CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture DOI Creative Commons
David Forsberg,

Zachi Horn,

Evangelia Tserga

et al.

eLife, Journal Year: 2016, Volume and Issue: 5

Published: June 27, 2016

Inflammation-induced release of prostaglandin E2 (PGE2) changes breathing patterns and the response to CO2 levels. This may have fatal consequences in newborn babies result sudden infant death. To elucidate underlying mechanisms, we present a novel brainstem organotypic culture that generates rhythmic neural network motor activity for 3 weeks. We show increased elicits gap junction-dependent PGE2. alters preBötzinger rhythm-generating complex chemosensitive respiratory regions, thereby increasing sigh frequency depth inspiration. used mice lacking eicosanoid prostanoid receptors (EP3R), slices optogenetic inhibition EP3R+/+ cells demonstrate EP3R is important ventilatory hypercapnia. Our study identifies pathway linking inflammatory systems, with implications inspiration sighs throughout life, ability autoresuscitate when fails.

Language: Английский

Emergent Elements of Inspiratory Rhythmogenesis: Network Synchronization and Synchrony Propagation DOI Creative Commons
Sufyan Ashhad, Jack L. Feldman

Neuron, Journal Year: 2020, Volume and Issue: 106(3), P. 482 - 497.e4

Published: March 3, 2020

Language: Английский

Citations

86
Synchronized Cardiac Impulses Emerge From Heterogeneous Local Calcium Signals Within and Among Cells of Pacemaker Tissue DOI Creative Commons

Rostislav Bychkov,

Magdalena Juhaszova, Kenta Tsutsui

et al.

JACC. Clinical electrophysiology, Journal Year: 2020, Volume and Issue: 6(8), P. 907 - 931

Published: Aug. 1, 2020

Language: Английский

Citations

83
Whisker-Mediated Touch System in Rodents: From Neuron to Behavior DOI Creative Commons
Mehdi Adibi

Frontiers in Systems Neuroscience, Journal Year: 2019, Volume and Issue: 13

Published: Aug. 21, 2019

A key question in systems neuroscience is to identify how sensory stimuli are represented neuronal activity, and the activity of neurons turn ``read out'' by downstream give rise behavior. The choice proper model system adress these questions, hence, a crucial step. Over past decade, increasingly powerful array experimental approaches that has become available non-primate models (e.g. optogenetics two-photon imaging) spurred renewed interest for use rodent research. Here we introduce whisker-mediated touch as structurally well-established well-organized which despite its simplicity gives rises complex behaviors. This serves behaviorally efficient system; known nocturnal animals, along with their olfaction, rodents rely on collect information about surrounding environment. Moreover, this represents well-studied circuitry somatotopic organization: at every stage processing, one can anatomical functional topographic maps whiskers: ``barrelettes'' brainstem nuclei, ``barreloids'' thalamus, ``barrels'' cortex. article provides brief review basic anatomy function whisker rodents.

Language: Английский

Citations

78
Defining the Rhythmogenic Elements of Mammalian Breathing DOI Open Access
Jan‐Marino Ramirez, Nathan A. Baertsch

Physiology, Journal Year: 2018, Volume and Issue: 33(5), P. 302 - 316

Published: Aug. 15, 2018

Breathing's remarkable ability to adapt changes in metabolic, environmental, and behavioral demands stems from a complex integration of its rhythm-generating network within the wider nervous system. Yet, this complicates identification specific rhythmogenic elements. Based on principles learned smaller rhythmic networks invertebrates, we define criteria that identify elements mammalian breathing discuss how they interact produce robust, dynamic breathing.

Language: Английский

Citations

75
Inhibition, Not Excitation, Drives Rhythmic Whisking DOI Creative Commons
Martin Deschênes, Jun Takatoh,

Anastasia Kurnikova

et al.

Neuron, Journal Year: 2016, Volume and Issue: 90(2), P. 374 - 387

Published: April 1, 2016

Language: Английский

Citations

74
Interactions between respiratory oscillators in adult rats DOI Creative Commons
Robert Huckstepp, Lauren E. Henderson, Kathryn Cardoza

et al.

eLife, Journal Year: 2016, Volume and Issue: 5

Published: June 13, 2016

Breathing in mammals is hypothesized to result from the interaction of two distinct oscillators: preBötzinger Complex (preBötC) driving inspiration and lateral parafacial region (pFL) active expiration. To understand interactions between these oscillators, we independently altered their excitability spontaneously breathing vagotomized urethane-anesthetized adult rats. Hyperpolarizing preBötC neurons decreased inspiratory activity initiated expiration, ultimately progressing apnea, i.e., cessation both Depolarizing pFL produced expiration at rest, but not when was suppressed by hyperpolarizing neurons. We conclude that anesthetized rats driven requires an additional form network excitation, ongoing rhythmic sufficient drive motor output or increased chemosensory drive. The organization this coupled oscillator system, which essential for life, may have implications other neural networks contain multiple rhythm/pattern generators.

Language: Английский

Citations

66
A spatially dynamic network underlies the generation of inspiratory behaviors DOI Creative Commons
Nathan A. Baertsch, Liza J. Severs, Tatiana M. Anderson

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2019, Volume and Issue: 116(15), P. 7493 - 7502

Published: March 27, 2019

Significance Breathing is a vital rhythmic behavior that originates from neural networks within the brainstem. It hypothesized breathing rhythm generated by spatially distinct localized to discrete kernels or compartments. Here, we provide evidence functional boundaries of these compartments expand and contract dynamically based on behavioral physiological demands. The ability change in size may allow be very reliable, yet flexible enough accommodate large repertoire mammalian behaviors must integrated with breathing.

Language: Английский

Citations

60
Dynamical disease: Challenges for nonlinear dynamics and medicine DOI
Leon Glass

Chaos An Interdisciplinary Journal of Nonlinear Science, Journal Year: 2015, Volume and Issue: 25(9)

Published: March 24, 2015

Dynamical disease refers to illnesses that are associated with striking changes in the dynamics of some bodily function. There is a large literature mathematics and physics which proposes mathematical models for physiological systems carries out analyses properties these using nonlinear concepts involving stability bifurcations attractors. This paper discusses how can be applied medicine.

Language: Английский

Citations

61
Dbx1 Pre-Bötzinger Complex Interneurons Comprise the Core Inspiratory Oscillator for Breathing in Unanesthetized Adult Mice DOI Creative Commons
Nikolas C. Vann, Francis D. Pham, Kaitlyn E. Dorst

et al.

eNeuro, Journal Year: 2018, Volume and Issue: 5(3), P. ENEURO.0130 - 18.2018

Published: May 1, 2018

The brainstem pre-Bötzinger complex (preBötC) generates inspiratory breathing rhythms, but which neurons comprise its rhythmogenic core? Dbx1-derived may play the preeminent role in rhythm generation, an idea well founded at perinatal stages of development incompletely evaluated adulthood. We expressed archaerhodopsin or channelrhodopsin Dbx1 preBötC intact adult mice to interrogate their function. Prolonged photoinhibition slowed down stopped breathing, whereas prolonged photostimulation sped up breathing. Brief inspiratory-phase evoked next breath earlier than expected, brief expiratory-phase delayed subsequent breath. Conversely, increased duration and breath, expected. Because they govern frequency precise timing breaths awake with sensorimotor feedback intact, constitute essential core component oscillator, knowledge directly relevant human health physiology.

Language: Английский

Citations

54
Spinal Shox2 interneuron interconnectivity related to function and development DOI Creative Commons
Ngoc T. Ha, Kimberly J. Dougherty

eLife, Journal Year: 2018, Volume and Issue: 7

Published: Dec. 31, 2018

Neuronal networks generating hindlimb locomotion are located in the spinal cord. The mechanisms underlying rhythmogenesis unknown but network activity and interconnectivity of excitatory interneurons likely play prominent roles. Here, we investigate within Shox2 interneuron population, a subset which has been suggested to be involved locomotor rhythm generation, using paired recordings isolated cords or slices from transgenic mice. Sparse unidirectional connections consistent with chemical synaptic transmission bidirectional mediated by electrical synapses were present distinct subsets interneurons. Moreover, preferentially found between functionally-related Though prevalent neonatal mice, coupling began decline incidence strength mice ~ 3 weeks age. Overall, our data suggest that gap junctional promotes synchronization interneurons, may implicated rhythmicity developing

Language: Английский

Citations

50