A high salt diet impairs the bladder epithelial barrier and activates the NLRP3 and NF‑κB signaling pathways to induce an overactive bladder in vivo DOI Open Access
Jingwen Xue, Zhipeng Zhou,

Zhangrui Zhu

et al.

Experimental and Therapeutic Medicine, Journal Year: 2024, Volume and Issue: 28(3)

Published: July 12, 2024

Overactive bladder (OAB) is a condition characterized by an urgency to urinate, which associated with the urodynamic observation of detrusor overexcitation. Although etiology OAB currently unclear, it has been suggested that in patients OAB, disruption epithelial barrier integrity can disturb normal contractile function detrusor. Additionally, dietary preferences have influence severity OAB. Therefore, aim present study was investigate effect high salt diet (HSD) on development murine model. Mice were fed either HSD or standard for 8 weeks, following voiding characteristics and assessed. The demonstrated mice OAB‑like symptoms such as increased urinary frequency non‑voiding contractions. group thinner mucus layer decreased expression markers, tight junction protein‑1 claudin‑1, may be potentially indicative induced damage. A weeks treatment at uroepithelium cellular (SV‑HUC‑1s) level resulted uroepithelial oxidative stress inflammatory cell infiltration, indicated levels TNF‑α IL‑1β, well activation nucleotide‑binding domain leucine‑rich‑containing family pyrin domain‑containing 3 (NLRP3) NF‑κB signaling pathways in vivo vitro. could important risk factor overactivation impairing NLRP3 pathways. Remodeling reduction response potential targets future.

Language: Английский

Microglial NLRP3 Inflammasomes in Alzheimer’s Disease Pathogenesis: From Interaction with Autophagy/Mitophagy to Therapeutics DOI
Gunel Ayyubova,

Leelavathi N. Madhu

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Language: Английский

Citations

1
A swift expanding trend of extracellular vesicles in spinal cord injury research: a bibliometric analysis DOI Creative Commons
Zhiguo Fan, Wu Ji,

Chen Shenyuan

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Aug. 23, 2023

Abstract Extracellular vesicles (EVs) in the field of spinal cord injury (SCI) have garnered significant attention for their potential applications diagnosis and therapy. However, no bibliometric assessment has been conducted to evaluate scientific progress this area. A search articles Web Science (WoS) from January 1, 1991, May 2023, yielded 359 papers that were analyzed using various online analysis tools. These cited 10,842 times with 30.2 per paper. The number publications experienced explosive growth starting 2015. China United States led research initiative. Keywords divided into 3 clusters, including “Pathophysiology SCI”, “Bioactive components EVs”, “Therapeutic effects EVs SCI”. By integrating average appearing year (AAY) keywords VoSviewer time zone map Citation Explosion CiteSpace, focal point undergone a transformative shift. emphasis moved away pathophysiological factors such as “axon”, “vesicle”, “glial cell” more mechanistic applied domains “activation”, “pathways”, “hydrogels” “therapy”. In conclusions, institutions are expected allocate resources towards EVs-loaded hydrogel therapy utilization innovative materials mitigation.

Language: Английский

Citations

22
Bidirectional two-sample Mendelian randomization analysis reveals a causal effect of interleukin-18 levels on postherpetic neuralgia risk DOI Creative Commons
Liang Xiao, Yuchao Fan

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: May 25, 2023

Postherpetic neuralgia (PHN) is a debilitating complication of herpes zoster, characterized by persistent neuropathic pain that significantly impairs patients' quality life. Identifying factors determine PHN susceptibility crucial for its management. Interleukin-18 (IL-18), pro-inflammatory cytokine implicated in chronic pain, may play critical role development.In this study, we conducted bidirectional two-sample Mendelian randomization (MR) analyses to assess genetic relationships and potential causal associations between IL-18 protein levels increasing risk, utilizing genome-wide association study (GWAS) datasets on these traits. Two obtained from the EMBL's European Bioinformatics Institute database which contained 21,758 individuals with 13,102,515 SNPs Complete GWAS summary data 3,394 5,270,646 SNPs. The dataset FinnGen biobank had 195,191 16,380,406 SNPs.Our findings two different suggest correlation genetically predicted elevations an increased PHN.(IVW, OR 95% CI: 2.26, 1.07 4.78; p = 0.03 2.15, 1.10 4.19; =0.03, respectively), potentially indicating effect risk. However, did not detect any liability risk levels.These new insights into identifying at developing aid development novel prevention treatment approaches PHN.

Language: Английский

Citations

17
Engineered exosomes as a prospective therapy for diabetic foot ulcers DOI Creative Commons

Lifei Guo,

Dan Xiao,

Helin Xing

et al.

Burns & Trauma, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 1, 2024

Abstract Diabetic foot ulcer (DFU), characterized by high recurrence rate, amputations and mortality, poses a significant challenge in diabetes management. The complex pathology involves dysregulated glucose homeostasis leading to systemic local microenvironmental complications, including peripheral neuropathy, micro- macro-angiopathy, recurrent infection, persistent inflammation re-epithelialization. Novel approaches accelerate DFU healing are actively pursued, with focus on utilizing exosomes. Exosomes natural nanovesicles mediating cellular communication containing diverse functional molecular cargos, DNA, mRNA, microRNA (miRNA), lncRNA, proteins, lipids metabolites. While some exosomes show promise modulating function promoting healing, their efficacy is limited low yield, impurities, loading content inadequate targeting. Engineering enhance curative activity represents potentially more efficient approach for DFUs. This could facilitate focused repair regeneration of nerves, blood vessels soft tissue after development. review provides an overview pathogenesis, strategies exosome engineering the targeted therapeutic application engineered addressing critical pathological changes associated

Language: Английский

Citations

8
A Review of Extracellular Vesicles in COVID‐19 Diagnosis, Treatment, and Prevention DOI Creative Commons
Peng Su, Yuchen Wu, Feng Xie

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(19)

Published: May 5, 2023

The 2019 novel coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) is ongoing, and has necessitated scientific efforts in diagnosis, treatment, prevention. Interestingly, extracellular vesicles (EVs) have been crucial these developments. EVs are a collection of various nanovesicles which delimited lipid bilayer. They enriched proteins, nucleic acids, lipids, metabolites, naturally released from different cells. Their natural material transport properties, inherent long-term recycling ability, excellent biocompatibility, editable targeting, inheritance parental cell properties make one the most promising next-generation drug delivery nanocarriers active biologics. During COVID-19 pandemic, many made to exploit payload for treatment COVID-19. Furthermore, strategies that use engineered manufacture vaccines neutralization traps produced efficacy animal experiments clinical trials. Here, recent literature on application damage repair, prevention reviewed. And therapeutic value, strategies, safety, biotoxicity production applications EV agents as well inspiration using block eliminate viruses discussed.

Language: Английский

Citations

14
Roles of the Caspase-11 Non-Canonical Inflammasome in Rheumatic Diseases DOI Open Access
Young‐Su Yi

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2091 - 2091

Published: Feb. 8, 2024

Inflammasomes are intracellular multiprotein complexes that activate inflammatory signaling pathways. comprise two major classes: canonical inflammasomes, which were discovered first and activated in response to a variety of pathogen-associated molecular patterns (PAMPs) danger-associated (DAMPs), non-canonical recently only lipopolysaccharide (LPS). Although larger number studies have successfully demonstrated particularly the NLRP3 inflammasome, play roles various rheumatic diseases, including rheumatoid arthritis (RA), infectious (IR), gouty (GA), osteoarthritis (OA), systemic lupus erythematosus (SLE), psoriatic (PA), ankylosing spondylitis (AS), Sjögren’s syndrome (SjS), regulatory such as mouse caspase-11 human caspase-4 these diseases still largely unknown. Interestingly, an increasing reported possible for inflammasomes pathogenesis models disease. This review comprehensively summarizes discusses recent emerging demonstrating focusing on progression types provides new insights into strategies developing potential therapeutics prevent treat well associated by targeting inflammasomes.

Language: Английский

Citations

5
Quercetin suppresses ovariectomy-induced osteoporosis in rat mandibles by regulating autophagy and the NLRP3 pathway DOI
Yue Xiong, Chengwei Huang, Chao Shi

et al.

Experimental Biology and Medicine, Journal Year: 2023, Volume and Issue: 248(23), P. 2363 - 2380

Published: Dec. 1, 2023

With the aging population and popularity of implant prostheses, an increasing number postmenopausal osteoporosis (PMOP) patients require restorations; however, poor bone condition affects long-term stability prostheses. This study aimed to investigate therapeutic effect quercetin (QR) compared with alendronate (ALN), primary treatment for PMOP, on mandibular (OP) induced by ovariectomy (OVX) in female rats. Adult rats were treated QR (50 mg/kg/day), ALN (6.25 mg/kg/week) gavage 8 weeks, chloroquine (CQ, 10 mg/kg/twice a week), cytokine release inhibitory drug 3 (MCC950, mg/kg/three times week) intraperitoneal injection weeks after bilateral OVX. Blood samples collected prior euthanasia; mandibles harvested subjected micro-computed tomography (micro-CT) pathological analysis. administration controlled weight gain significantly improved microstructure OVX rats, mass, mineral density (BMD), reducing trabecular spacing, decreasing osteoclast numbers. Western blotting, real-time quantitative PCR (RT-qPCR), serum markers confirmed that inhibited interleukin- 1β (IL-1β) interleukin-18 (IL-18) nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) protein (NLRP3) pathway thereby inhibiting differentiation, immunofluorescence western blotting also autophagy suppressed tartrate-resistant acid phosphatase (TRAP)-stained positive osteoclasts. The findings suggest may protect structure prevent loss osteoporotic NLRP3 osteoclasts comparable effects ALN, thus have potential be promising alternative supplement preventive PMOP.

Language: Английский

Citations

11
MCC950 Ameliorates Diabetic Muscle Atrophy in Mice by Inhibition of Pyroptosis and Its Synergistic Effect with Aerobic Exercise DOI Creative Commons
Xiaoyu Yan, Pengyu Fu, Yimin Zhang

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(3), P. 712 - 712

Published: Feb. 4, 2024

Diabetic muscle atrophy is an inflammation-related complication of type-2 diabetes mellitus (T2DM). Even though regular exercise prevents further deterioration atrophic status, there no effective mediator available for treatment and the underlying cellular mechanisms are less explored. In this study, we investigated therapeutic potential MCC950, a specific, small-molecule inhibitor NLRP3, to treat pyroptosis diabetic in mice. Furthermore, used MCC950 intervene protective effects aerobic against Blood gastrocnemius (GAS) samples were collected after 12 weeks intervention state was assessed. We initially corroborated phenotype db/db mice (D) by comparison with control m/m (W) examining parameters such as fasting blood glucose (D vs. W: 24.47 ± 0.45 mmol L−1 4.26 0.6 L−1, p < 0.05), grip strength 166.87 15.19 g 191.76 14.13 g, time 1082.38 104.67 s 1716 168.55 s, 0.05) speed exhaustion 24.25 2.12 m min−1 34.75 2.66 min−1, GAS wet weight 0.07 0.01 0.13 ratio body 0.18 0.01% 0.54 0.02%, fiber cross-sectional area (FCSA) 1875 368.19 µm2 2747.83 406.44 µm2, 0.05). found that both (10 mg kg−1) improved had deteriorated mice, inhibited serum inflammatory markers significantly attenuated GAS. addition, combined (DEI) exhibited improvement uptake capacity performance. This also FCSA indicated Laminin immunofluorescence compared group alone (DI) (DEI DI: 2597 310.97 1974.67 326.15 or only (DE) DE: 2006.33 263.468 Intriguingly, combination reduced NLRP3-mediated factors cleaved-Caspase-1, GSDMD-N prevented apoptosis These findings first demonstrate targeting improves homeostasis function. report inhibiting can enhance beneficial on atrophy. Since T2DM age-related diseases, young current study do not seem fully reflect characteristics Considering fragile nature complete implementation intervention, relatively thoroughly confirmed. Taken together, comprehensively effect mass, performance, well synergistic therefore providing novel strategy disease.

Language: Английский

Citations

4
Mesenchymal stem cell-derived extracellular vesicles for human diseases DOI Open Access
Xiaofang Zhang, Xiaofang Che, Sibo Zhang

et al.

Extracellular Vesicles and Circulating Nucleic Acids, Journal Year: 2024, Volume and Issue: 5(1), P. 64 - 82

Published: Feb. 6, 2024

Stem cell therapy is a novel approach for treating various severe and intractable diseases, including autoimmune disorders, organ transplants, tumors, neurodegenerative diseases. Nevertheless, the extensive utilization of stem cells constrained by potential tumorigenicity, challenges in precise differentiation, rejection concerns, ethical considerations. Extracellular vesicles possess ability to carry diverse bioactive factors from deliver them specific target or tissues. Moreover, they offer advantage low immunogenicity. Consequently, have facilitate therapeutic cells, mitigating risks associated with direct application. Therefore, use extracellular clinical diseases has received increasing attention. This review summarizes advances mesenchymal (MSC). MSC are used treatment inflammatory such as rheumatoid arthritis, liver injury, COVID-19, allergies; repair tissue damage heart disease, kidney osteoarthritic diseases; carrier tumors; regenerative agent disorders Alzheimer's Parkinson's.

Language: Английский

Citations

4
Quercetin-primed BMSC-derived extracellular vesicles ameliorate chronic liver damage through miR-136-5p and GNAS/STAT3 signaling pathways DOI

Xiaodan Jiang,

Zhejun Liu,

Hongjie You

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 142, P. 113162 - 113162

Published: Sept. 27, 2024

Language: Английский

Citations

4