Sp1-Induced lncRNA Rmrp Promotes Mesangial Cell Proliferation and Fibrosis in Diabetic Nephropathy by Modulating the miR-1a-3p/JunD Pathway DOI Creative Commons
Hansen Yang, Jia Wang, Zheng Zhang

et al.

Frontiers in Endocrinology, Journal Year: 2021, Volume and Issue: 12

Published: Aug. 27, 2021

Diabetic nephropathy (DN) is a serious complication of diabetes mellitus. Long non-coding RNAs (lncRNAs) are regulators in DN progression. However, the regulatory mechanisms multiple lncRNAs remain to be determined. Our aim was investigate function and molecular mechanism lncRNA RNA component mitochondrial RNAase P (Rmrp) DN. Here, we observed that expression Rmrp up-regulated kidney db/db mice high glucose induced glomerular mesangial cells (MC). More importantly, abnormal transcription by nuclear factor Sp1, which promotes proliferation production fibrotic markers MC. Subsequently, screened miRNAs related found miR-1a-3p co-localized at subcellular level MC, could directly binds miR-1a-3p. Further research demonstrated elevated significantly attenuated fibrosis-promoting effects up-regulation Rmrp. At same time, also investigated can bind Jun D proto-oncogene (JunD), thereby regulating protein JunD. Rmrp-induced fibrogenesis were reversed co-transfection with JunD siRNA. In summary, Sp1 drive via sponging

Language: Английский

Research progress of non-coding RNA regulating the role of PANoptosis in diabetes mellitus and its complications DOI
Guangyu Han, Kaibo Hu, Tao Luo

et al.

APOPTOSIS, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 4, 2025

Language: Английский

Citations

3
Mechanisms, Biomarkers, and Treatment Approaches for Diabetic Kidney Disease: Current Insights and Future Perspectives DOI Open Access
Jean Paule Joumaa,

Angela Raffoul,

Claudia Sarkis

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(3), P. 727 - 727

Published: Jan. 23, 2025

Diabetic Kidney Disease (DKD) is the leading cause of end-stage renal disease (ESRD) worldwide. Among individuals with type 1 diabetes mellitus (T1DM), 30–40% are at risk developing DKD. This review focuses on mechanistic processes, available and emerging biomarkers for diagnosing, monitoring, preventing DKD, as well treatment options targeted DKD patients. A literature search was conducted PubMed Scopus using specific keywords. Inclusion exclusion criteria were applied to select articles used this review. The highlights various mechanisms involved in progression more severe stages. Additionally, several have been identified, which aid diagnosing monitoring disease. Furthermore, numerous approaches being explored address underlying causes Advanced research exploring new medications remission; sodium-glucose cotransport (SGLT2) inhibitors finerenone, particular, gaining attention their novel renoprotective effects. a major complication diabetes, marked by complex multifactorial mechanisms. Thus, understanding these processes essential therapies potentially reverse progression. Biomarkers show promise early diagnosis progression, while current strategies underscore importance multifaceted approach.

Language: Английский

Citations

2
Emerging Theranostic Nanomaterials in Diabetes and Its Complications DOI Creative Commons

Yuntao Liu,

Siqi Zeng, Wei Ji

et al.

Advanced Science, Journal Year: 2021, Volume and Issue: 9(3)

Published: Nov. 25, 2021

Diabetes mellitus (DM) refers to a group of metabolic disorders that are characterized by hyperglycemia. Oral subcutaneously administered antidiabetic drugs such as insulin, glipalamide, and metformin can temporarily balance blood sugar levels, however, long-term administration these therapies is associated with undesirable side effects on the kidney liver. In addition, due overproduction reactive oxygen species hyperglycemia-induced macrovascular system damage, diabetics have an increased risk complications. Fortunately, recent advances in nanomaterials provided new opportunities for diabetes therapy diagnosis. This review provides panoramic overview current detection diabetic biomarkers treatment. Apart from sensing mechanisms activities, applications bioengineered nanoparticles preventing several complications elucidated. overall perspective this field, including challenges future trends, which may be helpful informing development novel functions properties diagnosis therapy.

Language: Английский

Citations

103
A Novel Identified Circular RNA, mmu_mmu_circRNA_0000309, Involves in Germacrone-Mediated Improvement of Diabetic Nephropathy Through Regulating Ferroptosis by Targeting miR-188-3p/GPX4 Signaling Axis DOI
Juan Jin, Yunguang Wang, Danna Zheng

et al.

Antioxidants and Redox Signaling, Journal Year: 2021, Volume and Issue: 36(10-12), P. 740 - 759

Published: Dec. 16, 2021

Aims: Diabetic nephropathy (DN) is characterized by microalbuminuria, mainly associated with pathological and morphological alterations of podocyte. New drug targeting podocyte injury a promising approach for treating DN. The present study aimed at developing new Results: In this study, germacrone ameliorated kidney damage inhibited apoptosis in DN mouse model. Based on RNA-seq, mmu_mmu_circRNA_0000309, located host gene vascular endothelial zinc finger 1 (Vezf1), showed sharp decline mice remarkable recovery germacrone-challenged mice. mmu_circRNA_0000309 silence or miR-188-3p mimics abrogated the antiapoptosis anti-injury effects through aggravating mitochondria damage, elevating reactive oxygen species ferroptosis-related protein levels. Mechanistically, competitively sponged miR-188-3p, subsequently promoted glutathione peroxidase 4 (GPX4) expression, thereby inactivating ferroptosis-dependent mitochondrial apoptosis. addition, GPX4 overexpression neutralized silence-mediated ferroptosis germacrone-exposed MPC5 cells. Innovation: We describe novel effect mechanism DN, which linked to first time. Conclusion: mediates resistance sponges upregulates function Possibly germacrone-based treatment can be further motivated regulating mmu_circRNA_0000309/miR-188-3p/GPX4 signaling axis. Antioxid. Redox Signal. 36, 740-759.

Language: Английский

Citations

75
Stem cell-derived and circulating exosomal microRNAs as new potential tools for diabetic nephropathy management DOI Creative Commons
Lei Peng, Yu Chen, Shaoqing Shi

et al.

Stem Cell Research & Therapy, Journal Year: 2022, Volume and Issue: 13(1)

Published: Jan. 24, 2022

Abstract Background Despite major advances in the treatment of diabetic nephropathy (DN) recent years, it remains most common cause end-stage renal disease. An early diagnosis and therapy may slow down DN progression. Numerous potential biomarkers are currently being researched. Circulating levels kidney-released exosomes biological molecules, which reflect pathology including glomerular tubular dysfunction as well mesangial expansion fibrosis, have shown for predicting occurrence progression DN. Moreover, many experimental therapies investigated, stem cell medications targeting inflammatory, oxidant, or pro-fibrotic pathways activated during The therapeutic cells is partly depending on their secretory capacity, particularly exosomal microRNAs (Exo-miRs). In a growing line research has participation Exo-miRs pathophysiological processes DN, provide effective biomarker tools treatment. Methods A systematic literature search was performed MEDLINE, Scopus, Google Scholar to collect published findings regarding cell-derived circulating DN-associated biomarkers. Findings Glomerular podocytes important culprits pathogenesis and, thus, can be considered valuable targets. Preclinical investigations that exert beneficial effects by transferring renoprotective miRs injured podocytes. Of note, Exo-miR-125a secreted adipose-derived mesenchymal improve cells, while (Exo-miR-486 Exo-miR-215-5p), human urine‐derived (Exo-miR-16-5p), bone marrow-derived (Exo-miR-let-7a) On other hand, clinical indicated isolated from urine serum hold great promising

Language: Английский

Citations

42
Novel Insights into Diabetic Kidney Disease DOI Open Access
Ewelina Młynarska, Dominika Buławska, Witold Czarnik

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10222 - 10222

Published: Sept. 23, 2024

Diabetic kidney disease (DKD) is a major complication of diabetes mellitus (DM), affecting over one-third type 1 and nearly half 2 patients. As the leading cause end-stage renal (ESRD) globally, DKD develops through complex interplay chronic hyperglycemia, oxidative stress, inflammation. Early detection crucial, with diagnosis based on persistent albuminuria reduced estimated glomerular filtration rate (eGFR). Treatment strategies emphasize comprehensive management, including glycemic control, blood pressure regulation, use nephroprotective agents such as angiotensin-converting enzyme (ACE) inhibitors, angiotensin II receptor blockers (ARBs), sodium-glucose cotransporter-2 (SGLT2) glucagon-like peptide-1 (GLP-1) agonists. Ongoing research explores novel therapies targeting molecular pathways non-coding RNAs. Preventive measures focus rigorous control hyperglycemia hypertension, aiming to mitigate progression. Despite therapeutic advances, remains ESRD, highlighting need for continued identify new biomarkers innovative treatments.

Language: Английский

Citations

15
Epigenetic modifications in diabetes DOI
Renu A. Kowluru, Ghulam Mohammad

Metabolism, Journal Year: 2021, Volume and Issue: 126, P. 154920 - 154920

Published: Oct. 27, 2021

Language: Английский

Citations

53
Exploring the Therapeutic Significance of microRNAs and lncRNAs in Kidney Diseases DOI Open Access
Luis Alberto Bravo-Vázquez, Sujay Paul,

Miriam Guadalupe Colín-Jurado

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(1), P. 123 - 123

Published: Jan. 19, 2024

MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are two crucial classes of transcripts that belong to the major group (ncRNAs). These RNA molecules have significant influence over diverse molecular processes due their role as regulators gene expression. However, dysregulated expression these ncRNAs constitutes a fundamental factor in etiology progression wide variety multifaceted human diseases, including kidney diseases. In this context, past years, compelling evidence has shown miRNAs lncRNAs could be prospective targets for development next-generation drugs against diseases they participate number disease-associated processes, such podocyte nephron death, renal fibrosis, inflammation, transition from acute injury chronic disease, vascular changes, sepsis, pyroptosis, apoptosis. Hence, current review, we critically analyze recent findings concerning therapeutic inferences pathophysiological context Additionally, with aim driving advances formulation ncRNA-based tailored management discuss some key challenges future prospects should addressed forthcoming investigations.

Language: Английский

Citations

8
Serum exosomes from diabetic kidney disease patients promote pyroptosis and oxidative stress through the miR-4449/HIC1 pathway DOI Creative Commons

Chan Gao,

Benyong Wang,

Qi Chen

et al.

Nutrition and Diabetes, Journal Year: 2021, Volume and Issue: 11(1)

Published: Nov. 3, 2021

Diabetic kidney disease (DKD) is a major contributor to end-stage renal disease. Several microRNAs (miRNAs) have been found be enriched in exosomes of DKD patients, but it remains unclear if any these miRNAs play an important role the pathogenesis DKD.Exosomes from diabetic patients were isolated, and expression miR-4449 was measured by qRT-PCR. Reactive oxygen species (ROS) determined DCDFA assay kit, pyroptosis quantifying level activated caspase 1. mRNA protein levels quantified qRT-PCR WB.In this study, we demonstrated that serum regulate pro-inflammatory cytokines, ROS levels, through miR-4449.Our study uncovered novel mechanism for progression mediated exosomes, which highlights DKD.

Language: Английский

Citations

34
Urinary exosomal microRNA-145-5p and microRNA-27a-3p act as noninvasive diagnostic biomarkers for diabetic kidney disease DOI Open Access
Lulu Han, Shenghai Wang,

Mingyan Yao

et al.

World Journal of Diabetes, Journal Year: 2024, Volume and Issue: 15(1), P. 92 - 104

Published: Jan. 12, 2024

Diabetic kidney disease (DKD), characterized by increased urinary microalbumin levels and decreased renal function, is the primary cause of end-stage disease. Its pathological mechanisms are complicated multifactorial; Therefore, sensitive specific biomarkers needed. Urinary exosome originate from diverse cells in nephron segments partially mirror changes kidney. The microRNAs (miRNAs) remarkably stable highly tissue-specific for

Language: Английский

Citations

5