IGF2 secreted from mesenchymal stem cells with high glutathione levels alleviates osteoarthritis via paracrine rejuvenation of senescent chondrocytes

Biomaterials Research, Journal Year: 2025, Volume and Issue: 29

Published: Jan. 1, 2025

Senescent chondrocytes, which are increased in osteoarthritic (OA) cartilage, promote cartilage defects and the senescent knee microenvironment by inducing senescence to surrounding normal chondrocytes secreting senescence-associated secretory proteins. Many studies have used mesenchymal stem cells (MSCs) treat OA, but MSC treatment remains challenging for clinical application owing quality control, engraftment, fibrocartilage regeneration. Here, rather than relying on direct regeneration of MSCs, we present a novel strategy suppress OA MSC-mediated chondrocyte targeting via paracrine activity thereby improving microenvironment. First, enable control umbilical cord priming MSCs supplementing human platelet lysate (hPL) greatly enhanced functions increasing cellular glutathione levels throughout serial passaging. Intra-articular injection primed successfully suppressed progression accumulation without Indirect coculture with using transwell ameliorated phenotypes suggesting rejuvenation. Based secretome analysis, identified insulin-like growth factor 2 (IGF2) as key component that induces rejuvenation MSCs. The effects IGF2 act through autophagy activation up-regulation autophagy-related gene expression autophagic flux. To cross-validate secreted vivo, knockdown substantially abolished its therapeutic efficacy rabbit model. Collectively, these findings demonstrate hPL supplementation enables levels. mechanism was IGF2, activating autophagy.

Language: Английский

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Anti-Aging Potential of Avocado Oil via Its Antioxidant Effects

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(2), P. 246 - 246

Published: Feb. 12, 2025

Aging is a process characterized by tissue degeneration, increased susceptibility to chronic degenerative diseases, infections, and the appearance of neoplasms, which leads disability reduction in length quality life. This phenomenon result convergence multiple processes, including mitochondrial dysfunction, fibrosis, inflammation, dysregulation cell death immunosenescence. These processes have as their point an increase production ROS. Avocado oil (Persea americana Mill.) contains diverse array bioactive compounds, oleic acid, phytosterols, chlorophylls, xanthones, xanthines, carotenoids. compounds capacity modulate excessive ROS, thereby reducing progression age-related diseases extending lifespan experimental models aging. In addition, several studies demonstrated efficacy avocado mitigating hypertension; insulin resistance; diabetes; non-alcoholic liver disease; such hearing loss, cognitive decline, neurodegeneration, impaired wound healing. light these findings, it hypothesized that promising agent capable promoting healthspan later stages life owing its direct antioxidant actions activation pathways enhance endogenous levels.

Language: Английский

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Carotenoids for Antiaging: Nutraceutical, Pharmaceutical, and Cosmeceutical Applications

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 403 - 403

Published: March 13, 2025

Background: Carotenoids are bioactive tetraterpenoid C40 pigments that actively synthesized by plants, bacteria, and fungi. Compounds such as α-carotene, β-carotene, lycopene, lutein, astaxanthin, β-cryptoxanthin, fucoxanthin, zeaxanthin have attracted increasing attention for their antiaging properties. They exhibit antioxidant, neuroprotective, anti-inflammatory properties, contributing to the prevention treatment of age-related diseases. Objectives: The aim this study was comprehensively analyze pharmacological potential biological mechanisms carotenoids associated with disorders evaluate application in nutraceuticals, pharmaceuticals, cosmeceuticals. Methods: A systematic review studies published over past two decades conducted using databases PubMed, Scopus, Web Science. selection criteria included clinical, silico, vivo, vitro investigating therapeutic effects carotenoids. Results: demonstrate a variety health benefits, including macular degeneration, cancer, cognitive decline, metabolic disorders, skin aging. Their role nutraceuticals is well supported ability modulate oxidative stress inflammatory pathways. In show promising results formulations targeting neurodegenerative diseases disorders. cosmeceuticals, they improve protecting it against UV radiation damage. However, bioavailability, optimal dosages, toxicity, interactions other compounds remain critical factors maximize efficacy still require careful evaluation scientists. Conclusions: interventions applications fields. Further research needed optimize formulas, confirm long-term safety effectiveness, especially aging population.

Language: Английский

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KAT7 contributes to ponatinib-induced hypertension by promoting endothelial senescence and inflammatory responses through activating NF-κB signaling pathway

Journal of Hypertension, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Background and purpose: Ponatinib, a tyrosine kinase inhibitor (TKI) leads to hypertension; however, the mechanisms remain elusive. We aimed investigate whether lysine acetyltransferase 7 (KAT7), key regulator of cellular senescence that is closely associated with cardiovascular diseases, involves in ponatinib-induced hypertension. Methods results: After administering ponatinib Sprague–Dawley (SD) rats for 8 days, we measured blood pressure, vasodilation, endothelial function using tail-cuff plethysmography, isometric myography, Total NO Assay kit, respectively. The results indicated increased impaired endothelium-dependent relaxation (EDR), caused injury cells SD rats. Furthermore, PCR Western blot experiments demonstrated an upregulation KAT7 expression rat mesenteric artery (MAECs) following treatment. To further study role hypertension, divided into four groups: control, ponatinib, WM-3835 (a inhibitor), plus WM-3835. Notably, administration significantly improved hypertension EDR dysfunction Mechanistically, over-expression (OE-KAT7) MAECs led inflammation, phenomena were also observed arteries ponatinib-treated exposed ponatinib. However, mitigated these detrimental effects both vivo vitro experiments. Additionally, OE-KAT7 treatment induced H3K14 acetylation (H3K14ac), elevating recruitment H3K14ac p21 promoter. Moreover, BAY 11-7085, nuclear factor (NF)-κB inhibitor, potently alleviated accumulation IL-6 IL-8, as well cell by overexpression. Conclusion: Our data indicate elevation inflammatory responses through NF-κB signaling pathway, subsequently vasotoxicity

Language: Английский

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Rapamycin ameliorates senescence of periodontal ligament stem cells and promotes their osteogenesis via the PI3K/AKT pathway

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 153, P. 114517 - 114517

Published: March 23, 2025

Language: Английский

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Cell-cell interaction in the pathogenesis of inherited retinal diseases

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: March 4, 2024

The retina is part of the central nervous system specialized for vision. Inherited retinal diseases (IRD) are a group clinically and genetically heterogenous disorders that lead to progressive vision impairment or blindness. Although each disorder rare, IRD accumulatively cause blindness in up 5.5 million individuals worldwide. Currently, pathophysiological mechanisms not fully understood there limited treatment options available. Most caused by degeneration light-sensitive photoreceptors. Genetic mutations abrogate structure and/or function photoreceptors visual followed loss In healthy retina, structurally functionally interact with pigment epithelium (RPE) Müller glia (MG) maintain homeostasis. Multiple photoreceptor as major phenotype RPE- MG-associated genes. Recent studies also reveal compromised MG RPE ubiquitously expressed ciliary Therefore, could be direct consequence gene secondary dysfunction their interaction partners retina. This review summarizes photoreceptor-RPE/MG supporting functions discusses how disruption these processes degeneration, an aim provide unique perspective pathogenesis paradigm. We will first describe biology then discuss between MG/RPE well implications disease pathogenesis. Finally, we summarize recent advances therapeutics targeting RPE.

Language: Английский

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Current Progress Regarding Cordyceps militaris, Its Metabolite Function, and Its Production

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(11), P. 4610 - 4610

Published: May 27, 2024

Cordyceps militaris is a valuable medicinal fungus which has been widely used as traditional medicine in East Asia. Compared to the well-known C. sinensis, can produce similar fermented metabolites with various biological activities, but it requires shorter culture time and simpler conditions, therefore, attracted increasing attention recent years. The purpose of this review was organize current studies regarding metabolite production from relative their functions. We combined findings correlate different fermentation modes obtain full view processes yield product. While research on not uncommon date, its high value still highlights importance developing more modern for industrial production.

Language: Английский

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Anti-Aging Potential of Bioactive Phytoconstituents Found in Edible Medicinal Plants: A Review

Sci, Journal Year: 2024, Volume and Issue: 6(2), P. 36 - 36

Published: June 13, 2024

Aging is a complex biological and physiological change that leads to loss of function in all living organisms. Although the mechanism behind aging process still largely unknown, scientific studies have shown oxidative stress age-related low autophagy, which are associated with various chronic diseases such as cancer, diabetes, cardiovascular diseases, neurodegenerative promote aging. Interestingly, many medicinal plants their biologically active compounds ability extend lifespan they can inhibit autophagy. This review evaluates provides up-to-date information on anti-aging potential bioactive edible plants. In this study, seventeen (17) from effects were reviewed. vivo vitro showed these exhibit via mechanisms activation increases antioxidant enzymes, reductions reactive oxygen species, inhibition inflammatory markers, downregulation senescence genes. study suggests containing may health lifespan. However, exact mechanisms, effective doses, clinical trials, genotoxic agents should be further investigated.

Language: Английский

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Association between dietary inflammatory index with all-cause and cardiovascular disease mortality among older US adults: A longitudinal cohort study among a nationally representative sample

Archives of Gerontology and Geriatrics, Journal Year: 2023, Volume and Issue: 118, P. 105279 - 105279

Published: Nov. 21, 2023

Language: Английский

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Elevating the significance of legume intake: A novel strategy to counter aging‐related mitochondrial dysfunction and physical decline

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(3)

Published: April 18, 2024

Mitochondrial dysfunction increasingly becomes a target for promoting healthy aging and longevity. The of mitochondria with age ultimately leads to decline in physical functions. Among them, biogenesis the imbalances metabolism reactive oxygen species as signaling organelles process have aroused our attention. Dietary intervention mitochondrial during processes is essential, greater attention should be directed toward healthful legume intake. Legumes are constantly under investigation their nutritional bioactive properties, consumption may yield antiaging mitochondria-protecting benefits. This review summarizes age, discusses benefits legumes on function, introduces potential role managing aging-related decline. Additionally, it reveals intake elderly offers viable approach developing legume-based functional food.

Language: Английский

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