Frontiers in Physiology,
Journal Year:
2024,
Volume and Issue:
15
Published: Nov. 20, 2024
Repetitive
variations,
such
as
oscillation,
are
ubiquitous
in
biology.
In
this
mini
review,
we
present
a
general
summary
of
the
∼24
h
circadian
clock
and
provide
fundamental
overview
another
biological
timekeeper
that
maintains
∼12
oscillations.
This
oscillator
is
proposed
to
function
independently
regulate
ultradian
rhythms
relevant
both
protein
homeostasis
liver
health.
Recent
studies
exploring
these
humans
discussed,
followed
by
our
proposal
mammary
gland
physiology
represents
promising
area
for
further
research.
We
conclude
highlighting
potential
translational
applications
chronobiology.
Frontiers in Nutrition,
Journal Year:
2023,
Volume and Issue:
10
Published: March 16, 2023
Nutritional
and
food
components
reshape
the
peripheral
clock
metabolism.
However,
whether
challenges
affect
circadian
metabolism
of
meibomian
glands
(MGs)
has
not
been
fully
explored.
This
study
was
designed
to
analyze
alterations
in
rhythmic
transcriptome
MGs
murine
fed
a
balanced
diet
or
high-fat
(HFD).
Male
C57BL/6J
mice
were
maintained
on
12/12
h
light/dark
cycle
ad
libitum
normal
chow
(NC)
HFD
for
4
weeks.
collected
from
sacrificed
animals
at
3-h
intervals
throughout
24-h
cycle.
The
analyzed
via
bioinformatics
approaches
using
high-throughput
RNA
sequencing
(RNA-seq).
In
addition,
oscillations
lipid
analyzed.
Meibomian
displayed
robust
rhythmicity.
feeding
significantly
altered
profile
MGs-including
composition
phase-and
spatiotemporally
affected
enriched
signaling
pathways.
MGs.
Our
data
show
that
affects
MGs'
rhythmicity,
which
reveals
high
sensitivity
clocks
food.
Investigative Ophthalmology & Visual Science,
Journal Year:
2023,
Volume and Issue:
64(13), P. 3 - 3
Published: Oct. 3, 2023
Purpose:
Diabetic
retinopathy
(DR)
is
a
common
complication
of
diabetes
and
has
high
prevalence.
Dysregulation
circadian
rhythmicity
associated
with
the
development
DR.
This
research
aimed
to
investigate
rhythmical
transcriptome
alterations
in
retina
diabetic
mice.
Methods:
C57BL/6J
mice
were
used
establish
model
by
intraperitoneal
injection
streptozotocin
(STZ).
After
12
weeks,
retinas
collected
continuously
at
4-hour
intervals
over
1
day.
Total
RNA
was
extracted
from
normal
STZ-treated
sequencing
performed.
Meta2d
algorithm,
Kyoto
Encyclopedia
Genes,
Phase
Set
Enrichment
Analysis,
time-series
cluster
analysis
identify,
analyze
annotate
composition,
phase,
molecular
functions
rhythmic
transcripts
retinas.
Results:
The
exhibited
powerful
rhythmicity.
STZ-induced
markedly
modified
characteristics
retina,
including
amplitude.
Moreover,
led
re-organized
temporal
clustering
enrichment
pathways
space
time
affected
core
clock
machinery.
Conclusions:
Diabetes
impairs
rhythm
transcriptomic
profile
study
offers
new
perspectives
on
negative
effects
which
may
provide
important
information
for
treatments
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: April 2, 2024
Purpose
To
determine
the
effects
of
EV-A71
(Enterovirus
A71)
infection
on
ocular
surface
and
its
mechanism.
Methods
AG6
mice
aged
two
to
three
weeks
were
randomly
divided
into
control
infected
groups.
Slit-lamp
observation,
fluorescein
staining,
phenol
red
thread
test
used
assess
symptoms
at
4
dpi
(days
post
infection).
The
pathological
changes
cornea
lacrimal
gland
observed
by
H&E
PAS
TUNEL
assay,
IHC
staining
qRT-PCR.
Corneas
glands
from
obtained
processed
for
RNA
sequencing
analysis.
Newly
diagnosed
HFMD
patients
caused
recruited
ensured
they
met
inclusion
criteria.
Ocular
parameters
(TMH
NIKBUT)
measured
using
OCULUS
Keratograph
5M.
Tear
samples
taken
examine
Cxcl1
IL-6
levels
through
ELISA
method.
Results
Mice
studies
revealed
that
tear
film
instability,
decreased
secretions,
in
size,
distinct
goblet
cell
loss.
It
also
resulted
increased
large
vacuoles
within
acinar
cells
structural
damage
gland.
Apart
minor
epidermis,
there
was
no
obvious
inflammatory
or
apoptosis
cornea.
However,
significant
injury
RNA-seq
analysis
showed
IL-17
NF-κB
signaling
pathways
activated
with
EV-A71.
In
patients,
THM
a
low
range
NITBUT
significantly
shorter
than
group
Oculus
assay
higher
level
them.
Conclusion
affected
structure
function
induced
dry
eye-like
symptoms.
Frontiers in Physiology,
Journal Year:
2024,
Volume and Issue:
15
Published: Nov. 20, 2024
Repetitive
variations,
such
as
oscillation,
are
ubiquitous
in
biology.
In
this
mini
review,
we
present
a
general
summary
of
the
∼24
h
circadian
clock
and
provide
fundamental
overview
another
biological
timekeeper
that
maintains
∼12
oscillations.
This
oscillator
is
proposed
to
function
independently
regulate
ultradian
rhythms
relevant
both
protein
homeostasis
liver
health.
Recent
studies
exploring
these
humans
discussed,
followed
by
our
proposal
mammary
gland
physiology
represents
promising
area
for
further
research.
We
conclude
highlighting
potential
translational
applications
chronobiology.
