Quantifying motion artifacts in diffuse in vivo flow cytometry (DIFC) and design of a wearable device for human DIFC DOI Open Access

Mark Kenneth Kellish

Published: Jan. 1, 2023

DiFC is an optical technique developed by our group for identifying and monitoring the flow of circulating tumor cells (CTCs) other rare in vasculature vivo. We are currently able to detect fluorescently labelled CTCs anesthetized mice superficial vessels tail hind limb, but system prone motion artifacts stemming from involuntary muscle movements cardiac respiratory cycles. The goal this work quantify sensitivity using a custom built 3-axis simulating arm phantom, develop wearable device human that minimizes artifacts. results investigational phantom study suggest sub-millimeter displacements between probe tips tissue higher background, lower signal-to-noise (SNR) ratio increased false alarm rate. designed minimize these probe-tissue compared effectiveness original system.--Author's abstract

Language: Английский

Current Strategies in Photodynamic Therapy (PDT) and Photodynamic Diagnostics (PDD) and the Future Potential of Nanotechnology in Cancer Treatment DOI Creative Commons

Marta Olszowy,

Martyna Nowak-Perlak, Marta Woźniak

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(6), P. 1712 - 1712

Published: June 12, 2023

Photodynamic diagnostics (PDD) and photodynamic therapy (PDT) are well-established medical technologies used for the diagnosis treatment of malignant neoplasms. They rely on use photosensitizers, light oxygen to visualize or eliminate cancer cells. This review demonstrates recent advancements in these modalities with nanotechnology, including quantum dots as innovative photosensitizers energy donors, liposomes micelles. Additionally, this literature explores combination PDT radiotherapy, chemotherapy, immunotherapy, surgery treating various The article also focuses latest achievements PDD enhancements, which seem be very promising field oncology.

Language: Английский

Citations

28
Treatment of Acute Liver Injury through Selective Tropism of High Mobility Group Box 1 Gene-Silenced Large Peritoneal Macrophages DOI Creative Commons

Dhaval Oza,

Fernando Ivich,

Kirsten Deprey

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Tissue-resident macrophages (TRMs) are attractive cells to therapeutically deliver oligonucleotide and other gene-expression modifying modalities treat a wide array of diseases ranging from inflammatory autoimmune, even cancer. Here, we focus on TRMs located inside the peritoneal cavity lining abdomen that selectively express transcription factor GATA6 called large (GLPMs) successfully demonstrate functional GLPM-selective delivery Cy5-fluorophore-labeled siRNA encapsulated in C12–200 cationic-lipidoid-based nanoparticles (siRNA-Cy5 (C12–200)). Despite being TRMs, GLPMs possess specific migratory ability peritoneally liver tissue upon injury incited by acetaminophen (APAP) overdose mice. A rapid, injury-driven tropism carrying siRNA-Cy5 (C12–200) was seen via systemic circulation, which elegantly demonstrated using noninvasive live-cell tracking technique diffuse vivo flow cytometry (DiFC). Finally, RNAi-mediated silencing well-known pro-inflammatory damage-associated molecular pattern (DAMP) High Mobility Group Box-1 (HMGB1) gene led mitigation inflammation prevention GLPM modulation state, further translated into significant protection APAP-driven reduction circulating cytokines owing muted response acute injury. Moreover, HMGB1 GalNAc-conjugated hepatocyte-targeting did not reciprocate findings, solidifying our results. Together, data suggested act as carriers rapidly bringing lipid nanoparticle-encapsulated RNAi injured have emerged viable strategy address diseases, especially those more nature.

Language: Английский

Citations

0
Noninvasively Real‐Time Monitoring In‐Vivo Immune Cell and Tumor Cell Interaction by NIR‐II Nanosensor DOI Open Access
Liwen Huang, Ming Jiang,

Zhihua Wang

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Abstract Immunocytotherapy holds significant promise as a novel cancer treatment, but its effectiveness is often hindered by delayed responses, requiring evaluations every 2–3 weeks based on current diagnostic methods. Early assessment of immune cell‐tumor cell interactions could provide more timely insights into therapeutic efficacy, enabling adjustments to treatment plans. In this study, noninvasive nanosensor (C8R‐DSNP) for real‐time monitoring in vivo activities the second near‐infrared long‐wavelength (NIR‐II‐L) window (1500–1900 nm), which offers deep tissue transparency, reported. The C8R‐DSNP responds rapidly caspase‐8, key apoptotic signaling molecule generated during between natural killer (NK‐92) cells and tumor cells. Using ratiometric NIR‐II‐L fluorescence imaging, dynamic observations NK‐92 cells' engagement with mouse model are captured. These results demonstrate apoptosis that happens early 4.5 h after infusion. Additionally, vitro urine imaging confirmed initiation via cleaved fluorescent small molecules, while single‐cell tracking within blood vessels tumors further elucidated dynamics. This approach valuable optimizing immunocytotherapy strategies.

Language: Английский

Citations

0
Signal and measurement considerations for human translation of diffuse in vivo flow cytometry DOI Creative Commons
Fernando Ivich, Joshua Pace, Amber Williams

et al.

Journal of Biomedical Optics, Journal Year: 2022, Volume and Issue: 27(06)

Published: June 20, 2022

Significance: “Diffuse in vivo flow cytometry” (DiFC) is an emerging technology for fluorescence detection of rare circulating cells directly large deep-seated blood vessels mice. Because DiFC uses highly scattered light, principle, it could be translated to human use. However, open question whether fluorescent signals from single would detectable human-scale anatomies. Aim: Suitable a wrist or forearm are at depth ∼2 4 mm. The aim this work was study the impact instrument geometry and wavelength on detected signal maximum moving cell. Approach: We used Monte Carlo simulations compute Jacobian (sensitivity) matrices range source detector separations (SDS) tissue optical properties over visible near infrared spectrum. performed experimental measurements with three available versions (488, 640, 780 nm), microspheres, mimicking phantoms. both computational data estimate each combination settings. Results: For problem, our analysis showed that vessels, sensitivity obtained NIR light (780 nm) 3-mm SDS. Conclusions: These results suggest that—in suitable molecularly targeted probes—circulating nanosensors could, circulation humans.

Language: Английский

Citations

12
Two-color diffuse in vivo flow cytometer DOI Creative Commons
Amber Williams,

Augustino V. Scorzo,

Rendall R. Strawbridge

et al.

Journal of Biomedical Optics, Journal Year: 2024, Volume and Issue: 29(06)

Published: May 30, 2024

Hematogenous metastasis is mediated by circulating tumor cells (CTCs) and CTC clusters (CTCCs). We recently developed "diffuse

Language: Английский

Citations

2
Dual-ratio approach for detection of point fluorophores in biological tissue DOI Creative Commons
Giles Blaney, Fernando Ivich, Angelo Sassaroli

et al.

Journal of Biomedical Optics, Journal Year: 2023, Volume and Issue: 28(07)

Published: July 22, 2023

Diffuse in-vivo Flow Cytometry (DiFC) is an emerging fluorescence sensing method to non-invasively detect labeled circulating cells in-vivo. However, due Signal-to-Noise Ratio (SNR) constraints largely attributed background tissue autofluorescence, DiFC's measurement depth limited. multiplies Aim: The Dual-Ratio (DR) / dual-slope a new optical that aims suppress noise and enhance SNR deep regions. We aim investigate the combination of DR Near-InfraRed (NIR) DiFC improve cells' maximum detectable SNR.

Language: Английский

Citations

5
Ratiometric fluorescence sensing and quantification of circulating blood sodium sensors in mice in vivo DOI Creative Commons
Fernando Ivich, Isen Andrew C. Calderon, Qianqian Fang

et al.

Biomedical Optics Express, Journal Year: 2023, Volume and Issue: 14(11), P. 5555 - 5555

Published: Sept. 26, 2023

In this work, we introduce ratiometric diffuse in vivo flow cytometry (R-DiFC) for quantitative measurement of circulating fluorescent red blood cell (fRBC) sensors systemic sodium levels. Unlike our previous work measuring fRBC sensors, R-DiFC allows simultaneous two fluorophores encapsulated the sensor, ratio which enables self-calibration fluorescence signal with different depths biological tissue. We show that varies significantly less than either alone. This holds promise personalized monitoring bipolar patients future.

Language: Английский

Citations

5
Research progress on the detection of circulating tumor cells by in vivo flow cytometry DOI
Yuanhao Zhang,

Jiaming Ge,

Zhaowei Liu

et al.

Optics and Lasers in Engineering, Journal Year: 2024, Volume and Issue: 186, P. 108731 - 108731

Published: Dec. 7, 2024

Language: Английский

Citations

1
Effect of pulsed laser parameters on photoacoustic flow cytometry efficiency in vitro and in vivo DOI
Oleg V. Grishin, Natalia A. Shushunova, Daniil N. Bratashov

et al.

Cytometry Part A, Journal Year: 2023, Volume and Issue: 103(11), P. 868 - 880

Published: July 17, 2023

Photoacoustic flow cytometry is one of the most effective approaches to detect "alien" objects in bloodstream, including circulating tumor cells, blood clots, parasites, and emboli. However, possibility detecting high-amplitude signals from these against background depends on parameters laser pulse. So, dependencies photoacoustic amplitude number pulse energy (5-150 μJ), length (1, 2, 5 ns), repetition rate (2, 5, 10 kHz) for melanoma cells were investigated. First, PA responses a cell suspension vitro measured directly assess efficiency converting light into an acoustic signal. After it, same dependence with developed murine model based constant injection animal was tested. Both vivo experiments show that signal generation increases above 15 μJ. Shorter pulses, especially 1 ns, provide more efficient as well higher rates. A also provides generation, but leads overheating skin. The results limits where system can be effectively used detection undiluted both models.

Language: Английский

Citations

2
In Vivo Labeling and Detection of Circulating Tumor Cells in Mice Using OTL38 DOI Creative Commons
Joshua Pace, Jane Lee, Madduri Srinivasarao

et al.

Molecular Imaging and Biology, Journal Year: 2024, Volume and Issue: 26(4), P. 603 - 615

Published: April 9, 2024

Abstract Purpose We recently developed an optical instrument to non-invasively detect fluorescently labeled circulating tumor cells (CTCs) in mice called ‘Diffuse vivo Flow Cytometry’ (DiFC). OTL38 is a folate receptor (FR) targeted near-infrared (NIR) contrast agent that FDA approved for use fluorescence guided surgery of ovarian and lung cancer. In this work, we investigated the labeling detection FR + CTCs with DiFC. Procedures tested cancer cell lines (IGROV-1 L1210A) as well FR- MM.1S suspensions Human Peripheral Blood Mononuclear (PBMCs) vitro . also NIR-DIFC L1210A blood circulation nude vivo. Results 62% IGROV-1 83% were above non-specific background levels PBMCs compared only 2% cells. could be directly externally detected using NIR-DiFC low false positive rates. Conclusions This work shows feasibility Although further refinement DiFC signal processing algorithms testing other animal models needed, may eventually pave way human

Language: Английский

Citations

0