Cell membrane coating integrity affects the internalization mechanism of biomimetic nanoparticles

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Sept. 30, 2021

Cell membrane coated nanoparticles (NPs) have recently been recognized as attractive nanomedical tools because of their unique properties such immune escape, long blood circulation time, specific molecular recognition and cell targeting. However, the integrity coating on NPs, a key metrics related to quality these biomimetic-systems resulting biomedical function, has remained largely unexplored. Here, we report fluorescence quenching assay probe coating. In contradiction common assumption perfect coating, uncover that up 90% biomimetic NPs are only partially coated. Using in vitro homologous targeting studies, demonstrate could still be internalized by target cells. By combining simulations with experimental analysis, further identify an endocytic entry mechanism for NPs. We unravel high degree (≥50%) enter cells individually, whereas low (<50%) need aggregate together before internalization. This quantitative method fundamental understanding how will enhance rational designing nanosystems pave way more effective cancer nanomedicine.

Language: Английский

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Recent progress in targeted delivery vectors based on biomimetic nanoparticles

Signal Transduction and Targeted Therapy, Journal Year: 2021, Volume and Issue: 6(1)

Published: June 7, 2021

Abstract Over the past decades, great interest has been given to biomimetic nanoparticles (BNPs) since rise of targeted drug delivery systems and nanotechnology. Biological vectors including cell membranes, extracellular vesicles (EVs), viruses are considered promising candidates for owing their biocompatibility biodegradability. BNPs, integration biological functional agents, anticipated load cargos or camouflage synthetic achieve delivery. Despite excellent intrinsic properties, natural deliberately modified endow multiple functions such as good permeability, improved loading capability, high specificity. Through structural modification transformation vectors, they pervasively utilized more effective vehicles that can deliver contrast chemotherapy drugs, nucleic acids, genes target sites refractory disease therapy. This review summarizes recent advances in based on EVs, viruses, highlighting potential applications BNPs fields biomedical imaging therapy industry, well discussing possibility clinical translation exploitation trend these BNPs.

Language: Английский

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Cell membrane coated nanocarriers - an efficient biomimetic platform for targeted therapy

Journal of Controlled Release, Journal Year: 2020, Volume and Issue: 327, P. 546 - 570

Published: Sept. 8, 2020

Language: Английский

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Harnessing Endogenous Stimuli for Responsive Materials in Theranostics

ACS Nano, Journal Year: 2021, Volume and Issue: 15(2), P. 2068 - 2098

Published: Feb. 8, 2021

Materials that respond to endogenous stimuli are being leveraged enhance spatiotemporal control in a range of biomedical applications from drug delivery diagnostic tools. The design materials undergo morphological or chemical changes response specific biological cues pathologies will be an important area research for improving efficacies existing therapies and imaging agents, while also promising developing personalized theranostic systems. Internal stimuli-responsive systems can engineered across length scales nanometers macroscopic signals such as enzymes, pH, glucose, ATP, hypoxia, redox signals, nucleic acids by incorporating synthetic bio-inspired moieties natural building blocks. This Review summarize mechanisms fabrication strategies used internal with focus on broad pathologies, including cancer, diabetes, vascular disorders, inflammation, microbial infections. We discuss observed challenges, future directions, clinical translation aspects these responsive materials.

Language: Английский

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Advances in nanomaterial-based targeted drug delivery systems

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: April 13, 2023

Nanomaterial-based drug delivery systems (NBDDS) are widely used to improve the safety and therapeutic efficacy of encapsulated drugs due their unique physicochemical biological properties. By combining with nanoparticles using rational targeting pathways, nano-targeted were created overcome main drawbacks conventional treatment, including insufficient stability solubility, lack transmembrane transport, short circulation time, undesirable toxic effects. Herein, we reviewed recent developments in different design strategies approaches employing various nanomaterial-based systems. We also discussed challenges perspectives smart precisely intravascular extravascular diseases.

Language: Английский

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Cell membrane-derived vesicles for delivery of therapeutic agents

Acta Pharmaceutica Sinica B, Journal Year: 2021, Volume and Issue: 11(8), P. 2096 - 2113

Published: Feb. 2, 2021

Cell membranes have recently emerged as a new source of materials for molecular delivery systems. been extruded or sonicated to make nanoscale vesicles. Unlike synthetic lipid polymeric nanoparticles, cell membrane-derived vesicles unique multicomponent feature, comprising lipids, proteins, and carbohydrates. Because contain the intrinsic functionalities signaling networks their parent cells, they can overcome various obstacles encountered

Language: Английский

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Cell membrane-coated nanoparticles: a novel multifunctional biomimetic drug delivery system

Drug Delivery and Translational Research, Journal Year: 2022, Volume and Issue: 13(3), P. 716 - 737

Published: Nov. 22, 2022

Language: Английский

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Brain Co‐Delivery of Temozolomide and Cisplatin for Combinatorial Glioblastoma Chemotherapy

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(33)

Published: June 23, 2022

Glioblastoma (GBM) is an intractable malignancy with high recurrence and mortality. Combinatorial therapy based on temozolomide (TMZ) cisplatin (CDDP) shows promising potential for GBM in clinical trials. However, significant challenges include limited blood-brain-barrier (BBB) penetration, poor targeting of tissue/cells, systemic side effects, which hinder its efficacy therapy. To surmount these challenges, new GBM-cell membrane camouflaged pH-sensitive biomimetic nanoparticles (MNPs) inspired by the fact that cancer cells readily pass BBB localize homologous cells, are developed. This study's results show MNPs can efficiently co-load TMZ CDDP, transport across to specifically target GBM. Incorporation polymer then allows controlled release drug cargos at sites combination Mice bearing orthotopic U87MG or drug-resistant U251R tumor treated MNPs@TMZ+CDDP a potent anti-GBM effect, greatly extending survival time relative mice receiving single-drug loaded nanoparticles. No obvious effects apparent histological analyses blood routine studies. Considering results, nanoparticle formulation overcomes multiple currently limiting combined CDDP appears be strategy future combinatorial chemotherapy.

Language: Английский

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Advances in Drug Delivery Systems Based on Red Blood Cells and Their Membrane-Derived Nanoparticles

ACS Nano, Journal Year: 2023, Volume and Issue: 17(6), P. 5187 - 5210

Published: March 10, 2023

Red blood cells (RBCs) and RBC membrane-derived nanoparticles have been historically developed as bioinspired drug delivery systems to combat the issues of premature clearance, toxicity, immunogenicity synthetic nanocarriers. RBC-based possess characteristics including biocompatibility, biodegradability, long circulation time, which make them suited for systemic administration. Therefore, they employed in designing optimal formulations various preclinical models clinical trials treat a wide range diseases. In this review, we provide an overview biology, synthesis, characterization based on RBCs their membrane whole RBCs, membrane-camouflaged nanoparticles, RBC-derived extracellular vesicles, hitchhiking. We also highlight conventional latest engineering strategies, along with therapeutic modalities, enhanced precision effectiveness delivery. Additionally, focus current state applications translation carriers, well discussing opportunities challenges associated these systems.

Language: Английский

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Systematic design of cell membrane coating to improve tumor targeting of nanoparticles

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Oct. 19, 2022

Cell membrane (CM) coating technology is increasingly being applied in nanomedicine, but the entire procedure including adsorption, rupture, and fusion not completely understood. Previously, we showed that majority of biomimetic nanoparticles (NPs) were only partially coated, mechanism underlying this partial remains unclear, which hinders further improvement technique. Here, show an intermediate state due to adsorption CM fragments or vesicles, latter could eventually be ruptured under external force. Such difficult self-repair achieve full limited fluidity. Building on our understanding detailed process, develop a general approach for fixing coating: phospholipid introduced as helper increase fluidity, promoting final lipid patches. The NPs coated with have high ratio (~23%) exhibit enhanced tumor targeting ability comparison traditionally (full ~6%). Our results provide mechanistic basis towards enhancing accumulation.

Language: Английский

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