Frontiers in Cellular and Infection Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: Dec. 18, 2024
Background
SARS-CoV-2
and
COVID-19
are
still
active
in
the
population.
Some
patients
remained
PCR-positive
for
more
than
4
weeks,
called
“persistently
PCR-positive”.
Recent
evidence
suggests
a
link
between
gut
microbiota
susceptibility
to
COVID-19,
although
no
studies
have
explored
persistent
PCR
conditions.
We
aimed
evaluate
relationship
positive
RT-PCR,
microbiome,
individual
host
determinants.
Methods
A
shotgun
metagenomic
analysis
was
conducted
on
fecal
samples
from
28
individuals
affected
by
COVID-19.
Patients
were
divided
into
two
groups:
those
who
had
cleared
virus
within
30
days
(designated
as
control
group)
(n
=
15),
beyond
(called
PCR+
13).
also
investigated
correlation
prolonged
viral
clearance
several
additional
factors,
including
clinical
parameters,
immune
responses,
microbial
metabolites,
dietary
habits.
Results
The
composition
functionality
of
microbiome
varied
based
duration
positivity
determined
PCR.
Compared
group,
group
exhibited
elevated
pathogen
levels
augmented
diversity
functional
gene
families
(p-value
<
0.05).
multi-omics
integrating
metagenomics,
metadata
revealed
specific
contribution
certain
blood
markers
this
basophils,
IgM,
IgG
(both
general
SARS-CoV-2),
liver
damage.
Unhealthy
diet
identified
significant
factor
influencing
positivity.
Conclusions
These
findings
indicate
that
may
play
role
delayed
RT-PCR
results.
Our
study
contributes
understanding
factors
mediators
linking
disease
outcomes.
Further
large-scale
must
confirm
these
data;
however,
they
suggest
relevance
monitoring
changes
early
post-viral
years
providing
healthcare
support.
Gut Microbes,
Journal Year:
2024,
Volume and Issue:
16(1)
Published: June 3, 2024
The
composition
of
the
human
gut
microbiome
has
been
observed
to
change
over
course
an
individual's
life.
From
birth,
it
is
shaped
by
mode
delivery,
diet,
environmental
exposures,
geographic
location,
exposures
medications,
and
aging
itself.
Here,
we
present
a
narrative
review
across
lifespan
with
focus
on
its
impacts
age-related
diseases
in
humans.
We
will
describe
how
shaped,
features
that
have
associated
at
different
phases
life
this
can
adversely
affect
healthy
aging.
Across
lifespan,
especially
old
age,
diverse
includes
organisms
suspected
produce
anti-inflammatory
metabolites
such
as
short-chain
fatty
acids,
reported
be
These
findings
remarkably
consistent
regions
world
suggesting
they
could
universal
all
cultures
genetic
backgrounds.
Exactly
these
biologic
processes
thus
promoting
crucial
targeting
for
interventions
support
health
longevity.
mSystems,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 17, 2024
ABSTRACT
The
human
gut
microbiota
possesses
the
capacity
to
synthesize
vitamins,
especially
B
group
which
are
recognized
as
indispensable
for
various
biological
processes
both
among
members
of
these
bacterial
communities
and
host
cells.
Accordingly,
vitamin
production
by
intestinal
commensals
has
attracted
significant
interest.
Nevertheless,
our
current
understanding
synthesis
is
primarily
based
on
individual
genomic
monoculture
investigations,
therefore
not
providing
an
overall
view
biosynthetic
potential
complex
microbial
communities.
In
study,
we
utilized
over
100
genes
known
be
involved
in
biosynthesis
K
vitamins
assess
corresponding
approximately
8,000
microbiomes.
Our
analyses
reveal
that
host-associated
factors,
such
age
geographical
origin,
appear
influence
diversity
abundance
pathways.
Furthermore,
identify
substantially
contribute
functions
at
each
stage
life.
Interestingly,
inference
co-associations
network
relationships
uncovered
apparent
key
role
played
folate
cobalamin
equilibrium
establishment
infant
adult
communities,
respectively.
IMPORTANCE
Overall,
this
study
expands
microbe-mediated
may
provide
novel
targets
improve
availability
essential
micronutrients
host.
Applied and Environmental Microbiology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 28, 2025
ABSTRACT
Since
cheese
is
one
of
the
most
commonly
and
globally
consumed
fermented
foods,
scientific
investigations
in
recent
decades
have
focused
on
determining
impact
this
dairy
product
human
health
well-being.
However,
modulatory
effect
exerted
by
autochthonous
microbial
community
taxonomic
composition
associated
functional
potential
gut
microbiota
still
far
from
being
fully
dissected
or
understood.
Here,
through
use
an
vitro
gut-simulating
cultivation
model
combination
with
multi-omics
approaches,
we
shown
that
minor
rather
than
dominant
bacterial
players
are
responsible
for
modulation
consumers.
These
include
taxa
genera
Enterococcus
,
Bacillus
Clostridium,
Hafnia
.
Indeed,
they
contribute
to
expand
intestinal
ecosystem
introducing
genes
production
metabolites
relevant
biological
activity,
including
involved
synthesis
vitamins,
short-chain
fatty
acids,
amino
acids.
Furthermore,
tracing
microbiota-associated
strains
fecal
samples
consumers
provided
evidence
horizontal
transmission
events,
enabling
detection
particular
transferred
humans.
Moreover,
transcriptomic
metabolomic
analyses
a
horizontally
transmitted
(cheese-to-consumer)
strain,
i.e.,
paralvei
T10,
cultivated
environment-simulating
medium,
confirmed
concept
cheese-derived
bacteria
may
arsenal
consumer’s
microbiota.
This
highlights
biologically
contributions
food
microbes
acquired
consumption
health.
IMPORTANCE
Diet
universally
recognized
as
primary
factor
influencing
modulating
both
taxonomically
functionally.
In
context,
cheese,
its
own
microbiota,
serves
not
only
source
nourishment
humans,
but
also
nutrients
Additionally,
it
act
vehicle
food-associated
microorganisms
which
undergo
transfer
consumer,
potentially
host
The
current
study
can
be
traced
repertoire
significant
implications
Gut Microbes,
Journal Year:
2025,
Volume and Issue:
17(1)
Published: March 31, 2025
The
gut
microbiome,
composed
of
bacteria,
fungi,
and
viruses,
plays
a
crucial
role
in
maintaining
the
delicate
balance
human
health.
Emerging
evidence
suggests
that
microbiome
disruptions
can
have
far-reaching
implications,
ranging
from
development
inflammatory
diseases
cancer
to
metabolic
disorders.
Bacteriophages,
or
"phages",
are
viruses
specifically
infect
bacterial
cells,
their
interactions
with
receiving
increased
attention.
Despite
recently
revived
interest
phageome,
it
is
still
considered
"dark
matter"
gut,
more
than
80%
viral
genomes
remaining
uncharacterized.
Today,
research
focused
on
understanding
mechanisms
by
which
phages
influence
microbiota
potential
applications.
Bacteriophages
may
regulate
relative
abundance
communities,
affect
functions
various
ways,
modulate
mammalian
host
immunity.
This
review
explores
how
functionality,
particularly
commensals
pathogens,
emphasizing
health
disease.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: March 12, 2025
Abstract
The
microbiota–gut–brain
axis
is
a
complex
communication
system
that
plays
crucial
role
in
influencing
various
aspects
of
our
physical
and
mental
health.
goal
this
study
was
to
determine
the
extent
which
individual
differences
resting
measures
vagally-mediated
heart
rate
variability
(HRV)
cortisol
levels
were
associated
with
psychometric
specific
gut
microbiota
characteristics
seventy-five
(38
females)
healthy
individuals.
Participants
assessed
for
HRV,
daily
salivary
levels,
characteristics,
composition.
Using
categorical
approach
based
on
median
split
HRV
values,
we
identified
an
association
between
low
greater
depressive
symptomatology,
altered
(e.g.,
higher
abundance
Prevotella
smaller
Faecalibacterium
,
Alistipes
Gemmiger).
This
suggests
may
be
useful
biomarker
microbiota-gut
brain
function,
play
important
bidirectional
link
dysbiosis
depression.
On
other
hand,
parameters
awakening
response,
diurnal
slope)
either
anxiety
perceived
stress,
or
profile.
Therefore,
their
utility
as
biomarkers
microbiota-gut-brain
function
needs
further
scrutiny.
Microbiology and Molecular Biology Reviews,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 2, 2025
SUMMARYIn
recent
years,
exhaustive
efforts
have
been
made
to
dissect
the
composition
of
gut-associated
microbial
communities
and
associated
interactions
with
their
human
host,
which
are
thought
play
a
crucial
role
in
host
development,
physiology,
metabolic
functions.
Although
such
studies
were
initially
focused
on
description
compositional
shifts
microbiota
that
occur
between
different
health
conditions,
more
recently,
they
provided
key
insights
into
functional
contributions
gut
overall
physiology.
In
this
context,
an
important
activity
is
believed
be
represented
by
synthesis
various
vitamins
may
elicit
considerable
benefits
health.
A
growing
body
scientific
literature
now
available
relating
(predicted)
bacterial
vitamin
biosynthetic
abilities,
ever-growing
information
concerning
prevalence
these
abilities
among
members
microbiota.
This
review
aimed
at
disentangling
if
how
cooperative
trophic
contribute
production,
such,
microbiota-mediated
production
varies
according
life
stages.
Moreover,
it
offers
brief
exploration
diets
influence
shaping
while
also
providing
preliminary
potential
correlations
microbiota-associated
occurrence
diseases
and/or
disorders.
Frontiers in Microbiology,
Journal Year:
2025,
Volume and Issue:
16
Published: May 15, 2025
The
rising
use
of
artificial
sweeteners,
favored
for
their
zero-calorie
content
and
superior
sweetness,
necessitates
understanding
impact
on
the
gut
microbiome.
This
study
examines
effects
five
common
sweeteners—Acesulfame
K,
Rebaudioside
A,
Saccharin,
Sucralose,
Xylitol—on
microbiome
diversity
using
minibioreactor
arrays.
Fecal
samples
from
three
healthy
individuals
were
used
to
inoculate
bioreactors
that
subsequently
supplemented
with
each
sweetener.
Over
35
days,
microbial
network
composition
analyzed.
Results
revealed
synthetic
sweeteners
like
Sucralose
Saccharin
significantly
reduced
diversity,
while
non-synthetic
particularly
A
Xylitol,
less
disruptive.
Acesulfame
K
increased
but
disrupted
structure,
suggesting
potential
long-term
negative
impacts
resilience.
enriched
pathogenic
families
such
as
Enterobacteriaceae,
whereas
natural
promoted
beneficial
taxa
Lachnospiraceae.
Random
Matrix
Theory
(RMT)
based
analysis
highlighted
distinct
interaction
patterns,
causing
persistent
structural
changes.
Findings
suggest
may
be
more
favorable
health
than
ones,
emphasizing
cautious
use,
those
concerns.
enhances
our
sweeteners’
microbiome,
highlighting
need
further
research
into
implications.