Molecular logic of neuromodulatory systems in the zebrafish telencephalon DOI Creative Commons
Lukas Anneser, Chie Satou, Hans-Rudolf Hotz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 5, 2023

Abstract The function of neuronal networks is determined not only by synaptic connectivity but also neuromodulatory systems that broadcast information via distributed connections and volume transmission. To understand the molecular constraints organize signaling in telencephalon adult zebrafish we used transcriptomics additional approaches to delineate cell types, determine their phylogenetic conservation, map expression marker genes at high granularity. combinatorial GPCRs type markers indicates all types are subject modulation multiple monoaminergic distinct combinations neuropeptides. Individual were associated with (typically >30) expressed a few diagnostic levels, suggesting different act combination albeit unequal weights. These results provide detailed brain areas telencephalon, identify core components networks, highlight type-specificity neuropeptides GPCRs, begin decipher logic neuromodulation.

Language: Английский

Neural signal propagation atlas of Caenorhabditis elegans DOI Creative Commons
Francesco Randi, Anuj Kumar Sharma, Sophie Dvali

et al.

Nature, Journal Year: 2023, Volume and Issue: 623(7986), P. 406 - 414

Published: Nov. 1, 2023

Abstract Establishing how neural function emerges from network properties is a fundamental problem in neuroscience 1 . Here, to better understand the relationship between structure and of nervous system, we systematically measure signal propagation 23,433 pairs neurons across head nematode Caenorhabditis elegans by direct optogenetic activation simultaneous whole-brain calcium imaging. We sign (excitatory or inhibitory), strength, temporal causal direction these create functional atlas. find that differs model predictions are based on anatomy. Using mutants, show extrasynaptic signalling not visible anatomy contributes this difference. identify many instances dense-core-vesicle-dependent signalling, including timescales less than second, evoke acute transients—often where no wired connection exists but relevant neuropeptides receptors expressed. propose that, such cases, extrasynaptically released serve similar classical neurotransmitters. Finally, our measured atlas predicts dynamics spontaneous activity do models conclude both synaptic drive short timescales, measurements evoked crucial for interpreting function.

Language: Английский

Citations

81
System-wide mapping of neuropeptide-GPCR interactions inC. elegans DOI Creative Commons
Isabel Beets,

Sven Zels,

Elke Vandewyer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 1, 2022

Summary Neuropeptides are ancient, widespread signaling molecules that underpin almost all brain functions. They constitute a broad ligand-receptor network, mainly by binding to G protein-coupled receptors (GPCRs). However, the organization of peptidergic network and roles many neuropeptides remain elusive, as our insight into neuropeptide-receptor interactions is limited peptide GPCRs in animal models humans still orphan receptors. Here we report genome-wide neuropeptide-GPCR interaction map C. elegans . By reverse pharmacology screening over 55,384 possible interactions, identify 461 cognate peptide-GPCR couples uncover with specific complex combinatorial encoded across within single genes. These provide insights neuropeptide functions evolution. Combining dataset phylogenetic analysis supports peptide-receptor co-evolution conservation at least 14 bilaterian systems This resource lays foundation for system-wide network. Highlights System-wide deorphanizes 68 Discovery pairs additional ligands characterized show receptor Peptide-GPCR support long-range expansion

Language: Английский

Citations

19
Molecular heterogeneity of C. elegans glia across sexes DOI Creative Commons
Maria D. Purice, Elgene J.A. Quitevis,

R. Sean Manning

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 24, 2023

A comprehensive description of nervous system function, and sex dimorphism within, is incomplete without clear assessment the diversity its component cell types, neurons glia. C. elegans has an invariant with first mapped connectome a multi-cellular organism single-cell atlas neurons. Here we present single nuclear RNA-seq evaluation glia across entire adult system, including both sexes. Machine learning models enabled us to identify sex-shared sex-specific glial subclasses. We have identified validated molecular markers in silico vivo for these subcategories. Comparative analytics also reveals previously unappreciated heterogeneity anatomically identical between within sexes, indicating consequent functional heterogeneity. Furthermore, our datasets reveal that while express neuropeptide genes, they lack canonical unc-31/CAPS-dependent dense core vesicle release machinery. Thus, employ alternate neuromodulator processing mechanisms. Overall, this atlas, available at www.wormglia.org, rich insights into animal

Language: Английский

Citations

9
The Logic of Transgenerational Inheritance: Timescales of Adaptation DOI Creative Commons
Titas Sengupta, Rachel Kaletsky, Coleen T. Murphy

et al.

Annual Review of Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 39(1), P. 45 - 65

Published: June 20, 2023

Myriad mechanisms have evolved to adapt changing environments. Environmental stimuli alter organisms' physiology create memories of previous Whether these environmental can cross the generational barrier has interested scientists for centuries. The logic passing on information from generation is not well understood. When it useful remember ancestral conditions, and when might be deleterious continue respond a context that may no longer exist? key found in understanding conditions trigger long-lasting adaptive responses. We discuss biological systems use conditions. Responses spanning different timescales employ molecular machineries result differences duration or intensity exposure. Understanding components multigenerational inheritance underlying beneficial maladaptive adaptations fundamental how organisms acquire transmit across generations.

Language: Английский

Citations

8
Pathogen infection induces sickness behaviors by recruiting neuromodulatory systems linked to stress and satiety inC. elegans DOI Creative Commons
Sreeparna Pradhan,

Gurrein K. Madan,

Di Kang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 5, 2024

SUMMARY When animals are infected by a pathogen, peripheral sensors of infection signal to the brain coordinate set adaptive behavioral changes known as sickness behaviors. While pathways that from periphery have been intensively studied in recent years, how central circuits reconfigured elicit behaviors is not well understood. Here we find neuromodulatory systems linked stress and satiety recruited upon drive C. elegans . Upon chronic bacterium Pseudomonas aeruginosa PA14, decrease their feeding behavior, then display reversible bouts quiescence, eventually die. The ALA neuron its neuropeptides FLP-7, FLP-24, NLP-8, which control stress-induced sleep uninfected animals, promote PA14-induced reduction. However, neuropeptide FLP-13 instead acts delay quiescence death animals. Cell-specific genetic perturbations show neurons release distinct ALA. A brain-wide imaging screen reveals infection-induced involves ASI DAF-7/TGF-beta, satiety-induced Our results suggest common neuromodulators across different physiological states, acting neural sources combinations state-dependent

Language: Английский

Citations

2
Competition between stochastic neuropeptide signals calibrates the rate of satiation DOI Open Access
Stephen X. Zhang, Angela Kim,

Joseph C. Madara

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 12, 2023

We investigated how transmission of hunger- and satiety-promoting neuropeptides, NPY αMSH, is integrated at the level intracellular signaling to control feeding. Receptors for these peptides use second messenger cAMP, but messenger's spatiotemporal dynamics role in energy balance are controversial. show that AgRP axon stimulation paraventricular hypothalamus evokes probabilistic spatially restricted release triggers stochastic cAMP decrements downstream MC4R-expressing neurons (PVH

Language: Английский

Citations

5
Chemical cognition: chemoconnectomics and convergent evolution of integrative systems in animals DOI
Leonid L. Moroz, Daria Y. Romanova

Animal Cognition, Journal Year: 2023, Volume and Issue: 26(6), P. 1851 - 1864

Published: Nov. 1, 2023

Language: Английский

Citations

4
A neurotransmitter atlas ofC. elegansmales and hermaphrodites DOI Creative Commons
Chen Wang,

Berta Vidal,

Surojit Sural

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 25, 2023

ABSTRACT Mapping neurotransmitter identities to neurons is key understanding information flow in a nervous system. It also provides valuable entry points for studying the development and plasticity of neuronal identity features. In C. elegans system, have been largely assigned by expression pattern analysis pathway genes that encode biosynthetic enzymes or transporters. However, many these assignments relied on multicopy reporter transgenes may lack relevant cis -regulatory therefore not provide an accurate picture usage. We analyzed patterns 16 CRISPR/Cas9-engineered knock-in strains all main types neurotransmitters (glutamate, acetylcholine, GABA, serotonin, dopamine, tyramine, octopamine) both hermaphrodite male. Our reveals novel sites systems within glia, as well non-neural cells. The resulting atlas defines be exclusively neuropeptidergic, substantially expands repertoire capable co-transmitting multiple neurotransmitters, identifies uptake monoaminergic neurotransmitters. Furthermore, we observed unusual co-expression synthesis genes, suggesting existence transmitters. results what constitutes most extensive whole-animal-wide map usage date, paving way better communication specification .

Language: Английский

Citations

3
A single neuron inC. elegansorchestrates multiple motor outputs through parallel modes of transmission DOI Creative Commons
Yung-Chi Huang,

Jinyue Luo,

Wenjia Huang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 2, 2023

Animals generate a wide range of highly coordinated motor outputs, which allows them to execute purposeful behaviors. Individual neuron classes in the circuits that behavior have remarkable capacity for flexibility, as they exhibit multiple axonal projections, transmitter systems, and modes neural activity. How these multi-functional properties neurons enable generation behaviors remains unknown. Here we show HSN

Language: Английский

Citations

2
Scaling Properties for Artificial Neural Network Models of a Small Nervous System DOI Creative Commons
Quilee Simeon, Leandro Venâncio, Michael Skuhersky

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 14, 2024

Abstract The nematode worm C. elegans provides a unique opportunity for exploring in silico data-driven models of whole nervous system, given its transparency and well-characterized system facilitating wealth measurement data from wet-lab experiments. This study explores the scaling properties that may govern learning underlying neural dynamics this small by using artificial network (ANN) models. We investigate accuracy self-supervised next time-step activity prediction as function For scaling, we report monotonic log-linear reduction mean-squared error (MSE) amount data. model find MSE to be nonlinear size ANN Furthermore, observe dataset are influenced particular choice architecture but not precise experimental source Our results fall short producing long-horizon predictive generative suggest directions achieve those. In our extrapolate recording more is fruitful near-term approach obtaining better system.

Language: Английский

Citations

0