Differential regulation of heparan sulfate biosynthesis in fibroblasts cocultured with normal vs. cancerous prostate cells DOI Creative Commons
Elvira V. Grigorieva, Anastasia V. Strokotova, Ingemar Ernberg

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 9, 2024

Heparan sulfate proteoglycans (HSPGs) regulate a wide range of biological activities in both physiological and pathological conditions. Altered expression or deregulated function HSPGs their heparan (HS) chains significantly contribute to carcinogenesis as well crucially depends on the functioning complex system HS biosynthetic/modifying enzymes termed “GAGosome”. Here, we aimed at investigating profile cell culture model stroma-epithelial crosstalk searching for transcription factors potentially related regulation genes involved. Coculture BjTERT-fibroblasts with normal PNT2 human prostate epithelial cells resulted significant downregulation (2-4-fold) transcriptional activity metabolism-involved ( EXT1/2, NDST1/2, GLCE, HS2ST1, HS3ST1/2, HS6ST1/2, SULF1/2, HPSE ) types, whereas coculture cancer (LNCaP, PC3, DU145) demonstrated no interchanges. Human Transcription Factor RT 2 Profiler PCR array manual RT-PCR verification supposed FOS, MYC, E2F, SRF, NR3C1 potential candidates and/or coordination biosynthesis. Taken together, biosynthetic fibroblasts during might be controlled by intercellular communication, reflecting adaptation these each other. The is attenuated abrogated if interact making independent limiting effects fibroblasts, thus contributing possibility unlimited growth progression. Overall, data demonstrate an ability cell-cell interactions affect biosynthesis-involved genes.

Language: Английский

Mechanisms of assembly and remodelling of the extracellular matrix DOI
Alexandra Naba

Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 2, 2024

Language: Английский

Citations

42
Extracellular Matrix Components and Mechanosensing Pathways in Health and Disease DOI Creative Commons
Aikaterini Berdiaki, Monica Neagu, Petros Tzanakakis

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1186 - 1186

Published: Sept. 20, 2024

Glycosaminoglycans (GAGs) and proteoglycans (PGs) are essential components of the extracellular matrix (ECM) with pivotal roles in cellular mechanosensing pathways. GAGs, such as heparan sulfate (HS) chondroitin (CS), interact various cell surface receptors, including integrins receptor tyrosine kinases, to modulate responses mechanical stimuli. PGs, comprising a core protein covalently attached GAG chains, serve dynamic regulators tissue mechanics behavior, thereby playing crucial role maintaining homeostasis. Dysregulation GAG/PG-mediated pathways is implicated numerous pathological conditions, cancer inflammation. Understanding intricate mechanisms by which GAGs PGs forces holds promise for developing novel therapeutic strategies targeting mechanotransduction disease. This comprehensive overview underscores importance key mediators homeostasis their potential targets mitigating mechano-driven pathologies, focusing on

Language: Английский

Citations

12
Regulation of Joint Tissues and Joint Function: Is There Potential for Lessons to Be Learned Regarding Regulatory Control from Joint Hypermobility Syndromes? DOI Open Access
David A. Hart

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1256 - 1256

Published: Jan. 31, 2025

Normal development of joints starts in utero with the establishment a cellular and extracellular matrix template. Following birth, individual joint tissues grow mature response to biochemical mechanical signals, leading coordinated pattern further maturation resulting that functions as an organ system. Each develops matures system defined by biomechanical environment which it will function. For those hypermobility syndromes, either specific genetic mutations or not (i.e., Ehlers–Danlos syndrome, Marfan Loey–Dietz hypermobility-type syndrome), this process is partially compromised, but many aspects tissue function retained such organs form retain partial function, compromised. Comparing characteristics what known regarding development, growth, maturation, stressors puberty, pregnancy, aging without leads conclusion have systems may be compromised via failure undergo possibly defective mechanotransduction. Given breadth involved characterization concept reveal commonalities their impact on inform regulatory normal functional integrity. This review/perspective piece attempt detail comparisons summarize how study aid understanding.

Language: Английский

Citations

1
Genetic Deficiencies of Hyaluronan Degradation DOI Creative Commons
Stephen P. Fink, Barbara L. Triggs‐Raine

Cells, Journal Year: 2024, Volume and Issue: 13(14), P. 1203 - 1203

Published: July 16, 2024

Hyaluronan (HA) is a large polysaccharide that broadly distributed and highly abundant in the soft connective tissues embryos of vertebrates. The constitutive turnover HA very high, estimated at 5 g per day an average (70 kg) adult human, but must also be tightly regulated some processes. Six genes encoding homologues to bee venom hyaluronidase (

Language: Английский

Citations

5
New insights into polysaccharide-based nanostructured delivery systems in breast cancer: Possible application of antisense oligonucleotides in breast cancer therapy DOI

Fahimeh Shayegh,

Zeynep Türk, Arta Armani

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 272, P. 132890 - 132890

Published: June 1, 2024

Language: Английский

Citations

4
A year in the molecular life sciences: 2024 highlights DOI Creative Commons
Julija Hmeljak, Hajrah Khawaja, Séamus J. Martin

et al.

FEBS Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

The FEBS Journal editorial team reviews the articles we published in 2024 and reflects on year's highlights. summarised here broadly cluster three themes—molecular cell biology across species, immunology, cutting‐edge methods—whilst still showcasing diversity of scientific fields journal covers. We look forward to many more excellent 2025 hope these highlights will inspire you submit your next manuscript Journal.

Language: Английский

Citations

0
Comprehensive Investigation of Proteoglycan Gene Expression in Breast Cancer: Discovery of a Unique Proteoglycan Gene Signature Linked to the Malignant Phenotype DOI Creative Commons
Simone Buraschi, Gabriel J. Pascal, Federico Liberatore

et al.

Proteoglycan Research, Journal Year: 2025, Volume and Issue: 3(1)

Published: Jan. 1, 2025

Solid tumors present a formidable challenge in oncology, necessitating innovative approaches to improve therapeutic outcomes. Proteoglycans, multifaceted molecules within the tumor microenvironment, have garnered attention due their diverse roles cancer progression. Their unique ability interact with specific membrane receptors, growth factors, and cytokines provides promising avenue for development of recombinant proteoglycan-based therapies that could enhance precision efficacy treatment. In this study, we performed comprehensive analysis proteoglycan gene landscape human breast carcinomas. Leveraging available wealth genomic clinical data regarding expression carcinoma using machine learning model, identified signature composed five proteoglycans differentially modulated tissue: Syndecan-1 asporin (upregulated) decorin, PRELP podocan (downregulated). Additional query revealed serglycin, previously shown be increased patients mouse models correlate poor prognosis, was indeed decreased vast majority its levels inversely correlated progression invasion. This provide novel diagnostic capabilities biology highlights need further utilization publicly datasets validation preclinical experimental results.

Language: Английский

Citations

0
Sulfated Glycosaminoglycans as Inhibitors for Chlamydia Infections: Molecular Weight and Sulfation Dependence DOI Creative Commons

Sebastian Wintgens,

Janita Müller,

Felicitas Drees

et al.

Macromolecular Bioscience, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Abstract Glycosaminoglycans (GAGs) play a pivotal role in pathogen attachment and entry into host cells, where the interaction with GAGs is critical for diverse range of bacteria viruses. This study focuses on elucidating specific interactions between sulfated adhesin OmcB (Outer membrane complex protein B) Chlamydia species, examining how structural characteristics GAGs, such as sulfation degree molecular weight, influence their binding affinity thereby affect bacterial infectivity. A surface‐based assay established to determine constants various GAGs. It shown that increased higher weight enhance GAG OmcB. These findings are further validated using cell assays, which shows addition reduces OmcB‐cell inhibits C. pneumoniae elementary bodies (EBs), underscoring chlamydial infections. Notably, heparin exhibites stronger inhibitory effect compare similar degrees weights, suggesting particular architectures may optimize interactions.

Language: Английский

Citations

0
Therapeutic targets in aging-related osteoarthritis: A focus on the extracellular matrix homeostasis DOI Creative Commons
Hao Wan, Ming‐Fu Chang, Di Shi

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123487 - 123487

Published: Feb. 1, 2025

Language: Английский

Citations

0
The interplay between endothelial glycocalyx maturity and both the toxicity and intracellular uptake of charged nanoparticles DOI Creative Commons
Claire Bridges, Lu Fu, Jonathan Yeow

et al.

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0