Environmental Microbiology,
Journal Year:
2024,
Volume and Issue:
26(6)
Published: June 1, 2024
Abstract
Carbon‐fixing
micro‐organisms
(CFMs)
play
a
pivotal
role
in
soil
carbon
cycling,
contributing
to
uptake
and
sequestration
through
various
metabolic
pathways.
Despite
their
importance,
accurately
quantifying
the
absolute
abundance
of
these
soils
has
been
challenging.
This
study
used
digital
droplet
polymerase
chain
reaction
(ddPCR)
approach
measure
key
emerging
CFMs
pathways
fen
bog
at
different
depths,
ranging
from
0
15
cm.
We
targeted
total
prokaryotes,
oxygenic
phototrophs,
aerobic
anoxygenic
phototrophic
bacteria
chemoautotrophs,
optimizing
conditions
achieve
quantification
genes.
Our
results
revealed
that
phototrophs
were
most
abundant
CFMs,
making
up
15%
prokaryotic
abundance.
They
followed
by
chemoautotrophs
10%
9%.
observed
higher
gene
concentrations
than
bog.
There
also
variations
depth,
which
differed
between
for
all
findings
underscore
peatlands,
challenging
previous
estimates
relied
solely
on
microbial
dioxide
fixation
assessments.
Incorporating
is
essential
comprehensive
understanding
contributions
processes.
sheds
light
complex
mechanisms
functioning
peatlands.
Journal of Clinical Investigation,
Journal Year:
2023,
Volume and Issue:
133(10)
Published: March 28, 2023
BACKGROUND.
Lung
infections
are
among
the
most
consequential
manifestations
of
cystic
fibrosis
(CF)
and
associated
with
reduced
lung
function
shortened
survival.
Drugs
called
CFTR
modulators
improve
activity
dysfunctional
transmembrane
conductance
regulator
(CFTR)
channels,
which
is
physiological
defect
causing
CF.
However,
it
unclear
how
improved
affects
CF
infections.
Plants,
Journal Year:
2023,
Volume and Issue:
12(14), P. 2736 - 2736
Published: July 23, 2023
The
phytomicrobiome
plays
a
crucial
role
in
soil
and
ecosystem
health,
encompassing
both
beneficial
members
providing
critical
goods
services
pathogens
threatening
food
safety
security.
potential
benefits
of
harnessing
the
power
for
plant
disease
suppression
management
are
indisputable
interest
agriculture
but
also
forestry
landscaping.
Indeed,
diseases
can
be
mitigated
by
situ
manipulations
resident
microorganisms
through
agronomic
practices
(such
as
minimum
tillage,
crop
rotation,
cover
cropping,
organic
mulching,
etc.)
well
applying
microbial
inoculants.
However,
numerous
challenges,
such
lack
standardized
methods
microbiome
analysis
difficulty
translating
research
findings
into
practical
applications
at
stake.
Moreover,
climate
change
is
affecting
distribution,
abundance,
virulence
many
pathogens,
while
altering
functioning,
further
compounding
strategies.
Here,
we
will
first
review
literature
demonstrating
how
agricultural
have
been
found
effective
promoting
health
enhancing
suppressiveness
mitigation
shift
phytomicrobiome.
Challenges
barriers
to
identification
use
then
discussed
before
focusing
on
impacts
functioning
outcome.
The ISME Journal,
Journal Year:
2023,
Volume and Issue:
17(8), P. 1303 - 1314
Published: June 7, 2023
Abstract
Ecological
and
evolutionary
processes
simultaneously
regulate
microbial
diversity,
but
the
their
driving
forces
remain
largely
unexplored.
Here
we
investigated
ecological
characteristics
of
microbiota
in
hot
springs
spanning
a
broad
temperature
range
(54.8–80
°C)
by
sequencing
16S
rRNA
genes.
Our
results
demonstrated
that
niche
specialists
generalists
are
embedded
complex
interaction
dynamics.
On
thermal
tolerance
axis,
(T)
sensitive
(at
specific
temperature)
versus
T-resistant
least
five
temperatures)
species
were
characterized
different
breadth,
community
abundance
dispersal
potential,
consequently
differing
potential
trajectory.
The
niche-specialized
T-sensitive
experienced
strong
barriers,
leading
to
completely
shift
high
fitness
low
abundant
communities
at
each
(“home
niche”),
such
trade-offs
thus
reinforced
peak
performance,
as
evidenced
speciation
across
temperatures
increasing
diversification
with
temperature.
In
contrast,
advantageous
expansion
poor
local
shown
wide
breadth
extinction,
indicating
these
“jack-of-all-trades,
master-of-none”.
Despite
differences,
evolutionarily
interacted.
Specifically,
continuous
transition
from
insured
exclusion
probability
relatively
constant
level
temperatures.
co-evolution
co-adaptation
line
red
queen
theory.
Collectively,
our
findings
demonstrate
could
alleviate
environmental-filtering-induced
negative
effect
on
diversity.
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2025,
Volume and Issue:
15
Published: Feb. 26, 2025
Bloodstream
infections(BSIs)
caused
by
carbapenem-resistant
Acinetobacter
baumannii
(CRAB)
have
a
high
mortality
rate
due
to
the
levels
of
drug
resistance.
There
is
an
urgent
need
establish
sensitive
and
accurate
detection
method
rapidly
detect
CRAB
in
BSIs.
A
new
was
developed
based
on
fluorescence
quantitative
PCR
(qPCR)
targeting
specific
region
16sRNA
OXA-23
gene
from
CRAB.
The
parameters
were
evaluated
optimized.
This
qPCR
further
applied
AB
30
clinical
specimens.
established
this
study
showed
specificity.
successfully
differentiated
baumannii(A.
baumanii)
26
other
common
pathogens
BSIs
identify
carbapenem
resistance
gene.
shows
limit
(LOD)
3×10-3
ng/μL,
displays
good
linear
relationship
between
excellent
repeatability
(CV
≤2%).
results
for
specimens
using
are
complete
agreement
with
those
blood
culture
susceptibility
test.
has
strong
specificity,
wide
range,
repeatability,
lower
LOD
than
(Non-fluorescence
quantification).
provides
technical
support
early
diagnosis
Agronomy,
Journal Year:
2024,
Volume and Issue:
14(7), P. 1483 - 1483
Published: July 9, 2024
Rhizoctonia
solani
is
a
soil-borne
fungal
species
with
worldwide
distribution
and
poses
serious
threats
to
wide
range
of
economically
important
crops
such
as
grain
forage
crops.
This
pathogen
has
survival
capabilities
within
plants
soil,
giving
rise
sclerotia
persisting
for
several
months
or
years.
Alfalfa
(Medicago
sativa)
the
most
widely
grown
crop
in
China
worldwide.
The
unique
characteristics
alfalfa,
excellent
quality,
ruminant
desirability,
substantial
biomass
output,
distinguish
it
from
other
fodder
crops,
also
known
“Queen
Forages”.
However,
production
alfalfa
seriously
affected
by
R.
solani,
yield
losses
20%
60%
globally.
review
firstly
summarizes
diseases
seedling
damping-off,
root
rot,
crown
cankers,
stem
blight,
rot
caused
mechanism
this
pathogen.
techniques
detection
quantification
soils,
well
management
through
host
resistance,
cultural
practices,
fungicides,
biological
control,
were
then
overviewed.
provides
scientific
knowledge
enable
researchers
efficiently
manage
production.
Global Change Biology,
Journal Year:
2024,
Volume and Issue:
30(8)
Published: Aug. 1, 2024
Soil
microbial
traits
and
functions
play
a
central
role
in
soil
organic
carbon
(SOC)
dynamics.
However,
at
the
macroscale
(regional
to
global)
it
is
still
unresolved
whether
(i)
specific
environmental
attributes
(e.g.,
climate,
geology,
types)
or
(ii)
community
composition
drive
key
directly.
To
address
this
knowledge
gap,
we
used
33
grassland
topsoils
(0-10
cm)
from
geoclimatic
gradient
Chile.
First,
incubated
soils
for
1
week
favorable
standardized
conditions
quantified
wide
range
of
such
as
biomass
(MBC),
enzyme
kinetics,
respiration,
growth
rates
well
use
efficiency
(CUE).
Second,
characterized
climatic
physicochemical
properties
bacterial
fungal
soils.
We
then
applied
regression
analysis
investigate
how
strongly
measured
were
linked
with
setting
versus
composition.
show
that
(predominantly
amount
matter)
determined
patterns
MBC
along
gradient,
which
turn
explained
respiration
rates.
normalized
(i.e.,
growth)
more
than
attributes.
Notably,
both
followed
distinct
trends
related
different
parts
community,
resulted
strong
effects
on
CUE.
conclude
even
macroscale,
CUE
result
physiologically
decoupled
aspects
metabolism,
partially
by
The
affect
functions,
therefore
factors
need
be
considered
context
SOC
Microbiology Spectrum,
Journal Year:
2024,
Volume and Issue:
12(7)
Published: May 17, 2024
ABSTRACT
Monkeypox
virus
(MPXV)
poses
a
global
health
threat.
Droplet
digital
PCR
(ddPCR)
holds
potential
as
an
accurate
diagnostic
tool
for
clinical
microbiology.
However,
there
is
limited
literature
on
the
applicability
of
ddPCR
in
settings.
In
this
study,
features
patients
with
MPXV
during
initial
outbreak
China
June
2023
were
reviewed,
and
optimized
method
dilution
and/or
inhibitor
removal
was
developed
to
enhance
detection
efficiency.
Eighty-two
samples
tested
from
nine
different
specimen
types,
including
feces,
urine,
pharyngeal
swabs,
anal
saliva,
herpes
fluid,
crust,
semen,
viral
load
each
quantified.
A
comparative
analysis
performed
qPCR
assess
sensitivity
specificity
investigate
characteristics
infection
by
analyzing
loads
specimens.
Consequently,
common
gastrointestinal
symptoms
observed
MPXV.
The
demonstrated
relatively
high
quantification
materials,
limit
0.1
copies/μL.
This
particularly
evident
low-concentration
like
whole
blood,
urine.
greater
accuracy
compared
normal
qPCR,
area
under
curve
(AUC)
0.939.
Except
crust
samples,
specimens
gradually
decreased
disease
progressed.
Virus
levels
feces
swabs
kept
rate
at
stage
post-symptom
onset,
may
be
suitable
diagnosis
continuous
monitoring
IMPORTANCE
technique
proved
sensitive
valuable
accurately
quantifying
various
types.
findings
provided
insights
into
necessary
pre-treatment
protocols
potentially
sample
types
collection.
Therefore,
such
results
can
aid
comprehending
usage
Viruses,
Journal Year:
2025,
Volume and Issue:
17(1), P. 86 - 86
Published: Jan. 11, 2025
Recent
decades
have
seen
growing
attention
on
viruses
in
the
environment
and
their
potential
impacts
as
a
result
of
global
epidemics.
Due
to
diversity
viral
species
along
with
complexity
environmental
host
factors,
virus
extraction
detection
methods
become
key
for
study
ecology.
This
review
systematically
summarises
extracting
detecting
pathogens
from
different
samples
(e.g.,
soil,
water,
faeces,
air)
biological
plants,
animals)
existing
studies,
comparing
similarities
differences,
applicability,
well
advantages
disadvantages
each
method.
Additionally,
this
discusses
future
directions
research
field.
The
aim
is
provide
theoretical
foundation
technical
reference
ecology
research,
facilitating
further
exploration
applications
