MzmL, a novel marine derived N-acyl homoserine lactonase from Mesoflavibacter zeaxanthinifaciens that attenuates Pectobacterium carotovorum subsp. carotovorum virulence DOI Creative Commons
Lingyun Hao,

Jinyou Liang,

Shuotian Chen

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 9, 2024

Quorum sensing (QS) is a conserved cell–cell communication mechanism widely distributed in bacteria, and oftentimes tightly correlated with pathogen virulence. quenching enzymes, which interfere QS through degrading the signaling molecules, could attenuate virulence instead of killing pathogens, thus are less likely to induce drug resistance. Many Gram-negative bacteria produce N -acyl homoserine lactones (AHLs) for interspecies communication. In this study, we isolated identified bacterial strain, Mesoflavibacter zeaxanthinifaciens XY-85, from an Onchidium sp. collected intertidal zone Dapeng Reserve Shenzhen, China, found it had strong AHL degradative activity. Whole genome sequencing blast analysis revealed that XY-85 harbors lactonase (designated MzmL), predicted have -terminal signal peptide share “HXHXDH” motif known lactonases belonging Metallo- β -lactamase superfamily. Phylogenetic studies showed MzmL was closest marine cluster members, MomL Aii20J, AiiA type lactonases. Ultra performance liquid chromatography-mass spectrometry confirmed functions as catalyzing degradation lactone hydrolysis. degrade both short- long-chain AHLs or without substitution oxo-group at C-3 position, retained full bioactivity under wide range temperatures (28–100°C) pHs (4–11). Furthermore, significantly reduced Pectobacterium carotovorum subsp. factor production vitro , such biofilm formation plant cell wall enzyme production, inhibited soft rot development on potato slices. These results demonstrated may be novel good environmental stability, has great potential developed into biological control agent disease management.

Language: Английский

Fungal Endophytes as Mitigators against Biotic and Abiotic Stresses in Crop Plants DOI Creative Commons

Hittanahallikoppal Gajendramurthy Gowtham,

P. Hema,

M. Murali

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(2), P. 116 - 116

Published: Jan. 30, 2024

The escalating global food demand driven by a gradually expanding human population necessitates strategies to improve agricultural productivity favorably and mitigate crop yield loss caused various stressors (biotic abiotic). Biotic stresses are phytopathogens, pests, nematodes, along with abiotic like salt, heat, drought, heavy metals, which pose serious risks security productivity. Presently, the traditional methods relying on synthetic chemicals have led ecological damage through unintended impacts non-target organisms emergence of microbes that resistant them. Therefore, addressing these challenges is essential for economic, environmental, public health concerns. present review supports sustainable alternatives, emphasizing possible application fungal endophytes as innovative eco-friendly tools in plant stress management. Fungal demonstrate capabilities managing plants against biotic via direct or indirect enhancement plants’ innate immunity. Moreover, they contribute elevated photosynthesis rates, stimulate growth, facilitate nutrient mineralization, produce bioactive compounds, hormones, enzymes, ultimately improving overall resistance. In conclusion, harnessing potentiality represents promising approach toward sustainability practices, offering effective alternative solutions reduce reliance chemical treatments address posed stresses. This ensures long-term promotes environmental economic viability agriculture.

Language: Английский

Citations

27
Microorganisms in biological control strategies to manage microbial plant pathogens: a review DOI

Manswama Boro,

Shuvankar Sannyasi,

Dixita Chettri

et al.

Archives of Microbiology, Journal Year: 2022, Volume and Issue: 204(11)

Published: Oct. 10, 2022

Language: Английский

Citations

59
Ralstonia solanacearum: An Arsenal of Virulence Strategies and Prospects for Resistance DOI Creative Commons
Fabienne Vailleau, Stéphane Genin

Annual Review of Phytopathology, Journal Year: 2023, Volume and Issue: 61(1), P. 25 - 47

Published: July 28, 2023

The group of strains constituting the Ralstonia solanacearum species complex (RSSC) is a prominent model for study plant-pathogenic bacteria because its impact on agriculture, owing to wide host range, worldwide distribution, and long persistence in environment. RSSC have led numerous studies aimed at deciphering molecular bases virulence, many biological functions mechanisms been described contribute infection pathogenesis. In this review, we put into perspective recent advances our understanding virulence strains, both terms inventory that participate process their evolutionary dynamics. We also present different strategies developed combat these pathogenic through control, antimicrobial agents, plant genetics, or microbiota engineering.

Language: Английский

Citations

37
Functional Peptides for Plant Disease Control DOI Creative Commons
Emilio Montesinos

Annual Review of Phytopathology, Journal Year: 2023, Volume and Issue: 61(1), P. 301 - 324

Published: June 2, 2023

Plant disease control requires novel approaches to mitigate the spread of and losses caused by current, emerging, re-emerging diseases adapt plant protection global climate change restrictions on use conventional pesticides. Currently, management relies mainly biopesticides, which are required for sustainable plant-protection products. Functional peptides candidate biopesticides because they originate from living organisms or synthetic analogs provide mechanisms action against pathogens. Hundreds compounds exist that cover an extensive range activities viruses, bacteria phytoplasmas, fungi oomycetes, nematodes. Natural sources, chemical synthesis, biotechnological platforms may at large scale industry growers. The main challenges their in (a) requirement stability environment counteracting resistance pathogen populations, (b) need develop suitable formulations increase shelf life methods application, (c) selection with acceptable toxicological profiles, (d) high cost production agricultural purposes. In near future, it is expected several functional will be commercially available control, but more effort needed validate efficacy field level fulfill requirements regulatory framework.

Language: Английский

Citations

25
Discovery and Mechanism of Azatryptanthrin Derivatives as Novel Anti-Phytopathogenic Bacterial Agents for Potent Bactericide Candidates DOI

Guanglong Zhang,

Cheng‐Peng Li,

Yan Li

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(16), P. 6288 - 6300

Published: April 11, 2023

The natural alkaloids of tryptanthrin and their derivatives have a wide range biological activities. In this research, four series azatryptanthrin containing 4-aza/3-aza/2-aza/1-aza were prepared by condensation cyclization reaction against plant pathogens to develop new product-based bacterial pesticide. Compound 4Aza-8 displayed remarkable growth inhibitory effect on pathogenic bacteria Xanthomonas axonopodis pv. citri (Xac), oryzae Oryzae (Xoo), Pseudomonas syringae actinidiae (Psa) with the final corrected EC50 values 0.312, 1.91, 18.0 μg/mL, respectively, which greatly superior than that (Tryp). Moreover, also showed effective therapeutic protective activities in vivo citrus canker. Further mechanism studies Xac elucidated compound was able affect curve formation biofilm, cause severe shrinkage morphology, increase reactive oxygen species levels, induce apoptosis cells. Quantitative analysis differential protein profiles found major differences mainly concentrated endometrial secretion system pathway, blocked membrane transport affected transfer DNA host cell. summary, these research results suggest represents promising anti-phytopathogenic-bacteria agent, is worth being further investigated as bactericide candidate.

Language: Английский

Citations

20
Phages enhance both phytopathogen density control and rhizosphere microbiome suppressiveness DOI Creative Commons
Xiaofang Wang, Shuo Wang,

Mingcong Huang

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(6)

Published: May 23, 2024

Bacteriophages, viruses that specifically target plant pathogenic bacteria, have emerged as a promising alternative to traditional agrochemicals. However, it remains unclear how phages should be applied achieve efficient pathogen biocontrol and what extent their efficacy is shaped by indirect interactions with the resident microbiota. Here, we tested if phage of

Language: Английский

Citations

7
Current trends in management of bacterial pathogens infecting plants DOI
Aditi Sharma, Anupama Gupta,

Banita Devi

et al.

Antonie van Leeuwenhoek, Journal Year: 2023, Volume and Issue: 116(4), P. 303 - 326

Published: Jan. 23, 2023

Language: Английский

Citations

13
Silver nanoclusters with Ag2+/3+ oxidative states are a new highly effective tool against phytopathogenic bacteria DOI Creative Commons
Benedetta Orfei, Chiaraluce Moretti, Stefania Loreti

et al.

Applied Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 107(14), P. 4519 - 4531

Published: June 8, 2023

The main measure worldwide adopted to manage plant bacterial diseases is based on the application of copper compounds, which are often partially efficacious for frequent appearance copper-resistant strains and have raised concerns their toxicity environment humans. Therefore, there an increasing need develop new environmentally friendly, efficient, reliable strategies controlling diseases, among them, use nanoparticles seems promising. present study aimed evaluate feasibility protecting plants against attacks gram-negative gram-positive phytopathogenic bacteria by using electrochemically synthesized silver ultra nanoclusters (ARGIRIUM‑SUNCs®) with average size 1.79 nm characterized rare oxidative states (Ag2+/3+). ARGIRIUM‑SUNCs strongly inhibited in vitro growth (effective concentration, EC50, less than 1 ppm) biofilm formation Pseudomonas syringae pv. tomato quarantine Xanthomonas vesicatoria, Xylella fastidiosa subsp. pauca, Clavibacter michiganensis michiganensis. In addition, treatments also provoked eradication P. tomato, X. C. Treatment via root absorption (10 not phytotoxic protected (80%) attacks. at low doses induced hormetic effects as well growth. a possible alternative control measure. KEY POINTS: • ARGIRIUM‑SUNC has strong antimicrobial activities bacteria; inhibits doses; protects speck disease.

Language: Английский

Citations

12
Biological synthesis of Ag-nanoparticles using Stachys parviflora and its inhibitory potential against Xanthomonas campestris DOI
Shazia Dilbar, Hassan Sher, Ahmad Ali

et al.

South African Journal of Botany, Journal Year: 2023, Volume and Issue: 157, P. 409 - 422

Published: April 15, 2023

Language: Английский

Citations

11
Erucamide: A special biological control agent targeting T3SS for plant bacterial diseases DOI Creative Commons

Longzhu Bao,

Chang Min, Shaoyong Ke

et al.

Advanced Agrochem, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0