BACH1 encourages ferroptosis by activating KDM4C‐mediated COX2 demethylation after cerebral ischemia–reperfusion injury DOI
Tianqi Zhang, Meimei Yang, Chi Ma

et al.

European Journal of Neuroscience, Journal Year: 2023, Volume and Issue: 58(1), P. 2194 - 2214

Published: May 11, 2023

Abstract It has been confirmed that BTB domain and CNC homologue 1 (BACH1) are involved in ferroptosis‐related diseases. However, the function of BACH1 cerebral ischemia–reperfusion injury (CIRI)‐induced ferroptosis remains to be largely unrevealed. First, analysis differentially expressed genes CIRI based on GEO dataset GSE119121 revealed was upregulated CIRI. level prominently increased middle artery occlusion (MCAO)/reperfusion model oxygen–glucose deprivation/reoxygenation cell model. Further, knock‐down markedly reduced iron ion concentration, ROS production, 4‐HNE lipid peroxidation levels facilitated GSH content, viability protein GPX4 SLC7A11, while an pcDNA‐KDM4C or pcDNA‐COX2 combined with siRNA could not enhance this effect. Mechanistically, bound KDM4C promoter transcriptionally activate its expression. Besides, occupy locus COX2 gene, promoting expression by eliminating H3K9me3. Overexpression overturned effects inhibition. In vivo findings displayed brain infraction, pathological damage neuronal loss rate MCAO mice were conspicuously decreased after knock‐down. This study reveals encourages neuroblastoma cells mouse tissues activating KDM4C‐mediated demethylation.

Language: Английский

The molecular mechanisms and potential drug targets of ferroptosis in myocardial ischemia–reperfusion injury DOI Creative Commons
Chen-Hua Zhang, Yujie Yan, Qi Luo

et al.

Life Sciences, Journal Year: 2024, Volume and Issue: 340, P. 122439 - 122439

Published: Jan. 24, 2024

Myocardial ischemia–reperfusion injury (MIRI), caused by the initial interruption and subsequent restoration of coronary artery blood, results in further damage to cardiac function, affecting prognosis patients with acute myocardial infarction. Ferroptosis is an iron-dependent, superoxide-driven, non-apoptotic form regulated cell death that involved pathogenesis MIRI. characterized accumulation lipid peroxides (LOOH) redox disequilibrium. Free iron ions can induce oxidative stress as a substrate Fenton reaction lipoxygenase (LOX) participate inactivation variety antioxidants including CoQ10 GPX4, destroying balance causing death. The metabolism amino acid, iron, lipids, associated pathways, considered specific hallmark ferroptosis. This review systematically summarizes latest research progress on mechanisms ferroptosis discusses analyzes therapeutic approaches targeting alleviate

Language: Английский

Citations

14
Exosomes from preconditioned mesenchymal stem cells: Tissue repair and regeneration DOI Creative Commons

Ruili Long,

Shuai Wang

Regenerative Therapy, Journal Year: 2024, Volume and Issue: 25, P. 355 - 366

Published: Feb. 14, 2024

As a prominent research area in tissue repair and regeneration, mesenchymal stem cells (MSCs) have garnered substantial attention for their potential the treatment of various diseases. It is now widely recognized that therapeutic effects MSCs primarily occur through paracrine mechanisms. Among these mechanisms, exosomes play crucial role by exerting series regulatory on surrounding tissues. While shown promise treating diseases, they do some limitations, such as limited secretion, poor targeting, single functionality. However, MSC preconditioning can enhance production exosomes, lead to more stable functionality improve effects. Moreover, could also serve carriers specific drugs or genes, enabling precise treatments This review summarizes most recent literatures how influences regenerative provides new insights into application derived from MSCs.

Language: Английский

Citations

14
Ferroptosis in organ ischemia–reperfusion injuries: recent advancements and strategies DOI

Xiaoyu Luan,

Peng Chen, Longyu Miao

et al.

Molecular and Cellular Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: March 31, 2024

Language: Английский

Citations

11
Mesenchymal stem cells and ferroptosis: Clinical opportunities and challenges DOI Creative Commons

Mengling Cui,

Fukun Chen,

Lishi Shao

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(3), P. e25251 - e25251

Published: Feb. 1, 2024

This review discusses recent experimental and clinical findings related to ferroptosis, with a focus on the role of MSCs. Therapeutic efficacy current applications MSC-based ferroptosis therapies are also discussed.

Language: Английский

Citations

10
Human spindle-shaped urine-derived stem cell exosomes alleviate severe fatty liver ischemia–reperfusion injury by inhibiting ferroptosis via GPX4 DOI Creative Commons

Shangheng Shi,

Cunle Zhu,

Shuzhen Shi

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 21, 2025

Severe hepatic steatosis can exacerbate Ischemia–reperfusion injury (IRI), potentially leading to early graft dysfunction and primary non-function. In this study, we investigated the heterogeneity of different subpopulations Urine-derived stem cells (USCs) explore most suitable cell subtype for treating severe steatotic liver IRI. This study utilized scRNA-seq Bulk RNA-seq investigate transcriptional between Spindle-shaped USCs (SS-USCs) Rice-shaped (RS-USCs). Additionally, rat fatty Liver transplantation (LT) model, mouse IRI Steatotic Hepatocyte Hypoxia-Reoxygenation (SHP-HR) model were constructed. Extracellular vesicles derived from SS-USCs RS-USCs isolated subjected mass spectrometry analysis. The therapeutic effects Exosomes (SS-USCs-Exo) (RS-USCs-Exo) explored, elucidating their potential mechanisms in inhibiting ferroptosis alleviating Multiple omics analyses confirmed that possess strong tissue repair antioxidant capabilities, while have differentiate towards specific directions such as kidney, nervous system, skeletal particularly showing great application renal system reconstruction. Further experiments demonstrated vivo vitro models confirming SS-USCs-Exo significantly inhibit alleviate IRI, whereas RS-USCs/RS-USCs-Exo are less pronounced. Analysis comparing proteomic differences RS-USCs-Exo revealed primarily improve cellular viability by secreting exosomes containing Glutathione Peroxidase 4 (GPX4) protein. highlights donor through presence GPX4 protein exosomes. appropriate

Language: Английский

Citations

1
Chronic cadmium exposure triggered ferroptosis by perturbing the STEAP3-mediated glutathione redox balance linked to altered metabolomic signatures in humans DOI
Ping Deng,

Jingdian Li,

Yonghui Lu

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 905, P. 167039 - 167039

Published: Sept. 15, 2023

Language: Английский

Citations

20
Natural Flavonoids and Ferroptosis: Potential Therapeutic Opportunities for Human Diseases DOI
Zheng Zhou,

Jiye Li,

Xiaochuan Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: unknown

Published: April 7, 2023

Flavonoids are a class of bioactive phytochemicals containing core 2-phenylchromone skeleton and widely found in fruits, vegetables, herbs. Such natural compounds have gained significant attention due to their various health benefits. Ferroptosis is recently discovered unique iron-dependent mode cell death. Unlike traditional regulated death (RCD), ferroptosis associated with excessive lipid peroxidation on cellular membranes. Accumulating evidence suggests that this form RCD involved variety physiological pathological processes. Notably, multiple flavonoids been shown be effective preventing treating diverse human diseases by regulating ferroptosis. In review, we introduce the key molecular mechanisms ferroptosis, including iron metabolism, several major antioxidant systems. Additionally, summarize promising targeting which provides novel ideas for management such as cancer, acute liver injury, neurodegenerative diseases, ischemia/reperfusion (I/R) injury.

Language: Английский

Citations

18
Implications of Crosstalk between Exosome-Mediated Ferroptosis and Diseases for Pathogenesis and Treatment DOI Creative Commons
Zixuan Zhou, Benshuai You, Cheng Ji

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(2), P. 311 - 311

Published: Jan. 13, 2023

Ferroptosis is a type of iron-dependent cell death caused by ferrous iron overload, reactive oxygen species generation through the Fenton reaction, and lipid peroxidation, leading to antioxidative system dysfunction and, ultimately, membrane damage. The functional role ferroptosis in human physiology pathology considered cause or consequence diseases. Circulating exosomes mediate intercellular communication organ crosstalk. They not only transport proteins nucleic acids derived from parental cells but also serve as vehicles for targeted delivery exogenous cargo. Exosomes regulate delivering biological material recipient cell, affecting ferroptosis-related proteins, transporting ferritin-bound out cell. This review discusses pathogenesis mediated endogenous therapeutic potential In addition, this explores exosome-mediated diseases with an emphasis on strategies engineering therapy.

Language: Английский

Citations

17
A recognition of exosomes as regulators of epigenetic mechanisms in central nervous system diseases DOI Creative Commons

Shunxin Hu,

Lei Feng,

Zhonghong Yang

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: March 11, 2024

Exosomes, vesicular structures originating from cells, participate in the conveyance of proteins and nucleic acids. Presently, centrality epigenetic modifications neurological disorders is widely acknowledged. Exosomes exert influence over various phenomena, thereby modulating post-transcriptional regulatory processes contingent upon their constituent makeup. Consequently, heightened attention directed toward exosomes as instigators alterations has burgeoned recent years. Notably, serve vehicles for delivering methyltransferases to recipient cells. More significantly, non-coding RNAs, particularly microRNAs (miRNAs), represent pivotal contents within exosomes, wielding capacity expression diverse factors cerebral milieu. The transfer these exosomal amidst brain encompassing neuronal cells microglia, assumes a critical role genesis progression disorders, also, this not limited it may deal with any human disease, such cancer, cardiovascular diseases. This review will concentrate on elucidating regulation exosome-induced events its subsequent ramifications A more profound comprehension involvement exosome-mediated contributes awareness etiology advancement afflictions.

Language: Английский

Citations

8
The crosstalk between exosomes and ferroptosis: a review DOI Creative Commons
Jiao Wu, Zhongyu Li, Yu Wu

et al.

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: April 9, 2024

Abstract Exosomes are a subtype of extracellular vesicles composed bioactive molecules, including nucleic acids, proteins, and lipids. generated by the fusion intracellular multivesicular bodies (MVBs) with cell membrane subsequently released into space to participate in intercellular communication diverse biological processes within target cells. As crucial mediator, exosomes have been implicated regulating ferroptosis—an iron-dependent programmed death characterized lipid peroxide accumulation induced reactive oxygen species. The involvement iron, lipid, amino acid metabolism contributes their regulatory role specific mechanisms underlying how modulate ferroptosis, which remains incompletely understood, some related studies still preliminary. Therefore, targeting regulation ferroptosis holds promise for future clinical treatment strategies across various diseases. This review aims provide insights pathophysiology governing interaction between implications disease development serve as reference further research.

Language: Английский

Citations

8