Molecular and Cellular Biochemistry, Journal Year: 2022, Volume and Issue: 478(5), P. 1045 - 1057
Published: Oct. 11, 2022
Language: Английский
Medical Oncology, Journal Year: 2025, Volume and Issue: 42(5)
Published: March 31, 2025
Language: Английский
Citations
2
Frontiers in Oncology, Journal Year: 2022, Volume and Issue: 12
Published: March 7, 2022
A number of studies have demonstrated that circular RNA (circRNA) plays a critical role in tumorigenesis and tumor progression. However, the biological effects most circRNAs on cervical cancer remain unclear. Hsa_circ_0021087 (thereafter named circLMO1) is circRNA generated from circularization exon 2 3 LIM Domain Only 1 (LMO1) first identified as suppressor gastric cancer. We aimed to identify circLMO1 progression.CircLMO1 was verified through qPCR Sanger sequencing. The regulating growth metastasis investigated both vitro nude mouse xenograft model. dual luciferase reporter assay rescue experiment were conducted evaluate interactions among circLMO1, microRNA (miR)-4291, acyl-CoA synthetase long chain family member 4 (ACSL4). ferroptosis assessed by analyzing lipid reactive oxygen species (ROS), malonyl dialdehyde (MDA), glutathione (GSH) content.The level down-regulated tissues associated with International Federation Gynecology Obstetrics (FIGO) staging. Functionally, overexpression inhibited vivo, whereas depletion promoted cell proliferation invasion. Mechanistically, acted competing endogenous (ceRNA) sponging miR-4192 repress target gene ACSL4. CircLMO1 up-regulating ACSL4 expression. Overexpression miR-4291 or knockdown reversed effect facilitating repressing invasion.CircLMO1 miR-4291/ACSL4-mediated ferroptosis, could be promising biomarker for clinical management
Language: Английский
Citations
63
Journal of Cellular and Molecular Medicine, Journal Year: 2021, Volume and Issue: 25(8), P. 3667 - 3679
Published: March 9, 2021
Circular RNA (circRNA) is a highly abundant type of single-stranded non-coding RNA. Novel research has discovered many roles circRNA in colorectal cancer (CRC) including proliferation, metastasis and apoptosis. Furthermore, circRNAs also play role the development drug resistance have unique associations with tumour size, staging overall survival CRC that lend potential to serve as diagnostic prognostic biomarkers. Among cancers worldwide, ranks second mortality third incidence. In order better understanding influence on progression, this review summarizes specific evaluates their value therapeutic targets biomarkers for CRC. We aim provide insight therapy clinical decision-making.
Language: Английский
Citations
60
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4548 - 4548
Published: April 21, 2024
Colorectal cancer (CRC) is the third most prevalent to be diagnosed, and it has a substantial mortality rate. Despite numerous studies being conducted on CRC, remains significant health concern. The disease-free survival rates notably decrease as CRC progresses, emphasizing urgency for effective diagnostic therapeutic approaches. development caused by environmental factors, which mostly lead disruption of signaling pathways. Among these pathways, Wingless/Integrated (Wnt) pathway, Phosphatidylinositol 3-kinase/protein kinase B/mammalian target rapamycin (PI3K/AKT/mTOR) Mitogen-Activated Protein Kinase (MAPK) Transforming Growth Factor-β (TGF-β) p53 pathway are considered important. These pathways also regulated non-coding RNAs (ncRNAs), including microRNAs (miRNAs), long (lncRNAs), circular (circRNAs). They have emerged crucial regulators gene expression in changing their levels. altered patterns ncRNAs been implicated progression development, suggesting potential targets. This review provides an overview five key regulation involved pathogenesis that studied identify promising avenues diagnosis treatment strategies.
Language: Английский
Citations
12
Wiley Interdisciplinary Reviews - RNA, Journal Year: 2024, Volume and Issue: 15(3)
Published: May 1, 2024
Abstract Circular RNAs (circRNAs), characterized by their closed‐loop structure, have emerged as significant transcriptomic regulators, with roles spanning from microRNA sponging to modulation of gene expression and potential peptide coding. The discovery functional analysis circRNAs been propelled advancements in both experimental bioinformatics tools, yet the field grapples challenges related detection, isoform diversity, accurate quantification. This review navigates through evolution circRNA research methodologies, early detection techniques current state‐of‐the‐art approaches that offer comprehensive insights into biology. We examine limitations existing methods, particularly difficulty differentiating isoforms distinguishing linear counterparts. A critical evaluation various tools novel strategies is presented, emphasizing need for integrated enhance our understanding interpretation functions. Our underscore dynamic rapidly advancing nature research, highlighting ongoing development analytical frameworks designed address complexity facilitate assessment clinical utility. As such, this overview aims catalyze further study, fostering a deeper cellular processes implications disease. article categorized under: RNA Methods > Nanotechnology Analyses Cells In Vitro Silico
Language: Английский
Citations
9
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5699 - 5699
Published: May 23, 2024
Ulcerative colitis (UC), an inflammatory bowel disease (IBD), may increase the risk of colorectal cancer (CRC) by activating chronic proinflammatory pathways. The goal this study was to find serum prediction biomarkers in UC CRC development combining low-density miRNA microarray and biocomputational approaches. expression profiles were compared microarray, finding five upregulated miRNAs specific progression (hsa-let-7d-5p, hsa-miR-16-5p, hsa-miR-145-5p, hsa-miR-223-5p, hsa-miR-331-3p). circRNA/miRNA/mRNA competitive endogenous RNA (ceRNA) network analysis showed that candidate connected well-known colitis-associated ACVR2A, SOCS1, IGF2BP1, FAM126A, CCDC85C mRNAs, circ-SHPRH circRNA. SST SCARA5 genes regulated hsa-let-7d-5p, hsa-miR-331-3p linked a poor survival prognosis patient dataset from Cancer Genome Atlas (TCGA). Lastly, our mRNA candidates validated comparing their differentially expressed mRNAs tissue databases. A high level parallel reduction SOCS1 found serum. We propose possibly hsa-let-7d-5p as novel for predicting CRC. More clinical sample is required further validation.
Language: Английский
Citations
7
Biomolecules, Journal Year: 2021, Volume and Issue: 11(12), P. 1755 - 1755
Published: Nov. 24, 2021
Circular RNA (circRNA) is a distinct class of non-coding produced, in principle, using back-splicing mechanism, conserved during evolution, with increased stability and tissue-dependent expression. represents functional molecule roles the regulation transcription splicing, microRNA sponge, modulation protein–protein interaction. CircRNAs are involved essential processes life such as apoptosis, cell cycle, proliferation. Due to regulatory role (upregulation/downregulation) pathogenic mechanisms some diseases (including cancer), its potential biomarker or therapeutic target these were studied. This review focuses on importance circular cancer.
Language: Английский
Citations
39
Cell Death and Disease, Journal Year: 2022, Volume and Issue: 13(11)
Published: Nov. 29, 2022
Abstract Colorectal cancer (CRC) is the third most frequently diagnosed with unfavorable clinical outcomes worldwide. circFNDC3B plays as a tumor suppressor in CRC, however, mechanism of CRC remains ambiguous. The stem-like properties cells were detected by evaluation stemness markers, sphere formation assay and flow cytometry. qRT-PCR, FISH, IHC, western blotting assessed expression localization circFNDC3B, RNF41, ASB6, markers CRC. metastatic capabilities examined wound healing Transwell assays, well vivo liver metastasis model. Bioinformatics analysis, RNA immunoprecipitation (RIP), pull-down co-IP used to detect associations among FXR2, ASB6. Downregulated was associated unfavorite survival patients, overexpression suppressed metastasis. Mechanistically, studies revealed that YTHDC1 facilitated cytoplasmic translocation m 6 A-modified enhanced RNF41 mRNA stability via binding FXR2. promoted ASB6 degradation through RNF41-mediated ubiquitination. Functional showed silencing counteracted circFNDC3B-suppressed metastasis, reversed circFNDC3B- or regulation Elevated positively correlated patients. In experiments further repressed growth, modulating Taken together, inhibited RNF41-dependent degradation. These findings provide novel insights important clues for targeted therapeutic strategies
Language: Английский
Citations
27
Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 255, P. 155216 - 155216
Published: Feb. 16, 2024
Language: Английский
Citations
5
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11535 - 11535
Published: Oct. 27, 2024
Biomarkers in colorectal cancer (CRC) are of great interest the current literature due to improvements techniques such as liquid biopsy and next-generation sequencing (NGS). However, screening methods vary globally, with multi-target stool DNA (mt-sDNA) predominantly used USA and, more recently, Cologuard Plus; biomarkers Galectins family septins show promise early detection. Gut microbiome assessments, Fusobacterium nucleatum, under intense exploration. Diagnostic tests, circulating analysis via NGS, exhibit effectiveness being increasingly adopted. Circulating tumor cells emerge potential alternatives traditional terms diagnosis prognosis. Predictive well established guidelines; nonetheless, aid machine learning artificial intelligence, these may be improved. This review critically explores actual dynamic landscape CRC future, promising involved screening, diagnosis,
Language: Английский
Citations
5