Ecology of Endozoicomonadaceae in three coral genera across the Pacific Ocean

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: June 1, 2023

Abstract Health and resilience of the coral holobiont depend on diverse bacterial communities often dominated by key marine symbionts Endozoicomonadaceae family. The factors controlling their distribution functional diversity remain, however, poorly known. Here, we study ecology at an ocean basin-scale sampling specimens from three genera ( Pocillopora , Porites Millepora ) 99 reefs 32 islands across Pacific Ocean. analysis 2447 metabarcoding 270 metagenomic samples reveals that each genus harbored a distinct new species . These are composed nine lineages have biogeographic patterns. most common one, found in appears to be globally distributed symbiont with metabolic capabilities, including synthesis amino acids vitamins not produced host. other structured partly host genetic lineage mainly geographic location is more rarely associated Our results show different exhibit strategies host- associations defined bacteria level.

Language: Английский

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Horizon scanning the application of probiotics for wildlife

Trends in Microbiology, Journal Year: 2023, Volume and Issue: 32(3), P. 252 - 269

Published: Sept. 25, 2023

The provision of probiotics benefits the health a wide range organisms, from humans to animals and plants. Probiotics can enhance stress resilience endangered many which are critically threatened by anthropogenic impacts. use so-called 'probiotics for wildlife' is nascent application, field needs reflect on standards its development, testing, validation, risk assessment, deployment. Here, we identify main challenges this emerging intervention provide roadmap validate effectiveness wildlife probiotics. We cover essential inert negative controls in trials investigation probiotic mechanisms action. also suggest alternative microbial therapies that could be tested parallel with application. Our recommendations align approaches used humans, aquaculture, plants concept wildlife.

Language: Английский

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Beyond Restoration: Coral Microbiome Biotechnology

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 215 - 234

Published: Jan. 1, 2025

Language: Английский

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Microbial-Based Therapies to Restore and Rehabilitate Disrupted Coral Health

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 181 - 195

Published: Jan. 1, 2025

Language: Английский

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A role for bacterial experimental evolution in coral bleaching mitigation?

Trends in Microbiology, Journal Year: 2021, Volume and Issue: 30(3), P. 217 - 228

Published: Aug. 21, 2021

Language: Английский

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Host Traits and Phylogeny Contribute to Shaping Coral-Bacterial Symbioses

mSystems, Journal Year: 2022, Volume and Issue: 7(2)

Published: March 7, 2022

The success of tropical scleractinian corals depends on their ability to establish symbioses with microbial partners. Host phylogeny and traits are known shape the coral microbiome, but what extent they affect its composition remains unclear. Here, by using 12 species representing complex robust clades, we explored influence host phylogeny, skeletal architecture, reproductive mode microbiome composition, further investigated structure tissue skeleton bacterial communities. Our results show that explained 14% 13% providing evidence these predictors contributed shaping holobiont in terms presence relative abundance symbionts. Based our data, conclude affects specific lineages, predictably influences architecture works like a filter abundance. We β-diversity microbiomes differed, found large overlapping fraction sequences were recovered from both anatomical compartments, supporting hypothesis can function as reservoir. Additionally, analysis shows 99.6% 99.7% amplicon sequence variants (ASVs) not consistently present at least 30% samples, suggesting dominated rare bacteria. Together, provide novel insights into processes driving coral-bacterial symbioses, along an improved understanding microbiome.

Language: Английский

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Metagenomics-resolved genomics provides novel insights into chitin turnover, metabolic specialization, and niche partitioning in the octocoral microbiome

Microbiome, Journal Year: 2022, Volume and Issue: 10(1)

Published: Sept. 22, 2022

Abstract Background The role of bacterial symbionts that populate octocorals (Cnidaria, Octocorallia) is still poorly understood. To shed light on their metabolic capacities, we examined 66 high-quality metagenome-assembled genomes (MAGs) spanning 30 prokaryotic species, retrieved from microbial metagenomes three octocoral species and seawater. Results Symbionts healthy were affiliated with the taxa Endozoicomonadaceae , Candidatus Thioglobaceae Metamycoplasmataceae unclassified Pseudomonadales Rhodobacteraceae Alphaproteobacteria Ca. Rhabdochlamydiaceae . Phylogenomics inference revealed uncovered here represent two a novel genus unique to temperate octocorals, denoted Gorgonimonas eunicellae leptogorgiae Their capacities thrive under suboxic conditions high gene copy numbers serine-threonine protein kinases, type 3-secretion system, type-4 pili, ankyrin-repeat proteins, suggesting excellent capabilities colonize, aggregate, persist inside host. Contrarily, MAGs obtained seawater frequently lacked symbiosis-related genes. All harbored endo-chitinase chitin-binging protein-encoding genes, indicating they can hydrolyze most abundant polysaccharide in oceans. Other symbionts, including may assimilate smaller chitin oligosaccharides resulting breakdown engage deacetylation, respectively, possibilities for substrate cross-feeding coral microbiome overall turnover. We also observed sharp differences secondary metabolite production potential between symbiotic lineages. Specific Proteobacteria specialize chemical defense guard other which lack such capacity. Conclusion This first study recover dominant those so-far unculturable symbionts. identify thus-far unanticipated, global corals processing chitin, natural oceans major component zoo- phytoplankton feed octocorals. conclude niche partitioning, specialization, adaptation low oxygen among likely contribute plasticity adaptability holobiont changing marine environments. These findings bear implications not only our understanding relationships realm but functioning benthic ecosystems at large.

Language: Английский

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Harnessing the Power of Model Organisms To Unravel Microbial Functions in the Coral Holobiont

Microbiology and Molecular Biology Reviews, Journal Year: 2022, Volume and Issue: 86(4)

Published: Oct. 26, 2022

Stony corals build the framework of coral reefs, ecosystems immense ecological and economic importance. The existence these is threatened by climate change other anthropogenic stressors that manifest in microbial dysbiosis such as bleaching disease, often leading to mortality. Despite a significant amount research, mechanisms ultimately underlying destructive phenomena, what could prevent or mitigate them, remain be resolved. This mostly due practical challenges experimentation on highly complex nature holobiont also includes bacteria, archaea, protists, viruses. While overall importance partners well recognized, their specific contributions functioning interspecific dynamics largely unexplored. Here, we review potential adopting model organisms more tractable systems address knowledge gaps. We draw parallels from broader biological biomedical fields guide establishment, implementation, integration new emerging with aim addressing needs research. evaluate cnidarian models Hydra, Aiptasia, Cassiopea, Astrangia poculata; fast-evolving field tissue cell cultures; propose for establishment "true" tropical reef-building models. Based this assessment, suggest future research key aspects limiting our ability understand hence improve response ocean conditions.

Language: Английский

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Genomic exploration of coral-associated bacteria: identifying probiotic candidates to increase coral bleaching resilience in Galaxea fascicularis

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: Aug. 19, 2023

Reef-building corals are acutely threatened by ocean warming, calling for active interventions to reduce coral bleaching and mortality. Corals associate with a wide diversity of bacteria which can influence health, but knowledge specific functions that may be beneficial under thermal stress is scant. Under the oxidative theory bleaching, scavenge reactive oxygen (ROS) or nitrogen species (RNS) expected enhance resilience. Further, bacterial carbon export might substitute supply from algal photosymbionts, resilience facilitate recovery. To identify probiotic candidates, we sequenced genomes 82 pure-cultured were isolated emerging model Galaxea fascicularis.Genomic analyses showed isolates affiliated 37 genera. Isolates such as Ruegeria, Muricauda Roseovarius found encode genes synthesis antioxidants mannitol, glutathione, dimethylsulfide, dimethylsulfoniopropionate, zeaxanthin and/or β-carotene. Genes involved in RNS-scavenging many G. fascicularis-associated bacteria, represents novel finding several genera (including Pseudophaeobacter). Transporters suggested (semiSWEET) detected seven isolates, including Pseudovibrio Roseibium. range strains, strains Roseibium Roseovarius, revealed genomic features colonisation association host, secretion systems eukaryote-like repeat proteins.Our work provides an in-depth analysis functional potential identifies combinations traits coral's ability withstand bleaching. Identifying characterising critical development effective probiotics boost climate Video Abstract.

Language: Английский

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Exploring the Potential Molecular Mechanisms of Interactions between a Probiotic Consortium and Its Coral Host

mSystems, Journal Year: 2023, Volume and Issue: 8(1)

Published: Jan. 23, 2023

Probiotics are currently the main hope as a potential medicine for corals, organisms that considered marine “canaries of coal mine” and threatened with extinction. Our experiments have proved concept probiotics mitigate coral bleaching can also prevent mortality.

Language: Английский

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