Identification of the optimal reference genes for atrial fibrillation model established by iPSC-derived atrial myocytes DOI Creative Commons
Lei M. Li,

Zijuan Zhao,

Zihao Liu

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 25, 2024

Atrial fibrillation (AF) stands as a prevalent and detrimental arrhythmic disorder, characterized by intricate pathophysiological mechanisms. The availability of reliable reproducible AF models is pivotal in unraveling the underlying mechanisms this complex condition. Unfortunately, researchers are still confronted with absence consistent vitro models, hindering progress crucial area research. Human induced pluripotent stem cells derived atrial myocytes (hiPSC-AMs) were generated based on GiWi methods verified whole-cell patch clamp, immunofluorescent staining, flow cytometry. Then hiPSC-AMs employed to establish model HS. Whole-cell clamp technique calcium imaging used identify model. stability 29 reference genes was evaluated using delta-Ct, GeNorm, NormFinder, BestKeeper algorithms; HiPSC-AMs displayed myocyte action potentials expressed atrial-specific protein MLC-2 A NR2F2, about 70% cardiomyocytes positive. After HS, showed significant increase beating frequency, shortened potential duration, increased transient frequency. Of candidate genes, top five most stably ranked ABL1, RPL37A, POP4, RPL30, EIF2B1. normalization KCNJ2 significantly upregulated model; Conclusions: In established ABL1 provides greater efficiency than commonly GAPDH.

Language: Английский

Spexin inhibits excessive autophagy‐induced ferroptosis to alleviate doxorubicin‐induced cardiotoxicity by upregulating Beclin 1 DOI

Wen Ou,

Haiqiong Liu,

Changhai Chen

et al.

British Journal of Pharmacology, Journal Year: 2024, Volume and Issue: 181(21), P. 4195 - 4213

Published: July 3, 2024

Doxorubicin is widely used in the treatment of malignant tumours, but doxorubicin-induced cardiotoxicity severely limits its clinical application. Spexin a neuropeptide that acts as novel biomarker cardiovascular disease. However, effects spexin on unclear.

Language: Английский

Citations

7
Spexin (Neuropeptide Q): Distribution, Functional Roles, and Emerging Therapeutic Perspectives DOI

Ritesh S. Tarwani,

Kamini R. Shirasath,

Sanjay N. Awathale

et al.

International Journal of Peptide Research and Therapeutics, Journal Year: 2025, Volume and Issue: 31(2)

Published: Jan. 8, 2025

Language: Английский

Citations

0
Iron homeostasis and ferroptosis in muscle diseases and disorders: mechanisms and therapeutic prospects DOI Creative Commons
Qin Ru, Yusheng Li, Feng Zhang

et al.

Bone Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: Feb. 25, 2025

Abstract The muscular system plays a critical role in the human body by governing skeletal movement, cardiovascular function, and activities of digestive organs. Additionally, muscle tissues serve an endocrine function secreting myogenic cytokines, thereby regulating metabolism throughout entire body. Maintaining requires iron homeostasis. Recent studies suggest that disruptions ferroptosis, form iron-dependent cell death, are essential contributors to progression wide range diseases disorders, including sarcopenia, cardiomyopathy, amyotrophic lateral sclerosis. Thus, comprehensive overview mechanisms ferroptosis these conditions is crucial for identifying potential therapeutic targets developing new strategies disease treatment and/or prevention. This review aims summarize recent advances understanding molecular underlying context injury, as well associated disorders. Moreover, we discuss within pathway possible managing Finally, shed light on current limitations future prospects interventions targeting ferroptosis.

Language: Английский

Citations

0
Inflammation pathways as therapeutic targets in angiotensin II induced atrial fibrillation DOI Creative Commons

Ailin Hou,

Dazhuo Shi,

Hongbo Huang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 3, 2025

Atrial fibrillation (AF), a common cardiac arrhythmia, is associated with severe complications such as stroke and heart failure. Although the precise mechanisms underlying AF remain elusive, inflammation acknowledged pivotal factor in its progression. Angiotensin II (AngII) implicated promoting atrial remodeling inflammation. However, exact pathways through which AngII exacerbates are still not fully defined. This study explores key molecular involved, including dysregulation of calcium ions, altered connexin expression, activation signaling TGF-β, PI3K/AKT, MAPK, NF-κB/NLRP3, Rac1/JAK/STAT3. These instrumental contributing to fibrosis, electrical remodeling, increased susceptibility AF. Ang II-induced disrupts ion channel function, resulting structural atria significantly elevating risk Anti-inflammatory treatments RAAS inhibitors, colchicine, statins have demonstrated potential reducing incidence AF, although clinical outcomes inconsistent. manuscript underscores link between AngII-induced development proposing importance targeting management

Language: Английский

Citations

0
Novel hypothalamic pathways for metabolic effects of spexin DOI Creative Commons
Penghua Fang,

Yuqing She,

Mei Yu

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 208, P. 107399 - 107399

Published: Sept. 7, 2024

One of the main underlying etiologies type 2 diabetes (T2DM) is insulin resistance, which most frequently caused by obesity. Notably, deregulation adipokine secretion from visceral adiposity has been identified as a crucial characteristic and Spexin an that released many different tissues, including white adipocytes glandular stomach, negatively connected with state energy storage. This peptide acts through GALR2/3 receptors to control wide range metabolic processes, inflammation, browning, lipolysis, expenditure, eating behavior. Specifically, spexin can enter hypothalamus regulate hypothalamic melanocortin system, in turn balances expenditure food intake. review examines recent advances mechanisms obesity T2DM. In particular, we address topics basic research clinical findings, such analysis possible function response, involves reducing intake increasing while also enhancing sensitivity glucose tolerance. Gaining more insight into underlie system's over metabolism homeostasis may facilitate development innovative treatment approaches focus on combating diabetes.

Language: Английский

Citations

1
Spexin Hormone Signaling and Atrial Fibrillation: The Knowns and Unknowns DOI
Jordi Heijman, Dobromir Dobrev

Circulation, Journal Year: 2024, Volume and Issue: 150(2), P. 128 - 131

Published: July 8, 2024

Language: Английский

Citations

0
Identification of the optimal reference genes for atrial fibrillation model established by iPSC-derived atrial myocytes DOI Creative Commons
Lei M. Li,

Zijuan Zhao,

Zihao Liu

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 25, 2024

Atrial fibrillation (AF) stands as a prevalent and detrimental arrhythmic disorder, characterized by intricate pathophysiological mechanisms. The availability of reliable reproducible AF models is pivotal in unraveling the underlying mechanisms this complex condition. Unfortunately, researchers are still confronted with absence consistent vitro models, hindering progress crucial area research. Human induced pluripotent stem cells derived atrial myocytes (hiPSC-AMs) were generated based on GiWi methods verified whole-cell patch clamp, immunofluorescent staining, flow cytometry. Then hiPSC-AMs employed to establish model HS. Whole-cell clamp technique calcium imaging used identify model. stability 29 reference genes was evaluated using delta-Ct, GeNorm, NormFinder, BestKeeper algorithms; HiPSC-AMs displayed myocyte action potentials expressed atrial-specific protein MLC-2 A NR2F2, about 70% cardiomyocytes positive. After HS, showed significant increase beating frequency, shortened potential duration, increased transient frequency. Of candidate genes, top five most stably ranked ABL1, RPL37A, POP4, RPL30, EIF2B1. normalization KCNJ2 significantly upregulated model; Conclusions: In established ABL1 provides greater efficiency than commonly GAPDH.

Language: Английский

Citations

0