Transcriptomic and genetic analysis reveals a Zn2Cys6 transcription factor specifically required for conidiation in submerged cultures of Thermothelomyces thermophilus DOI Creative Commons
Florian Drescher, Yang Li, José Manuel Villalobos‐Escobedo

et al.

mBio, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

ABSTRACT Filamentous fungi are important producers of enzymes and secondary metabolites. The industrial thermophilic species, Thermothelomyces thermophilus, is closely related to the model fungus, Neurospora crassa . A critical aspect filamentous fungal life cycle production asexual spores (conidia), which regulated by various stimuli, including nutrient availability. Several species fungi, T. thermophilus , produce conidia under submerged fermentation conditions, can be detrimental product yields. In this study, transcriptional profiling was used map changes during development in cultures, revealed commonalities regulation between N. We further identified a transcription factor, res1 whose deletion resulted complete loss but did not affect conidiation on plates. Under ∆ strain showed increased biomass relative wild-type strain, indicating that manipulation has potential increase productivity settings. Overexpression caused severe growth defect early both plates overexpression bypass regulatory aspects associated with Using chromatin-immunoprecipitation sequencing, we 35 target genes Res1, known regulators revealing common divergent reproduction these two species. IMPORTANCE such as have been harnessed Biotechnology industry for industrially relevant chemicals proteins. However, some will undergo switch from mycelial development. use identify factor specifically regulates developmental unwanted propagules thus altering secreted protein production. Mutations Res1 result cultures do sporulation when exposed air. identification stage-specific spore comparative analyses ascomycete manipulate advantage.

Language: Английский

Fungal infection of insects: molecular insights and prospects DOI Open Access
Hong Song, Junmei Shang,

Yaneli Sun

et al.

Trends in Microbiology, Journal Year: 2023, Volume and Issue: 32(3), P. 302 - 316

Published: Sept. 29, 2023

Language: Английский

Citations

37
Demography and Population Projection of Tetranychus urticae (Tetranychidae) on Phaseolus vulgaris (Fabaceae) Colonized by Entomopathogenic Fungal Endophytes DOI Creative Commons
Pengxiang Hong, Chandra Kanta Dash, Muhammad Adeel Ghafar

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(1), P. 73 - 73

Published: Jan. 21, 2024

Tetranychus urticae is a highly polyphagous and global pest. Spider mites primarily feed on the underside of leaves, resulting in decreased photosynthesis, nutritional loss, development chlorotic patches. We investigated life tables two-spotted spider mite T. fungal endophyte Beauveria bassiana colonized untreated plants common Phaseolus vulgaris L., bean plant. Based age-stage, two-sex table theory, data were evaluated. The raised had protonymphs, deutonymphs, total pre-adult stage durations that considerably shorter (1.76, 2.14, 9.77 d, respectively) than been (2.02, 2.45, 10.49 respectively). fecundity (F) varied from 28.01 eggs per female to 57.67 endophyte-untreated plants. net reproductive rate (R0) with without endophytes was 19.26 42.53 brood, respectively. an intrinsic increase (rm) 0.245 days as opposed plants, which r 0.196 finite (λ) (1.27 1.21, Population forecasts based two-sex, age-stage demonstrated dynamism variability structure. Furthermore, colonization B. negative impact growth urticae. It lowered adult span, fecundity, reproduction rate, rate. propose future research should better use entomopathogenic understand host plant resistance strategies integrated pest management.

Language: Английский

Citations

6
Current status and future trends of microbial and nematode-based biopesticides for biocontrol of crop pathogens DOI
Rayhane Hamrouni,

Flor Regus,

Anne‐Marie Farnet

et al.

Critical Reviews in Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 20

Published: July 10, 2024

The increasing public demand to avoid the use of synthetic pesticides and fertilizers in agricultural production systems, causing serious environmental damages, has challenged industry develop new effective solutions manage control phytopathogens. Biopesticides, particularly microbial-based biopesticides, are a promising alternative with high biodegradability, specificity, suitability for incorporation into integrated pest management practices, low likelihood resistance development, practically no known human health risks. However: expensive methods, narrow action spectra, susceptibility conditions, short shelf life, poor storage stability, legislation registry constraints, general lack knowledge slowing down their adoption. In addition regulatory framework revisions improved training initiatives, preservation thoughtfully designed formulations, field test validations needed offer microbial- nematode-based biopesticides efficacy increased shelf-life. During last several years, substantial advancements biopesticide have been developed. novelty part this review written 2023 is summarize (i) mechanisms beneficial microorganisms used increase crop performance (ii) successful formulation including commercial products biological phytopathogens based on microorganisms, nematode and/or metabolites.

Language: Английский

Citations

5
Subcellular biochemistry and biology of filamentous entomopathogenic fungi DOI
Sheng‐Hua Ying

Advances in applied microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 35 - 58

Published: Jan. 1, 2024

Language: Английский

Citations

3
Alcohol dehydrogenase 1 acts as a scaffold protein in mitophagy essential for fungal pathogen adaptation to hypoxic niches within hosts DOI
Jin‐Li Ding, Li Li, Kang Wei

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 295, P. 139651 - 139651

Published: Jan. 8, 2025

Language: Английский

Citations

0
Lipophagy acts as a nutritional adaptation mechanism for the filamentous entomopathogenic fungus Beauveria bassiana to colonize within the hosts DOI Creative Commons
Jin‐Li Ding, Ming‐Guang Feng, Sheng‐Hua Ying

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Metabolic adaptation to various nutrients is crucial for the pathogenic growth and virulence of filamentous fungal pathogens. Despite its importance, mechanisms underlying nutrient shifts, especially at subcellular level, remain incompletely understood. Our study aims investigate involved in metabolic fungi. The entomopathogenic fungus Beauveria bassiana was used as a representative Gene functional analyses were conducted via gene disruption complementation. Vacuolar targeting lipid droplets determined with transmission electron microscopy fluorescence microscopy. Protein interaction yeast-two hybridization co-immunoprecipitation methods. found initiate autophagy, further lipophagy, when transitioning from utilizing fatty acids carbohydrates, while also proliferating host hemocoel. three critical autophagy-related genes (ATG), specifically BbATG1, BbATG8, BbATG11, hindered vacuolar (LD) worsened impaired dimorphism acid medium subjected cell-wall perturbance stress. Notably, BbSun4, protein containing SUN4 domain, required it tagged droplets. BbMcp, which features methyl-accepting chemotaxis-like engaged directly both BbAtg8 thereby enhancing between these proteins. It important note that BbMcp solely facilitated lipophagy during shifts rather than starvation loss proved detrimental cytomembrane integrity, growth, overall development, ultimately leading marked reduction virulence. Lipophagy molecular pathway consists selective autophagy receptor, bridging factor, Atg8, essential colonizing within niches. This deepens our understanding mechanism fungus-host pathways autophagy.

Language: Английский

Citations

0
Multilayered regulation of autophagy-related protein kinase in Cordyceps militaris DOI
Yating Wang, Ying Wang, Bing Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 143523 - 143523

Published: May 3, 2025

Language: Английский

Citations

0
Mitochondrial and peroxisomal Lon proteases are functionally divergent in environmental adaptation and pathobiology of the entomopathogenic fungus Beauveria bassiana DOI
Hao Zhang,

Tian-Jing Chen,

Xinyi Wang

et al.

Journal of Invertebrate Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 108378 - 108378

Published: June 1, 2025

Language: Английский

Citations

0
Two aminopeptidase I homologs convergently contribute to pathobiology of fungal entomopathogen Beauveria bassiana via divergent physiology-dependent autophagy pathways for vacuolar targeting DOI Creative Commons
Jin‐Li Ding, Kang Wei, Ming‐Guang Feng

et al.

Journal of Advanced Research, Journal Year: 2023, Volume and Issue: 59, P. 1 - 17

Published: June 18, 2023

In yeast, the cytoplasm-to-vacuole targeting (Cvt) pathway acts as a biosynthetic autophagy-related process, in which vacuolar of hydrolase is mediated by machineries involved selective autophagy. However, mechanistic insights into hydrolases through autophagy still remain enigmatic filamentous fungi. Our study aims to investigate mechanisms The entomopathogenic fungus Beauveria bassiana was used representative We identified homologs yeast aminopeptidase I (Ape1) B. bioinformatic analyses and characterized their physiological roles gene function analyses. Pathways for were investigated via molecular trafficking has two are designated BbApe1A BbApe1B. Ape1 contribute starvation tolerance, development, virulence bassiana. Significantly, BbNbr1 receptor mediate proteins, BbApe1B interacts with also directly interacting BbAtg8, an additional requirement scaffold protein BbAtg11 that BbAtg8. Protein processing occurs at both terminuses only carboxyl terminus BbApe1B, dependent on proteins. Together, functions translocation processes proteins associated fungal lifecycle. This reveals insect-pathogenic fungi improves our understandings Nbr1-mediated

Language: Английский

Citations

6
Environmental persistence of the conidia of native entomopathogenic fungi and their efficiency on Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) DOI Open Access
Mehmet Sedat Sevinç, İsmail Karaca

Turkish Journal of Entomology, Journal Year: 2024, Volume and Issue: 48(3), P. 327 - 342

Published: Oct. 7, 2024

This study aimed to investigate the effects of local Beauveria bassiana (Balsamo) Vuillemin and Cordyceps fumosorosea (Wize) (Hypocreales: Cordycipitaceae) isolates on Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) prepupae, during 2020 2021 in Mediterranean Region Türkiye. In petri dishes, mortality rates varied with doses: for LD.2016 (B. bassiana), between 30 60%, M6-4 30-46%, IFR (C. fumosorosea), 65-100%. Additionally, differences were detected prepupa pupa periods larvae depending isolate. sterile soil studies, caused 53-93%, while those isolate 20-55%., doses. conidial survival 70-93% at 0 months 44-60% 12th month. The was among 37-55% months, decreased 2% reproductive capacity adult individuals that emerged alive after infection negatively affected time fungal semi-controlled field conditions, had 40 65%, 34 62%, It observed fungi used potential biological control C. capitata.

Language: Английский

Citations

2