Nose-on-Chip Nanobiosensors for Early Detection of Lung Cancer Breath Biomarkers

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(9), P. 4469 - 4494

Published: Sept. 9, 2024

Lung cancer remains a global health concern, demanding the development of noninvasive, prompt, selective, and point-of-care diagnostic tools. Correspondingly, breath analysis using nanobiosensors has emerged as promising noninvasive nose-on-chip technique for early detection lung through monitoring diversified biomarkers such volatile organic compounds/gases in exhaled breath. This comprehensive review summarizes state-of-the-art breath-based diagnosis employing chemiresistive-module supported by theoretical findings. It unveils fundamental mechanisms biological basis biomarker generation associated with cancer, technological advancements, clinical implementation nanobiosensor-based analysis. explores merits, challenges, potential alternate solutions implementing these settings, including standardization, biocompatibility/toxicity analysis, green sustainable technologies, life-cycle assessment, scheming regulatory modalities. highlights nanobiosensors' role facilitating precise, real-time, on-site leading to improved patient outcomes, enhanced management, remote personalized monitoring. Additionally, integrating biosensors artificial intelligence, machine learning, Internet-of-things, bioinformatics, omics technologies is discussed, providing insights into prospects intelligent sniffing nanobiosensors. Overall, this consolidates knowledge on breathomic biosensor-based screening, shedding light its significance applications advancing medical diagnostics reduce burden hospitals save human lives.

Language: Английский

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The metabolic characteristics and changes of chondrocytes in vivo and in vitro in osteoarthritis

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: May 24, 2024

Osteoarthritis (OA) is an intricate pathological condition that primarily affects the entire synovial joint, especially hip, hand, and knee joints. This results in inflammation synovium osteochondral injuries, ultimately causing functional limitations joint dysfunction. The key mechanism responsible for maintaining articular cartilage function chondrocyte metabolism, which involves energy generation through glycolysis, oxidative phosphorylation, other metabolic pathways. Some studies have shown chondrocytes OA exhibit increased glycolytic activity, leading to elevated lactate production decreased matrix synthesis. In cartilage, display alterations mitochondrial such as ATP stress, can contribute deterioration. Chondrocyte metabolism also anabolic processes extracellular substrate generation. During OA, undergo considerable changes different aspects, homeostasis Numerous been carried out provide tangible therapies by using various models vivo vitro targeting although there are still certain limitations. With growing evidence indicating essential role of disease etiology, this literature review explores characteristics presence both . To insight into complex reprogramming crucial during progression, we investigate dynamic interaction between pathways, lipid function. addition, highlights prospective future research directions novel approaches diagnosis treatment. Adopting a multifaceted strategy, our aims offer comprehensive understanding intricacies within with ultimate goal identifying therapeutic targets capable modulating treatment OA.

Language: Английский

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Pro-inflammatory cytokines in stress-induced depression: Novel insights into mechanisms and promising therapeutic strategies

Progress in Neuro-Psychopharmacology and Biological Psychiatry, Journal Year: 2024, Volume and Issue: 131, P. 110931 - 110931

Published: Jan. 2, 2024

Language: Английский

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Dynamic GelMA/DNA Dual‐Network Hydrogels Promote Woven Bone Organoid Formation and Enhance Bone Regeneration

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 23, 2025

Abstract Bone organoids, in vitro models mimicking native bone structure and function, rely on 3D stem cell culture for self‐organization, differentiation, ECM secretion, biomineralization, ultimately forming mineralized collagen hierarchies. However, their development is often limited by the lack of suitable matrices with optimal mechanical properties sustained growth differentiation. To address this, a dynamic DNA/Gelatin methacryloyl (GelMA) hydrogel (CGDE) developed to recapitulate key biochemical features ECM, providing supportive microenvironment organoid formation. This dual‐network engineered through hydrogen bonding between DNA GelMA, combined GelMA network crosslinking, resulting appropriate strength enhanced viscoelasticity. During 21‐day culture, CGDE facilitates cellular migration promoting woven (WBO) formation via intramembranous ossification. These WBOs exhibit spatiotemporal architectures supporting mineralization tissue remodeling. In vivo studies demonstrate that CGDE‐derived self‐adaptive properties, enabling rapid osseointegration within 4 weeks. work highlights as robust scalable platform development, offering new insights into biology innovative strategies regeneration.

Language: Английский

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From gut to liver: Exploring the crosstalk between gut-liver axis and oxidative stress in metabolic dysfunction-associated steatotic liver disease

Annals of Hepatology, Journal Year: 2025, Volume and Issue: unknown, P. 101777 - 101777

Published: Jan. 1, 2025

Non-alcoholic fatty liver disease (NAFLD), now recognized as metabolic dysfunction-associated steatotic (MASLD), represents a significant and escalating global health challenge. Its prevalence is intricately linked to obesity, insulin resistance, other components of the syndrome. As our comprehension MASLD deepens, it has become evident that this condition extends beyond liver, embodying complex, multi-systemic with hepatic manifestations mirror broader landscape. This comprehensive review delves into critical interplay between gut-liver axis oxidative stress, elucidating their pivotal roles in etiology progression MASLD. Our analysis reveals several key findings: (1) Bile acid dysregulation can trigger stress through enhanced ROS production hepatocytes Kupffer cells, leading mitochondrial dysfunction lipid peroxidation; (2) Gut microbiota dysbiosis disrupts intestinal barrier function, allowing increased translocation endotoxins like LPS, which activate inflammatory pathways TLR4 signaling promote via NADPH oxidase activation; (3) The redox-sensitive transcription factors NF-κB Nrf2 serve crucial mediators axis, regulating responses orchestrating antioxidant defenses; (4) Oxidative stress-induced damage function creates destructive feedback loop, further exacerbating inflammation progression. These findings highlight complex interrelationship pathogenesis, suggesting potential therapeutic targets for management.

Language: Английский

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Antioxidant and Anti-Inflammatory Effects of Vanillic Acid in Human Plasma, Human Neutrophils, and Non-Cellular Models In Vitro

Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 467 - 467

Published: Jan. 22, 2025

Vanillic acid (VA) is a dietary benzoic derivative, flavoring agent, and food stabilizer. In this study, the antioxidant anti-inflammatory potential of VA was explored in vitro ex vivo human immune cells non-cellular models. neutrophils, significantly downregulated fMLP-induced oxidative burst generation reactive oxygen species (ROS); it also suppressed release pro-inflammatory cytokines (TNF-α, IL-8) tissue-remodeling enzyme elastase-2 (ELA-2) stimulated with LPS fMLP+cytochalasin B. Additionally, showed good biocompatibility neutrophils peripheral blood mononuclear (PBMCs) across tested concentrations 1–50 µg/mL. Furthermore, at 1–5 μg/mL enhanced non-enzymatic capacity plasma (NEAC) prevented nitrative damage to proteins by protecting tyrosine moieties thiols from peroxynitrite. inhibited lipid peroxidation formation thiobarbituric acid-reactive substances (at 50 μg/mL) protein-bound carbonyls 5–50 peroxynitrite-treated plasma. tests, acted as hypochlorous hydrogen peroxide scavenger protein glycation, outperforming references Trolox aminoguanidine. Along existing data animal models studies on polyphenol intake, these results might support synergic role protection against chronic diseases related stress inflammation.

Language: Английский

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Hydrogels for Peripheral Nerve Repair: Emerging Materials and Therapeutic Applications

Gels, Journal Year: 2025, Volume and Issue: 11(2), P. 126 - 126

Published: Feb. 9, 2025

Peripheral nerve injuries pose a significant clinical challenge due to the complex biological processes involved in repair and their limited regenerative capacity. Despite advances surgical techniques, conventional treatments, such as autografts, are faced with limitations like donor site morbidity inconsistent functional outcomes. As such, there is growing interest new, novel, innovative strategies enhance regeneration. Tissue engineering/regenerative medicine its use of biomaterials an emerging example strategy. Within realm tissue engineering, functionalized hydrogels have gained considerable attention ability mimic extracellular matrix, support cell growth differentiation, even deliver bioactive molecules that can promote repair. These be engineered incorporate factors, peptides, stem cells, creating conducive microenvironment for cellular axonal Recent advancements materials well biology led development sophisticated hydrogel systems, not only provide structural support, but also actively modulate inflammation, recruitment, stimulate neurogenesis. This review explores potential peripheral repair, highlighting composition, biofunctionalization, mechanisms action. A comprehensive analysis preclinical studies provides insights into efficacy these promoting growth, neuronal survival, regeneration, and, ultimately, recovery. Thus, this aims illuminate promise transformative tool field bridging gap between complexity feasibility.

Language: Английский

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Inflammatory Microenvironment-Responsive Nanomicelles for Acute Lung Injury Therapy: ROS-Scavenging and Macrophage Repolarization

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101622 - 101622

Published: Feb. 27, 2025

Language: Английский

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Oxidative Stress, Gut Microbiota, and Extracellular Vesicles: Interconnected Pathways and Therapeutic Potentials

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3148 - 3148

Published: March 28, 2025

Oxidative stress (OS) and gut microbiota are crucial factors influencing human health, each playing a significant role in the development progression of chronic diseases. This review provides comprehensive analysis complex interplay between these two factors, focusing on how an imbalance reactive oxygen species (ROS) antioxidants leads to OS, disrupting cellular homeostasis contributing range conditions, including metabolic disorders, cardiovascular diseases, neurological cancer. The microbiota, diverse community microorganisms residing gastrointestinal tract, is essential for regulating immune responses, pathways, overall health. Dysbiosis, composition, closely associated with inflammation, dysfunction, various highlights influences influenced by complicating pathophysiology many conditions. Furthermore, emerging evidence has identified extracellular vesicles (EVs) as critical facilitators crosstalk OS microbiota. EVs also play signaling host tissues, modulating processes. function holds promise targeted therapies aimed at restoring microbial balance mitigating OS. Personalized therapeutic approaches, probiotics, antioxidants, fecal transplantation-based strategies, can be used address OS-related diseases improve health outcomes. Nonetheless, further research needed study molecular mechanisms underlying interactions potential innovative interventions offer novel strategies managing enhancing

Language: Английский

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Functional nanomaterials as photosensitizers or delivery systems for antibacterial photodynamic therapy

Biomaterials Advances, Journal Year: 2024, Volume and Issue: 159, P. 213820 - 213820

Published: Feb. 28, 2024

Language: Английский

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