic investigations. present set computational tools will allow for easier integration single-cell datasets.

Language: Английский

Citations

0
Spatial transcriptomics in the adult Drosophila brain and body DOI Creative Commons
Jasper Janssens, Pierre Mangeol, Nikolai Hecker

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: March 18, 2025

Recently, we have achieved a significant milestone with the creation of Fly Cell Atlas. This single-nuclei atlas encompasses entire fly, covering head and body, in addition to all major organs. catalogs many hundreds cell types, which annotated 250. Thus, large number clusters remain be fully characterized, particular brain. Furthermore, by applying sequencing, information about spatial location cells body possible subcellular localization mRNAs within these is lost. Spatial transcriptomics promises tackle issues. In proof-of-concept study, here applied using selected gene panel pinpoint locations 150 mRNA species adult fly. enabled us map unknown identified Atlas their fly Additionally, discovered interesting principles transcriptional diversity crowded muscle that may spark future mechanistic investigations. present set computational tools will allow for easier integration single-cell datasets.

Language: Английский

Citations

0