Scientific Reports,
Journal Year:
2022,
Volume and Issue:
12(1)
Published: June 1, 2022
Abstract
Recently,
a
new
Listeria
species,
“
swaminathanii
”,
was
proposed.
Here,
we
phenotypically
and
genotypically
characterize
two
additional
strains
that
were
previously
obtained
from
soil
samples
compare
the
results
to
type
strain.
Complete
genomes
for
both
assembled
hybrid
Illumina
Nanopore
sequencing
reads
annotated.
Further
genomic
analysis
including
average
nucleotide
identity
(ANI)
detection
of
mobile
genetic
elements
genes
interest
(e.g.,
virulence-associated)
conducted.
The
showed
98.7–98.8%
ANI
with
UTK
C1-0015
genome
contained
partial
monocin
locus
plasmid,
while
C1-0024
full
prophage.
Phenotypic
characterization
consistent
those
performed
on
proposed
strain
conducted
assess
consistency
phenotypes
across
greater
diversity
species
(n
=
3
instead
n
1).
Only
few
findings
notably
different
strain,
such
as
catalase
activity,
glycerol
metabolism,
starch
growth
at
41
°C.
This
study
further
expands
our
understanding
this
newly
sensu
stricto
species.
Veterinary Medicine and Science,
Journal Year:
2022,
Volume and Issue:
8(2), P. 735 - 740
Published: Jan. 18, 2022
Abstract
Listeriosis
is
one
of
the
most
common
nervous
diseases
in
ruminants,
and
caused
almost
exclusively
by
Gram‐positive
bacterium,
Listeria
monocytogenes
.
However,
there
are
few
reports
listeriosis
associated
with
L.
innocua
,
which
genetically
closely
related
to
but
considered
non‐pathogenic.
In
this
work,
we
report
two
cases
suppurative
meningoencephalitis
apparently
previously
healthy
ruminants
from
different
farms,
strains
were
recovered.
The
whole
genomes
both
isolates
sequenced,
allowing
phylogenetic
analyses
be
performed,
indicated
that
very
related.
Virulence
determinants
searched,
especially
genes
coding
for
main
virulence
factors
have
been
described
Surprisingly,
do
not
possess
such
determinants.
Instead,
carried
a
set
encode
other
genus
detected
using
Factor
Database
(VFDB):
iap
(division
invasion
host
cells),
lpeA
(entry
into
non‐professional
phagocytes
fbpA
(multifunctional
factor,
including
adherence
cells)
lspA
(surface
protein
anchoring),
lap
(adhesion
enterocytes
trans
epithelial
translocation)
pdgA
(resistance
lysozyme),
oatA
antimicrobial
compounds
also
required
growth
inside
macrophages)
lplA1
(use
host‐metabolites
vivo
growth),
gtcA
(catalyses
teichoic
acid
bacterial
wall),
prsA2
(cell
invasion,
vacuole
lysis
intracellular
clpC,
clpE
clpP
(survival
under
several
stress
conditions).
These
among
others
detected,
could
involved
ability
produce
damage
animal
human
hosts.
results
highlight
multifactorial
profile
pathogenesis
need
comprehensive
scientific
research
address
microbiological,
environmental
veterinary
aspects
listeriosis.
Microbiology Spectrum,
Journal Year:
2022,
Volume and Issue:
10(3)
Published: June 6, 2022
Soil
samples
collected
in
the
Great
Smoky
Mountains
National
Park
yielded
a
Listeria
isolate
that
could
not
be
classified
to
species
level.
Whole-genome
sequence-based
average
nucleotide
identity
BLAST
and
silico
DNA-DNA
Hybridization
analyses
confirmed
this
novel
sensu
stricto
with
highest
similarity
L.
marthii
(ANI
=
93.9%,
isDDH
55.9%).
Additional
whole-genome-based
analysis
using
Genome
Taxonomy
Database
Toolkit
further
supported
delineation
as
species,
tool
failed
assign
identification.
Phenotypic
genotypic
characterization
results
indicate
is
nonpathogenic.
Specifically,
described
here
phenotypically
(i)
nonhemolytic
(ii)
negative
for
phosphatidylinositol-specific
phospholipase
C
activity;
draft
genome
lacks
all
virulence
genes
found
pathogenicity
islands
1,
2,
3,
4
well
internalin
inlA
inlB.
While
type
strain
contains
an
apparently
intact
catalase
gene
(kat),
catalase-negative
(an
unusual
characteristic
species).
identified
nonsynonymous
mutation
conserved
codon
of
kat
likely
linked
phenotype.
Rapid
identification
systems,
including
two
biochemical
one
matrix-assisted
laser
desorption/ionization,
misidentified
either
monocytogenes,
innocua,
or
marthii.
We
propose
name
swaminathanii,
FSL
L7-0020T
(=ATCC
TSD-239T).
IMPORTANCEL.
swaminathanii
originated
from
US
it
will
first
date
without
official
standing
nomenclature.
Validation
was
impeded
by
Park's
requirements
access,
ultimately
deemed
too
restrictive
International
Committee
on
Systematics
Prokaryotes.
However,
lack
valid
status
should
detract
significance
adding
clade.
Notably,
detection
non-monocytogenes
food
processing
environment
conditions
facilitate
presence
pathogen
monocytogenes.
If
isolated,
our
data
show
potential
another
stricto,
notably
Therefore,
developers
spp.
methods,
who
historically
only
include
validly
published
their
validation
studies,
ensure
accurate
results.
ISME Communications,
Journal Year:
2023,
Volume and Issue:
3(1)
Published: Aug. 19, 2023
Comprehending
bacterial
genomic
variation
linked
to
distinct
environments
can
yield
novel
insights
into
mechanisms
underlying
differential
adaptation
and
transmission
of
microbes
across
environments.
Gaining
such
is
particularly
crucial
for
pathogens
as
it
benefits
public
health
surveillance.
However,
the
understanding
limited
by
a
scarcity
investigations
in
coupled
with
different
ecological
contexts.
To
address
this
limitation,
we
focused
on
Listeria,
an
important
genus
food
safety
that
includes
human
pathogen
L.
monocytogenes,
analyzed
large-scale
dataset
collected
us
from
natural
food-associated
United
States.
Through
comparative
genomics
analyses
449
isolates
soil
390
agricultural
water
produce
processing
facilities
representing
seeligeri,
innocua,
welshimeri,
find
profiles
strongly
differ
within
each
species.
This
supported
environment-associated
subclades
presence
plasmids,
stress
islands,
accessory
genes
involved
cell
envelope
biogenesis
carbohydrate
transport
metabolism.
Core
genomes
Listeria
species
are
also
associated
accurately
predict
isolation
sources
at
lineage
level
monocytogenes
using
machine
learning.
We
large
appears
be
jointly
driven
property,
climate,
land
use,
accompanying
species,
chiefly
Actinobacteria
Proteobacteria.
Collectively,
our
data
suggest
populations
have
genetically
adapted
environments,
which
may
limit
their
Pathogens,
Journal Year:
2023,
Volume and Issue:
12(8), P. 1062 - 1062
Published: Aug. 18, 2023
Whole-genome
sequencing
(WGS)
was
used
for
the
genomic
characterization
of
one
hundred
and
ten
strains
Listeria
innocua
(L.
innocua)
isolated
from
twenty-three
cattle
farms,
eight
beef
abattoirs,
forty-eight
retail
outlets
in
Gauteng
province,
South
Africa.
In
silico
multilocus
sequence
typing
(MLST)
to
identify
isolates’
types
(STs).
BLAST-based
analyses
were
antimicrobial
virulence
genes.
The
study
also
linked
detection
genes
origin
(industries
samples)
L.
isolates.
detected
14
STs,
13
resistance
genes,
23
Of
STs
detected,
ST637
(26.4%),
ST448
(20%),
537
(13.6%),
1085
(12.7%)
predominant,
frequency
varied
significantly
(p
<
0.05).
All
110
isolates
carriers
or
more
with
lin
(100%),
fosX
tet(M)
(30%)
being
most
frequently
recognized,
(clpC,
clpE,
clpP,
hbp1,
svpA,
hbp2,
iap/cwhA,
lap,
lpeA,
lplA1,
lspA,
oatA,
pdgA,
prsA2)
found
all
innocua.
Overall,
diversity
significant
differences
frequencies
resistance,
according
origins
(source
sample
type)
This,
first
recovered
three
levels/industries
(farm,
abattoir,
retail)
production
system
Africa,
provides
data
on
organism’s
distribution
potential
food
safety
implications.
