The class III peroxidase gene family is involved in ascorbic acid induced delay of internal browning in pineapple DOI Creative Commons

Xiaowan Hou,

Zhiwei Lü, Keqian Hong

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 3, 2022

Excessive production of reactive oxygen species (ROS) leads to potential toxicity in an organism. Class III peroxidases (PRXs) play important role maintaining ROS homeostasis plants. Internal browning (IB) limits industrial development pineapple, which is the third most fruit trade world. IB mainly caused by ROS, and mechanism underlying still unknown from perspective ROS. Here, we soaked pineapples ascorbic acid after harvest before storage decrease excessive polyphenol oxidase (PPO) activity, ultimately restraining spread deterioration IB. Using phylogenetic analysis; identified 78 pineapple PRX genes (AcPRXs) divided them into five subgroups. Gene structure analysis indicated that exon numbers ranged 2 14, conserved motif verified all AcPRXs here have standard peroxidase domains. Analysis duplication events suggested tandem segmental may played equal roles expanding AcPRX family. Comprehensive transcriptomic uncovered negatively regulating occurrence In summary, found scavenging delayed occurrence. The results characterized family revealed responded growth development, regulated stage. This research provides target for future in-depth molecular mechanisms contributes develop IB-resistant varieties.

Language: Английский

Understanding AP2/ERF Transcription Factor Responses and Tolerance to Various Abiotic Stresses in Plants: A Comprehensive Review DOI Open Access
Ziming Ma,

Lanjuan Hu,

Wenzhu Jiang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 893 - 893

Published: Jan. 11, 2024

Abiotic stress is an adverse environmental factor that severely affects plant growth and development, plants have developed complex regulatory mechanisms to adapt these unfavourable conditions through long-term evolution. In recent years, many transcription families of genes been identified regulate the ability respond abiotic stresses. Among them, AP2/ERF (APETALA2/ethylene responsive factor) family a large class plant-specific proteins response stresses can also play role in regulating development. This paper reviews structural features classification factors are involved transcriptional regulation, reciprocal proteins, downstream genes, hormone-dependent signalling hormone-independent pathways stress. The synergise with hormone form cross-regulatory networks tolerance Many activate expression stress-responsive dependent or independent abscisic acid ethylene ethylene. addition, gibberellin, auxin, brassinosteroid, cytokinin-mediated responses. study interacting as well identification their target provide us more comprehensive understanding mechanism action stress, which improve plants' tolerate theoretical basis for increasing yield under

Language: Английский

Citations

63
Genome-wide characterization and expression analysis of Erf gene family in cotton DOI Creative Commons
Muhammad Mubashar Zafar, Abdul Rehman, Abdul Razzaq

et al.

BMC Plant Biology, Journal Year: 2022, Volume and Issue: 22(1)

Published: March 22, 2022

AP2/ERF transcription factors are important in a variety of biological activities, including plant growth, development, and responses to biotic abiotic stressors. However, little study has been done on cotton's genes, although cotton is an essential fibre crop. We were able examine the tissue expression patterns genes genome-wide basis because recently published whole genome sequence cotton. Genome-wide analysis ERF gene family within two diploid species (G. arboreum & G. raimondii) tetraploid barbadense, hirsutum) was performed.A total 118, 120, 213, 220 containing single AP2 domain identified arboreum, raimondii, barbadense hirsutum respectively. The unevenly distributed across 13/26 chromosomes A D genomes Synteny collinearity revealed that segmental duplications may have played crucial roles expansion family, as well tandem minor role. Cis-acting elements promoter sites Ghi-ERFs predict involvement multiple hormone stresses. Transcriptome qRT-PCR Ghi-ERF-2D.6, Ghi-ERF-12D.13, Ghi-ERF-6D.1, Ghi-ERF-7A.6 Ghi-ERF-11D.5 candidate against salinity tolerance upland cotton.Overwhelmingly, present paves way better understand evolution lays foundation for future investigation improving stress

Language: Английский

Citations

63
Transcriptomic Identification of Wheat AP2/ERF Transcription Factors and Functional Characterization of TaERF-6-3A in Response to Drought and Salinity Stresses DOI Open Access
Yang Yu,

Ming Yu,

Shuangxing Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(6), P. 3272 - 3272

Published: March 18, 2022

AP2/ERF (APETALA2/ethylene responsive factor) is a family of plant-specific transcription factors whose members are widely involved in many biological processes, such as growth, development, and biotic abiotic stress responses. Here, 20 genes were identified based on wheat RNA-seq data before after drought stress, classified AP2, ERF, DREB, RAV. The analysis gene structure revealed that about 85% had lost introns, which presumed to have been during the formation evolution genome. expression could be verified by qRT-PCR, further supported validity data. Subsequently, subcellular localization transcriptional activity experiments showed ERF proteins mainly located nucleus self-activating, supports their functions factors. Furthermore, we isolated novel induced drought, salt, cold stresses named it TaERF-6-3A. TaERF-6-3A overexpression increased sensitivity salt Arabidopsis, was physiological biochemical indices. Moreover, stress- antioxidant-related downregulated TaERF-6-3A-overexpressing plants. Overall, these results contribute understanding function wheat.

Language: Английский

Citations

44
Genome-wide identification and expression analysis of DREB family genes in cotton DOI Creative Commons
Jiuchang Su,

Shanglin Song,

Yi‐Ting Wang

et al.

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: March 30, 2023

Abstract Background Dehydration responsive element-binding ( DREB ) transcription factors are widely present in plants, and involve signalling transduction, plant growth development, stress response. genes have been characterized multiple species. However, only a few studied cotton, one of the most important fibre crops. Herein, genome‑wide identification, phylogeny, expression analysis family performed diploid tetraploid cotton Results In total, 193, 183, 80, 79 putative containing AP2 domain were identified using bioinformatics approaches G. barbadense , hirsutum arboretum raimondii respectively. Phylogenetic showed that based on categorization Arabidopsis genes, 535 divided into six subgroups (A1–A6) by MEGA 7.0. The distributed unevenly across 13/26 chromosomes A and/or D genomes. Synteny collinearity confirmed during evolution, whole genome duplications, segmental tandem duplications occurred then gene was further expanded. Further, evolutionary trees with conserved motifs, cis -acting elements, structure predicted, these results suggested might be involved hormone abiotic stresses responses. subcellular localization four species, proteins predominantly located nucleus. carried out real-time quantitative PCR, confirming response to early salinity osmotic stress. Conclusions Collectively, our presented comprehensive systematic understanding evolution demonstrated potential roles

Language: Английский

Citations

26
Overview of molecular mechanisms of plant leaf development: a systematic review DOI Creative Commons

Zhuo Lv,

Wanqi Zhao,

Shuxin Kong

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 7, 2023

Leaf growth initiates in the peripheral region of meristem at apex stem, eventually forming flat structures. Leaves are pivotal organs plants, serving as primary sites for photosynthesis, respiration, and transpiration. Their development is intricately governed by complex regulatory networks. encompasses five processes: leaf primordium initiation, polarity establishment, size expansion, shaping leaf, senescence. The primordia starts from side cone stem. Under precise regulation a series genes, establishes adaxial-abaxial axes, proximal-distal axes medio-lateral polarity, guides cells to divide differentiate specific direction, finally develops into leaves certain shape size. senescence kind programmed cell death that occurs it last stage development. Each these processes meticulously coordinated through intricate interplay among transcriptional factors, microRNAs, plant hormones. This review dedicated examining influences major factors hormones on developmental aspects leaves.

Language: Английский

Citations

20
Biotic and abiotic stress tolerance through CRISPR-Cas mediated genome editing DOI
Satabdi Ghosh, Gargee Dey

Journal of Plant Biochemistry and Biotechnology, Journal Year: 2022, Volume and Issue: 31(2), P. 227 - 238

Published: Jan. 7, 2022

Language: Английский

Citations

23
Genome-wide identification and bioinformatics analysis of the WRKY transcription factors and screening of candidate genes for anthocyanin biosynthesis in azalea (Rhododendron simsii) DOI Creative Commons
Cheng Wang, Dan Ye, Yan Li

et al.

Frontiers in Genetics, Journal Year: 2023, Volume and Issue: 14

Published: May 10, 2023

WRKY transcription factors have been demonstrated to influence the anthocyanin biosynthesis in many plant species. However, there is limited knowledge about structure and function of genes major ornamental azalea (Rhododendron simsii). In this study, we identified 57 RsWRKY R. simsii genome classified them into three main groups several subgroups based on their structural phylogenetic characteristics. Comparative genomic analysis suggested gene family has significantly expanded during evolution from lower higher Gene duplication indicated that expansion was primarily due whole-genome (WGD). Additionally, selective pressure (Ka/Ks) all pairs underwent purifying selection. Synteny 63 24 were orthologous Arabidopsis thaliana Oryza sativa, respectively. Furthermore, RNA-seq data used investigate expression patterns RsWRKYs, revealing 17 9 candidate may be associated with synthesis at bud full bloom stages, These findings provide valuable insights molecular mechanisms underlying Rhododendron species lay foundation for future functional studies genes.

Language: Английский

Citations

15
Genome-wide analysis of AP2/ERF gene and functional analysis of CqERF24 gene in drought stress in quinoa DOI
Xiaolin Zhu, Baoqiang Wang, Wenyu Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 127582 - 127582

Published: Oct. 21, 2023

Language: Английский

Citations

14
Genome-wide analysis of AP2/ERF transcription factors that regulate fruit development of Chinese prickly ash DOI Creative Commons
Лей Ма, Qianqian Shi,

Qin Ma

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: June 15, 2024

Abstract Background AP2/ERF is a large family of plant transcription factor proteins that play essential roles in signal transduction, growth and development, responses to various stresses. The has been identified verified by functional analysis plants, but so far there no comprehensive study these factors Chinese prickly ash. Phylogenetic, motif, analyses combined with transcriptome ash fruits at different developmental stages (30, 60, 90 days after anthesis) were conducted this study. Results 146 ZbAP2/ERF genes could be classified into 15 subgroups. motif revealed the presence motifs or elements each group may explain differences between groups. ZbERF13.2 , ZbRAP2-12 ZbERF2.1 showed high levels expression early fruit development. ZbRAP2-4 ZbERF3.1 significantly expressed coloring stage (R2 G2). ZbERF16 ripening level increased as continued develop. Relative gene 6 representative ZbAP2/ERFs assessed RT-qPCR agreed results. Conclusions These screening can used candidate affect results help guide future genetic improvement enrich our understanding their regulatory functions plants.

Language: Английский

Citations

5
Genome-Wide Identification and Characterization of AP2/ERF Transcription Factor Family Genes in Oil Palm under Abiotic Stress Conditions DOI Open Access
Lixia Zhou, Rajesh Yarra

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(6), P. 2821 - 2821

Published: March 10, 2021

The AP2/ERF transcription factor family members play crucial roles in controlling plant growth and development, as well responses to various abiotic stresses. Genome-wide identification characterization of genes has not yet been carried out the oil palm genome. In present work, we reported occurrence 172 EgAP2/ERFs (AP2, ERF, RAV & Soloist members) through genome-wide identification. Phylogenetic analysis was used divide them into four groups, including: 34 AP2, 131 5 RAV, 2 gene members. All were unevenly distributed across 16 chromosomes palm. Gene duplication elucidated tandem AP2/ERFs on chromosome blocks genome during evolution. structure conserved motif demonstrated nature intron/exon organization motifs among genes. Several cis-regulatory elements—related hormone, stress, defense responses—were identified promoter regions AP2/ERFs. Tissue-specific expression five different tissues also revealed by heatmap using available transcriptome data. Finally, stress (salinity, cold drought)-responsive validated qPCR analysis. Our study provided valuable information superfamily dissected their role conditions.

Language: Английский

Citations

29