Comparison of the Therapeutic Efficacy and Autophagy-Mediated Mechanisms of Action of Urine-Derived and Adipose-Derived Stem Cells in Osteoarthritis DOI
Tianhao Xu, Kaibo Zhang, Yunan Hu

et al.

The American Journal of Sports Medicine, Journal Year: 2024, Volume and Issue: 52(12), P. 3130 - 3146

Published: Sept. 23, 2024

Background: Osteoarthritis (OA) is a prevalent and disabling disease that affects significant proportion of the global population. Urine-derived stem cells (USCs) have shown great prospects in treatment OA, but there no study has compared them with traditional cells. Purpose: This aimed to compare therapeutic efficacy mechanisms USCs adipose-derived (ADSCs) for OA treatment. Study Design: Controlled laboratory study. Methods: We biological properties ADSCs using CCK-8, colony formation, EdU, adhesion, apoptosis assays. evaluated protective effects on IL-1β–treated chondrocytes by chemical staining, immunofluorescence, Western blotting. assessed chondrocyte autophagy transmission electron microscopy, also intra-articular injections gross, histological, micro–computed tomography, immunohistochemical analyses an rat model induced anterior cruciate ligament transection. Results: showed higher proliferation, DNA synthesis, anti-apoptotic abilities than ADSCs. Both increased expression cartilage-specific proteins decreased matrix degradation–related inflammatory factors chondrocytes. had greater advantage suppressing MMP-13 enhanced chondrocytes, being more effective The inhibitor 3-MA reduced Conclusion: To our knowledge, this first explore knee Considering superior terms noninvasive acquisition, high cost-benefit ratio, low ethical concerns, suggests they may be promising option under rigorous regulatory pathways. Clinical Relevance: cell source treat strengthens evidence application USCs.

Language: Английский

Emerging roles of exosomes in oral diseases progression DOI Creative Commons
Jiayi Wang, Junjun Jing, Chenchen Zhou

et al.

International Journal of Oral Science, Journal Year: 2024, Volume and Issue: 16(1)

Published: Jan. 15, 2024

Abstract Oral diseases, such as periodontitis, salivary gland and oral cancers, significantly challenge health conditions due to their detrimental effects on patient’s digestive functions, pronunciation, esthetic demands. Delayed diagnosis non-targeted treatment profoundly influence patients’ prognosis quality of life. The exploration innovative approaches for early detection precise represents a promising frontier in medicine. Exosomes, which are characterized nanometer-sized extracellular vesicles, secreted by virtually all types cells. As the research continues, complex roles these intracellular-derived vesicles biological processes have gradually unfolded. Exosomes attracted attention valuable diagnostic therapeutic tools ability transfer abundant cargos intricate involvement multiple cellular functions. In this review, we provide an overview recent applications exosomes within field focusing inflammation-related bone diseases squamous cell carcinomas. We characterize exosome alterations demonstrate potential biomarkers diagnosis, highlighting indicators diseases. also summarize targeted therapy proposed future directions use clinical treatment.

Language: Английский

Citations

23
Unveiling the Wonders of Bacteria-Derived Extracellular Vesicles: From Fundamental Functions to Beneficial Applications DOI Creative Commons

Mariam Rima,

Mariam Dakramanji,

E. Hayek

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42509 - e42509

Published: Feb. 1, 2025

:Extracellular vesicles (EVs), are critical mediators of intercellular communication and exhibit significant potential across various biomedical domains. These nano-sized, membrane-encapsulated entities have captured substantial interest due to their diverse roles in pathogenesis promising therapeutic applications. EVs manage numerous physiological processes by transferring bioactive molecules, including proteins, lipids, nucleic acids, between cells. This review delves into the factors influencing properties EVs, such as temperature stress conditions, which collectively influence size, composition, functional attributes. We also describe emerging emphasizing involvement microbial interactions, immune modulation, antimicrobial resistance spread innovative diagnostic instruments. Despite applications, advancement EV-based therapies faces several challenges, will be discussed. By elucidating these elements, we aim provide a comprehensive overview transformative revolutionizing diagnostics therapeutics medicine.

Language: Английский

Citations

4
Nrf2 activation: a key mechanism in stem cell exosomes-mediated therapies DOI Creative Commons
Zeinab Vahidinia,

Abolfazl Azami Tameh,

Shirin Barati

et al.

Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)

Published: March 2, 2024

Abstract Exosomes are nano-sized membrane extracellular vesicles which can be released from various types of cells. originating inflammatory or injured cells have detrimental effects on recipient cells, while exosomes derived stem not only facilitate the repair and regeneration damaged tissues but also inhibit inflammation provide protective against diseases, suggesting they may serve as an alternative strategy transplantation. a fundamental role in communication between through transfer proteins, bioactive lipids nucleic acids (like miRNAs mRNAs) This significantly impacts both physiological pathological functions Nuclear factor erythroid 2–related 2 (Nrf2), transcription factor, is able to mitigate damage caused by oxidative stress signaling pathways. The positive resulting activation Nrf2 pathway different disorders been documented literature. Studies confirmed that could act effective agonists. However, limited studies explored therapeutic cell-derived exosomes. review provides comprehensive overview existing knowledge concerning pathways impact exerted cell some common diseases. Graphical

Language: Английский

Citations

8
Mesenchymal Stem Cell-Derived Exosomes as a Treatment Option for Osteoarthritis DOI Open Access

Anupama Vadhan,

Tanvi Gupta, Wen‐Li Hsu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9149 - 9149

Published: Aug. 23, 2024

Osteoarthritis (OA) is a leading cause of pain and disability worldwide in elderly people. There critical need to develop novel therapeutic strategies that can effectively manage improve the quality life for older Mesenchymal stem cells (MSCs) have emerged as promising cell-based therapy age-related disorders due their multilineage differentiation strong paracrine effects. Notably, MSC-derived exosomes (MSC-Exos) gained significant attention because they recapitulate MSCs into benefits without causing any associated risks compared with direct cell transplantation. These help transport bioactive molecules such proteins, lipids, nucleic acids, which influence various cellular processes related tissue repair, regeneration, immune regulation. In this review, we provided an overview MSC-Exos considerable treatment option osteoarthritis. This review will go over underlying mechanisms by may alleviate pathological hallmarks OA, cartilage degradation, synovial inflammation, subchondral bone changes. Furthermore, summarized current preclinical evidence highlighted results from vitro vivo studies, well progress clinical trials using treat OA.

Language: Английский

Citations

8
Engineered MSC‐sEVs as a Versatile Nanoplatform for Enhanced Osteoarthritis Treatment via Targeted Elimination of Senescent Chondrocytes and Maintenance of Cartilage Matrix Metabolic Homeostasis DOI Creative Commons
Kai Feng, Jiashuo Liu,

Liangzhi Gong

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 4, 2025

Abstract Chondrocyte senescence is an important pathogenic factor causing osteoarthritis (OA) progression through persistently producing pro‐inflammatory factors. Mesenchymal stem cells‐derived small extracellular vesicles (MSC‐sEVs) have shown anti‐inflammatory effects in OA models, while persistent existence of senescent chondrocytes still promotes cartilage destruction. Therefore, improving the targeted elimination ability on required to facilitate translation MSC‐sEVs treatment. In this study, versatile engineered are developed targetedly clear and maintain metabolic homeostasis. Specifically, loaded with siRNA mouse double minute 2 homologue (siMDM2) modified cartilage‐targeting peptide WYRGRL‐PEG 2K ‐DSPE (WPD), named WPD‐sEVs siMDM2 . The results demonstrate modification improves cellular uptake chondrocytes, thus antiaging effects. Importantly, multifunctional enhances penetration extends joint retention time MSC‐sEVs. both post‐traumatic mice naturally aged mice, more effectively eliminates maintained matrix By using P53 phosphorylation inhibitor, essential role MDM2‐P53 pathway function verified. ex vivo cultured human explants, it confirmed that alleviates phenotype. Altogether, findings suggest promising translational potential for

Language: Английский

Citations

1
Extracecellulr vesicles (EVs) microRNAs (miRNAs) derived from mesenchymal stem cells (MSCs) in osteoarthritis (OA); detailed role in pathogenesis and possible therapeutics DOI Creative Commons
Seyede Sara Pakdaman Kolour,

Saeide Nematollahi,

Masoud Dehbozorgi

et al.

Heliyon, Journal Year: 2025, Volume and Issue: unknown, P. e42258 - e42258

Published: Jan. 1, 2025

Language: Английский

Citations

1
Exosomes derived from MSC as drug system in osteoarthritis therapy DOI Creative Commons
Shuzhan Wen, Xin Huang,

Jingchun Ma

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: March 20, 2024

Osteoarthritis (OA) is the most common degenerative disease of joint with irreversible cartilage damage as main pathological feature. With development regenerative medicine, mesenchymal stem cells (MSCs) have been found to strong therapeutic potential. However, intraarticular MSCs injection therapy limited by economic costs and ethics. Exosomes derived from MSC (MSC-Exos), important intercellular communication mode MSCs, contain nucleic acid, proteins, lipids, microRNAs, other biologically active substances. excellent editability specificity, MSC-Exos function a targeted delivery system for OA treatment, modulating immunity, inhibiting apoptosis, promoting regeneration. This article reviews mechanism action in treatment osteoarthritis, current research status preparation its application drug therapy.

Language: Английский

Citations

7
Mesenchymal stem cells for cartilage regeneration: Insights into molecular mechanism and therapeutic strategies DOI
Merlin Mamachan, Khan Sharun, S. Amitha Banu

et al.

Tissue and Cell, Journal Year: 2024, Volume and Issue: 88, P. 102380 - 102380

Published: April 10, 2024

Language: Английский

Citations

7
Efficiently directing differentiation and homing of mesenchymal stem cells to boost cartilage repair in osteoarthritis via a nanoparticle and peptide dual-engineering strategy DOI
Cuixi Wu,

Zhenwen Huang,

Jianmao Chen

et al.

Biomaterials, Journal Year: 2024, Volume and Issue: 312, P. 122720 - 122720

Published: July 27, 2024

Language: Английский

Citations

7
Mesenchymal stem cell–derived extracellular vesicles in joint diseases: Therapeutic effects and underlying mechanisms DOI Creative Commons

Jinhui Wu,

Jiangyi Wu, Zheng Liu

et al.

Journal of Orthopaedic Translation, Journal Year: 2024, Volume and Issue: 48, P. 53 - 69

Published: July 27, 2024

Joint diseases greatly impact the daily lives and occupational functioning of patients globally. However, conventional treatments for joint have several limitations, such as unsatisfatory efficacy side effects, necessitating exploration more efficacious therapeutic strategies. Mesenchymal stem cell (MSC)-derived EVs (MSC-EVs) demonstrated high efficacyin tissue repair regeneration, with low immunogenicity tumorigenicity. Recent studies reported that EVs-based therapy has considerable effects against diseases, including osteoarthritis, tendon ligament injuries, femoral head osteonecrosis, rheumatoid arthritis. Herein, we review potential various types MSC-EVs in aforementioned summarise mechanisms underlying specific biological MSC-EVs, discuss future prospects basic research on MSC-EV-based modalities their clinical translation. In general, this provides an in-depth understanding well mechanisms, which may be beneficial to translation treatment. The translational article: cell-free can effectively promote regeneration repair. When used treat desirable preclinical research. This supplement further treatment its

Language: Английский

Citations

6