Regenerative Medicine, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19
Published: Dec. 17, 2024
Extracellular Vesicles (EVs) became a focus of clinical research when experimental and pre-clinical studies showed that they mimic their parent cells' regenerative therapeutic effects cargo carries disease-specific diagnostic prognostic biomarkers. Since the publication data forms an endpoint study, this review specifically focused on
Language: Английский
EMBO Reports, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 31, 2024
Language: Английский
Citations
6
Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(8)
Published: Aug. 1, 2024
Abstract The benefits of regular physical exercise on cancer prevention, as well reducing fatigue, treatment side effects and recurrence, improving quality life overall survival patients, are increasingly recognised. Initial studies showed that the concentration extracellular vesicles (EVs) increases during activity EVs carry biologically active cargo. These released by blood cells, skeletal muscle other organs involved in exercise, thus suggesting may mediate tissue crosstalk exercise. This possibility triggered a great interest study roles systemic adaptation to their potential applications prevention various diseases, including cancer. review presents exploring molecular cargo Furthermore, we discuss putative stimuli trigger EV release from cell types, biological functions impact exercise‐induced development progression. Understanding interplay between EVs, biology offer insights into novel therapeutic strategies preventive measures for
Language: Английский
Citations
5
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: March 27, 2024
Abstract Molecular control of recovery after exercise in muscle is temporally dynamic. A time course biopsies around resistance (RE) combined with -omics necessary to better comprehend the molecular contributions skeletal adaptation humans. Vastus lateralis before and 30 minutes, 3-, 8-, 24-hours acute RE were collected. time-point matched biopsy-only group was also included. RNA-sequencing defined transcriptome while DNA methylomics computational approaches complemented these data. The post-RE revealed: 1) methylome responses at minutes corresponded upregulated genes 3 hours, 2) a burst translation- transcription-initiation factor-coding transcripts occurred between 8 3) global gene expression peaked 4) ribosome-related dominated mRNA landscape 24 5) methylation-regulated MYC highly influential transcription factor throughout 24-hour played primary role levels hours. influence human strengthened by information from overexpression mouse muscle. To test whether sufficient for hypertrophy, we generated fiber-specific doxycycline inducible model pulsatile induction. Periodic 48-hour pulses over 4 weeks resulted higher mass fiber size soleus adult female mice. Collectively, present resolved resource understanding adaptations reveal as regulator RE-induced hypertrophy.
Language: Английский
Citations
4
Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(3)
Published: Jan. 13, 2025
Exercising regularly promotes health, but these benefits are complicated by acute inflammation induced exercise. A potential source of is cell-free DNA (cfDNA), yet the cellular origins, molecular causes, and immune system interactions exercise-induced cfDNA unclear. To study these, 10 healthy individuals were randomized to a 12-wk exercise program either high-intensity tactical training (HITT) or traditional moderate-intensity (TRAD). Blood plasma was collected pre- postexercise at weeks 0 12 after 4 wk detraining upon completion. Whole-genome enzymatic methylation sequencing (EM-seq) with cell-type proportion deconvolution applied obtained from 50 samples paired concentration measurements for 90 circulating cytokines. Acute increased release neutrophils, dendritic cells (DCs), macrophages proportional intensity. Exercise reduced released in HITT participants not TRAD DCs neutrophils. For most participants, lowered mitochondrial rest, even detraining. Using analysis approach we developed, concluded that rapid ETosis, process cell death where extracellular traps, likely cfDNA, demonstrated enrichment nuclear DNA. Further, several cytokines exercise, such as IL-6, IL-10, IL-16, attenuated induction only IL-6 IL-17F. Cytokine levels associated induction, suggesting main cause cfDNA. Overall, intensity modulated cytokine responses, contributing anti-inflammatory effects regular
Language: Английский
Citations
0
Journal of sport and health science/Journal of Sport and Health Science, Journal Year: 2025, Volume and Issue: unknown, P. 101029 - 101029
Published: Feb. 1, 2025
Advances in skeletal muscle omics has expanded our understanding of exercise-induced adaptations at the molecular level. Over past 2 decades, transcriptome studies have detailed acute and chronic responses to resistance, endurance, concurrent exercise, focusing on variables such as training status, nutrition, age, sex, metabolic health profile. Multi-omics approaches, integration transcriptomic epigenetic data, along with emerging ribosomal RNA sequencing advancements, further provided insights into how adapts exercise across lifespan. Downstream transcriptome, proteomic phosphoproteomic identified novel regulators adaptations, while single-cell/nucleus spatial technologies promise evolve cellular specialization communication around cells. This narrative review highlights (a) historical foundations muscle, (b) current research 3 layers cascade (DNA, RNA, protein), (c) applications single-cell study adaptation exercise. Further elaboration muscle's global footprint using multi-omics methods will help researchers practitioners develop more effective targeted approaches improve well athletic performance.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: March 11, 2025
Low-carbohydrate, high-fat diets enhance lipid metabolism and decrease reliance on glucose oxidation in athletes, but the associated gene expression patterns remain unclear. The purpose of this study was to determine whether coordinated molecular pathways skeletal muscle may be revealed by differential genes driven dietary profile, exercise, and/or their interaction. We investigated transcriptome elite ultra-endurance athletes habitually (~ 20 months) consuming a high-carbohydrate, low-fat (HC, n = 10, 33 ± 6y, VO2max 63.4 6.2 mL O2•kg-1•min-1) or low-carbohydrate, (LC, 34 7y, 64.7 3.7 diet. Skeletal measured at baseline (BL), immediately-post (H0), 2 h (H2) after 3 submaximal treadmill running. Diet induced divergent pattern BL where LC had higher with metabolism. Exercise resulted dynamic uniform response, no major differences between groups (H0). At H2, were pathway activity, including inflammation/immunity, suggesting diet-specific influence early recovery. These results indicate that lead resting exercise-induced patterns, underlying our previous findings fuel utilization athletes.
Language: Английский
Citations
0
Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111852 - 111852
Published: May 1, 2025
Language: Английский
Citations
0
The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(22)
Published: Nov. 15, 2024
Abstract Resistance exercise upregulates and downregulates the expression of a wide range genes in skeletal muscle. However, detailed analysis mRNA dynamics such as response rates temporal patterns transcriptome after resistance has not been performed. We aimed to clarify time‐series transcriptomics exercise. used electrical stimulation‐induced muscle contraction model (5 sets × 10 times 3 s 100‐Hz stimulation) on tibialis anterior rats measured before at 0, 1, 3, 6, 12 h contractions by RNA sequencing. also examined relationship between parameters increase protein contractions. found that function upregulated differed depending their rate. Genes related differentiation mechanical stimulus were enriched sustainedly genes. Furthermore, there was positive correlation magnitude corresponding level Although it theoretically suggested, this study experimentally demonstrated contributes adaptation via increases expression. These findings suggest rate, sustained pattern, contribute mechanisms underlying
Language: Английский
Citations
2
Journal of Cachexia Sarcopenia and Muscle, Journal Year: 2023, Volume and Issue: 14(6), P. 2925 - 2938
Published: Nov. 20, 2023
Abstract Background Exercise mimetics is a proposed class of therapeutics that specifically mimics or enhances the therapeutic effects exercise. Muscle glycogen and lactate extrusion are critical for physical performance. The mechanism by which metabolism manipulated during exercise remains unclear. This study aimed to assess effect miR‐92b on upregulation training‐induced Methods Adeno‐associated virus (AAV)‐mediated skeletal muscle overexpression in C57BLKS/J mice, global knockout mice were used explore function muscle. AAV‐mediated UGP2 MCT4 knockdown WT miR‐92 was confirm whether regulates through MCT4. Body weight, grip strength, running time distance exhaustion, histology assessed. expression levels mass‐related function‐related proteins analysed immunoblotting immunostaining. Results Global resulted normal body weight insulin sensitivity, but higher content before exhaustion (0.8538 ± 0.0417 vs. 1.043 0.040, ** P = 0.0087), lower after (4.133 0.2589 3.207 0.2511, * 0.0279), better capacity (running 3616 86.71 4231 90.29, *** 0.0006; 186.8 8.027 220.8 3.156, 0.0028), as compared with those observed control mice. Mice overexpressing (both miR‐92b‐3p miR‐92b‐5p) displayed (0.6318 0.0231 0.535 0.0194, 0.0094), accumulation (4.5 0.2394 5.467 0.1892, 0.01), poorer 4005 81.65 3228 149.8, <0.0001; 225.5 7.689 163 6.476, 0.001). Mechanistic analysis revealed targets UDP‐glucose pyrophosphorylase 2 (UGP2) inhibit synthesis, while miR‐92b‐5p represses directly target monocarboxylate transporter 4 (MCT4). Knockdown reversed absence vivo. Conclusions regulatory pathways, including miR‐92b‐3p/UGP2/glycogen synthesis miR‐92b‐5p/MCT4/lactate extrusion, could be targeted capacity.
Language: Английский
Citations
4
Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 719, P. 150075 - 150075
Published: May 10, 2024
Abundant evidence has shown the protective effect of aerobic exercise on central neuronal system, however, research about resistance remains limited. To evaluate and potential molecular mechanisms in improving cognition mental health, three-month-old male C57BL/6J mice underwent training for five weeks. Body parameters, cognitive performance synaptic plasticity were then assessed. In both groups, total RNA from frontal cortex, hippocampus gastrocnemius was isolated sequenced, GO term KEGG analysis performed to identify mechanisms. The results sequencing verified by RT-PCR. Our data found that group showed reduced anxiety-like behavior better spatial memory. Accordingly, specifically increased number thin spines without affecting other kind spines. mRNA sequence induced differential expression hundreds genes above three tissues. indicated FoxO signaling pathway most significant changed throughout brain muscle. terms Sgk1 enriched key related BP, including long-term memory, learning or memory level positive with water maze. conclusion, improved mice. Integrating profiles muscle reveals as mediator brain-muscle crosstalk.
Language: Английский
Citations
1