Compressible viscoelasticity of cell membranes determined by gigahertz-frequency acoustic vibrations DOI Creative Commons
Kuai Yu, Yiqi Jiang,

Yungao Chen

et al.

Photoacoustics, Journal Year: 2023, Volume and Issue: 31, P. 100494 - 100494

Published: April 12, 2023

Membrane viscosity is an important property of cell biology, which determines cellular function, development and disease progression. Various experimental computational methods have been developed to investigate the mechanics cells. However, there no measurements membrane at high-frequencies in live High frequency are because they can probe viscoelastic effects. Here, we gigahertz-frequencies through damping acoustic vibrations gold nanoplates. The experiments modeled using a continuum theory reveals that membranes display viscoelasticity, with estimated relaxation time ca. 5.7+2.4/-2.7 ps. We further demonstrate viscoelasticity be used differentiate cancerous line (the human glioblastoma cells LN-18) from normal mouse brain microvascular endothelial bEnd.3). LN-18 lower than healthy bEnd.3 by factor three. results indicate promising applications characterizing gigahertz-frequency diagnosis.

Language: Английский

Brillouin microscopy DOI
Irina V. Kabakova, Jitao Zhang, Yuchen Xiang

et al.

Nature Reviews Methods Primers, Journal Year: 2024, Volume and Issue: 4(1)

Published: Feb. 1, 2024

Language: Английский

Citations

24
Fundamentals and Advances in Stimuli-Responsive Hydrogels and Their Applications: A Review DOI Creative Commons
Iryna Protsak, Yevhenii M. Morozov

Gels, Journal Year: 2025, Volume and Issue: 11(1), P. 30 - 30

Published: Jan. 2, 2025

This review summarizes the fundamental concepts, recent advancements, and emerging trends in field of stimuli-responsive hydrogels. While numerous reviews exist on this topic, continues to evolve dynamically, certain research directions are often overlooked. To address this, we classify hydrogels based their response mechanisms provide an in-depth discussion key properties mechanisms, including swelling kinetics, mechanical properties, biocompatibility/biodegradability. We then explore hydrogel design, synthesis, structural engineering, followed by overview applications that relatively well established from a scientific perspective, biomedical uses (biosensing, drug delivery, wound healing, tissue engineering), environmental (heavy metal phosphate removal environment polluted water), soft robotics actuation. Additionally, highlight unconventional such as local micro-thermometers cell mechanotransduction. concludes with current challenges future prospects field, aiming inspire further innovations advancements bring them closer societal needs.

Language: Английский

Citations

6
Advances in Brillouin–Mandelstam light-scattering spectroscopy DOI
Fariborz Kargar, Alexander A. Balandin

Nature Photonics, Journal Year: 2021, Volume and Issue: 15(10), P. 720 - 731

Published: July 22, 2021

Language: Английский

Citations

67
Correlative all-optical quantification of mass density and mechanics of subcellular compartments with fluorescence specificity DOI Creative Commons
Raimund Schlüßler, Kyoohyun Kim,

Martin Nötzel

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Jan. 10, 2022

Quantitative measurements of physical parameters become increasingly important for understanding biological processes. Brillouin microscopy (BM) has recently emerged as one technique providing the 3D distribution viscoelastic properties inside samples − so far relying on implicit assumption that refractive index (RI) and density can be neglected. Here, we present a novel method (FOB microscopy) combining BM with optical diffraction tomography epifluorescence imaging explicitly measuring shift, RI, absolute specificity to fluorescently labeled structures. We show neglecting RI might lead erroneous conclusions. Investigating nucleoplasm wild-type HeLa cells, find it lower but higher longitudinal modulus than cytoplasm. Thus, is not merely sensitive water content sample postulate vividly discussed in field. demonstrate further utility FOB various systems including adipocytes intracellular membraneless compartments. provide unexpected scientific discoveries shed quantitative light processes such phase separation transition living cells.

Language: Английский

Citations

63
Brillouin light scattering anisotropy microscopy for imaging the viscoelastic anisotropy in living cells DOI
Hamid Keshmiri, Domagoj Cikes, Marketa Samalova

et al.

Nature Photonics, Journal Year: 2024, Volume and Issue: 18(3), P. 276 - 285

Published: Jan. 18, 2024

Language: Английский

Citations

14
Mechanical mapping of mammalian follicle development using Brillouin microscopy DOI Creative Commons
Chii Jou Chan, Carlo Bevilacqua, Robert Prevedel

et al.

Communications Biology, Journal Year: 2021, Volume and Issue: 4(1)

Published: Sept. 27, 2021

Abstract In early mammalian development, the maturation of follicles containing immature oocytes is an important biological process as functional provide bulk genetic and cytoplasmic materials for successful reproduction. Despite recent work demonstrating regulatory role mechanical stress in oocyte growth, quantitative studies ovarian properties remain lacking both vivo ex vivo. this work, we quantify material ooplasm, connective tissues intact mouse ovaries at distinct stages follicle development using Brillouin microscopy, a non-invasive tool to probe mechanics three-dimensional (3D) tissues. We find that cortex its interior stroma have associated with extracellular matrix deposition, intra-follicular compartments emerge during maturation. Our provides alternative approach study morphogenesis might pave way future understanding mechanotransduction reproductive biology, potential implications infertility diagnosis treatment.

Language: Английский

Citations

40
Diagnostic potential of blood plasma longitudinal viscosity measured using Brillouin light scattering DOI Creative Commons
Jennifer Illibauer,

Tamara Clodi-Seitz,

Alexander Zoufaly

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(33)

Published: Aug. 1, 2024

Blood plasma viscosity (PV) is an established biomarker for numerous diseases. Measurement of the shear PV using conventional rheological techniques is, however, time consuming and requires significant volumes. Here, we show that Brillouin light scattering (BLS) angle-resolved spectroscopy measurements longitudinal from microliter-sized volumes can serve as a proxy measured viscometers. This not trivial given distinct frequency regime probed viscosity, combination bulk representing unique material property on account latter. We demonstrate this healthy persons patients suffering different severities COVID-19 (CoV), which has been associated with increased PV. further additional information contained in BLS-measured effective its temperature scaling provide insight into chemical constituents physical properties be diagnostic value. In particular, find changes are consistent suspension concentration CoV patient samples at elevated temperatures correlated disease severity progression. supported by results rapid BLS spatial-mapping, measurements, elastic scattering, anomalies viscosity. Finally, introduce compact probe to rapidly perform plastic transport tubes. Our open broad avenue diagnostics based high-frequency means implementation.

Language: Английский

Citations

5
Directing Multicellular Organization by Varying the Aspect Ratio of Soft Hydrogel Microwells DOI Creative Commons
Gayatri Pahapale, Jiaxiang Tao, Miloš Nikolić

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(17)

Published: April 17, 2022

Multicellular organization with precise spatial definition is essential to various biological processes, including morphogenesis, development, and healing in vascular other tissues. Gradients patterns of chemoattractants are well-described guides multicellular organization, but the influences 3D geometry soft hydrogels less well defined. Here, discovery a new mode endothelial cell self-organization guided by combinatorial effects stiffness geometry, independent protein or chemical patterning, described. Endothelial cells 2 kPa microwells found be ≈30 times more likely migrate edge organize ring-like than stiff 35 microwells. This curvature significantly pronounced aspect ratio (perimeter/depth) < 25. Physical factors substrates that drive this behavior systematically investigated mathematical model explains balancing dynamic interaction between tangential cytoskeletal tension, cell-cell, cell-substrate adhesion presented. These findings demonstrate importance complex cellular can leveraged facilitate engineering bionics integrated organoid systems customized nutrient networks.

Language: Английский

Citations

22
Pulsed stimulated Brillouin microscopy DOI Creative Commons
Desmond M. Chow, Seok Hyun Yun

Optics Express, Journal Year: 2023, Volume and Issue: 31(12), P. 19818 - 19818

Published: May 18, 2023

Stimulated Brillouin scattering is an emerging technique for probing the mechanical properties of biological samples. However, nonlinear process requires high optical intensities to generate sufficient signal-to-noise ratio (SNR). Here, we show that SNR stimulated can exceed spontaneous with same average power levels suitable We verify theoretical prediction by developing a novel scheme using low duty cycle, nanosecond pulses pump and probe. A shot noise-limited over 1000 was measured total 10 mW 2 ms or 50 200 µs integration on water High-resolution maps frequency shift, linewidth, gain amplitude from cells in vitro are obtained spectral acquisition time 20 ms. Our results demonstrate superior pulsed microscopy.

Language: Английский

Citations

12
High-speed impulsive stimulated Brillouin microscopy DOI
Jiarui Li, Taoran Le, Hongyuan Zhang

et al.

Photonics Research, Journal Year: 2024, Volume and Issue: 12(4), P. 730 - 730

Published: Feb. 1, 2024

Brillouin microscopy, which maps the elastic modulus from frequency shift of scattered light, has evolved to a faster speed for investigation rapid biomechanical changes. Impulsive stimulated scattering (ISBS) spectroscopy potential up measurement through resonant amplification interaction pulsed excitation and time-domain continuous detection. However, significant progress not been achieved due limitation in signal-to-noise ratio (SNR) corresponding need excessive averaging maintain high spectral precision. Moreover, limited spatial resolution also hinders its application mechanical imaging. Here, by scrutinizing SNR model, we design high-speed ISBS microscope multi-parameter optimization including phase, reference power, acquisition time. Leveraging this, with further assistance Matrix Pencil method data processing, three-dimensional images are mapped under multiple contrast mechanisms millimeter-scale polydimethylsiloxane pattern immersed methanol, enabling identification these two transparent materials without any contact or labeling. Our experimental results demonstrate capability precision at sub-millisecond integration time one pixel. With two-order improvement tenfold over state-of-the-art systems, this makes it possible microscopes sensitively investigate changes space.

Language: Английский

Citations

4