Small extracellular vesicles – A host for advanced bioengineering and “Trojan Horse” of non-coding RNAs DOI Creative Commons
Artur Bryja, Łukasz Zadka,

Maryam Farzaneh

et al.

Life Sciences, Journal Year: 2023, Volume and Issue: 332, P. 122126 - 122126

Published: Sept. 27, 2023

Small extracellular vesicles (sEVs) are a type of membranous that can be released by cells into the space. The relationship between sEVs and non-coding RNAs (ncRNAs) is highly intricate interdependent. This symbiotic plays pivotal role in facilitating intercellular communication holds profound implications for myriad biological processes. concept their ncRNA cargo as "Trojan Horse" highlights remarkable capacity to traverse barriers surreptitiously deliver target cells, evading detection host-immune system. Accumulating evidence suggests may harnessed carriers ferry therapeutic ncRNAs capable selectively silencing disease-driving genes, particularly conditions such cancer. approach presents several advantages over conventional drug delivery methods, opening up new possibilities targeted therapy improved treatment outcomes. However, utilization agents raises valid concerns regarding possibility unforeseen consequences unintended impacts emerge from application. It important consider fundamental attributes ncRNAs, including an in-depth analysis practical clinical potentials exosomes, serving representative model encapsulating ncRNAs.

Language: Английский

Drug Delivery System Targeting Cancer-Associated Fibroblast for Improving Immunotherapy DOI Creative Commons

Zhongsong Zhang,

Rong Wang, Long Chen

et al.

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 483 - 503

Published: Jan. 1, 2025

Abstract: Cancer-associated fibroblasts (CAFs) are a heterogeneous population of non-malignant cells that play crucial role in the tumor microenvironment, increasingly recognized as key contributors to cancer progression, metastasis, and treatment resistance. So, targeting CAFs has always been considered an important part immunotherapy. However, improve efficacy therapy is currently major challenge. Nanomaterials show their unique advantages whole process. At present, nanomaterials have achieved significant accomplishments medical applications, particularly field cancer-targeted therapy, showing enormous potential. It confirmed can not only directly target CAFs, but also interact with microenvironment (TME) immune affect tumorigenesis. As for treatment, could enhance therapeutic effect many ways. Therefore, this review, we first summarized current understanding complex interactions between TME, cells, cells. Next, discussed common modern medicine respective impacts on tumors. Finally, focus application nano drug delivery system therapy. Keywords: cancer-associated fibroblasts, delivery, nanomedicine, immunotherapy

Language: Английский

Citations

3
Nanomaterials in cancer immunotherapy: targeting cancer-associated fibroblasts DOI Creative Commons

Zhongsong Zhang,

Long Chen

Cancer Nanotechnology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 17, 2025

Emphasizing the significance of cancer-associated fibroblasts (CAFs), non-malignant yet pivotal players within tumor microenvironment (TME), this review illuminates role inflammatory subtype (iCAF) as catalysts in cancer proliferation, metastasis, and therapeutic resistance. Given their paramount importance, targeting CAFs emerges a robust strategy evolving landscape immunotherapy. Nanomaterials, distinguished by unique features malleability, hold considerable promise biomedicine, especially precision-oriented domain therapy. Their aptitude for modulating immune responses, amplifying drug efficacy through precise delivery, discerningly focusing on cells TME situates nanomaterials formidable tools to transcend boundaries set conventional treatments. This scrutinizes convoluted interplay among CAFs, cells, TME. It further showcases widely utilized management. We underscore potential nanoscale delivery systems directed at underscoring transformative power revolutionizing therapies, enhancing precision, culminating improved patient outcomes.

Language: Английский

Citations

2
Extracellular Vesicles and Intercellular Communication: Challenges for In Vivo Molecular Imaging and Tracking DOI Creative Commons
Debora Petroni,

C. Fabbri,

Serena Babboni

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(6), P. 1639 - 1639

Published: June 1, 2023

Extracellular vesicles (EVs) are a heterogeneous class of cell-derived membrane released by various cell types that serve as mediators intercellular signaling. When into circulation, EVs may convey their cargo and intermediaries for intracellular communication, reaching nearby cells possibly also distant organs. In cardiovascular biology, activated or apoptotic endothelial (EC-EVs) disseminate biological information at short long distances, contributing to the development progression disease related disorders. The significance EC-EVs cell-cell communication has advanced, but thorough knowledge role intercommunication plays in healthy vascular is still lacking. Most data on derive from vitro studies, there little reliable available biodistribution specific homing vivo tissues. Molecular imaging techniques crucial monitoring networks both basal pathological circumstances. This narrative review provides an overview EC-EVs, trying highlight messengers interaction homeostasis disease, describes emerging applications modalities visualization vivo.

Language: Английский

Citations

23
Goat milk extracellular vesicles: immuno-modulation effects on porcine monocyte-derived macrophages in vitro DOI Creative Commons
Giulia Franzoni, Samanta Mecocci,

Chiara Grazia De Ciucis

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: June 16, 2023

Extracellular vesicles (EVs) are nanometric-membrane-bound sub-cellular structures, which can be recovered from milk. Milk EVs have drawn increasing interest due to their potential biomedical applications, therefore it is important investigate impact on key immune cells, such as macrophages.In this work, the immunomodulatory effects of goat milk untreated (moMФ) and classically activated (moM1) porcine monocyte-derived macrophages were investigated using flow cytometry, ELISA, gene expression assays.These particles efficiently internalized by high doses (60 mg protein weight) triggered upregulation MHC I II DR moMФ, but not moM1. In exposure low (0.6 mg) mEVs enhanced IL10, EBI3, IFNB, whereas up-regulated several pro-inflammatory cytokines. These nanosized structures slightly modulated cytokine Accordingly, (protein) contents in culture supernatants moMФ mildly affected mEVs, promoted increased release TNF, IL-8, IL-1a, IL-1b, IL-1Ra, IL-6, IL-10, IL-12. The cytokines content moM1 was critically affected.Overall, our data support a clinical application these molecules: they polarized toward an M1-like phenotype, activation seemed controlled, prevent potentially pathological over-reaction stressors.

Language: Английский

Citations

12
How Nanotherapeutic Platforms Play a Key Role in Glioma? A Comprehensive Review of Literature DOI Creative Commons

Yongqing Yang,

Nianlan Cheng,

Qiao Luo

et al.

International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 3663 - 3694

Published: July 1, 2023

Abstract: Glioblastoma (GBM), a highly aggressive form of brain cancer, is considered one the deadliest cancers, and even with most advanced medical treatments, affected patients have poor prognosis. However, recent advances in nanotechnology offer promising avenues for development versatile therapeutic diagnostic nanoplatforms that can deliver drugs to tumor sites through blood-brain barrier (BBB). Despite these breakthroughs, use GBM therapy has been subject great controversy due concerns over biosafety nanoplatforms. In years, biomimetic gained unprecedented attention biomedical field. With advantages such as extended circulation times, improved immune evasion active targeting compared conventional nanosystems, bionanoparticles shown potential applications. this prospective article, we endeavor comprehensively review application bionanomaterials treatment glioma, focusing on rational design multifunctional facilitate BBB infiltration, promote efficient accumulation tumor, enable precise imaging, achieve remarkable suppression. Furthermore, discuss challenges future trends Through careful optimization nanoplatforms, researchers are paving way toward safer more effective therapies patients. The nanoplatform applications glioma avenue precision medicine, which could ultimately improve patient outcomes quality life. Graphical Keywords: nanomedicine, bionanotechnology, therapy, drug delivery system

Language: Английский

Citations

9
Harnessing the power of goat milk-derived extracellular vesicles for medical breakthroughs: A review DOI
Yiming Meng,

Jing Sun,

Guirong Zhang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 262, P. 130044 - 130044

Published: Feb. 8, 2024

Language: Английский

Citations

3
Goat milk extracellular vesicles: Separation comparison of natural carriers for theragnostic application DOI Creative Commons

Jessie Santoro,

Silvia Nuzzo, Monica Franzese

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(6), P. e27621 - e27621

Published: March 1, 2024

Goat milk is a complex biological fluid, which in addition to having high nutritional value, it an interesting source of extracellular vesicles (EVs). Despite the countless potential applications that they offer many fields, not easy compare different proposed systems, and this major limitation for real translatability these natural nanoplatforms theragnostic purposes. Thus, useful further investigate reproducible methods separate goat EVs. The choice but also preprocessing has immense impact on separation, quality, yield Here, we tested four protocols EVs from unpasteurised milk: two based differential ultracentrifugation (DUC) size-exclusion chromatography (SEC). Moreover, assessed approaches pre-treatment (acidification precipitation) facilitate protein discharge. To best our knowledge, similar comparison all performed raw never been published before. Therefore, enriched EV samples were successfully obtained using both DUC SEC. Taken together, results may be helpful obtain carriers future personalised medicine.

Language: Английский

Citations

2
Small extracellular vesicles – A host for advanced bioengineering and “Trojan Horse” of non-coding RNAs DOI Creative Commons
Artur Bryja, Łukasz Zadka,

Maryam Farzaneh

et al.

Life Sciences, Journal Year: 2023, Volume and Issue: 332, P. 122126 - 122126

Published: Sept. 27, 2023

Small extracellular vesicles (sEVs) are a type of membranous that can be released by cells into the space. The relationship between sEVs and non-coding RNAs (ncRNAs) is highly intricate interdependent. This symbiotic plays pivotal role in facilitating intercellular communication holds profound implications for myriad biological processes. concept their ncRNA cargo as "Trojan Horse" highlights remarkable capacity to traverse barriers surreptitiously deliver target cells, evading detection host-immune system. Accumulating evidence suggests may harnessed carriers ferry therapeutic ncRNAs capable selectively silencing disease-driving genes, particularly conditions such cancer. approach presents several advantages over conventional drug delivery methods, opening up new possibilities targeted therapy improved treatment outcomes. However, utilization agents raises valid concerns regarding possibility unforeseen consequences unintended impacts emerge from application. It important consider fundamental attributes ncRNAs, including an in-depth analysis practical clinical potentials exosomes, serving representative model encapsulating ncRNAs.

Language: Английский

Citations

4