Gut microbiome and clinical and lifestyle host factors associated with recurrent positive RT-PCR for SARS-CoV-2 DOI Creative Commons

Cristina Jiménez-Arroyo,

Natalia Molinero, Carlos Sabater

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Dec. 18, 2024

Background SARS-CoV-2 and COVID-19 are still active in the population. Some patients remained PCR-positive for more than 4 weeks, called “persistently PCR-positive”. Recent evidence suggests a link between gut microbiota susceptibility to COVID-19, although no studies have explored persistent PCR conditions. We aimed evaluate relationship positive RT-PCR, microbiome, individual host determinants. Methods A shotgun metagenomic analysis was conducted on fecal samples from 28 individuals affected by COVID-19. Patients were divided into two groups: those who had cleared virus within 30 days (designated as control group) (n = 15), beyond (called PCR+ 13). also investigated correlation prolonged viral clearance several additional factors, including clinical parameters, immune responses, microbial metabolites, dietary habits. Results The composition functionality of microbiome varied based duration positivity determined PCR. Compared group, group exhibited elevated pathogen levels augmented diversity functional gene families (p-value < 0.05). multi-omics integrating metagenomics, metadata revealed specific contribution certain blood markers this basophils, IgM, IgG (both general SARS-CoV-2), liver damage. Unhealthy diet identified significant factor influencing positivity. Conclusions These findings indicate that may play role delayed RT-PCR results. Our study contributes understanding factors mediators linking disease outcomes. Further large-scale must confirm these data; however, they suggest relevance monitoring changes early post-viral years providing healthcare support.

Language: Английский

The human gut microbiome and aging DOI Creative Commons
Evan S. Bradley, John P. Haran

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: June 3, 2024

The composition of the human gut microbiome has been observed to change over course an individual's life. From birth, it is shaped by mode delivery, diet, environmental exposures, geographic location, exposures medications, and aging itself. Here, we present a narrative review across lifespan with focus on its impacts age-related diseases in humans. We will describe how shaped, features that have associated at different phases life this can adversely affect healthy aging. Across lifespan, especially old age, diverse includes organisms suspected produce anti-inflammatory metabolites such as short-chain fatty acids, reported be These findings remarkably consistent regions world suggesting they could universal all cultures genetic backgrounds. Exactly these biologic processes thus promoting crucial targeting for interventions support health longevity.

Language: Английский

Citations

24
Exploring the vitamin biosynthesis landscape of the human gut microbiota DOI Creative Commons
Chiara Tarracchini, Gabriele Andrea Lugli, Leonardo Mancabelli

et al.

mSystems, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

ABSTRACT The human gut microbiota possesses the capacity to synthesize vitamins, especially B group which are recognized as indispensable for various biological processes both among members of these bacterial communities and host cells. Accordingly, vitamin production by intestinal commensals has attracted significant interest. Nevertheless, our current understanding synthesis is primarily based on individual genomic monoculture investigations, therefore not providing an overall view biosynthetic potential complex microbial communities. In study, we utilized over 100 genes known be involved in biosynthesis K vitamins assess corresponding approximately 8,000 microbiomes. Our analyses reveal that host-associated factors, such age geographical origin, appear influence diversity abundance pathways. Furthermore, identify substantially contribute functions at each stage life. Interestingly, inference co-associations network relationships uncovered apparent key role played folate cobalamin equilibrium establishment infant adult communities, respectively. IMPORTANCE Overall, this study expands microbe-mediated may provide novel targets improve availability essential micronutrients host.

Language: Английский

Citations

11
Early life microbial succession in the gut follows common patterns in humans across the globe DOI Creative Commons
Guilherme Fahur Bottino, Kevin S. Bonham, F. Patel

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 14, 2025

Language: Английский

Citations

1
Functional modulation of the human gut microbiome by bacteria vehicled by cheese DOI Creative Commons
Christian Milani,

Giulia Longhi,

Giulia Alessandri

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

ABSTRACT Since cheese is one of the most commonly and globally consumed fermented foods, scientific investigations in recent decades have focused on determining impact this dairy product human health well-being. However, modulatory effect exerted by autochthonous microbial community taxonomic composition associated functional potential gut microbiota still far from being fully dissected or understood. Here, through use an vitro gut-simulating cultivation model combination with multi-omics approaches, we shown that minor rather than dominant bacterial players are responsible for modulation consumers. These include taxa genera Enterococcus , Bacillus Clostridium, Hafnia . Indeed, they contribute to expand intestinal ecosystem introducing genes production metabolites relevant biological activity, including involved synthesis vitamins, short-chain fatty acids, amino acids. Furthermore, tracing microbiota-associated strains fecal samples consumers provided evidence horizontal transmission events, enabling detection particular transferred humans. Moreover, transcriptomic metabolomic analyses a horizontally transmitted (cheese-to-consumer) strain, i.e., paralvei T10, cultivated environment-simulating medium, confirmed concept cheese-derived bacteria may arsenal consumer’s microbiota. This highlights biologically contributions food microbes acquired consumption health. IMPORTANCE Diet universally recognized as primary factor influencing modulating both taxonomically functionally. In context, cheese, its own microbiota, serves not only source nourishment humans, but also nutrients Additionally, it act vehicle food-associated microorganisms which undergo transfer consumer, potentially host The current study can be traced repertoire significant implications

Language: Английский

Citations

1
Effects of bacteriophages on gut microbiome functionality DOI Creative Commons
Elena Kurilovich, Naama Geva‐Zatorsky

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: March 31, 2025

The gut microbiome, composed of bacteria, fungi, and viruses, plays a crucial role in maintaining the delicate balance human health. Emerging evidence suggests that microbiome disruptions can have far-reaching implications, ranging from development inflammatory diseases cancer to metabolic disorders. Bacteriophages, or "phages", are viruses specifically infect bacterial cells, their interactions with receiving increased attention. Despite recently revived interest phageome, it is still considered "dark matter" gut, more than 80% viral genomes remaining uncharacterized. Today, research focused on understanding mechanisms by which phages influence microbiota potential applications. Bacteriophages may regulate relative abundance communities, affect functions various ways, modulate mammalian host immunity. This review explores how functionality, particularly commensals pathogens, emphasizing health disease.

Language: Английский

Citations

1
System Overview from Physiological and Pathological Standpoints DOI
Gustavo Rodrigues Pedrino,

Luíza Toubas Chaul,

Virgínia Farias Alves

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Roadmap for alleviating the manifestations of ageing in the cardiovascular system DOI
Luca Liberale, Simon Tual‐Chalot, Simon Sedej

et al.

Nature Reviews Cardiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Language: Английский

Citations

0
Heart rate variability, daily cortisol indices and their association with psychometric characteristics and gut microbiota composition in an Italian community sample DOI Creative Commons

Sebastiano Ravenda,

Leonardo Mancabelli,

Sara Gambetta

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 12, 2025

Abstract The microbiota–gut–brain axis is a complex communication system that plays crucial role in influencing various aspects of our physical and mental health. goal this study was to determine the extent which individual differences resting measures vagally-mediated heart rate variability (HRV) cortisol levels were associated with psychometric specific gut microbiota characteristics seventy-five (38 females) healthy individuals. Participants assessed for HRV, daily salivary levels, characteristics, composition. Using categorical approach based on median split HRV values, we identified an association between low greater depressive symptomatology, altered (e.g., higher abundance Prevotella smaller Faecalibacterium , Alistipes Gemmiger). This suggests may be useful biomarker microbiota-gut brain function, play important bidirectional link dysbiosis depression. On other hand, parameters awakening response, diurnal slope) either anxiety perceived stress, or profile. Therefore, their utility as biomarkers microbiota-gut-brain function needs further scrutiny.

Language: Английский

Citations

0
Vitamin biosynthesis in the gut: interplay between mammalian host and its resident microbiota DOI
Chiara Tarracchini, Cathy Lordan, Christian Milani

et al.

Microbiology and Molecular Biology Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

SUMMARYIn recent years, exhaustive efforts have been made to dissect the composition of gut-associated microbial communities and associated interactions with their human host, which are thought play a crucial role in host development, physiology, metabolic functions. Although such studies were initially focused on description compositional shifts microbiota that occur between different health conditions, more recently, they provided key insights into functional contributions gut overall physiology. In this context, an important activity is believed be represented by synthesis various vitamins may elicit considerable benefits health. A growing body scientific literature now available relating (predicted) bacterial vitamin biosynthetic abilities, ever-growing information concerning prevalence these abilities among members microbiota. This review aimed at disentangling if how cooperative trophic contribute production, such, microbiota-mediated production varies according life stages. Moreover, it offers brief exploration diets influence shaping while also providing preliminary potential correlations microbiota-associated occurrence diseases and/or disorders.

Language: Английский

Citations

0
Synthetic vs. non-synthetic sweeteners: their differential effects on gut microbiome diversity and function DOI Creative Commons

Alex Kidangathazhe,

Theresah Amponsah,

Abhijit Maji

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 15, 2025

The rising use of artificial sweeteners, favored for their zero-calorie content and superior sweetness, necessitates understanding impact on the gut microbiome. This study examines effects five common sweeteners—Acesulfame K, Rebaudioside A, Saccharin, Sucralose, Xylitol—on microbiome diversity using minibioreactor arrays. Fecal samples from three healthy individuals were used to inoculate bioreactors that subsequently supplemented with each sweetener. Over 35 days, microbial network composition analyzed. Results revealed synthetic sweeteners like Sucralose Saccharin significantly reduced diversity, while non-synthetic particularly A Xylitol, less disruptive. Acesulfame K increased but disrupted structure, suggesting potential long-term negative impacts resilience. enriched pathogenic families such as Enterobacteriaceae, whereas natural promoted beneficial taxa Lachnospiraceae. Random Matrix Theory (RMT) based analysis highlighted distinct interaction patterns, causing persistent structural changes. Findings suggest may be more favorable health than ones, emphasizing cautious use, those concerns. enhances our sweeteners’ microbiome, highlighting need further research into implications.

Language: Английский

Citations

0