Komagataeibacter oboediens changes outer membrane vesicle-associated activities after exposure on the International Space Station DOI Open Access

Olga Podolich,

Ganna Zubova,

I. V. Orlovska

et al.

Biopolymers and Cell, Journal Year: 2023, Volume and Issue: 39(2), P. 131 - 145

Published: June 26, 2023

Outer membrane vesicles (OMVs) carry various biomolecule cargo, including enzymes, and communicate with the surrounding via them.The role of enzyme-associated OMVs from food-grade bacteria in human health disease is not clear, particular, after impact stressful factors (changed gravity atmosphere, UV-radiation) context astronauts' diet.Aim.We researched Komagataeibacter oboediens isolated kombucha microbial community samples exposed to space/Mars-like stressors simulated on International Space Station cultivated within a five-year period know more about their behaviour biomolecules mammalian cells.Methods.A series vitro experiments RNA cleavage, DNA transcription inhibition cytotoxic assay OMVs/ were conducted.SignalP 6.0 was used for detecting signal peptides predicted ribonucleases K. genome, ProtComp Version 9 served identifying RNase sub-cellular location.Results.The before an conditions differed, relying differences associated nucleolytic activity, inhibitory capabilities against T7 bacteriophage polymerase Taq polymerases effect, despite identical nucleotide sequences homologous genes.The transcriptions less pronounced than ground-based strain.It correlated lower activity loss cytotoxicity towards malignant cells, even five years flight.Bacterial I be located OMV periplasm.Conclusion.Komagataeibacter's OMV-associated activities modified exposure inherited non-genetic manner.

Language: Английский

Identifying size-dependent toxin sorting in bacterial outer membrane vesicles DOI Creative Commons
Aarshi N. Singh,

Justin B. Nice,

Angela C. Brown

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 3, 2023

Abstract Gram-negative bacteria produce outer membrane vesicles (OMVs) that play a critical role in cell-cell communication and virulence. Despite being isolated from single population of bacteria, OMVs can exhibit heterogeneous size toxin content, which be obscured by assays measure ensemble properties. To address this issue, we utilize fluorescence imaging individual to reveal size-dependent sorting. Our results showed the oral bacterium Aggregatibacter actinomycetemcomitans (A . ) produces with bimodal distribution, where larger were much more likely possess leukotoxin (LtxA). Among smallest (< 100 nm diameter), fraction are positive ranges 0-30%, while largest (> 200 diameter) between 70-100% positive. OMV method provides non-invasive way observe surface heterogeneity at nanoscale level determine size-based heterogeneities without need for separation.

Language: Английский

Citations

6
Salmonella enterica and outer membrane vesicles are current and future options for cancer treatment DOI Creative Commons
Genesy Pérez Jorge, Marco Túlio Pardini Gontijo, Marcelo Brocchi

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Dec. 5, 2023

Conventional cancer therapies have many limitations. In the last decade, it has been suggested that bacteria-mediated immunotherapy may circumvent restrictions of traditional treatments. For example, Salmonella enterica is most promising bacteria for treating due to its intrinsic abilities, such as killing tumor cells, targeting, penetrating, and proliferating into tumor. S. genetically modified ensure safety increase antitumor efficacy. This bacterium used a vector delivering anticancer agents combination therapy with chemotherapy, radiotherapy, or photothermic. Recent studies reported efficacy outer membrane vesicles (OMVs) derived from . OMVs are considered safer than attenuated can stimulate immune system they comprise immunogens found on surface their parent bacteria. Furthermore, also be nanocarriers agents. review describes advances in against mechanisms by which fights cancer. We highlight use innovative strategies requiring further investigation.

Language: Английский

Citations

5
Muropeptides and muropeptide transporters impact on host immune response DOI Creative Commons
María Lucía Orsini Delgado, João G. Magalhães,

Rachel Morra

et al.

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Oct. 22, 2024

In bacteria, the cell envelope is key element surrounding and protecting bacterial content from mechanical or osmotic damages. It allows selective interchanges of solutes, ions, cellular debris, drugs between compartments external environment, thanks to presence transmembrane proteins called transporters. The major component peptidoglycan, consisting long linear glycan strands cross-linked by short peptide stems. During growth under stress conditions, peptidoglycan fragments, muropeptides, are released bacteria recognized host Pattern Recognition Receptor, promoting activation their innate defense mechanisms. review sums up salient aspects microbiota-host interaction with a focus on NOD-dependent immune response accountability muropeptide transporters in crosstalk antibiotic resistance. Furthermore, it retraces discoveries applications microorganisms-derived components such as vaccines vaccine adjuvants.

Language: Английский

Citations

1
Size-exclusion chromatography for the characterization of urinary extracellular vesicles DOI

S U Park,

Iqbal Jalaludin, Hyojin Hwang

et al.

Journal of Chromatography B, Journal Year: 2023, Volume and Issue: 1228, P. 123828 - 123828

Published: July 17, 2023

Language: Английский

Citations

3
Study of the Molecular Mechanisms of the Therapeutic Properties of Extracellular Vesicles DOI Open Access
Д. Н. Силачев

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7093 - 7093

Published: April 12, 2023

Extracellular vesicles (EVs) are small biological structures that released by cells and have important roles in intercellular communication [...].

Language: Английский

Citations

2
Potential therapeutic application and mechanism of gut microbiota-derived extracellular vesicles in polycystic ovary syndrome DOI Open Access

Liangliang Yang,

Tingxiu Liu,

Yan Liao

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 180, P. 117504 - 117504

Published: Sept. 27, 2024

Language: Английский

Citations

0
The Therapeutic Potential of Exosome Therapy in Sepsis Management: Addressing Complications and Improving Outcomes” DOI

Payam Ali-Khiavi,

Mahya Mohammadi,

Sajjad Masoumi

et al.

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 3, 2024

Language: Английский

Citations

0
Emerging role of gut microbiota extracellular vesicle in neurodegenerative disorders and insights on their therapeutic management DOI Creative Commons
Adil Hassan, Yin Huang,

Mahjabina

et al.

iMetaOmics., Journal Year: 2024, Volume and Issue: 1(2)

Published: Oct. 25, 2024

Abstract Targeting gut flora to lower sickness risk is a growing scientific subject. The intricate network of microorganisms (gut microbiota) in the human intestines regulates many physiological systems and may be important for general health. Recent research has shown dynamic relationship between microbiota central nervous system (CNS). Dysbiosis key establishing progressing diseases, including neurodegenerative disorders. Recently, extracellular vesicles (GMEVs) have been suggested as brain‐gut communication carriers. Vesicle components contact immune receptors, initiating neuroinflammatory responses causing diseases. This study seeks explain how its cause or worsen We also highlighted recent advances our understanding these GMEVs' cargo's routes, which could used drug delivery treatments. examines current state potential therapeutic effects GMEVs on illnesses.

Language: Английский

Citations

0
Correction: Emerging role of microbiota derived outer membrane vesicles to preventive, therapeutic and diagnostic proposes DOI Creative Commons
Saba Jalalifar,

Hassan Morovati Khamsi,

Seyed Reza Hosseini‐Fard

et al.

Infectious Agents and Cancer, Journal Year: 2023, Volume and Issue: 18(1)

Published: Feb. 2, 2023

Language: Английский

Citations

0
Komagataeibacter oboediens changes outer membrane vesicle-associated activities after exposure on the International Space Station DOI Open Access

Olga Podolich,

Ganna Zubova,

I. V. Orlovska

et al.

Biopolymers and Cell, Journal Year: 2023, Volume and Issue: 39(2), P. 131 - 145

Published: June 26, 2023

Outer membrane vesicles (OMVs) carry various biomolecule cargo, including enzymes, and communicate with the surrounding via them.The role of enzyme-associated OMVs from food-grade bacteria in human health disease is not clear, particular, after impact stressful factors (changed gravity atmosphere, UV-radiation) context astronauts' diet.Aim.We researched Komagataeibacter oboediens isolated kombucha microbial community samples exposed to space/Mars-like stressors simulated on International Space Station cultivated within a five-year period know more about their behaviour biomolecules mammalian cells.Methods.A series vitro experiments RNA cleavage, DNA transcription inhibition cytotoxic assay OMVs/ were conducted.SignalP 6.0 was used for detecting signal peptides predicted ribonucleases K. genome, ProtComp Version 9 served identifying RNase sub-cellular location.Results.The before an conditions differed, relying differences associated nucleolytic activity, inhibitory capabilities against T7 bacteriophage polymerase Taq polymerases effect, despite identical nucleotide sequences homologous genes.The transcriptions less pronounced than ground-based strain.It correlated lower activity loss cytotoxicity towards malignant cells, even five years flight.Bacterial I be located OMV periplasm.Conclusion.Komagataeibacter's OMV-associated activities modified exposure inherited non-genetic manner.

Language: Английский

Citations

0