Cell types and cell circuits in human and non-human primate retina

Progress in Retinal and Eye Research, Journal Year: 2020, Volume and Issue: 78, P. 100844 - 100844

Published: Feb. 5, 2020

This review summarizes our current knowledge of primate including human retina focusing on bipolar, amacrine and ganglion cells their connectivity. We have two main motivations in writing. Firstly, recent progress non-invasive imaging methods to study retinal diseases mean that better understanding the is becoming an important goal both for basic clinical sciences. Secondly, genetically modified mice are increasingly used as animal models diseases. Thus, it understand which extent retinas primates rodents comparable. first compare cell populations rodent retinas, with emphasis how fovea (despite its small size) dominates neural landscape retina. next summarise what known, not about postreceptoral neurone The inventories bipolar now nearing completion, comprising ~12 types at least 17 cell. Primate show clear differences dendritic field size across retina, morphology differs clearly from mouse cells. Compared cells, even higher morphological diversity: they could comprise over 40 types. Many appear conserved between mice, but functions only a few understood any or non-primate Amacrine final frontier research monkeys alike.

Language: Английский

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A cell atlas of the chick retina based on single-cell transcriptomics

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Jan. 4, 2021

Retinal structure and function have been studied in many vertebrate orders, but molecular characterization has largely confined to mammals. We used single-cell RNA sequencing (scRNA-seq) generate a cell atlas of the chick retina. identified 136 types plus 14 positional or developmental intermediates distributed among six classes conserved across vertebrates – photoreceptor, horizontal, bipolar, amacrine, retinal ganglion, glial cells. To assess morphology molecularly defined types, we adapted method for CRISPR-based integration reporters into selectively expressed genes. For Müller glia, found that transcriptionally distinct cells were regionally localized along anterior-posterior, dorsal-ventral, central-peripheral axes. also immature horizontal cell, oligodendrocyte persist late embryonic stages. Finally, analyzed relationships chick, mouse, primate types. Our results provide foundation anatomical, physiological, evolutionary, studies avian visual system.

Language: Английский

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Morphology and connectivity of retinal horizontal cells in two avian species

Frontiers in Cellular Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: March 4, 2025

In the outer vertebrate retina, visual signal is separated into intensity and wavelength information. birds, seven types of photoreceptors (one rod, four single cones, two members double cone) mediate signals to >20 second-order neurons, bipolar cells horizontal cells. Horizontal contribute color contrast processing by providing feedback feedforward fish, reptiles, amphibians they either encode or show color-opponent responses. Yet, for bird number cell not fully resolved even more importantly, synapses between have never been quantified any species. With a combination light microscopy serial EM reconstructions, we found different in distantly related species, domestic chicken European robin. agreement with some earlier studies, confirmed highly abundant (H1, H2) rare (H3, H4), which H1 an axon-bearing cell, whereas H2-H4 are axonless. made chemical one type interplexiform amacrine at their soma. Dendritic contacts H1-H4 were type-specific similar turtle confirms high degree evolutionary conservation retina. Our data further suggests that potentially H2 may intensity, H3 H4 represent opponent birds’ superb and/or acuity vision.

Language: Английский

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Neural circuits in the mouse retina support color vision in the upper visual field

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: July 13, 2020

Color vision is essential for an animal's survival. It starts in the retina, where signals from different photoreceptor types are locally compared by neural circuits. Mice, like most mammals, dichromatic with two cone types. They can discriminate colors only their upper visual field. In corresponding ventral however, cones display same spectral preference, thereby presumably impairing comparisons. this study, we systematically investigated retinal circuits underlying mouse color recording light responses cones, bipolar and ganglion cells. Surprisingly, color-opponent cells located rod photoreceptors likely being involved. Here, complexity of chromatic processing increases towards output, non-linear center-surround interactions create specific output channels to brain. This suggests that retina tuned extract field, aiding robust detection predators ensuring

Language: Английский

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Label-Free cancer cell biosensor based on photonic crystal ring resonator

Results in Physics, Journal Year: 2023, Volume and Issue: 46, P. 106317 - 106317

Published: March 1, 2023

A photonic crystal (PhC) biosensor is a compact device, which detects the changes of characteristic light due to binding sample by analyte. In this paper; we propose using ring resonator in two-dimensional (2D) biosensor. periodic array silicon rods with refractive index n = 3.46 air bacground formed cubic lattice structure. The sensing mechanism occures when resonant wavelength shifts change analytes. transmission spectrum about 4.47 nm higher wavelengths increasing This can recognize normal, and cancerous cells. average values sensitivity, quality factor, FOM, for designed structure are 308.5 (nm/RIU), 3803.55, 848.06 RIU-1 98.78 %, respectively. reported has high promising applications highly sensitive label-free optical biosensors.

Language: Английский

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Recommendations for measuring and standardizing light for laboratory mammals to improve welfare and reproducibility in animal research

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(3), P. e3002535 - e3002535

Published: March 12, 2024

Light enables vision and exerts widespread effects on physiology behavior, including regulating circadian rhythms, sleep, hormone synthesis, affective state, cognitive processes. Appropriate lighting in animal facilities may support welfare ensure that animals enter experiments an appropriate physiological behavioral state. Furthermore, proper consideration of light during experimentation is important both when it explicitly employed as independent variable a general feature the environment. This Consensus View discusses metrics to use for quantification nonhuman mammals their application improve quality research. It provides methods measuring these metrics, practical guidance implementation husbandry experimentation, quantitative exposure laboratory mammals. The provided has potential data contribute reduction refinement, helping more ethical use.

Language: Английский

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KIF11 UFMylation Maintains Photoreceptor Cilium Integrity and Retinal Homeostasis

Advanced Science, Journal Year: 2024, Volume and Issue: 11(25)

Published: April 26, 2024

Abstract The photoreceptor cilium is vital for maintaining the structure and function of retina. However, molecular mechanisms underlying integrity retinal homeostasis are largely unknown. Herein, it shown that kinesin family member 11 (KIF11) localizes at transition zone (connecting cilium) plays a crucial role in orchestrating integrity. KIF11 depletion causes malformations both ciliary axoneme membranous discs, resulting degeneration accumulation drusen‐like deposits throughout Mechanistic studies show stability regulated by an interplay between its UFMylation ubiquitination; lysine 953 inhibits ubiquitination synoviolin 1 thereby prevents proteasomal degradation. 953‐to‐arginine mutant more stable than wild‐type also effective reversing defects induced depletion. These findings identify critical maintenance homeostasis.

Language: Английский

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Ancient origin of the rod bipolar cell pathway in the vertebrate retina

Nature Ecology & Evolution, Journal Year: 2024, Volume and Issue: 8(6), P. 1165 - 1179

Published: April 16, 2024

Language: Английский

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Current Insights into 3D Bioprinting: An Advanced Approach for Eye Tissue Regeneration

Pharmaceutics, Journal Year: 2021, Volume and Issue: 13(3), P. 308 - 308

Published: Feb. 26, 2021

Three-dimensional (3D) printing is a game changer technology that holds great promise for wide variety of biomedical applications, including ophthalmology. Through this emerging technique, specific eye tissues can be custom-fabricated in flexible and automated way, incorporating different cell types biomaterials precise anatomical 3D geometries. However, despite the progress possibilities generated recent years, there are still challenges to overcome jeopardize its clinical application regular practice. The main goal review provide an in-depth understanding current status implementation bioprinting ophthalmology field order manufacture relevant such as cornea, retina conjunctiva. Special attention paid description most commonly employed methods, tissue engineering studies performed by at preclinical level. In addition, other issues related use ocular drug delivery, well both ethical regulatory aspects, analyzed. review, we aim raise awareness among research community report advances future directions apply advanced therapy regeneration field.

Language: Английский

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Topographic Distribution and Progression of Soft Drusen Volume in Age-Related Macular Degeneration Implicate Neurobiology of Fovea

Investigative Ophthalmology & Visual Science, Journal Year: 2021, Volume and Issue: 62(2), P. 26 - 26

Published: Feb. 19, 2021

Purpose: To refine estimates of macular soft drusen abundance in eyes with age-related degeneration (AMD) and evaluate hypotheses about biogenesis, we investigated topographic distribution growth rates by optical coherence tomography (OCT). We compared results to retinal features similar topographies (cone density pigment) healthy eyes. Methods: In a prospective study, AMD were identified human observers OCT volumes analyzed computer-assistance. Published histologic data for cone densities (n = 12 eyes) vivo pigment (MPOD) measurements older adults unremarkable maculae 31; 62 paired eyes, averaged) revisited. All values normalized Early Treatment Diabetic Retinopathy Study (ETDRS) subfield areas. Results: Sixty-two 44 patients imaged periods up 78 months. Soft volume per unit at baseline is 24.6-fold 2.3-fold higher the central ETDRS than outer inner rings, respectively, grows most prominently there. Corresponding ratios (central versus outer) donor 13.3-fold 5.1-fold MPOD, 24.6 23.9-fold, 3.6 3.6-fold. Conclusions: Normalized as assessed ≥ 20-fold subfields paralleling MPOD Data on support this metric risk assessment clinical trial outcome measure. Alignment different modalities grid standardizing topography mechanistic studies biogenesis.

Language: Английский

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