PLoS Genetics,
Journal Year:
2014,
Volume and Issue:
10(1), P. e1004047 - e1004047
Published: Jan. 2, 2014
Circadian
clocks
are
endogenous
oscillators
that
drive
the
rhythmic
expression
of
a
broad
array
genes,
orchestrating
metabolism
and
physiology.
Recent
evidence
indicates
post-transcriptional
post-translational
mechanisms
play
essential
roles
in
modulating
temporal
gene
for
proper
circadian
function,
particularly
molecular
mechanism
clock.
Due
to
technical
limitations
large-scale,
quantitative
protein
measurements,
it
remains
unresolved
what
extent
clock
regulates
by
driving
rhythms
abundance.
Therefore,
we
aimed
identify
global
oscillations
proteome
mouse
liver
applying
vivo
SILAC
technology
combination
with
state
art
mass
spectrometry.
Among
3000
proteins
accurately
quantified
across
two
consecutive
cycles,
6%
showed
defined
phase
expression.
Interestingly,
daily
one
fifth
were
not
accompanied
changes
at
transcript
level.
The
almost
half
cycling
delayed
more
than
six
hours
respect
corresponding,
mRNA.
Strikingly
observed
length
time
lag
between
mRNA
cycles
varies
day.
Our
analysis
revealed
high
coordination
abundance
involved
same
metabolic
process,
such
as
xenobiotic
detoxification.
Apart
from
specific
pathways,
identified
many
other
cellular
processes
which
levels
under
control,
instance
vesicle
trafficking
folding.
large-scale
proteomic
reveals
thus
key
role
orchestration
Proceedings of the National Academy of Sciences,
Journal Year:
2014,
Volume and Issue:
111(45), P. 16219 - 16224
Published: Oct. 27, 2014
Significance
We
generated
high-resolution
multiorgan
expression
data
showing
that
nearly
half
of
all
genes
in
the
mouse
genome
oscillate
with
circadian
rhythm
somewhere
body.
Such
widespread
transcriptional
oscillations
have
not
been
previously
reported
mammals.
Applying
pathway
analysis,
we
observed
new
clock-mediated
spatiotemporal
relationships.
Moreover,
found
a
majority
best-selling
drugs
United
States
target
gene
products.
Many
these
relatively
short
half-lives,
and
our
predict
which
may
benefit
from
timed
dosing.
The Journal of Experimental Medicine,
Journal Year:
2015,
Volume and Issue:
212(9), P. 1345 - 1360
Published: Aug. 10, 2015
Lymphocytes
must
adapt
to
a
wide
array
of
environmental
stressors
as
part
their
normal
development,
during
which
they
undergo
dramatic
metabolic
remodeling
process.
Research
in
this
area
has
yielded
surprising
findings
on
the
roles
diverse
pathways
and
metabolites,
have
been
found
regulate
lymphocyte
signaling
influence
differentiation,
function
fate.
In
review,
we
integrate
latest
field
provide
an
up-to-date
resource
metabolism.
Science,
Journal Year:
2016,
Volume and Issue:
354(6315), P. 1008 - 1015
Published: Nov. 24, 2016
A
majority
of
mammalian
genes
exhibit
daily
fluctuations
in
expression
levels,
making
circadian
rhythms
the
largest
known
regulatory
network
normal
physiology.
Cell-autonomous
clocks
interact
with
light-dark
and
feeding-fasting
cycles
to
generate
approximately
24-hour
oscillations
function
thousands
genes.
Circadian
secreted
molecules
signaling
components
transmits
timing
information
between
cells
tissues.
Such
intra-
intercellular
optimize
physiology
both
by
managing
energy
use
temporally
segregating
incompatible
processes.
Experimental
animal
models
epidemiological
data
indicate
that
chronic
rhythm
disruption
increases
risk
metabolic
diseases.
Conversely,
time-restricted
feeding,
which
imposes
feeding
fasting
without
caloric
reduction,
sustains
robust
diurnal
can
alleviate
These
findings
highlight
an
integrative
role
offer
a
new
perspective
for
treating
diseases
is
hallmark.
