Acoustofluidics for biomedical applications

Nature Reviews Methods Primers, Journal Year: 2022, Volume and Issue: 2(1)

Published: April 21, 2022

Language: Английский

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Extended topological valley-locked surface acoustic waves

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: March 14, 2022

Stable and efficient guided waves are essential for information transmission processing. Recently, topological valley-contrasting materials in condensed matter systems have been revealed as promising infrastructures guiding classical waves, they can provide broadband, non-dispersive reflection-free electromagnetic/mechanical wave transport with a high degree of freedom. In this work, by designing manufacturing miniaturized phononic crystals on semi-infinite substrate, we experimentally realized valley-locked edge surface acoustic (SAWs). Critically, original one-dimensional transports could be extended to quasi-two-dimensional ones doping SAW Dirac "semimetal" layers at the boundaries. We demonstrate that SAWs edges robust against bending wavelength-scaled defects. Also, mechanism is configurable depending doping, offering various on-chip manipulation, e.g., routing, focusing, splitting, converging, all flexible high-flow. This work may promote future hybrid circuits processing, sensing, manipulation.

Language: Английский

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Biomedical Applications of Microfluidic Devices: A Review

Biosensors, Journal Year: 2022, Volume and Issue: 12(11), P. 1023 - 1023

Published: Nov. 16, 2022

Both passive and active microfluidic chips are used in many biomedical chemical applications to support fluid mixing, particle manipulations, signal detection. Passive devices geometry-dependent, their uses rather limited. Active include sensors or detectors that transduce chemical, biological, physical changes into electrical optical signals. Also, they transduction detect biological applications, highly versatile tools for disease diagnosis organ modeling. This review provides a comprehensive overview of the significant advances have been made development microfluidics devices. We will discuss function as micromixers sorters cells substances (e.g., microfiltration, flow displacement, trapping). Microfluidic fabricated using range techniques, including molding, etching, three-dimensional printing, nanofabrication. Their broad utility lies detection diagnostic biomarkers organ-on-chip approaches permit modeling cancer, well neurological, cardiovascular, hepatic, pulmonary diseases. Biosensor allow point-of-care testing, assays based on enzymes, nanozymes, antibodies, nucleic acids (DNA RNA). An anticipated field includes optimization techniques fabrication biocompatible materials. These developments increase versatility, reduce costs, accelerate time technology.

Language: Английский

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A review on microfluidic-assisted nanoparticle synthesis, and their applications using multiscale simulation methods

Discover Nano, Journal Year: 2023, Volume and Issue: 18(1)

Published: Feb. 17, 2023

Abstract Recent years have witnessed an increased interest in the development of nanoparticles (NPs) owing to their potential use a wide variety biomedical applications, including drug delivery, imaging agents, gene therapy, and vaccines, where recently, lipid nanoparticle mRNA-based vaccines were developed prevent SARS-CoV-2 causing COVID-19. NPs typically fall into two broad categories: organic inorganic. Organic mainly include lipid-based polymer-based nanoparticles, such as liposomes, solid polymersomes, dendrimers, polymer micelles. Gold silver NPs, iron oxide quantum dots, carbon silica-based nanomaterials make up bulk inorganic NPs. These are prepared using top-down bottom-up approaches. Microfluidics provide attractive synthesis alternative is advantageous compared conventional methods. The microfluidic mixing-based production methods offer better control achieving desired size, morphology, shape, size distribution, surface properties synthesized technology also exhibits excellent process repeatability, fast handling, less sample usage, yields greater encapsulation efficiencies. In this article, we comprehensive review microfluidic-based passive active mixing techniques for NP synthesis, latest developments. Additionally, summary devices used presented. Nonetheless, despite significant advancements experimental procedures, complete details nanoparticle-based system cannot be deduced from experiments alone, thus, multiscale computer simulations utilized perform systematic investigations. work most common simulation unveiling critical mechanisms involved interaction with other entities, especially therapeutic systems. Finally, analysis provided on challenges microfluidics related future perspectives, large-scale hybrid formulations devices. Graphical abstract

Language: Английский

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Microscale acoustic streaming for biomedical and bioanalytical applications

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 160, P. 116958 - 116958

Published: Feb. 4, 2023

Language: Английский

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Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review

Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)

Published: June 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Language: Английский

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Acoustic separation and concentration of exosomes for nucleotide detection: ASCENDx

Science Advances, Journal Year: 2024, Volume and Issue: 10(10)

Published: March 8, 2024

Efficient isolation and analysis of exosomal biomarkers hold transformative potential in biomedical applications. However, current methods are prone to contamination require costly consumables, expensive equipment, skilled personnel. Here, we introduce an innovative spaceship-like disc that allows Acoustic Separation Concentration Exosomes Nucleotide Detection: ASCENDx. We created ASCENDx use acoustically driven rotation on a spinning droplet generate swift separation concentration exosomes from patient plasma samples. Integrated plasmonic nanostars the enable label-free detection enriched via surface-enhanced Raman scattering. Direct circulating microRNA samples by platform facilitated diagnostic assay for colorectal cancer with 95.8% sensitivity 100% specificity. overcomes existing limitations exosome-based molecular diagnostics holds powerful position future research, precision medicine, point-of-care medical diagnostics.

Language: Английский

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Topological acoustofluidics

Nature Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

The complex interaction of spin, valley and lattice degrees freedom allows natural materials to create exotic topological phenomena. interplay between wave hydrodynamics could offer promising opportunities for visualizing physics manipulating bioparticle unconventionally. Here we present acoustofluidic chips illustrate the elastic spin nonlinear fluid dynamics. We created streaming vortices chiral swirling patterns backward-immune particle transport. Using tracer particles, observed arrays clockwise anticlockwise due an increase in density. Moreover, discovered pressure wells fluids, creating nanoscale trapping fields DNA molecules. also found a 93.2% modulation bandwidth edge states, dependent on orientation substrate's crystallographic structure. Our study sets stage uncovering phenomena revealing potential topological-material applications life sciences. authors valley-Hall chip interactions dynamics, its towards on-chip biological applications.

Language: Английский

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Label-Free Isolation of Exosomes Using Microfluidic Technologies

ACS Nano, Journal Year: 2021, Volume and Issue: 15(11), P. 17047 - 17079

Published: Nov. 1, 2021

Exosomes are cell-derived structures packaged with lipids, proteins, and nucleic acids. They exist in diverse bodily fluids involved physiological pathological processes. Although their potential for clinical application as diagnostic therapeutic tools has been revealed, a huge bottleneck impeding the development of applications rapidly burgeoning field exosome research is an inability to efficiently isolate pure exosomes from other unwanted components present fluids. To date, several approaches have proposed investigated separation, leading candidate being microfluidic technology due its relative simplicity, cost-effectiveness, precise fast processing at microscale, amenability automation. Notably, avoiding need labeling represents significant advance terms process time, cost well protecting biological activities exosomes. Despite exciting progress strategies isolation countless benefits label-free applications, current platforms still facing series problems challenges that prevent use sample processing. This review focuses on recent developed including those based sieving, deterministic lateral displacement, flow, pinched flow fractionation viscoelastic, acoustic, inertial, electrical, centrifugal forces. Further, we discuss advantages disadvantages these highlights outlook microfluidics toward utility

Language: Английский

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Multiphysics microfluidics for cell manipulation and separation: a review

Lab on a Chip, Journal Year: 2021, Volume and Issue: 22(3), P. 423 - 444

Published: Nov. 19, 2021

Multiphysics microfluidics, which combines multiple functional physical processes in a microfluidics platform, is an emerging research area that has attracted increasing interest for diverse biomedical applications. expected to overcome the limitations of individual phenomena through combining their advantages. Furthermore, multiphysics superior cell manipulation due its high precision, better sensitivity, real-time tunability, and multi-target sorting capabilities. These exciting features motivate us review this state-of-the-art field reassess feasibility coupling processes. To confine scope paper, we mainly focus on five common forces microfluidics: inertial lift, elastic, dielectrophoresis (DEP), magnetophoresis (MP), acoustic forces. This first explains working mechanisms single phenomena. Next, classify techniques terms cascaded connections coupling, elaborate combinations designs systems reported literature date. Finally, discuss possibility associated design schemes propose several promising future directions.

Language: Английский

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