RNAi as a Foliar Spray: Efficiency and Challenges to Field Applications

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(12), P. 6639 - 6639

Published: June 14, 2022

RNA interference (RNAi) is a powerful tool that being increasingly utilized for crop protection against viruses, fungal pathogens, and insect pests. The non-transgenic approach of spray-induced gene silencing (SIGS), which relies on spray application double-stranded (dsRNA) to induce RNAi, has come prominence due its safety environmental benefits in addition wide host range high target specificity. However, along with promising results recent studies, several factors limiting SIGS RNAi efficiency have been recognized insects plants. While sprayed dsRNA the plant surface can produce robust response some chewing insects, uptake systemic movement required delivery many other organisms. For example, pests such as sucking require presence vascular tissues, while pathogens are predominately located internal tissues. Investigating mechanisms by enters moves through tissues understanding barriers may hinder this process essential developing efficient ways deliver into systems. In review, we assess current knowledge foliar cellular molecules. We will also identify major uptake, including leaf morphological features well factors, address methods overcome these barriers.

Language: Английский

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Can biocontrol be the game-changer in integrated pest management? A review of definitions, methods and strategies

Journal of Plant Diseases and Protection, Journal Year: 2024, Volume and Issue: 131(2), P. 265 - 291

Published: Feb. 23, 2024

Abstract Global agriculture is heavily dependent on sustainable plant protection. Worldwide, the concept of integrated pest management (IPM) being followed. IPM utilizes a range strategies, with chemical synthetic pesticides employed only as last resort. However, in agricultural practice, farmers continue to rely primarily this option. To further reduce dependence, new strategies are sought strengthen use biological control within approach including identification novel non-synthetic natural compounds. Here, we discuss and report state art research areas such biocontrol agents application ecological principles. These practices can help establish protection systems, greatest impact achieved when they used appropriate combinations. We highlight conditions that currently prevent or hinder increased measures. On background agroecological experiences, why additional advancements imperative more effectively break life cycles pests, diseases weeds. emphasize significance judicious technologies, adapted local conditions. Additionally, key role expertise operators implementing these their knowledge thereof.

Language: Английский

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RNAi Crop Protection Advances

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(22), P. 12148 - 12148

Published: Nov. 10, 2021

RNAi technology is a versatile, effective, safe, and eco-friendly alternative for crop protection. There plenty of evidence its use through host-induced gene silencing (HIGS) emerging that spray-induced (SIGS) techniques can work as well to control viruses, bacteria, fungi, insects, nematodes. For SIGS, most significant challenge achieving stability avoiding premature degradation in the environment or during absorption by target organism. One encapsulation liposomes, virus-like particles, polyplex nanoparticles, bioclay, which be obtained recombinant production vectors, transgenesis, micro/nanoencapsulation. The materials must biodegradable, stable multiple chemical environments, favoring controlled release RNAi. Most current research on encapsulated focuses primarily oral delivery insects essential genes. regulation risk assessment using different approaches; however, this has positive economic, environmental, human health implications agriculture. emergence alternatives combining with induction resistance crops elicitation metabolic expected, biotechnological optimization large-scale production.

Language: Английский

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Nanoparticle-Shielded dsRNA Delivery for Enhancing RNAi Efficiency in Cotton Spotted Bollworm Earias vittella (Lepidoptera: Nolidae)

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9161 - 9161

Published: May 23, 2023

The spotted bollworm Earias vittella (Lepidoptera: Nolidae) is a polyphagous pest with enormous economic significance, primarily affecting cotton and okra. However, the lack of gene sequence information on this has significant constraint molecular investigations formulation superior management strategies. An RNA-seq-based transcriptome study was conducted to alleviate such limitations, de novo assembly performed obtain transcript sequences pest. Reference identification across E. developmental stages RNAi treatments were using its information, which resulted in identifying transcription elongation factor (TEF), V-type proton ATPase (V-ATPase), Glyceraldehyde -3-phosphate dehydrogenase (GAPDH) as most suitable reference genes for normalization RT-qPCR-based expression studies. present also identified important developmental, pathway, target life-stage analysis RT-qPCR select optimal targets RNAi. We found that naked dsRNA degradation hemolymph primary reason poor A total six including Juvenile hormone methyl transferase (JHAMT), Chitin synthase (CHS), Aminopeptidase (AMN), Cadherin (CAD), Alpha-amylase (AMY), (V-ATPase) selected knocked down significantly three different nanoparticles encapsulated conjugates, i.e., Chitosan-dsRNA, carbon quantum dots-dsRNA (CQD-dsRNA), Lipofectamine-dsRNA conjugate. These results demonstrate feeding nanoparticle-shielded silences suggests nanoparticle-based can efficiently manage

Language: Английский

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Nanoparticle LDH enhances RNAi efficiency of dsRNA in piercing-sucking pests by promoting dsRNA stability and transport in plants

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 6, 2024

Language: Английский

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Oligonucleotide insecticides (contact unmodified antisense DNA biotechnology) and RNA biocontrols (double-stranded RNA technology): newly born fraternal twins in plant protection

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 14, 2024

Abstract The rapid development of molecular genetics, methods synthesis nucleic acids, and bioinformatics have created fundamentally new opportunities in plant protection. DNA RNA become active ingredients insecticides, going beyond their usual role as carriers hereditary information. Now acids are increasingly acting tools for insect pest control using the mechanisms containment (oligonucleotide contact unmodified antisense (CUAD) biotechnology) interference (RNA biocontrols, double-stranded biotechnology). Partial understanding how oligonucleotides might work began late 1970s XX century a long time (until beginning XXI century) these biomolecules had not been implemented protection form any biotechnology based on or modified fragments. was discovered 90s also went through quite path before first encouraging results its application insecticides appeared. Both, CUAD technology aim to inactivate expression genes pests that help trigger degradation target RNA. As two classes next-generation products supposed operational life, low carbon footprint, high selectivity action biodegradation ecosystems. To date, shows best sap-sucking insects while insecticidal potential beetles from Tenebrionidae Chrysomelidae families. With probability, oligonucleotide will be effective against certain groups can complement each other complex formulations wide range pests.

Language: Английский

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Host-Delivered RNA Interference for Durable Pest Resistance in Plants: Advanced Methods, Challenges, and Applications

Molecular Biotechnology, Journal Year: 2023, Volume and Issue: 66(8), P. 1786 - 1805

Published: July 31, 2023

Language: Английский

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Control of two insect pests by expression of a mismatch corrected double‐stranded RNA in plants

Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: 22(7), P. 2010 - 2019

Published: March 1, 2024

Summary RNA interference (RNAi) has emerged as an efficient technology for pest control by silencing the essential genes of targeted insects. Owing to its nucleotide sequence‐guided working mechanism, RNAi a high degree species‐specificity without impacts on non‐target organisms. However, plants are inevitably under threat two or more insect pests in nature, species‐specific mode RNAi‐based restricts wide application control. In this study, we artificially designed intermediate dsRNA ( iACT ) targeting β‐Actin ACT sap‐sucking Bemisia tabaci and Myzus persicae mutual correction their mismatches. When expressing hairpin (hp from tobacco nuclear genome, transgenic well protected both B. M. , either individually simultaneously, evidenced reduced fecundity suppressed gene expression, whereas expression hpRNA BtACT MpACT could only confer specific resistance respectively. sum, our data provide novel proof‐of‐concept that different species be simultaneously controlled artificial synthesis with sequence optimization, which expands range methods crop protection.

Language: Английский

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Nanoscale Metal–Organic Frameworks for the Co-Delivery of Cycloxaprid and Pooled siRNAs to Enhance Control Efficacy in Diaphorina citri

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(6), P. 3353 - 3362

Published: Jan. 31, 2025

RNA pesticides have emerged as a promising alternative to conventional chemical due their high specificity and minimal environmental impact. However, the instability of molecules in environment challenges associated with effective delivery target pests limit broader application. This study addresses these by developing dual system using chitosan (CS) Metal-Organic Frameworks (MOFs) enhance efficacy double-stranded (dsRNA) cycloxaprid against Diaphorina citri, vector citrus greening disease. The CS-MOF nanoparticles were synthesized characterized scanning electron microscopy (SEM) dynamic light scattering (DLS). Insect bioassays demonstrated that codelivery significantly improved insecticidal activity, achieving over 80% mortality D. citri within 2 days. results indicate encapsulation dsRNA MOFs enhances its stability, while controlled release properties improve cycloxaprid. novel approach shows great potential overcoming limitations offers sustainable solution for pest management agriculture. Future research should optimize system, conduct field trials, explore applicability other agricultural pests.

Language: Английский

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Harnessing double-stranded RNA (dsRNA): a sustainable approach to pest management

Physiology and Molecular Biology of Plants, Journal Year: 2025, Volume and Issue: unknown

Published: March 2, 2025

Language: Английский

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