Frontiers in Cell and Developmental Biology,
Journal Year:
2021,
Volume and Issue:
8
Published: Jan. 12, 2021
Autophagy
is
an
intracellular
degradation
process
involved
in
the
removal
of
proteins
and
damaged
organelles
by
formation
a
double-membrane
vesicle
named
autophagosome
degraded
through
fusion
with
lysosomes.
An
intricate
relationship
between
autophagy
endosomal
exosomal
pathways
can
occur
at
different
stages
important
implications
for
normal
physiology
human
diseases.
Recent
researches
have
revealed
that
extracellular
vesicles
(EVs),
such
as
exosomes,
could
cytoprotective
role
inducing
autophagy;
on
other
hand,
plays
crucial
biogenesis
exosomes.
Although
importance
these
processes
cancer
well
established,
their
interplay
tumor
only
beginning
to
be
documented.
In
some
contexts
(1)
exosome-mediated
release
are
coordinately
activated,
sharing
molecular
machinery
regulatory
mechanisms;
(2)
cell-released
exosomes
impact
recipient
cells
mechanisms
yet
determined;
(3)
exosome-autophagy
affect
drug
resistance
microenvironment
(TME).
this
review,
we
survey
emerging
discoveries
relevant
crosstalk
context
initiation,
progression
recurrence.
Consequently,
discuss
clinical
targeting
autophagy-exosomal
pathway
interaction
how
lay
basis
purpose
novel
therapeutics.
Nature Communications,
Journal Year:
2020,
Volume and Issue:
11(1)
Published: April 29, 2020
Abstract
Small
extracellular
vesicles
called
exosomes
affect
multiple
autocrine
and
paracrine
cellular
phenotypes.
Understanding
the
function
of
requires
a
variety
tools,
including
live
imaging.
Our
previous
live-cell
reporter,
pHluorin-CD63,
allows
dynamic
subcellular
monitoring
exosome
secretion
in
migrating
spreading
cells.
However,
dim
fluorescence
inability
to
make
stably-expressing
cell
lines
limit
its
use.
We
incorporated
stabilizing
mutation
pHluorin
moiety,
M153R,
which
now
exhibits
higher,
stable
expression
cells
superior
secretion.
Using
this
improved
construct,
we
visualize
secreted
3D
culture
vivo
identify
role
for
promoting
leader–follower
behavior
2D
migration.
Incorporating
an
additional
non-pH-sensitive
red
fluorescent
tag
visualization
lifecycle,
multivesicular
body
(MVB)
trafficking,
MVB
fusion,
uptake
endosome
acidification.
This
reporter
will
be
useful
tool
understanding
both
roles
exosomes.
Leukemia,
Journal Year:
2020,
Volume and Issue:
34(12), P. 3126 - 3135
Published: Sept. 14, 2020
Abstract
There
are
concepts
in
science
that
need
time
to
overcome
initial
disbelief
before
finally
arriving
at
the
moment
when
they
embraced
by
research
community.
One
of
these
is
biological
meaning
small,
spheroidal
vesicles
released
from
cells,
which
described
literature
as
microparticles,
microvesicles,
or
exosomes.
In
beginning,
this
was
difficult,
it
hard
distinguish
small
cell
debris
apoptotic
bodies.
However,
may
represent
first
language
cell–cell
communication,
existed
a
more
specific
intercellular
cross-talk
between
ligands
and
receptors
emerged
during
evolution.
review
article,
we
will
use
term
“extracellular
microvesicles”
(ExMVs)
refer
blebs
different
sizes
surrounded
lipid
layer
membrane.
We
have
accepted
an
invitation
Editor-in-Chief
write
observance
20th
anniversary
2001
ASH
Meeting
our
team
demonstrated
that,
horizontal
transfer
several
bioactive
molecules,
including
mRNA
species
proteins,
ExMVs
harvested
embryonic
stem
cells
could
modify
hematopoietic
stem/progenitor
expand
them
ex
vivo.
Interestingly,
result
moved
ExMV
forward
published
2005
Leukemia
,
having
been
previously
rejected
other
major
scientific
journals
out
simple
disbelief.
Therefore,
best
judge
new
concept
passage
time,
although
speed
its
adoption
aided
perseverance
confidence
one’s
own
data.
perspective
provide
brief
update
on
current
status
of,
hopes
for,
likely
future
well
therapeutic
diagnostic
applications,
with
special
emphasis
hematopoiesis.
Cell Communication and Signaling,
Journal Year:
2022,
Volume and Issue:
20(1)
Published: April 12, 2022
Extracellular
vehicles
(EVs)
are
a
heterogeneous
group
of
cell
and
membranous
particles
originating
from
different
compartments.
EVs
participate
in
many
essential
physiological
functions
mediate
fetal-maternal
communications.
Exosomes
the
smallest
unit
EVs,
which
delivered
to
extracellular
space.
can
be
released
by
umbilical
cord,
placenta,
amniotic
fluid,
membranes
involved
angiogenesis,
endothelial
migration,
embryo
implantation.
Also,
various
diseases
such
as
gestational
hypertension,
diabetes
mellitus
(GDM),
preterm
birth,
fetal
growth
restriction
related
content
placental
exosomes
during
pregnancy.
Due
exosomes'
ability
transport
signaling
molecules
their
effect
on
sperm
function,
they
also
play
role
male
female
infertility.
In
new
insight,
exosomal
miRNA
diagnose
treat
infertilities
disorders.
this
review,
we
focused
Video
abstract.
BMC Cancer,
Journal Year:
2022,
Volume and Issue:
22(1)
Published: Jan. 10, 2022
Breast
cancer
is
the
most
common
cancer,
and
leading
cause
of
cancer-related
deaths,
among
females
world-wide.
Recent
research
suggests
that
extracellular
vesicles
(EVs)
play
a
major
role
in
development
breast
metastasis.
Axillary
lymph
node
dissection
(ALND)
procedure
patients
with
known
metastases,
after
surgery
large
amounts
serous
fluid
are
produced
from
axilla.
The
overall
aim
was
to
isolate
characterize
EVs
axillary
fluid,
more
specifically
determine
if
potential
biomarkers
could
be
identified.
Journal of Experimental & Clinical Cancer Research,
Journal Year:
2023,
Volume and Issue:
42(1)
Published: Feb. 15, 2023
Exosome
is
crucial
mediator
and
play
an
important
role
in
tumor
angiogenesis.
Tip
cell
formation
a
prerequisite
for
persistent
angiogenesis
which
causes
metastasis.
However,
the
functions
underlying
mechanisms
of
cell-derived
exosomes
tip
remain
less
understood.
Molecular Cancer,
Journal Year:
2024,
Volume and Issue:
23(1)
Published: Jan. 19, 2024
Abstract
The
production
and
release
of
tumor-derived
small
extracellular
vesicles
(TDSEVs)
from
cancerous
cells
play
a
pivotal
role
in
the
propagation
cancer,
through
genetic
biological
communication
with
healthy
cells.
TDSEVs
are
known
to
orchestrate
invasion-metastasis
cascade
via
diverse
pathways.
Regulation
early
metastasis
processes,
pre-metastatic
niche
formation,
immune
system
regulation,
angiogenesis
initiation,
matrix
(ECM)
remodeling,
modulation,
epithelial-mesenchymal
transition
(EMT)
among
pathways
regulated
by
TDSEVs.
MicroRNAs
(miRs)
carried
within
as
double-edged
sword
can
either
promote
or
inhibit
cancer
progression.
serve
excellent
markers
for
detection
tumors,
tumor
metastases.
From
therapeutic
point
view,
risk
may
be
reduced
limiting
On
other
hand,
represent
promising
approach
vivo
delivery
cargo
present
review
article
discusses
recent
developments
current
views
field
research
clinical
applications.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 27, 2025
Extracellular
vesicles
(EVs)
are
cell
derived
nanovesicles
which
implicated
in
both
physiological
and
pathological
intercellular
communication,
including
the
initiation,
progression,
metastasis
of
cancer.
The
exchange
biomolecules
between
stromal
cells
cancer
via
EVs
can
provide
a
window
to
monitor
development
real
time
for
better
diagnostic
interventional
strategies.
In
addition,
process
secretion
internalization
by
tumor
microenvironment
(TME)
be
exploited
delivering
therapeutics.
have
potential
targeted,
biocompatible,
efficient
delivery
platform
treatment
other
diseases.
Natural
as
well
engineered
nanomedicine
immense
disease
intervention.
Here,
we
an
overview
current
knowledge
EVs'
function
therapeutic
applications
setting,
EV
engineering
International Journal of Molecular Sciences,
Journal Year:
2020,
Volume and Issue:
22(1), P. 14 - 14
Published: Dec. 22, 2020
Exosomes
are
cell-secreted
nanovesicles
that
naturally
contain
biomolecular
cargoes
such
as
lipids,
proteins,
and
nucleic
acids.
mediate
intercellular
communication,
enabling
the
transfer
biological
signals
from
donor
cells
to
recipient
cells.
Recently,
exosomes
emerging
promising
drug
delivery
vehicles
due
their
strong
stability
in
blood
circulation,
high
biocompatibility,
low
immunogenicity,
natural
targeting
ability.
In
particular,
derived
specific
types
of
can
carry
endogenous
signaling
molecules
with
therapeutic
potential
for
cancer
treatment,
thus
presenting
a
significant
impact
on
targeted
therapy.
Furthermore,
be
engineered
display
moieties
surface
or
load
additional
agents.
Therefore,
comprehensive
understanding
exosome
biogenesis
development
efficient
engineering
techniques
will
provide
new
avenues
establish
convincing
clinical
strategies
based
exosomes.
This
review
focuses
applications
various
technologies
enable
accurate
target
