Brucella
abortus
is
a
facultative
intracellular
pathogen
that
can
cause
chronic
persistent
infections
resulting
in
abortion
and
infertility
food
animals.
Recurrent
be
one
of
the
results
persister
cells
transiently
display
phenotypic
tolerance
to
high
dose
antibiotic
treatments.
We
examined
formation
B.
strain
A19
stationary
phase
investigated
potential
role
for
(p)ppGpp
synthetase
Rsh
this
process.
found
produce
higher
levels
multi-drug
tolerant
vitro
under
(20
×
MIC)
exposure
than
do
exponential
cells.
Persister
cell
was
also
induced
with
environmental
stressors
pH
4.5,
phosphate
buffer(pH7),
2
%
NaCl
25°C,
upon
ampicillin,
enrofloxacin
rifampicin.
were
not
formed
following
1
mM
H2O2.
The
numbers
significantly
increased
uptake
by
RAW264.7
cultured
macrophages.
Environmental
rsh
expression
mRNA
level.
null
mutants
(ΔRsh)
fewer
did
complemented
(CΔRsh)
wildtype
(WT)
strains
at
concentrations
rifampicin
vitro.
These
data
first
time
demonstrate
phase.
necessary
presence
On
basis,
new
understanding
recurrent
advanced,
thus
provided
basis
revelation
pathogenic
mechanism
infection
Brucella.
Signal Transduction and Targeted Therapy,
Journal Year:
2024,
Volume and Issue:
9(1)
Published: July 17, 2024
Abstract
Persisters
refer
to
genetically
drug
susceptible
quiescent
(non-growing
or
slow
growing)
bacteria
that
survive
in
stress
environments
such
as
antibiotic
exposure,
acidic
and
starvation
conditions.
These
cells
can
regrow
after
removal
remain
the
same
stress.
are
underlying
problems
of
treating
chronic
persistent
infections
relapse
treatment,
resistance
development,
biofilm
infections,
pose
significant
challenges
for
effective
treatments.
Understanding
characteristics
exact
mechanisms
persister
formation,
especially
key
molecules
affect
formation
survival
persisters
is
critical
more
treatment
infections.
Currently,
genes
related
being
discovered
confirmed,
but
by
which
form
very
complex,
there
still
many
unanswered
questions.
This
article
comprehensively
summarizes
historical
background
bacterial
persisters,
details
their
complex
relationship
with
tolerant
resistant
bacteria,
systematically
elucidates
interplay
between
various
biological
processes
cells,
well
consolidates
diverse
anti-persister
compounds
We
hope
provide
theoretical
in-depth
research
on
suggest
new
ideas
choosing
strategies
Proceedings of the National Academy of Sciences,
Journal Year:
2024,
Volume and Issue:
121(14)
Published: March 25, 2024
RNA
decay
is
a
crucial
mechanism
for
regulating
gene
expression
in
response
to
environmental
stresses.
In
bacteria,
RNA-binding
proteins
(RBPs)
are
known
be
involved
posttranscriptional
regulation,
but
their
global
impact
on
half-lives
has
not
been
extensively
studied.
To
shed
light
the
role
of
major
RBPs
ProQ
and
CspC/E
maintaining
stability,
we
performed
sequencing
Salmonella
enterica
over
time
course
following
treatment
with
transcription
initiation
inhibitor
rifampicin
(RIF-seq)
presence
absence
these
RBPs.
We
developed
hierarchical
Bayesian
model
that
corrects
confounding
factors
stability
assays
enables
us
identify
differentially
decaying
transcripts
transcriptome-wide.
Our
analysis
revealed
median
half-life
early
stationary
phase
less
than
1
min,
third
previous
estimates.
found
half
500
most
long-lived
bound
by
at
least
one
RBP,
suggesting
general
shaping
transcriptome.
Integrating
differential
estimates
cross-linking
immunoprecipitation
followed
(CLIP-seq)
approximately
30%
binding
sites
more
40%
coding
or
3′
untranslated
regions
respective
RBP.
Analysis
destabilized
identified
oxidative
stress
response.
findings
provide
insights
into
regulation
CspC/E,
importance
expression.
Bioscience Reports,
Journal Year:
2023,
Volume and Issue:
43(3)
Published: Feb. 14, 2023
RNA-binding
proteins
are
key
actors
of
post-transcriptional
networks.
Almost
exclusively
studied
in
the
light
their
interactions
with
RNA
ligands
and
associated
functional
events,
they
still
poorly
understood
as
evolutionary
units.
In
this
review,
we
discuss
FinO/ProQ
family
bacterial
chaperones,
how
evolve
spread
across
populations
what
properties
opportunities
provide
to
host
cells.
We
reflect
on
major
conserved
divergent
themes
within
family,
trying
understand
same
ancestral
fold,
augmented
additional
structural
elements,
could
yield
either
highly
specialised
or,
contrary,
globally
acting
regulatory
hubs
a
pervasive
impact
gene
expression.
also
consider
dominant
convergent
trends
that
shaped
chaperone
activity
recurrently
implicated
FinO/ProQ-like
DNA
metabolism,
translation
virulence.
Finally,
offer
new
perspective
which
FinO/ProQ-family
regulators
emerge
active
players
both
negative
positive
roles,
significantly
impacting
modes
trajectories
hosts.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: May 22, 2024
Persister
cells
are
transiently
tolerant
to
antibiotics
and
associated
with
recalcitrant
chronic
infections
due
recolonization
of
host
after
antibiotic
removal.
Brucella
spp.
facultative
pathogens
that
establish
intracellular
infection
cycles
in
which
results
persistent
infections.
abortus
forms
multi-drug
persister
promoted
by
the
(p)ppGpp
synthetase
Rsh
during
rifampicin
exposure.
Here,
we
confirmed
formation
B.
stationary
phase
treated
enrofloxacin.
Deletion
gene
for
decreased
level
presence
these
drugs
different
growth
phases.
However,
deletion
strain
varied
phases
other
antibiotics.
also
was
involved
treatment
under
certain
stress
conditions,
including
acidic
exposure
PBS,
heat
stress.
Moreover,
impacted
cell
levels
or
enrofloxacin
RAW264.7
macrophages.
Certain
typeIItoxin-antitoxin
modules
were
upregulated
various
conditions
.
We
established
positively
regulated
type
II
toxin-antitoxin
mbcTA
rifampicin-tolerant
elevated
ATP
when
promoter
overexpressed
background
phase.
Our
plays
a
key
role
persistence
may
serve
as
potent
novel
target
combination
development
new
therapeutic
approaches
prevention
strategies
treat
Nucleic Acids Research,
Journal Year:
2025,
Volume and Issue:
53(5)
Published: Feb. 27, 2025
The
RNA-binding
protein
ProQ
interacts
with
many
transcripts
in
the
bacterial
cell.
binding
is
associated
increased
messenger
RNA
(mRNA)
levels,
but
a
mechanistic
explanation
for
this
effect
has
been
lacking.
In
Salmonella
Typhimurium,
affects
key
traits
infection,
including
motility
and
intracellular
survival.
However,
direct
links
between
activity
these
phenotypes
are
not
well
understood.
Here,
we
demonstrate
that
promotes
biofilm
formation,
another
virulence-associated
phenotype.
This
strictly
dependent
on
sigma
factor
RpoS.
increases
both
RpoS
rpoS
mRNA
neither
transcription
nor
translation.
target,
expression
of
3'UTR
alone
strongly
ProQ.
becomes
independent
strains
lacking
poly(A)
polymerase
I
(PAPI),
indicating
protects
against
3'
end-dependent
decay.
Indeed,
purified
inhibits
PAPI-mediated
polyadenylation
at
ends.
Finally,
PAPI
required
ProQ's
genes
involved
biofilm,
motility,
osmotic
stress,
virulence,
inhibition
general
function
PLoS Pathogens,
Journal Year:
2025,
Volume and Issue:
21(5), P. e1013136 - e1013136
Published: May 14, 2025
Bacterial
persisters
contribute
significantly
to
clinical
treatment
failure
and
relapse.
These
cells
could
resist
antibiotic
via
transient
phenotypic
gene
expression
alterations.
We
conducted
a
high-throughput
screening
of
Salmonella
Typhimurium
transposon
mutants
identify
key
genes
for
intramacrophage
persistence.
The
results
show
that
sulfur
transferase
complex
encoded
by
yheM
,
yheL,
yheN
trmU
yhhP
are
involved
in
bacterial
persist
macrophages
downregulating
the
during
exposure
high
concentrations
antibiotics,
even
persistent
infection
mouse
model.
Mechanistically,
deletion
increases
reactive
nitrogen
species
(RNS)
exponential
phase,
which
inhibits
respiration
ATP
generation.
In
contrast,
absence
promotes
persister
formation
elevating
(p)ppGpp
levels
stationary
phase.
Taken
together,
our
data
demonstrate
bacteria
use
coordinate
replication
persistence
adaptation
various
environmental
stresses.
findings
reveal
role
provide
promising
target
antibacterial
therapy.
ACS Infectious Diseases,
Journal Year:
2024,
Volume and Issue:
10(8), P. 2525 - 2539
Published: June 28, 2024
The
ability
of
pathogenic
bacteria
to
evade
antibiotic
treatment
is
an
intricate
and
multifaceted
phenomenon.
Over
the
years,
failure
among
patients
due
determinants
antimicrobial
resistance
(AMR)
has
been
focal
point
for
research
development
new
therapeutic
agents.
However,
survival
by
persisting
under
stress
largely
overlooked.
Bacterial
persisters
are
a
subpopulation
sensitive
bacterial
cells
exhibiting
noninheritable
drug-tolerant
phenotype.
They
linked
recalcitrance
infections
in
healthcare
settings,
turn
giving
rise
AMR
variants.
importance
persistence
recurring
firmly
recognized.
Fundamental
work
over
past
decade
highlighted
numerous
unique
tolerance
factors
contributing
persister
phenotype
many
clinically
relevant
pathogens.
This
review
summarizes
that
could
aid
developing
strategies
against
persisters.
