Myosin-5a facilitates stress granule formation by interacting with G3BP1 DOI Creative Commons
Rui Zhou, Jiabin Pan, Wenbo Zhang

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: Oct. 10, 2024

Stress granules (SGs) are non-membranous organelles composed of mRNA and proteins that assemble in the cytosol when cell is under stress. Although composition mammalian SGs both cell-type stress-dependent, they consistently contain core components, such as Ras GTPase activating protein SH3 domain binding 1 (G3BP1). Upon stress, living cells rapidly micrometric SGs, sometimes within a few minutes, suggesting SG components may be actively transported by microtubule and/or actin cytoskeleton. Indeed, assembly has been shown to depend on cytoskeleton associated motor proteins. However, role myosin remains controversial. Here, we identified G3BP1 novel unconventional myosin-5a (Myo5a). uses its C-terminal RNA-binding interact with middle portion Myo5a tail (Myo5a-MTD). Suppressing function cells, either overexpressing Myo5a-MTD, eliminating gene expression, or treatment myosin-5 inhibitor, inhibits arsenite-induced formation small large SGs. This different from effect disruption, which abolishes but enhances stress conditions. We therefore propose that, conditions, facilitates at an earlier stage than microtubule-dependent process.

Language: Английский

Population genetics reveal potential threats from low maternal genetic diversity in wild Asian elephants in China DOI Creative Commons
Minhui Shi, Yinping Tian,

Yongjing Tang

et al.

Global Ecology and Conservation, Journal Year: 2025, Volume and Issue: unknown, P. e03503 - e03503

Published: Feb. 1, 2025

Language: Английский

Citations

0
Natural resistance to cancers in long-lived mammals: genomic mechanisms and experimental evidence to explain Peto’s paradox DOI

Linxia Sun,

Zhi‐Kang Xu,

Mengqi Shuai

et al.

Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Language: Английский

Citations

0
Rapid evolution of genes with anti-cancer functions during the origins of large bodies and cancer resistance in elephants DOI Creative Commons

Jacob D. Bowman,

Vincent J. Lynch

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 29, 2024

Elephants have emerged as a model system to study the evolution of body size and cancer resistance because, despite their immense size, they very low prevalence cancer. Previous studies found that duplication tumor suppressors at least partly contributes anti-cancer cellular phenotypes in elephants. Still, many other mechanisms must contributed augmented resistance. Here, we use suite codon-based maximum-likelihood methods dataset 13,310 protein-coding gene alignments from 261

Language: Английский

Citations

2
Chromosome‐level Asian elephant genome assembly and comparative genomics of long‐lived mammals reveal the common substitutions for cancer resistance DOI Creative Commons
Xuanjing Li, Pengcheng Wang, Qi Pan

et al.

Aging Cell, Journal Year: 2023, Volume and Issue: 22(9)

Published: July 3, 2023

Abstract The naked mole rat ( Heterocephalus glaber ), bats (e.g., genus Myotis and elephants (family Elephantidae) are known as long‐lived mammals assumed to be excellent cancer antagonists. However, whether there common genetic changes underpinning resistance in these species is yet fully established. Here, we newly generated a high‐quality chromosome‐level Asian elephant Elephas maximus ) genome identified that the expanded gene families involved Ras‐associated base excision repair pathways. Moreover, performed comparative genomic analyses of 12 examined genes with signatures positive selection elephants, rat, greater horseshoe bat. Residues at positively selected sites CDR2L ALDH6A1 enhanced inhibition tumor cell migration compared those short‐lived relatives. Overall, our study provides new resource preliminary survey mammals.

Language: Английский

Citations

5
Myosin-5a facilitates stress granule formation by interacting with G3BP1 DOI Creative Commons
Rui Zhou, Jiabin Pan, Wenbo Zhang

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: Oct. 10, 2024

Stress granules (SGs) are non-membranous organelles composed of mRNA and proteins that assemble in the cytosol when cell is under stress. Although composition mammalian SGs both cell-type stress-dependent, they consistently contain core components, such as Ras GTPase activating protein SH3 domain binding 1 (G3BP1). Upon stress, living cells rapidly micrometric SGs, sometimes within a few minutes, suggesting SG components may be actively transported by microtubule and/or actin cytoskeleton. Indeed, assembly has been shown to depend on cytoskeleton associated motor proteins. However, role myosin remains controversial. Here, we identified G3BP1 novel unconventional myosin-5a (Myo5a). uses its C-terminal RNA-binding interact with middle portion Myo5a tail (Myo5a-MTD). Suppressing function cells, either overexpressing Myo5a-MTD, eliminating gene expression, or treatment myosin-5 inhibitor, inhibits arsenite-induced formation small large SGs. This different from effect disruption, which abolishes but enhances stress conditions. We therefore propose that, conditions, facilitates at an earlier stage than microtubule-dependent process.

Language: Английский

Citations

1