Photothermal Nanozyme‐Based Microneedle Patch against Refractory Bacterial Biofilm Infection via Iron‐Actuated Janus Ion Therapy

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(51)

Published: Oct. 14, 2022

Owing to high antibiotic resistance and thermotolerance, bacterial biofilm infections (BBIs) are refractory elimination. Iron is essential for growth metabolism, bacteria can thus accumulate iron from surrounding cells maintain formation survival. Consequently, deficiency in the microenvironment (BME) leads functional failure of innate immune cells. Herein, a novel antibiofilm strategy iron-actuated Janus ion therapy (IJIT) proposed regulate metabolism both A BME-responsive photothermal microneedle patch (FGO@MN) synthesized by Fe3 O4 nanoparticles on graphene oxide nanosheets then encapsulated methacrylated hyaluronic acid needle tips. The catalytic product ·OH FGO@MN BME disrupts heat-shock proteins, coercing thermal sensitization. As synergistic mild treatment triggers uptake, intracellular overload further induces ferroptosis-like death. Moreover, iron-nourished neutrophils around be rejuvenated reactivating suppressed function. Thus, more than 95% BBIs elimination achieved combining heat stress-triggered interference with iron-nutrient reactivation. Furthermore, vivo experiments validate scavenging BBI after 15 days, suggesting promising perspective IJIT future clinical application.

Language: Английский

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Dual‐Responsive Nanocomposites for Synergistic Antibacterial Therapies Facilitating Bacteria‐Infected Wound Healing

Advanced Healthcare Materials, Journal Year: 2022, Volume and Issue: 12(6)

Published: Nov. 14, 2022

Abstract The rising dangers of bacterial infections have created an urgent need for the development a new generation antibacterial technologies and therapeutics. Antibacterial photodynamic therapy (PDT), considered as noninvasive treatment with no drug resistance, has become promising photochemistry‐involved strategy. Titanium oxide (TiO 2 ) is proved to be very efficient PDT agent among photosensitive materials, while property large bandgap TiO makes it only excited by ultraviolet light, which harmful organisms. In this work, novel ligand‐to‐metal charge transfer (LMCT) mediated strategy proposed via harmless near‐infrared light irradiation. By choosing mussel‐inspired material, polydopamine (PDA) involved in forming mesoporous @PDA nanoparticles (mTiO NPs). catechol groups PDA can attach tightly even colloidal environments, also form LMCT bridge, exciting exert function 808 nm Combining sonodynamic (SDT) photothermal properties PDA, simple structure mTiO enables synergistic applications multiple functions under dual excitation NIR ultrasound. This reliable all‐in‐one NPs achieve great effect rapid repair infected wounds.

Language: Английский

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A Hybrid Hydrogel with Intrinsic Immunomodulatory Functionality for Treating Multidrug-Resistant Pseudomonas aeruginosa Infected Diabetic Foot Ulcers

ACS Materials Letters, Journal Year: 2024, Volume and Issue: 6(7), P. 2533 - 2547

Published: May 24, 2024

Contemporary options for multidrug-resistant bacteria infected diabetic foot ulcers (IDFUs) are predominantly nonspecific. These IDFU injuries often display prolonged inflammation and delayed tissue repair, mainly attributed to an overabundance of M1 macrophages in the hostile microenvironment. Although immunomodulatory hydrogels show promise IDFU-focused care, a targeted, safe transition from M2 using simplified techniques remains significant obstacle. Here, we introduce hybrid hydrogel (GGG) with inherent capabilities IDFUs. GGG is composed interpenetrating polymer networks featuring gallium-induced self-assembling glycyrrhizic acid photo-cross-linked gelatin methacryloyl matrix. Importantly, effectively decreases ratio conditions drug-resistant by disrupting iron metabolism scavenging reactive oxygen species, which contributes enhanced treatment results wounds. To sum up, strategy present provides straightforward, safe, highly effective therapeutic avenue managing

Language: Английский

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Designing Single‐Atom Active Sites on sp²‐Carbon Linked Covalent Organic Frameworks to Induce Bacterial Ferroptosis‐Like for Robust Anti‐Infection Therapy

Advanced Science, Journal Year: 2023, Volume and Issue: 10(13)

Published: Feb. 27, 2023

With the threat posed by drug-resistant pathogenic bacteria, developing non-antibiotic strategies for eradicating clinically prevalent superbugs remains challenging. Ferroptosis is a newly discovered form of regulated cell death that can overcome drug resistance. Emerging evidence shows potential triggering ferroptosis-like antibacterial therapy, but direct delivery iron species inefficient and may cause detrimental effects. Herein, an effective strategy to induce bacterial nonferrous coordinating single-atom metal sites (e.g., Ir Ru) into sp

Language: Английский

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A Photo‐Therapeutic Nanocomposite with Bio‐Responsive Oxygen Self‐Supplying Combats Biofilm Infections and Inflammation from Drug‐Resistant Bacteria

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(37)

Published: May 25, 2023

Abstract The use of non‐antibiotic strategies to combat refractory drug‐resistant bacterial infections, especially biofilms and accompanying inflammation, has recently aroused widespread interest. Herein, a photo‐therapeutic nanocomposite with bio‐responsive oxygen (O 2 ) self‐supplying is introduced by integrating manganese dioxide (MnO nanozymes onto photosensitizer (indocyanine green, ICG)‐loaded mesoporous polydopamine nanoparticles (MPDA), namely MI‐MPDA NPs. can activate O generation in the infection microenvironment, thereby effectively alleviating biofilm hypoxia. Under near‐infrared light (NIR) irradiation, continuous supplying further boosts level singlet ( 1 ), enabling robust elimination through ‐potentiated photodynamic/photothermal therapy. Interestingly, down‐regulates factor expression inflammatory signaling pathways MnO ‐mediated reactive species scavenging, which ameliorates condition. Meanwhile, prevents M1‐phenotype switch macrophages from overexpression hypoxia‐inducible factor‐1 α (HIF‐1 prompting macrophage reprogramming toward pro‐regenerative M2‐phenotype. In mouse models subcutaneous implant‐associated caused methicillin‐resistant Staphylococcus aureus (MRSA) burn Pseudomonas aeruginosa biofilms, NIR‐irradiated not only eliminates formed but also alleviates oxidative stress drives cascade reaction immunomodulation‐wound healing. Overall, this strategy provides reliable tool for combating infections inflammation bacteria.

Language: Английский

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Environment‐Responsive Therapeutic Platforms for the Treatment of Implant Infection

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(26)

Published: May 15, 2023

Abstract The application of medical implants has greatly improved the survival rate and life quality patients. Nevertheless, in recent years, there are increasing cases implant dysfunction or failure because bacterial infections. Despite significant improvements biomedicine, still serious challenges treatment implant‐related With formation biofilms development resistance, these limitations lead to a low efficacy conventional antibiotics. To address challenges, it is urgent exploit innovative strategies for Based on ideas, environment‐responsive therapeutic platforms with high selectivity, drug minor dose‐limiting toxicity have attracted widespread attention. By using exogenous/endogenous stimuli, antibacterial activity therapeutics can be activated demand exhibit remarkable effects. Exogenous stimuli include photo, magnetism, microwave, ultrasound. Endogenous mainly pathological characteristics infections such as acidic pH, anomalous temperature, abnormal enzymatic activities. In this review, progress spatiotemporally controlled release/activation systematically summarized. Afterward, opportunities emerging highlighted. Finally, hoped that review will offer novel ideas techniques combat

Language: Английский

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Engineered Probiotic Bio‐heterojunction with Robust Antibiofilm Modality via “Eating” Extracellular Polymeric Substances for Wound Regeneration

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(35)

Published: June 25, 2024

The compact three-dimensional (3D) structure of extracellular polymeric substances (EPS) within biofilms significantly hinders the penetration antimicrobial agents, making biofilm eradication challenging and resulting in persistent biofilm-associated infections. To address this challenge, a solution is proposed: probiotic bio-heterojunction (P-bioHJ) combining Lactobacillus rhamnosus with MXene (Ti

Language: Английский

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Mechanochemically Reprogrammed Interface Orchestrates Neutrophil Bactericidal Activity and Apoptosis for Preventing Implant‐Associated Infection

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(16)

Published: Jan. 2, 2024

Abstract The onset of implant‐associated infection (IAI) triggers a cascade immune responses, which are initially dominated by neutrophils. Bacterial aggregate formation and hypoxic microenvironment, occur shortly after implantation, may be two major risk factors that impair neutrophil function lead to IAI. Here, the implant surface with phytic acid–Zn 2+ coordinated TiO 2 nanopillar arrays (PA–Zn@TiNPs) oxygen self‐supporting CaO nanoparticles, named as CPZTs, is mechanochemically reprogrammed. engineered CPZTs interface integrates multiple properties inhibit nascent biofilm, encompassing antibacterial adhesion, mechanobactericidal effect, chemobiocidal effect. Meanwhile, continuous oxygenation fuels neutrophils reactive species (ROS) for efficient bacterial elimination on inside Furthermore, this modulation strategy accelerates apoptosis promotes M2 macrophage‐mediated osteogenesis both in vitro rat model In conclusion, targeting immunomodulation practical effective prevent IAI promote bone–implant integration.

Language: Английский

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Honeycomb-inspired ZIF-sealed interface enhances osseointegration via anti-infection and osteoimmunomodulation

Biomaterials, Journal Year: 2024, Volume and Issue: 307, P. 122515 - 122515

Published: Feb. 21, 2024

Language: Английский

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Reactive Microneedle Patches with Antibacterial and Dead Bacteria‐Trapping Abilities for Skin Infection Treatment

Advanced Science, Journal Year: 2024, Volume and Issue: 11(23)

Published: April 6, 2024

Abstract Bacterial skin infections are highly prevalent and pose a significant public health threat. Current strategies primarily focused on the inhibition of bacterial activation while disregarding excessive inflammation induced by dead bacteria remaining in body effect acidic microenvironment during therapy. In this study, novel dual‐functional MgB 2 microparticles integrated microneedle (MgB MN) patch is presented to kill eliminate for infection management. The not only can produce local alkaline promote proliferation migration fibroblasts keratinocytes, but also achieve >5 log inactivation. Besides, effectively mitigate bacteria‐induced through interaction with lipopolysaccharide (LPS). With incorporation these microparticles, resultant MN patches capture bacteria, thereby mitigating inflammation. Therefore, show good therapeutic efficacy managing animal infections, including abscesses wounds. These results indicate that reactive metal borides‐integrated hold great promise treatment clinical infections.

Language: Английский

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