Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Oct. 28, 2024
Exploring
microorganisms
with
downstream
synthetic
advantages
in
lignin
valorization
is
an
effective
strategy
to
increase
target
product
diversity
and
yield.
This
study
ingeniously
engineers
the
non-lignin-degrading
bacterium
Ralstonia
eutropha
H16
(also
known
as
Cupriavidus
necator
H16)
convert
lignin,
a
typically
underutilized
by-product
of
biorefinery,
into
valuable
bioplastic
polyhydroxybutyrate
(PHB).
The
aromatic
metabolism
capacities
R.
for
different
lignin-derived
aromatics
(LDAs)
are
systematically
characterized
complemented
by
integrating
robust
functional
modules
including
O-demethylation,
aldehyde
mitigation
inhibition.
A
pivotal
discovery
regulatory
element
PcaQ,
which
highly
responsive
hub
metabolite
protocatechuic
acid
during
degradation.
Based
on
computer-aided
design
we
develop
metabolite-based
autoregulation
(HMA)
system.
system
can
control
genes
expression
response
heterologous
LDAs
enhance
efficiency.
Multi-module
genome
integration
directed
evolution
further
fortify
strain's
stability
conversion
capacities,
leading
PHB
production
titer
2.38
g/L
using
sole
carbon
source.
work
not
only
marks
leap
from
components
but
also
provides
redesign
non-LDAs-degrading
microbes
efficient
valorization.
One
challenge
that
possessing
metabolic
unable
lignin.
Here,
authors
engineer
efficiently
self-enhanced
Communications Biology,
Journal Year:
2025,
Volume and Issue:
8(1)
Published: Feb. 13, 2025
White-rot
fungi
are
efficient
organisms
for
the
mineralization
of
lignin
and
polysaccharides
into
CO2
H2O.
Despite
their
biotechnological
potential,
WRF
metabolism
remains
underexplored.
Building
on
recent
findings
regarding
utilization
lignin-related
aromatic
compounds
as
carbon
sources
by
WRF,
we
aimed
to
gain
further
insights
these
catabolic
processes.
For
this
purpose,
Trametes
versicolor
Gelatoporia
subvermispora
were
incubated
in
varying
conditions
–
static
agitation
modes
different
antioxidant
levels
during
conversion
4-hydroxybenzoic
acid
(a
compound)
cellobiose.
Their
metabolic
responses
assessed
via
transcriptomics,
proteomics,
lipidomics,
metabolomics,
microscopy
analyses.
These
analyses
reveal
significant
impact
cultivation
sugar
pathways,
well
lipid
composition
fungal
mycelia.
Additionally,
study
identifies
biosynthetic
pathways
production
extracellular
fatty
acids
phenylpropanoids
both
products
with
relevance
applications
provides
fate
nature.
Cultivation
white-rot
distinct
incubation
presence
a
compound
induces
changes
transcriptome,
proteome,
lipidome,
metabolome.
Green Chemistry,
Journal Year:
2024,
Volume and Issue:
26(11), P. 6760 - 6773
Published: Jan. 1, 2024
A
glucose–xylose–phenolics
(GXP)
system
was
developed
by
engineering
Escherichia
coli
–
consortia
to
achieve
the
full
use
of
lignocellulosic
biomass
for
efficient
synthesis
l
-tyrosine
and
its
analogues.
iScience,
Journal Year:
2025,
Volume and Issue:
28(2), P. 111771 - 111771
Published: Jan. 10, 2025
Polyphenol
oxidases
(PPOs)
are
coupled
binuclear
copper
proteins
that
catalyze
the
oxidation
of
phenols.
New
functions
PPOs
continuously
being
discovered,
latest
with
several
fungal
o-methoxy
phenolases,
which
active
on
lignin-derived
compounds.
Here,
we
perform
a
comprehensive
phylogenetic
analysis
from
wide
taxonomic
origin
and
define
12
PPO
groups.
We
find
deep
gene
duplication
has
led
to
two
distinct
types.
Type
1
includes
chordates
molluscs,
as
well
phenolases.
2
plant
PPOs,
molluscan
hemocyanins,
tyrosinases.
Most
type
have
C-terminal
shielding
domain
thioether
bond
in
copper-binding
site.
also
most
ascomycetes
contain
high
numbers
may
indicate
role
lignin
conversion
strategy
these
fungi.
