Shenling Guchang prescription ameliorates intestinal barrier inflammation in gestational diabetes rats <i>via</i> TLR4/NF-κB pathway DOI Open Access
Manling Li, Lisha Li,

Xingman Liu

et al.

Drug Discoveries & Therapeutics, Journal Year: 2024, Volume and Issue: 18(6), P. 343 - 352

Published: Dec. 11, 2024

Gestational diabetes mellitus (GDM) is linked to a greater risk of various maternal and fetal complications, including the possibility long-term metabolic issues in offspring. Our initial research suggests that Traditional Chinese Medicine formula, Shenling Guchang prescription (SLGP), may have an impact on gut microbiota. However, specific mechanisms through which it affects intestinal barrier inflammation GDM are still not fully understood. This study explored SLGP's GDM. Firstly, network pharmacology predicted key bioactive constituents targeting toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB), guiding experimental design. Subsequently, pregnant female rats were induced with intraperitoneal streptozotocin injection then divided into control, model, metformin, SLGP treatment groups. Blood samples collected for ELISA analysis measure levels inflammatory markers, tissues examined histologically using hematoxylin-eosin (HE) staining, western blot was conducted evaluate TLR4 NF-κB expression. Relative control rats, model group animals exhibited significant increases markers (IL-1β, IL-6, TNF-α, TGF-β, CRP), as well enhanced p-NF-κB p65 expression, along histopathological changes. Treatment notably reduced protein expression colonic tissue leading decrease damage. Overall, found modulate TLR4/NF-κB pathway, resulting enhancements insulin resistance reduction responses thereby providing protection intestines. demonstrates potential therapeutic effectiveness addressing

Language: Английский

Maternal gut microbiota in the health of mothers and offspring: from the perspective of immunology DOI Creative Commons

Xiaowen Lu,

Zhan Shi, Lingling Jiang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 13, 2024

Due to the physiological alteration during pregnancy, maternal gut microbiota changes following metabolic processes. Recent studies have revealed that is closely associated with immune microenvironment in utero pregnancy and plays a vital role specific complications, including preeclampsia, gestational diabetes, preterm birth recurrent miscarriages. Some other evidence has also shown aberrant increases risk of various diseases offspring, such as allergic neurodevelopmental disorders, through alignment between mother fetus possible intrauterine microbiota. Probiotics high-fiber diet are effective inventions prevent mothers fetuses from diseases. In this review, we summarize development complications health condition future generations perspective immunology, which may provide new therapeutic strategies for management offspring.

Language: Английский

Citations

16
Butyrate: a bridge between intestinal flora and rheumatoid arthritis DOI Creative Commons
Cao Yang,

Jingjing Chen,

Jing Xiao

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 16, 2024

In patients with rheumatoid arthritis (RA), intestinal flora imbalance and butyrate metabolism disorders precede clinical are associated the pathogenesis of RA. This can alter immunology permeability mucosa, leading to damage barrier. this context, bacteria their metabolites enter bloodstream reach distant target tissues host, resulting in local inflammation aggravating arthritis. Additionally, is also exacerbated by bone destruction immune tolerance due disturbed differentiation osteoclasts adaptive cells. Of note, a metabolite flora, which not only locally inhibits immunity targets zonulin tight junction proteins alleviate barrier-mediated but autoantibodies balances responses T B lymphocytes throughout body repress erosion inflammation. Therefore, key intermediate linking host. As result, restoring butyrate-producing capacity using exogenous potential therapeutic strategies for RA future.

Language: Английский

Citations

6
The perinatal microbiota-gut-brain axis: Implications for postpartum depression DOI Creative Commons
Marie Armbruster, Paul Forsythe

NeuroImmunoModulation, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 25

Published: Jan. 21, 2025

Background: Pregnancy and childbirth are accompanied by widespread maternal physiological adaptations hormonal shifts, that have been suggested to result in a period of vulnerability for the development mood disorders such as postpartum depression (PPD). There is also evidence peripartum changes composition gut microbiota, but potential contribution intestinal microbes adaptations, or subsequent vulnerabilities, during this unknown. Summary: Here we outline key pathways involved including GABAergic signaling, oxytocin immunomodulation associated with susceptibility present these modulated microbes. We discuss therapeutic microbiota-gut-brain axis identify future directions research help realize potential. Key Messages: Peripartum shifts microbial composition. Disruption GABAergic, immunomodulatory may contribute depression. These adaptative suggesting role microbiota determining PPD. More needed confirm relationship PPD gain mechanistic understanding required disorder.

Language: Английский

Citations

0
The influence of the gut microbiota on B cells in autoimmune diseases DOI Creative Commons

Lun He,

Xin Li, Shan Jiang

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: April 22, 2025

Abstract Mounting evidence shows that gut microbiota communities and the human immune system coexist influence each other, there are a number of reports correlation between specific changes in occurrence autoimmune diseases. B lymphocytes play central role regulation both Here, we summarize microbiota-B cell pathways on diseases how cells regulate microorganisms, which provides mechanistic insights with relevance for identification potential therapeutic targets related fields.

Language: Английский

Citations

0
Nutrition during pregnancy: Influence on the gut microbiome and fetal development DOI Creative Commons
Gabriela Barrientos, Francesca Ronchi, Melanie L. Conrad

et al.

American Journal of Reproductive Immunology, Journal Year: 2023, Volume and Issue: 91(1)

Published: Dec. 11, 2023

Pregnancy is a finely tuned process, with the health and well-being of developing fetus determined by metabolic status dietary intake mother. The maternal gut microbiome remodeled during pregnancy, this, coupled nutrient gestation shapes production metabolites that can cross placenta affect fetal development. As posited Developmental Origins Health Disease Hypothesis, such environmental influences have major effects on organ systems. When occurring at particularly sensitive gestational time points, these developmental programming events long lasting offspring adaptation to postnatal environment, implications later in life. This review will summarize current knowledge how pregnancy intrinsically extrinsically modify microbiota composition metabolite production. Further, we assess factors shape landscape ultimately contribute health. DOHaD, development, metabolites, microbiome, nutrition, short-chain fatty acids.

Language: Английский

Citations

9
Relationship between gut microbiota and the pathogenesis of gestational diabetes mellitus: a systematic review DOI Creative Commons

Sheng Ma,

Yuping Wang,

Xiaoxia Ji

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: May 28, 2024

Introduction Gestational diabetes mellitus (GDM) is a form of gestational characterized by insulin resistance and abnormal function pancreatic beta cells. In recent years, genomic association studies have revealed risk susceptibility genes associated with genetic to GDM. However, predisposition cannot explain the rising global incidence GDM, which may be related increased influence environmental factors, especially gut microbiome. Studies shown that microbiota closely occurrence development This paper reviews relationship between pathological mechanism in order better understand role provide theoretical basis for clinical application treatment diseases. Methods The current research results on interaction GDM were collected analyzed through literature review. Keywords such as "GDM", "gut microbiota" "insulin resistance" used search, methodology, findings potential impact pathophysiology systematically evaluated. Results It was found composition diversity significantly Specifically, abundance certain bacteria an while other changes microbiome improved sensitivity. addition, alterations affect blood glucose control variety mechanisms, including production short-chain fatty acids, activation inflammatory pathways, metabolism B vitamin group. Discussion this highlight importance pathogenesis regulation new directions improving sensitivity sugar use probiotics prebiotics. more needed confirm generality exact mechanisms these explore applications management diabetes. future should consider factors how together they

Language: Английский

Citations

2
Galactooligosaccharide Mediates NF-κB Pathway to Improve Intestinal Barrier Function and Intestinal Microbiota DOI Creative Commons

Menglu Xi,

Hao Guo,

Yao Qi

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(22), P. 7611 - 7611

Published: Nov. 15, 2023

The use of antibiotics to treat diarrhea and other diseases early in life can lead intestinal disorders infants, which cause a range immune-related diseases. Intestinal microbiota diversity is closely related dietary intake, with many oligosaccharides impacting microorganism structures communities. Thus, oligosaccharide type quantity are important for construction. Galactooligosaccharides (GOS) functional that be supplemented infant formula. Currently, information on GOS its impact lacking. Similarly, rarely reported within the context barrier function. In this study, 16S rRNA sequencing, gas chromatography, immunohistochemistry were used investigate effects pathways antibiotic-treated mouse models. results found promoted Bifidobacterium Akkermansia proliferation, increased short-chain fatty acid levels, tight junction protein expression (occludin ZO-1), secretory immunoglobulin A (SIgA) albumin significantly downregulated NF-κB expression, reduced lipopolysaccharide (LPS), interleukin-IL-1β (IL-1β), IL-6 levels. Also, high dose ampicillin-supplemented animals provided resistance damage.

Language: Английский

Citations

6
Modulatory effects of gut microbiota on innate and adaptive immune responses following spinal cord injury DOI
Zhu Li,

Fangfang Wang,

Jia-jia xing

et al.

Experimental Neurology, Journal Year: 2024, Volume and Issue: 379, P. 114866 - 114866

Published: June 13, 2024

Language: Английский

Citations

0
Shenling Guchang prescription ameliorates intestinal barrier inflammation in gestational diabetes rats <i>via</i> TLR4/NF-κB pathway DOI Open Access
Manling Li, Lisha Li,

Xingman Liu

et al.

Drug Discoveries & Therapeutics, Journal Year: 2024, Volume and Issue: 18(6), P. 343 - 352

Published: Dec. 11, 2024

Gestational diabetes mellitus (GDM) is linked to a greater risk of various maternal and fetal complications, including the possibility long-term metabolic issues in offspring. Our initial research suggests that Traditional Chinese Medicine formula, Shenling Guchang prescription (SLGP), may have an impact on gut microbiota. However, specific mechanisms through which it affects intestinal barrier inflammation GDM are still not fully understood. This study explored SLGP's GDM. Firstly, network pharmacology predicted key bioactive constituents targeting toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB), guiding experimental design. Subsequently, pregnant female rats were induced with intraperitoneal streptozotocin injection then divided into control, model, metformin, SLGP treatment groups. Blood samples collected for ELISA analysis measure levels inflammatory markers, tissues examined histologically using hematoxylin-eosin (HE) staining, western blot was conducted evaluate TLR4 NF-κB expression. Relative control rats, model group animals exhibited significant increases markers (IL-1β, IL-6, TNF-α, TGF-β, CRP), as well enhanced p-NF-κB p65 expression, along histopathological changes. Treatment notably reduced protein expression colonic tissue leading decrease damage. Overall, found modulate TLR4/NF-κB pathway, resulting enhancements insulin resistance reduction responses thereby providing protection intestines. demonstrates potential therapeutic effectiveness addressing

Language: Английский

Citations

0