The neutrophil–osteogenic cell axis promotes bone destruction in periodontitis DOI Creative Commons
Yutaro Ando, Masayuki Tsukasaki, Nam Cong‐Nhat Huynh

et al.

International Journal of Oral Science, Journal Year: 2024, Volume and Issue: 16(1)

Published: Feb. 27, 2024

Abstract The immune-stromal cell interactions play a key role in health and diseases. In periodontitis, the most prevalent infectious disease humans, immune cells accumulate oral mucosa promote bone destruction by inducing receptor activator of nuclear factor-κB ligand (RANKL) expression osteogenic such as osteoblasts periodontal ligament cells. However, detailed mechanism underlying immune–bone periodontitis is not fully understood. Here, we performed single-cell RNA-sequencing analysis on mouse lesions showed that neutrophil–osteogenic crosstalk involved periodontitis-induced loss. displayed marked infiltration neutrophils, silico analyses suggested neutrophils interacted with through cytokine production. Among cytokines expressed oncostatin M (OSM) potently induced RANKL primary osteoblasts, deletion OSM significantly ameliorated Epigenomic data identified OSM-regulated enhancer region cells, mice lacking this decreased loss while maintaining physiological metabolism. These findings shed light regulation during bacterial infection, highlighting novel osteoimmune crosstalk.

Language: Английский

Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes DOI Creative Commons
Yazhen Li, Lu Yang, Yue Hou

et al.

Bioactive Materials, Journal Year: 2022, Volume and Issue: 18, P. 213 - 227

Published: March 22, 2022

Regenerating periodontal bone tissues in the aggravated inflammatory microenvironment under diabetic conditions is a great challenge. Here, polydopamine-mediated graphene oxide (PGO) and hydroxyapatite nanoparticle (PHA)-incorporated conductive alginate/gelatin (AG) scaffold developed to accelerate regeneration by modulating microenvironment. PHA confers with osteoinductivity PGO provides pathway for scaffold. The promotes transferring endogenous electrical signals cells activating Ca2+ channels. Moreover, nanomaterials has reactive oxygen species (ROS)-scavenging ability anti-inflammatory activity. It also exhibits an immunomodulatory that suppresses M1 macrophage polarization activates M2 macrophages secrete osteogenesis-related cytokines mediating glycolytic RhoA/ROCK pathways macrophages. induces excellent defects of rats because synergistic effects good conductive, ROS-scavenging, anti-inflammatory, abilities. This study fundamental insights into synergistical conductivity, osteoinductivity, abilities on offers novel strategy design biomaterials treatment immune-related diseases tissue regeneration.

Language: Английский

Citations

195
Injectable hydrogels with high drug loading through B–N coordination and ROS-triggered drug release for efficient treatment of chronic periodontitis in diabetic rats DOI
Xiaodan Zhao, Yuxuan Yang, Jing Yu

et al.

Biomaterials, Journal Year: 2022, Volume and Issue: 282, P. 121387 - 121387

Published: Jan. 24, 2022

Language: Английский

Citations

137
The Role of the Immune Microenvironment in Bone Regeneration DOI Creative Commons
Ning Yang, Yao Liu

International Journal of Medical Sciences, Journal Year: 2021, Volume and Issue: 18(16), P. 3697 - 3707

Published: Jan. 1, 2021

Bone is an active tissue, being constantly renewed in healthy individuals with participation of the immune system to a large extent. Any imbalance between processes bone formation and resorption linked various inflammatory diseases. The plays important role tissue resorption. Recently, many studies have demonstrated complex interactions skeletal systems. Both cells cytokines contribute regulation homeostasis, cells, including osteoblasts, osteoclasts, osteocytes, also influence cellular functions cells. These crosstalk mechanisms finally emerged, forming new field research called osteoimmunology. Therefore, microenvironment crucial determining speed outcome healing, repair, regeneration. In this review, we summarise regeneration from aspects cytokines. elucidation involved process would provide therapeutic targets for improving curative effects injury treatment.

Language: Английский

Citations

134
The Bidirectional Relationship between Periodontal Disease and Diabetes Mellitus—A Review DOI Creative Commons

Ioana Păunică,

Marina Cristina Giurgiu, Anca Silvia Dumitriu

et al.

Diagnostics, Journal Year: 2023, Volume and Issue: 13(4), P. 681 - 681

Published: Feb. 11, 2023

Periodontitis is a chronic inflammatory disease caused by the presence of bacterial biofilm known as dental plaque. This affects supporting apparatus teeth, especially periodontal ligaments and bone surrounding teeth. Periodontal diabetes seem to be interrelated in bidirectional relationship, have been increasingly studied recent decades. For example, mellitus has detrimental effect on disease, increasing its prevalence, extent, severity. In turn, periodontitis negatively glycemic control course diabetes. review aims present most recently discovered factors that contribute pathogenesis, therapy, prophylaxis these two diseases. Specifically, article focuses microvascular complications, oral microbiota, pro- anti-inflammatory diabetes, disease. As presented this review, diseases require specific/ complementary therapeutic solutions when they occur association, with new clinical trials epidemiological research being necessary for better interdependent pathogenic topic.

Language: Английский

Citations

86
Dynamically Bioresponsive DNA Hydrogel Incorporated with Dual-Functional Stem Cells from Apical Papilla-Derived Exosomes Promotes Diabetic Bone Regeneration DOI
Xuan Jing, Si Wang, Han Tang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2022, Volume and Issue: 14(14), P. 16082 - 16099

Published: March 28, 2022

The regeneration of bone defects in patients with diabetes mellitus (DM) is remarkably impaired by hyperglycemia and over-expressed proinflammatory cytokines, proteinases (such as matrix metalloproteinases, MMPs), etc. In view the fact that exosomes represent a promising nanomaterial, herein, we reported excellent capacity stem cells from apical papilla-derived (SCAP-Exo) to facilitate angiogenesis osteogenesis whether normal or diabetic conditions vitro. Then, bioresponsive polyethylene glycol (PEG)/DNA hybrid hydrogel was developed support controllable release SCAP-Exo for defects. This system could be triggered elevated pathological cue (MMP-9) response dynamic microenvironment. It further confirmed administration injectable SCAP-Exo-loaded PEG/DNA into mandibular defect rats demonstrated great therapeutic effect on promoting vascularized regeneration. addition, miRNA sequencing suggested mechanism dual-functional might related highly expressed miRNA-126-5p miRNA-150-5p. Consequently, our study provides valuable insights design exosome-delivery systems improve patients.

Language: Английский

Citations

74
Polydopamine-Mediated Immunomodulatory Patch for Diabetic Periodontal Tissue Regeneration Assisted by Metformin-ZIF System DOI

Jinglei Gong,

Chengxinyue Ye,

Jinhui Ran

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(17), P. 16573 - 16586

Published: Aug. 14, 2023

An essential challenge in diabetic periodontal regeneration is achieving the transition from a hyperglycemic inflammatory microenvironment to regenerative one. Here, we describe polydopamine (PDA)-mediated ultralong silk microfiber (PDA-mSF) and metformin (Met)-loaded zeolitic imidazolate framework (ZIF) incorporated into fibroin/gelatin (SG) patch promote soft hard tissue by regulating immunomodulatory microenvironment. The PDA-mSF endows with reactive oxygen species (ROS)-scavenging ability anti-inflammatory activity, reducing response suppressing M1 macrophage polarization. Moreover, PDA improves ligament reconstruction via its cell affinity. Sustained release of Met Met-ZIF system confers antiaging abilities activating M2 polarization secrete osteogenesis-related cytokines, while Zn2+ also promotes bone regeneration. Consequently, creates favorable for These features synergistically accelerate Thus, our findings offer potential therapeutic strategy periodontitis.

Language: Английский

Citations

48
Diabetes mellitus promotes susceptibility to periodontitis—novel insight into the molecular mechanisms DOI Creative Commons

Mingcan Zhao,

Yuandong Xie,

Wenjia Gao

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 16, 2023

Diabetes mellitus is a main risk factor for periodontitis, but until now, the underlying molecular mechanisms remain unclear. can increase pathogenicity of periodontal microbiota and inflammatory/host immune response periodontium. Hyperglycemia induces reactive oxygen species (ROS) production enhances oxidative stress (OS), exacerbating tissue destruction. Furthermore, alveolar bone resorption damage epigenetic changes in induced by diabetes may also contribute to periodontitis. We will review latest clinical data on evidence promoting susceptibility periodontitis from epidemiological, mechanistic, potential therapeutic targets discuss possible mechanistic targets, focusing particular novel OS. Understanding intertwined pathogenesis explain cross-interference between endocrine metabolic inflammatory diseases better, provide theoretical basis new systemic holistic treatment, promote interprofessional collaboration physicians dentists.

Language: Английский

Citations

47
Periodontitis and metabolic diseases (diabetes and obesity): Tackling multimorbidity DOI Creative Commons
Crystal Marruganti, Jean Suvan, Francesco D’Aiuto

et al.

Periodontology 2000, Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 16, 2023

Abstract Noncommunicable diseases (NCDs) are multifactorial, long‐term, chronic conditions that represent a burden to health‐care systems worldwide as they can only be controlled rather than cured; hence, require long‐term care. With the exponential increase in NCDs, occurrence of individuals presenting with more one disease is also rapidly rising. “Multimorbidity,” defined presence two or physical mental disorders, now considered epidemic, affecting around 20% adult population. Periodontitis, diabetes, and obesity, all inflammatory diseases, an example multimorbidity highly relevant dental practitioners. Over last three decades, three‐way relationship among has been vastly researched accepted, important contributions by European researchers. The interplay periodontitis, obesity sustained shared biological mechanisms, such systemic inflammation, insulin resistance, metabolic dysfunction, well common lifestyle‐related risk factors. As such, unhealthy lifestyles were found generally inflammation resistance decrease immune function, eventually increasing NCDs onset development multimorbidity. This narrative review evidence supports need for paradigm shift from “single‐disease” “multiple‐disease” framework, characterized integrated multidisciplinary approach, which should include lifestyle modification interventions successfully tackle multimorbid periodontitis (diabetes obesity). A care pathway both medical settings further global health challenge

Language: Английский

Citations

42
Inhibition of METTL3 Alleviates NLRP3 Inflammasome Activation via Increasing Ubiquitination of NEK7 DOI Creative Commons
Xinyi Zhou, Xiaoyu Yang,

Shenzhen Huang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(26)

Published: May 2, 2024

Abstract N6‐methyladenosine (m 6 A) modification, installed by METTL3‐METTL14 complex, is abundant and critical in eukaryotic mRNA. However, its role oral mucosal immunity remains ambiguous. Periodontitis a special but prevalent infectious disease characterized as hyperinflammation of mucosa bone resorption. Here, it reported that genetic deletion Mettl3 alleviates periodontal destruction via suppressing NLRP3 inflammasome activation. Mechanistically, the stability TNFAIP3 (also known A20) transcript significantly attenuated upon m A modification. When silencing METTL3, accumulated functioning ubiquitin‐editing enzyme facilitates ubiquitination NEK7 [NIMA (never mitosis gene a)‐related kinase 7], subsequently impairs assembly. Furtherly, Coptisine chloride, natural small‐molecule, discovered novel METTL3 inhibitor performs therapeutic effect on periodontitis. The study unveils previously unknown pathogenic mechanism METTL3‐mediated modifications periodontitis indicates potential target.

Language: Английский

Citations

18
Oral Microbiota: A Major Player in the Diagnosis of Systemic Diseases DOI Creative Commons
Charlotte Thomas,

Matthieu Minty,

Alexia Vinel

et al.

Diagnostics, Journal Year: 2021, Volume and Issue: 11(8), P. 1376 - 1376

Published: July 30, 2021

The oral cavity is host to a complex and diverse microbiota community which plays an important role in health disease. Major infections, i.e., caries periodontal diseases, are both responsible for induced by dysbiosis. This dysbiosis known have impact on other chronic systemic whether triggering or aggravating them, making the novel target diagnosing, following, treating diseases. In this review, we summarize major roles that can play disease development aggravation also how tools help investigate ecosystem. Finally, describe new therapeutic approaches based bacterial recolonization modulation therapies. Collaboration diagnosis treatment between specialists general of key importance bridging improving patients' wellbeing.

Language: Английский

Citations

75