Biting Midges (Diptera: Ceratopogonidae) as Vectors of Viruses DOI Creative Commons
Helge Kampen, Doreen Werner

Microorganisms, Journal Year: 2023, Volume and Issue: 11(11), P. 2706 - 2706

Published: Nov. 4, 2023

Biting midges of the genus Culicoides occur almost globally and can regionally seasonally reach high abundances. Most species are hematophagous, feeding on all groups vertebrates, including humans. In addition to being nuisance pests, they able transmit disease agents, with some viruses causing morbidity and/or mortality in ruminants, horses Despite their impact animal husbandry, public health tourism, knowledge biology ecology culicoid biting interactions ingested pathogens or symbiotic microorganisms is limited. Research challenging due unknown larval habitats, insects' tiny size, inability establish breed most laboratory laborious maintenance colonies few that be reared laboratory. Consequently, natural transmission has experimentally been demonstrated for while, others, only indirect evidence vector potential exists. experimental data available sonorensis C. nubeculosus, kept western-world insectaries. This contribution gives an overview important midge vectors, transmitted viruses, culicoid-borne viral diseases epidemiologies summarizes little between midges, microflora arboviruses.

Language: Английский

Impact of the microbiome on mosquito-borne diseases DOI Creative Commons

Huicheng Shi,

Xi Yu, Gong Cheng

et al.

Protein & Cell, Journal Year: 2023, Volume and Issue: 14(10), P. 743 - 761

Published: April 25, 2023

Abstract Mosquito-borne diseases present a significant threat to human health, with the possibility of outbreaks new mosquito-borne always looming. Unfortunately, current measures combat these such as vaccines and drugs are often either unavailable or ineffective. However, recent studies on microbiomes may reveal promising strategies fight diseases. In this review, we examine advances in our understanding effects both mosquito vertebrate We argue that microbiome can have direct indirect impacts transmission diseases, symbiotic microorganisms, particularly Wolbachia bacteria, showing potential for controlling Moreover, skin vertebrates plays role preferences, while gut has an impact progression humans. As researchers continue explore highlight some future directions field. Ultimately, better interplay between mosquitoes, their hosts, pathogens, mosquitoes hosts hold key preventing

Language: Английский

Citations

26
Simultaneous detection of dengue virus serotypes in a dual-serotype-detection nucleic acid based lateral flow assay DOI
Biswadeep Das, Sagnika Samal, Hamida Hamdi

et al.

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: 111(3), P. 116679 - 116679

Published: Jan. 6, 2025

Language: Английский

Citations

1
Comparative analysis of bacterial microbiota in Aedes aegypti (Diptera: Culicidae): insights from field and laboratory populations in Colombia DOI
Marcela Gómez, David R. Martinez, Nicolás Luna

et al.

Journal of Medical Entomology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Abstract Comparative studies of the microbiota in whole-body mosquitoes from natural populations and laboratory-reared specimens are scarce, particularly tropical countries like Colombia, where understanding microbial patterns is critical for effective disease control vector management. This study examines bacterial Aedes aegypti by comparing field-collected 3 Colombian regions (Southern Amazon, Central Andean region, Northern Caribbean coast) with laboratory strains (Rockefeller, Otanche, Tolima). These highly endemic dengue associated lineage 1 Ae. aegypti, known its elevated competence. Using next-generation sequencing 16S rRNA gene Illumina technology, we found that exhibited significantly higher alpha beta diversity compared to specimens. Field were enriched families such as Acetobacteraceae, Lactobacillaceae, Bacillaceae, while showed a greater abundance Enterobacteriaceae. Differential analysis revealed Acetobacter Bacillus predominated field mosquitoes, whereas Asaia, Cedacea, Chryseobacterium more common Notably, Pseudomonas Acinetobacter consistently abundant across all samples. Our findings suggest environmental factors, habitat diet, influence composition Colombia. Future research should further explore how these alongside genetic components, shape mosquito–microbiota interactions their implications transmission

Language: Английский

Citations

1
Harnessing engineered symbionts to combat concurrent malaria and arboviruses transmission DOI Creative Commons
W.R. Hu, Han Gao, Chunlai Cui

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 1, 2025

Concurrent malaria and arbovirus infections pose significant public health challenges in tropical subtropical regions, demanding innovative control strategies. Here, we describe a strategy that employs multifunctional engineered symbiotic bacteria to suppress concurrent transmission of parasites, dengue, Zika viruses by various vector mosquitoes. The bacterium Serratia AS1, which efficiently spreads through Anopheles Aedes populations, is simultaneously produce anti-Plasmodium anti-arbovirus effector proteins controlled selected blood-induced promoter. Laboratory outdoor field-cage studies show the strains effectively inhibit Plasmodium infection mosquitoes Our findings provide foundation for use as powerful tool combat diseases. Engineered with secretion multiple effectors (Dengue Zika) mosquitoes, providing promising these

Language: Английский

Citations

1
Immune Reactions of Vector Insects to Parasites and Pathogens DOI Creative Commons
Norman A. Ratcliffe, C.B. Mello, Helena Carla Castro

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(3), P. 568 - 568

Published: March 12, 2024

This overview initially describes insect immune reactions and then brings together present knowledge of the interactions vector insects with their invading parasites pathogens. It is a way introducing this Special Issue subsequent papers presenting latest details these in each particular group vectors. Hopefully, paper will fill void literature since brief descriptions immunity have now been brought one publication could form starting point for those interested new to important area. Descriptions are given on mosquitoes, blackflies, sandflies, tsetse flies, lice, fleas triatomine bugs. Cellular humoral defences described separately but emphasis made co-operation processes completed response. The also emphasises need great care extracting haemocytes study as appreciation fragile nature often overlooked non-sterile media, smearing techniques excessive centrifugation sometimes used. potential vital role eicosanoids instigation many discussed. Finally, priming system, mainly considered possible mechanism presented.

Language: Английский

Citations

8
Innate immunity in Aedes mosquitoes: from pathogen resistance to shaping the microbiota DOI Creative Commons
Bretta Hixson, Robin Y Chen, Nicolas Buchon

et al.

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1901)

Published: March 18, 2024

Discussions of host–microbe interactions in mosquito vectors are frequently dominated by a focus on the human pathogens they transmit (e.g. Plasmodium parasites and arboviruses). Underlying between vector its transmissible pathogens, however, is physiology an insect living interacting with world bacteria fungi including commensals, mutualists primary opportunistic pathogens. Here we review what known about associated mosquitoes, emphasis members Aedes genus. We explore reciprocal effects microbe mosquito, microbe. analyse roles bacterial fungal symbionts development, their competence, potential uses as biocontrol agents for paratransgenesis. compartments gut, uncovering regionalization immune effectors modulators, which create zones resistance tolerance host controls corrals microbial symbionts. examine anatomical patterning basally expressed antimicrobial peptides. Finally, relationships inducible peptides canonical signalling pathways, comparing contrasting current knowledge each pathway mosquitoes to model Drosophila melanogaster . This article part theme issue ‘Sculpting microbiome: how factors determine respond colonization’.

Language: Английский

Citations

8
Role of the Microbiome in Aedes spp. Vector Competence: What Do We Know? DOI Creative Commons
Qesya Rodrigues Ferreira, Fabian Fellipe Bueno Lemos, Matheus Nascimento Moura

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(3), P. 779 - 779

Published: March 17, 2023

Aedes aegypti and albopictus are the vectors of important arboviruses: dengue fever, chikungunya, Zika, yellow fever. Female mosquitoes acquire arboviruses by feeding on infected host blood, thus being able to transmit it their offspring. The intrinsic ability a vector infect itself pathogen is known as competence. Several factors influence susceptibility these females be arboviruses, such activation innate immune system through Toll, immunodeficiency (Imd), JAK-STAT pathways, interference specific antiviral response pathways RNAi. It also believed that presence non-pathogenic microorganisms in microbiota arthropods could this response, provides baseline system, which may generate resistance against arboviruses. In addition, microbiome has direct action mainly due Wolbachia spp. block viral genome replication, added competition for resources within mosquito organism. Despite major advances area, studies still needed evaluate profiles competence, well further exploration individual roles components activating system.

Language: Английский

Citations

15
Innovative strategies and challenges mosquito-borne disease control amidst climate change DOI Creative Commons
Yuan Zhang, Minhao Wang, Mingxia Huang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 5, 2024

The revival of the transmission dynamics mosquito-borne diseases grants striking challenges to public health intensified by climate change worldwide. This inclusive review article examines multidimensional strategies and linked epidemiology such as malaria, dengue, Zika, chikungunya, yellow fever. It delves into how biology, pathogenic dynamics, vector distribution mosquitoes are influenced continuously rising temperatures, modified rainfall patterns, extreme climatic conditions. We also highlighted high likelihood malaria in Africa, dengue Southeast Asia, blowout Aedes North America Europe. Modern predictive tools developments surveillance, including molecular gears, Geographic Information Systems (GIS), remote sensing have boosted our capacity predict epidemics. Integrated data management techniques models based on conditions provide a valuable understanding planning. Based recent expert ideas, objective this is thoughtful existing landscape upcoming directions control regarding changing climate. determines emerging innovative ensure health.

Language: Английский

Citations

6
Bacillus thuringiensis Cyt Proteins as Enablers of Activity of Cry and Tpp Toxins against Aedes albopictus DOI Creative Commons
Liliana Lai,

Maite Villanueva,

Ane Muruzabal-Galarza

et al.

Toxins, Journal Year: 2023, Volume and Issue: 15(3), P. 211 - 211

Published: March 10, 2023

Aedes albopictus is a species of mosquito, originally from Southeast Asia, that belongs to the Culicidae family and Dipteran insect order. The distribution this vector has rapidly changed over past decade, making most temperate territories in world vulnerable important human vector-borne diseases such as dengue, yellow fever, zika or chikungunya. Bacillus thuringiensis var. israeliensis (Bti)-based insecticides represent realistic alternative common synthetic for control mosquito larvae. However, several studies have revealed emerging resistances major Bti Crystal proteins Cry4Aa, Cry4Ba Cry11Aa, finding new toxins necessary diminish exposure same toxicity factors overtime. Here, we characterized individual activity Cyt1Aa, Cry11Aa against A. found protein, Cyt1A-like, increases more than 20-fold. Additionally, demonstrated Cyt1A-like facilitates three toxins: Cry53-like, Cry56A-like Tpp36-like. All all, these results provide alternatives currently available products populations position Cyt enablers otherwise non-active crystal proteins.

Language: Английский

Citations

11
Insecticidal effects of Sargassum vulgare and Caulerpa racemosa extracts on Aedes aegypti DOI

Katiane Cruz Magalhães Xavier,

Dayane Kelly Dias do Nascimento Santos, Rômulo Carlos Dantas da Cruz

et al.

Parasitology International, Journal Year: 2025, Volume and Issue: 106, P. 103031 - 103031

Published: Jan. 13, 2025

Language: Английский

Citations

0