CD44 Modulates Cell Migration and Invasion in Ewing Sarcoma Cells DOI Open Access
Enrique Fernández-Tabanera, Laura García‐García, Carlos Rodríguez-Martín

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(14), P. 11774 - 11774

Published: July 21, 2023

The chimeric EWSR1::FLI1 transcription factor is the main oncogenic event in Ewing sarcoma. Recently, it has been proposed that levels can fluctuate sarcoma cells, giving rise to two cell populations. EWSR1::FLI1low cells present a migratory and invasive phenotype, while EWSR1::FLI1high are more proliferative. In this work, we described how CD44 standard isoform (CD44s), transmembrane protein involved adhesion migration, overexpressed phenotype. functional characterization of CD44s (proliferation, clonogenicity, invasion ability) was performed three doxycycline-inducible models (A673, MHH-ES1, CADO-ES1). As result, expression reduced proliferation all lines tested without affecting clonogenicity. Additionally, increased migration A673 effects CADO-ES1. hyaluronan ligand CD44s, its effect on ability also assessed, showing high molecular weight hyaluronic acid (HMW-HA) blocked low (LMW-HA) it. Invasion correlated with MHH-ES1 lines. upregulated upon knockdown, regulates cells.

Language: Английский

Development of Quercetin Solid Dispersion-Loaded Dissolving Microneedles and In Vitro Investigation of Their Anti-Melanoma Activities DOI Creative Commons

Monsicha Khuanekkaphan,

Kesinee Netsomboon,

Adryan Fristiohady

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(10), P. 1276 - 1276

Published: Sept. 30, 2024

Melanoma is a skin cancer that requires early treatment to prevent metastasis. In particular, the superficial spreading melanoma, excisional surgery with local administration of anti-cancer drugs via microneedles currently considered potential combination therapy. Quercetin natural flavonoid having activities against melanoma cells. Unfortunately, therapeutic effect limited by its poor water solubility.

Language: Английский

Citations

4
Synergistic enhancement of low-dose radiation therapy via cuproptosis and metabolic reprogramming for radiosensitization in in situ hepatocellular carcinoma DOI Creative Commons

Ni Shao,

Yongqing Yang,

Genwen Hu

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 19, 2024

Radiotherapy (RT) is a primary clinical approach for cancer treatment, but its efficacy often hindered by various challenges, especially radiation resistance, which greatly compromises the therapeutic effectiveness of RT. Mitochondria, central to cellular energy metabolism and regulation cell death, play critical role in mechanisms radioresistance. In this context, cuproptosis, novel copper-induced mitochondria-respiratory-dependent death pathway, offers promising avenue radiosensitization. study, an innovative theranostic nanoplatform was designed induce cuproptosis synergy with low-dose therapy (LDRT, i.e., 0.5–2 Gy) treatment situ hepatocellular carcinoma (HCC). This aims reverse hypoxic tumor microenvironment, promoting shift from glycolysis oxidative phosphorylation (OXPHOS), thereby enhancing sensitivity cuproptosis. Concurrently, Fenton-like reaction ensures sustained supply copper depletion glutathione (GSH), inducing disrupting mitochondrial function, interrupting supply. strategy effectively overcomes radioresistance enhances against tumors. conclusion, study elucidates intricate interactions among hypoxia reversal, metabolic reprogramming, radiosensitization, particularly context treating carcinoma, providing paradigm radiotherapy.

Language: Английский

Citations

4
Synthesis, characterization, and in vitro anti-tumor activity studies of the hyaluronic acid-mangiferin-methotrexate nanodrug targeted delivery system DOI
Haojue Wang,

Wanfei Shao,

Xianyi Lü

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 239, P. 124208 - 124208

Published: March 25, 2023

Language: Английский

Citations

10
CD44 in Bone Metastasis Development: A Key Player in the Fate Decisions of the Invading Cells? DOI

Noy Shir Zer,

Noa Ben-Ghedalia-Peled, Levi A. Gheber

et al.

Clinical & Experimental Metastasis, Journal Year: 2023, Volume and Issue: 40(2), P. 125 - 135

Published: April 1, 2023

Language: Английский

Citations

10
CD44 Modulates Cell Migration and Invasion in Ewing Sarcoma Cells DOI Open Access
Enrique Fernández-Tabanera, Laura García‐García, Carlos Rodríguez-Martín

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(14), P. 11774 - 11774

Published: July 21, 2023

The chimeric EWSR1::FLI1 transcription factor is the main oncogenic event in Ewing sarcoma. Recently, it has been proposed that levels can fluctuate sarcoma cells, giving rise to two cell populations. EWSR1::FLI1low cells present a migratory and invasive phenotype, while EWSR1::FLI1high are more proliferative. In this work, we described how CD44 standard isoform (CD44s), transmembrane protein involved adhesion migration, overexpressed phenotype. functional characterization of CD44s (proliferation, clonogenicity, invasion ability) was performed three doxycycline-inducible models (A673, MHH-ES1, CADO-ES1). As result, expression reduced proliferation all lines tested without affecting clonogenicity. Additionally, increased migration A673 effects CADO-ES1. hyaluronan ligand CD44s, its effect on ability also assessed, showing high molecular weight hyaluronic acid (HMW-HA) blocked low (LMW-HA) it. Invasion correlated with MHH-ES1 lines. upregulated upon knockdown, regulates cells.

Language: Английский

Citations

10