Inflammation alters the expression and activity of the mechanosensitive ion channels in periodontal ligament cells DOI

Jianhan Ren,

Chaoning Zhan, Yifan Lin

et al.

European Journal of Orthodontics, Journal Year: 2024, Volume and Issue: 47(1)

Published: Dec. 4, 2024

Abstract Background Periodontal ligament cells (PDLCs) possess mechanotransduction capability, vital in orthodontic tooth movement (OTM) and maintaining periodontal homeostasis. The study aims to elucidate the expression profiles of mechanosensitive ion channel (MIC) families PDLCs how inflammatory mediator alters their function, advancing understanding biological process OTM. Methods methods Human were cultured exposed TNF-α. RNA sequencing was conducted explore mRNA transcriptome both normal TNF-α-treated PDLCs. Differentially expressed MICs identified analyzed. functional expressions TRPA1 TRPM8 further validated by RT-qPCR, Western blot, calcium influx assays. Results All 10 MIC or subfamilies PDLCs, with TRP family being most abundant. KCNK2, PIEZO1, TMEM87A, PKD2 channels TNF-α altered families, resulting increased PIEZO, K2P, TRP, TMEM63, TMEM87 decreased ENaC/ASIC, TMC/TMHS/TMIE, TMEM150, TMEM120, L/T/N-Type families. Furthermore, 17 DEMICs (false discovery rate < 0.05), top five (fold change ≥ 2), including upregulated TRPM8. verified, suggesting that significantly sensitized activities. Conclusions provides comprehensive reveals inflammation activities MICs. Treatments targeting these may offer promising strategies for improving OTM preventing complications environments, ultimately leading more effective safer practices.

Language: Английский

Exploring the mechanical and biological interplay in the periodontal ligament DOI Creative Commons
Xinyu Wen, Fang Pei, Ying Jin

et al.

International Journal of Oral Science, Journal Year: 2025, Volume and Issue: 17(1)

Published: April 2, 2025

Language: Английский

Citations

1
Nociceptor mechanisms underlying pain and bone remodeling via orthodontic forces: toward no pain, big gain DOI Creative Commons
Sheng Wang, Ching‐Chang Ko, Man‐Kyo Chung

et al.

Frontiers in Pain Research, Journal Year: 2024, Volume and Issue: 5

Published: Feb. 22, 2024

Orthodontic forces are strongly associated with pain, the primary complaint among patients wearing orthodontic braces. Compared to other side effects of treatment, pain is often overlooked, limited clinical management. lead inflammatory responses in periodontium, which triggers bone remodeling and eventually induces tooth movement. Mechanical subsequent inflammation periodontium activate sensitize periodontal nociceptors produce pain. Nociceptive afferents expressing transient receptor potential vanilloid subtype 1 (TRPV1) play central roles transducing nociceptive signals, leading transcriptional changes trigeminal ganglia. molecules, such as TRPV1, ankyrin 1, acid-sensing ion channel 3, P2X3 receptor, believed mediate Neuropeptides calcitonin gene-related peptides substance P can also regulate While transmit signals brain, they known modulate alveolar periodontitis. Therefore, molecules may contribute modulation movement, currently remains undetermined. Future studies needed better understand fundamental mechanisms underlying neuroskeletal interactions orthodontics improve treatment by developing novel methods reduce accelerate movement—thereby achieving “big gains no pain” orthodontics.

Language: Английский

Citations

8
Piezo1 promotes double-directional differentiation from human periodontal ligament progenitor cells DOI

Yuri Kono,

Hiroshi Kajiya, R Nagano

et al.

Journal of Oral Biosciences, Journal Year: 2025, Volume and Issue: unknown, P. 100651 - 100651

Published: March 1, 2025

Language: Английский

Citations

0
Utilizing vibration as a method for accelerating tooth movement during orthodontic treatment: a systematic review and meta-analysis DOI Creative Commons

Shubhobrata Dutta,

Amol Patil,

Sonakshi Sharma

et al.

Clinical and Investigative Orthodontics, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 16

Published: April 2, 2025

Language: Английский

Citations

0
Febrile temperature-regulated TRPV1 in CD4+ T cells mediates neuroinflammation in complex febrile seizures DOI Creative Commons

Shuo Kong,

Xianglei Jia,

Liang Xin

et al.

Journal of Neuroinflammation, Journal Year: 2025, Volume and Issue: 22(1)

Published: April 7, 2025

Language: Английский

Citations

0
PIEZO1 Promotes Odontoblast-Mediated Reactionary Dentinogenesis via SEMA3A DOI
Pin‐I Huang,

R.X. Jiang,

Fei Wang

et al.

Journal of Dental Research, Journal Year: 2024, Volume and Issue: 103(9), P. 889 - 898

Published: June 24, 2024

Located at the interface of dentin-pulp complex, odontoblasts are specialized cells responsible for dentin synthesis and nociceptive signal detection in response to external stimuli. Recent studies have shown that mechanosensitive ion channel PIEZO1 is involved bone formation remodeling through influx calcium ions, it abundantly expressed odontoblasts. However, specific role reactionary dentinogenesis underlying mechanisms remain elusive. In this study, we found intense expression plasma membrane cytoplasm healthy human third molars, mouse mandibular odontoblast-like (hOBLCs). hOBLCs, positively regulated DSPP, DMP1, COL1A1 Ca

Language: Английский

Citations

3
Immunolocalization of the mechanogated ion channels PIEZO1 and PIEZO2 in human and mouse dental pulp and periodontal ligament DOI Creative Commons

Juan J. Gaite,

A. Solé-Magdalena,

Yolanda García‐Mesa

et al.

The Anatomical Record, Journal Year: 2023, Volume and Issue: 307(5), P. 1960 - 1968

Published: Nov. 17, 2023

Abstract PIEZO1 and PIEZO2 are essential components of mechanogated ion channels, which required for mechanotransduction biological processes associated with mechanical stimuli. There is evidence the presence in teeth periodontal ligaments, especially cell lines mice, but human studies almost nonexistent. Decalcified permanent mouse molars were processed immunohistochemical detection PIEZO2. Confocal laser microscopy was used to examine co‐localization PIEZO 1 vimentin (a marker differentiated odontoblasts) teeth. In outer layer dental pulp, abundant PIEZO1‐ PIEZO2‐positive cells found that had no odontoblast morphology vimentin‐negative. Based on their morphology, location, absence positivity, they identified as pulp stem or pre‐odontoblasts. However, ubiquitously detected colocalized odontoblasts. Intense immunoreactivity has been observed murine ligaments. Our findings suggest may be mechanosensors/mechanotransducers odontoblasts, well transmission forces by ligament humans mice.

Language: Английский

Citations

7
LncRNA urothelial cancer associated 1 promotes the osteogenic differentiation of human periodontal ligament stem cells by regulating the miR-96–5p/Osx axis DOI
Xuefei Sun,

Yu Pu,

Shaojie Dong

et al.

Archives of Oral Biology, Journal Year: 2023, Volume and Issue: 158, P. 105855 - 105855

Published: Nov. 28, 2023

Language: Английский

Citations

4
Effect of Mechanical Force Stress on the Inflammatory Response in Human Periodontal Ligament Cells DOI Creative Commons
Thira Rojasawasthien, Supreda Suphanantachat Srithanyarat,

Wajathip Bulanawichit

et al.

International Dental Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Human periodontal ligament (hPDL) is continuously exposed to mechanical forces that can induce inflammatory responses in resident stem cells (hPDLSCs). Here, we review the impact of force on hPDLSCs, focusing activation cytokines and related signalling pathways, which subsequently influence tissue remodelling. The effects various forces, including compressive, shear, tensile hPDLSCs are discussed. highlights role pro-inflammatory such as IL-1β, IL-6, TNF-α mediating responses, well counteracting anti-inflammatory like IL-4 IL-10. Additionally, underscore involvement toll-like receptors (TLRs), particularly TLR4, transducing stress signals modulating cytokine production. This demonstrates respond different with specific gene expression changes direct bone remodelling signals, leading increased osteoblast osteoclast activity. Moreover, together contiguous hPDL cells, by regulating immune function several cells. complex relationship between stress, inflammation, cellular response warrants further research develop therapeutic strategies for diseases.

Language: Английский

Citations

1
Piezo1 reduces inflammation regulatory ability of periodontal ligament cells to decelerate orthodontic tooth movement in response to heavy mechanical force DOI Creative Commons
Leilei Zheng, Ye Zhu,

Xuehuan Meng

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 5, 2024

Abstract Periodontal ligament (PDL) cells exhibit immunoregulatory characteristics and are being explored as promising therapeutic candidates for accelerating orthodontic tooth movement (OTM). The impact of light or heavy mechanical force (MF) on PDL cellular regulatory ability the modulation alveolar bone remodeling during OTM is not yet fully understood. Piezo1, a mechanosensitive ion channel, essential mediating conversion in response to MF stimuli. This study aimed investigate influence consequent by altering capabilities through Piezo1 modulation. research represents initial exploration role activation either mitochondrial calcium concentrations mitochondria-associated endoplasmic reticulum membranes cells. results have validated that MF-induced deregulation leads decrease release DNA into cytoplasm, thereby inhibiting cGAS-STING pathway activation. Inhibition capacity OTM, resulting reduced osteoclast deceleration rat sting−/− model. cumulative findings this indicate manipulating via could potentially offer an effective strategy shorten duration treatment.

Language: Английский

Citations

0