Advances in regenerative therapy: A review of the literature and future directions

Regenerative Therapy, Journal Year: 2020, Volume and Issue: 14, P. 136 - 153

Published: Feb. 21, 2020

There is enormous global anticipation for stem cell-based therapies that are safe and effective. Numerous pre-clinical studies present encouraging results on the therapeutic potential of different cell types including tissue derived cells. Emerging evidences in fields research suggest several safe, whereas their application effectiveness remain challenged. Multiple factors influence treatment outcomes proposed immunocompatibility potency, owing to variations origin, ex-vivo methodologies preparation handling This communication gives an overview literature data cells potentially promising regenerative therapy. As a case point, recent trends development mesenchymal (MSCs) therapy considered detail. MSCs can be isolated from variety tissues organs human body bone marrow, adipose, synovium, perinatal tissues. However, MSC products sources exhibit unique or varied levels abilities. The review finally focuses adipose tissue-derived (ASCs), with properties such as easier accessibility abundance, excellent proliferation differentiation capacities, low immunogenicity, immunomodulatory many other trophic properties. suitability ASCs, strategies improve innate capacities general highlighted among others.

Language: Английский

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Exosome-functionalized magnesium-organic framework-based scaffolds with osteogenic, angiogenic and anti-inflammatory properties for accelerated bone regeneration

Bioactive Materials, Journal Year: 2022, Volume and Issue: 18, P. 26 - 41

Published: Feb. 18, 2022

Language: Английский

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Exosomes from adipose-derived stem cells alleviate the inflammation and oxidative stress via regulating Nrf2/HO-1 axis in macrophages

Free Radical Biology and Medicine, Journal Year: 2021, Volume and Issue: 165, P. 54 - 66

Published: Jan. 18, 2021

Language: Английский

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Stem cell-derived exosomes: emerging therapeutic opportunities for wound healing

Stem Cell Research & Therapy, Journal Year: 2023, Volume and Issue: 14(1)

Published: April 26, 2023

Abstract Wound healing is a dynamic and highly sequential process involving series of overlapping spatial temporal phases, including hemostasis, inflammation, proliferation, tissue remodeling. Mesenchymal stem cells (MSCs) are multipotent with self-renewal, multidirectional differentiation potential, paracrine regulation. Exosomes subcellular vesicular components 30–150 nm in size novel carriers intercellular communication regulating the biological behaviors skin cells. Compared to MSCs, MSC-derived exosomes (MSC-exos) possess lower immunogenicity, easy storage, effective activity. MSC-exos, mainly derived from adipose-derived (ADSCs), bone marrow-derived MSCs (BMSCs), human umbilical cord (hUC-MSCs), other cell types, play role shaping activity fibroblasts, keratinocytes, immune cells, endothelial diabetic wounds, inflammatory wound repair, even wound-related keloid formation. Therefore, this study focuses on specific roles mechanisms different MSC-exos healing, as well current limitations various perspectives. Deciphering properties crucial providing promising cell-free therapeutic tool for cutaneous regeneration. Graphical

Language: Английский

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Human acellular amniotic membrane incorporating exosomes from adipose-derived mesenchymal stem cells promotes diabetic wound healing

Stem Cell Research & Therapy, Journal Year: 2021, Volume and Issue: 12(1)

Published: April 29, 2021

Abstract Background Diabetic wounds threaten the health and quality of life patients their treatment remains challenging. ADSC-derived exosomes have shown encouraging results in enhancing diabetic wound healing. However, how to use effectively is a problem that needs be addressed at present. Methods A mouse skin model was established. (ADSC-Exos) were isolated, vitro application evaluated using human umbilical vein endothelial cells (HUVECs) dermal fibroblasts (HDFs). After preparation characterization scaffold acellular amniotic membrane (hAAM) loaded with ADSC-Exos vitro, they transplanted into vivo healing phenomena observed by histological immunohistochemical analyses identify mechanism exosome-hAAM composites. Results The hAAM dressing very suitable for delivery exosomes. enhanced proliferation migration HDFs promoted tube formation HUVECs vitro. In from showed hAAM-Exos accelerated regulating inflammation, stimulating vascularization, promoting production extracellular matrix. Conclusion Exosome-incorporated scaffolds great potential healing, while also providing strong evidence future clinical applications

Language: Английский

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An Update on Adipose‐Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity

Advanced Science, Journal Year: 2023, Volume and Issue: 10(20)

Published: May 10, 2023

Abstract Over the last decade, adipose‐derived stem cells (ADSCs) have attracted increasing attention in field of regenerative medicine. ADSCs appear to be most advantageous cell type for therapies owing their easy accessibility, multipotency, and active paracrine activity. This review highlights current challenges translating ADSC‐based into clinical settings discusses novel strategies overcome limitations ADSCs. To further establish as an emerging platform medicine, this also provides update on advancements field, including fat grafting, wound healing, bone regeneration, skeletal muscle repair, tendon reconstruction, cartilage cardiac nerve regeneration. are expected more tissue‐specific increasingly important

Language: Английский

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Adipose Mesenchymal Stem Cell Derived Exosomes Promote Keratinocytes and Fibroblasts Embedded in Collagen/Platelet‐Rich Plasma Scaffold and Accelerate Wound Healing

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(40)

Published: June 21, 2023

Abstract Engineered skin substitutes derived from human significantly reduce inflammatory reactions mediated by foreign/artificial materials and are consequently easier to use for clinical application. Type I collagen is a main component of the extracellular matrix during wound healing has excellent biocompatibility, platelet‐rich plasma can be used as initiator cascade. Adipose mesenchymal stem cell exosomes crucial tissue repair play key roles in enhancing regeneration, promoting angiogenesis, regulating inflammation, remodeling matrix. Herein, plasma, which provide natural supports keratinocyte fibroblast adhesion, migration, proliferation, mixed form stable 3D scaffold. added scaffold improve performance engineered skin. The physicochemical properties this cellular analyzed, effect evaluated full‐thickness defect mouse model. reduces level inflammation promotes proliferation angiogenesis accelerate healing. Proteomic analysis shows that exhibit anti‐inflammatory proangiogenic effects collagen/platelet‐rich scaffolds. proposed method provides new therapeutic strategy theoretical basis regeneration repair.

Language: Английский

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Application of stem cells in regeneration medicine

MedComm, Journal Year: 2023, Volume and Issue: 4(4)

Published: June 17, 2023

Abstract Regeneration is a complex process affected by many elements independent or combined, including inflammation, proliferation, and tissue remodeling. Stem cells class of primitive with the potentiality differentiation, regenerate self‐replication, multidirectional immunomodulatory functions. their cytokines not only inextricably linked to regeneration ectodermal skin tissues, but also can be used for treatment variety chronic wounds. produce exosomes in paracrine manner. cell play an important role regeneration, repair, accelerated wound healing, biological properties which are similar stem cells, while safer more effective. Skin bone tissues critical organs body, essential sustaining life activities. The weak repairing ability leads pronounced impact on quality patients, could alleviated treatment. However, there obstacles that trough improved bioavailability. This paper summarizes applications mechanisms healing. We propose new ways utilizing through different nanoformulations, liposomes nanoliposomes, polymer micelles, microspheres, hydrogels, scaffold microneedles, improve use healing regeneration.

Language: Английский

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Extracellular Vesicles as Therapeutic Resources in the Clinical Environment

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2344 - 2344

Published: Jan. 25, 2023

The native role of extracellular vesicles (EVs) in mediating the transfer biomolecules between cells has raised possibility to use them as therapeutic vehicles. development therapies based on EVs is now expanding rapidly; here we will describe current knowledge different key points regarding a clinical setting. These are related cell sources EVs, isolation, storage, and delivery methods, well modifications releasing for improved production EVs. Finally, depict application trials, considering impact COVID-19 pandemic these therapies, pointing out that although it promising therapy human diseases, still initial phase its patients.

Language: Английский

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Extracellular Vesicles and Viruses: Two Intertwined Entities

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(2), P. 1036 - 1036

Published: Jan. 5, 2023

Viruses share many attributes in common with extracellular vesicles (EVs). The cellular machinery that is used for EV production, packaging of substrates and secretion also commonly manipulated by viruses replication, assembly egress. can increase production or manipulate EVs to spread their own genetic material proteins, while play a key role regulating viral infections transporting immunomodulatory molecules antigens initiate antiviral immune responses. Ultimately, the interactions between are highly interconnected, which has led interesting discoveries associated roles progression different diseases, as well new promise combinational therapeutics. In this review, we summarize relationships discuss major developments from past five years engineering virus-EV therapies.

Language: Английский

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